ATE284891T1 - Isolierung von einzelsträngigen nukleinsäuren - Google Patents
Isolierung von einzelsträngigen nukleinsäurenInfo
- Publication number
- ATE284891T1 ATE284891T1 AT97902751T AT97902751T ATE284891T1 AT E284891 T1 ATE284891 T1 AT E284891T1 AT 97902751 T AT97902751 T AT 97902751T AT 97902751 T AT97902751 T AT 97902751T AT E284891 T1 ATE284891 T1 AT E284891T1
- Authority
- AT
- Austria
- Prior art keywords
- single stranded
- nucleic acid
- stranded nucleic
- methods
- silica particles
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 5
- 102000039446 nucleic acids Human genes 0.000 title abstract 5
- 108020004707 nucleic acids Proteins 0.000 title abstract 5
- 238000002955 isolation Methods 0.000 title 1
- 239000000463 material Substances 0.000 abstract 8
- 238000000034 method Methods 0.000 abstract 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 3
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 238000000926 separation method Methods 0.000 abstract 2
- 239000007790 solid phase Substances 0.000 abstract 2
- 230000003196 chaotropic effect Effects 0.000 abstract 1
- 239000002738 chelating agent Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 150000002357 guanidines Chemical class 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP96200354 | 1996-02-14 | ||
| PCT/NL1997/000063 WO1997030062A1 (en) | 1996-02-14 | 1997-02-14 | Isolation and amplification of nucleic acid materials |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE284891T1 true ATE284891T1 (de) | 2005-01-15 |
Family
ID=8223661
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT97902751T ATE284891T1 (de) | 1996-02-14 | 1997-02-14 | Isolierung von einzelsträngigen nukleinsäuren |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7238530B2 (de) |
| EP (1) | EP0880537B1 (de) |
| JP (1) | JP3943597B2 (de) |
| KR (1) | KR100483498B1 (de) |
| AT (1) | ATE284891T1 (de) |
| AU (1) | AU723900B2 (de) |
| CA (1) | CA2245888C (de) |
| DE (1) | DE69731939T2 (de) |
| ES (1) | ES2235225T3 (de) |
| WO (1) | WO1997030062A1 (de) |
Families Citing this family (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2965131B2 (ja) | 1995-07-07 | 1999-10-18 | 東洋紡績株式会社 | 核酸結合用磁性担体およびそれを用いる核酸単離方法 |
| US6423536B1 (en) | 1999-08-02 | 2002-07-23 | Molecular Dynamics, Inc. | Low volume chemical and biochemical reaction system |
| EP1276901A2 (de) | 2000-01-13 | 2003-01-22 | Amsterdam Support Diagnostics B.V. | Ein universelles nukleinsäure-amplifikationssystem für nukleinsäuren in einer probe |
| WO2002059353A2 (en) * | 2001-01-26 | 2002-08-01 | Bio S & T | Two-step amplification using a program primer followed by specific primers |
| WO2003046146A2 (en) | 2001-11-28 | 2003-06-05 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
| EP1560926B2 (de) † | 2002-11-08 | 2013-08-21 | STRATEC Molecular GmbH | Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren |
| EP1418241A1 (de) * | 2002-11-08 | 2004-05-12 | PrimaGen Holding B.V. | Methode zur Quantifizierung des Verhältnisses zweier Nukleinsäuresequenzen |
| EP1604040B1 (de) | 2003-03-07 | 2010-10-13 | Rubicon Genomics, Inc. | Amplifikation und analyse von gesamtgenom- und gesamttranskriptom- bibliotheken, die durch ein dns-polymerisierungsverfahren produziert wurden |
| US8206913B1 (en) | 2003-03-07 | 2012-06-26 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process |
| JP4241183B2 (ja) | 2003-05-19 | 2009-03-18 | 株式会社日立ハイテクノロジーズ | 核酸回収方法及び核酸回収キット |
| DE10358137A1 (de) * | 2003-12-12 | 2005-07-07 | Merck Patent Gmbh | Verfahren und Kit zur Isolierung von RNA |
| JP2005218385A (ja) * | 2004-02-06 | 2005-08-18 | Dnaform:Kk | １本鎖ｄｎａを調製するための方法 |
| JP4690656B2 (ja) * | 2004-02-12 | 2011-06-01 | ジーエルサイエンス株式会社 | 核酸の分離精製方法及び分離吸着体 |
| AU2005200670B2 (en) * | 2004-02-20 | 2007-05-03 | F. Hoffmann-La Roche Ag | Adsorption of nucleic acids to a solid phase |
| EP2380993B1 (de) | 2004-03-08 | 2015-12-23 | Rubicon Genomics, Inc. | Verfahren zur Erstellung und Erweiterung von DNA-Bibliotheken zur sensitiven Erkennung und Analyse von DNA-Methylierung |
| EP1589119A1 (de) * | 2004-04-19 | 2005-10-26 | Academisch Medisch Centrum | Detektion von enterovirales RNS und Methode für reverse Transkription von RNS |
| CA2563954A1 (en) * | 2004-04-19 | 2005-10-27 | Academisch Medisch Centrum | Detection of viral nucleic acid and method for reverse transcribing rna |
| AU2005277527A1 (en) * | 2004-08-18 | 2006-03-02 | Preanalytix Gmbh | Additive, method, and article for DNA collection, stabilization, and purification |
| AU2011253981B2 (en) * | 2004-09-02 | 2015-03-26 | Lifeind Ehf. | Two-dimensional strandness- and length-dependent separation of nucleic acid fragments |
| WO2006025074A2 (en) * | 2004-09-02 | 2006-03-09 | Lifeind Ehf. | Twodimensional strandness- and length-dependent separation of nucleic acid fragments |
| DE102004045332B3 (de) * | 2004-09-16 | 2006-03-09 | Macherey, Nagel Gmbh & Co. Handelsgesellschaft | Verfahren zur Isolierung und Trennung von einzel- und doppelsträngigen Nucleinsäuren aus einer diese Nucleinsäuren enthaltenden Probe sowie Trennsäule zur Durchführung dieses Verfahrens |
| US20060166223A1 (en) * | 2005-01-26 | 2006-07-27 | Reed Michael W | DNA purification and analysis on nanoengineered surfaces |
| WO2007018601A1 (en) | 2005-08-02 | 2007-02-15 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction |
| WO2007018602A1 (en) | 2005-08-02 | 2007-02-15 | Rubicon Genomics, Inc. | Isolation of cpg islands by thermal segregation and enzymatic selection-amplification method |
| JP4699868B2 (ja) | 2005-11-04 | 2011-06-15 | 株式会社日立ハイテクノロジーズ | 核酸精製方法及び核酸精製器具 |
| WO2008002882A2 (en) * | 2006-06-26 | 2008-01-03 | Blood Cell Storage, Inc. | Device and method for extraction and analysis of nucleic acids from biological samples |
| WO2009117167A1 (en) * | 2008-01-02 | 2009-09-24 | Blood Cell Storage, Inc. | Devices and processes for nucleic acid extraction |
| EP2247753B1 (de) * | 2008-02-01 | 2014-06-25 | Siemens Healthcare Diagnostics Inc. | Urintransportmittel |
| WO2009134652A1 (en) * | 2008-04-30 | 2009-11-05 | Ge Healthcare Bio-Sciences Corp. | Method for separation of double-stranded and single-stranded nucleic acids |
| DE102008026058A1 (de) | 2008-05-30 | 2009-12-03 | Qiagen Gmbh | Lyse, Binde- und/oder Waschreagenz verwendbar zur Isolierung und/oder Reinigung von Nukleinsäuren |
| KR20110101143A (ko) * | 2008-11-04 | 2011-09-15 | 블러드 셀 스토리지 인코퍼레이티드 | 굴곡 유리 표면 상에서의 핵산 추출 |
| EP2345719A1 (de) | 2010-01-18 | 2011-07-20 | Qiagen GmbH | Verfahren zur Isolierung kleiner RNA |
| EP2407540A1 (de) | 2010-07-15 | 2012-01-18 | Qiagen GmbH | Verfahren zur Reinigung von Nukleinsäure |
| JP6096660B2 (ja) | 2010-09-02 | 2017-03-15 | キアゲン ゲーエムベーハー | 低分子標的核酸を含む標的核酸を高収量で単離するための方法 |
| US8629264B2 (en) | 2011-05-19 | 2014-01-14 | Blood Cell Storage, Inc. | Gravity flow fluidic device for nucleic acid extraction |
| EP3789500A1 (de) | 2011-08-12 | 2021-03-10 | QIAGEN GmbH | Verfahren zur isolierung von nukleinsäuren |
| WO2013037401A1 (en) | 2011-09-13 | 2013-03-21 | Qiagen Gmbh | Method for isolating nucleic acids from a veterinary whole blood sample |
| ES2574956T3 (es) | 2011-09-26 | 2016-06-23 | Preanalytix Gmbh | Estabilización y aislamiento de ácidos nucleicos extracelulares |
| CN113355321A (zh) | 2011-09-26 | 2021-09-07 | 凯杰有限公司 | 用于分离细胞外核酸的快速方法 |
| ES2971646T3 (es) | 2011-09-26 | 2024-06-06 | Preanalytix Gmbh | Estabilización y aislamiento de ácidos nucleicos extracelulares |
| US11021733B2 (en) | 2011-09-26 | 2021-06-01 | Qiagen Gmbh | Stabilization and isolation of extracellular nucleic acids |
| EP2888363B1 (de) | 2012-08-21 | 2018-06-06 | Qiagen GmbH | Verfahren zur isolierung von nukleinsäuren aus einer stabilisierten probe mit formaldehydfreigabe |
| WO2014029792A1 (en) | 2012-08-21 | 2014-02-27 | Qiagen Gmbh | Virus particle stabilisation and method for isolating viral nucleic acids |
| WO2014033326A1 (en) | 2012-09-03 | 2014-03-06 | Qiagen Gmbh | Method for isolating rna including small rna with high yield |
| WO2014044724A1 (en) | 2012-09-18 | 2014-03-27 | Qiagen Gmbh | Method and kit for preparing a target rna depleted sample |
| CA2884915C (en) | 2012-09-25 | 2022-05-17 | Qiagen Gmbh | Stabilisation of biological samples |
| WO2014090838A1 (en) | 2012-12-11 | 2014-06-19 | Qiagen Gmbh | Preparation of silica particles |
| WO2014146782A1 (en) | 2013-03-18 | 2014-09-25 | Qiagen Gmbh | Stabilization and isolation of extracellular nucleic acids |
| EP2976424B1 (de) | 2013-03-18 | 2018-10-03 | Qiagen GmbH | Stabilisierung von biologischen proben |
| CN106132200B (zh) | 2014-03-18 | 2020-10-30 | 凯杰有限公司 | 胞外核酸的固定和分离 |
| EP2940136A1 (de) | 2014-04-30 | 2015-11-04 | QIAGEN GmbH | Verfahren zur Isolierung von Poly(A)-Nukleinsäuren |
| ZA201608812B (en) | 2014-06-26 | 2019-08-28 | Janssen Vaccines & Prevention Bv | Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau |
| BR112016029579A2 (pt) | 2014-06-26 | 2017-08-22 | Janssen Vaccines & Prevention Bv | anticorpos e fragmentos de ligação ao antígeno que se ligam especificamente à proteína tau associada a microtúbulos |
| CN106715714B (zh) * | 2014-10-17 | 2021-11-09 | 深圳华大智造科技股份有限公司 | 一种用于核酸随机片段化的引物及核酸随机片段化方法 |
| JP6787916B2 (ja) | 2015-06-10 | 2020-11-18 | キアゲン ゲーエムベーハー | アニオン交換粒子を使用する細胞外核酸を単離する方法 |
| US10214739B2 (en) * | 2015-10-30 | 2019-02-26 | Axagarius Gmbh & Co. Kg | RNA binding solution |
| CN114457068B (zh) | 2015-11-20 | 2025-03-14 | 普瑞阿那利提克斯有限公司 | 用于稳定细胞外核酸的已灭菌组合物的制备方法 |
| WO2019063071A1 (en) | 2017-09-27 | 2019-04-04 | Qiagen Gmbh | METHOD FOR HIGH-PERFORMANCE RNA ISOLATION |
| EP4397771A3 (de) | 2019-01-04 | 2024-10-16 | PreAnalytiX GmbH | Urinstabilisierung |
| WO2021211849A1 (en) * | 2020-04-16 | 2021-10-21 | Chhalliyil Pradheep | Rapid extraction and purification of rna |
| WO2025203643A1 (ja) * | 2024-03-29 | 2025-10-02 | 株式会社日立ハイテク | 一本鎖核酸の検出方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| LU82089A1 (fr) * | 1980-01-16 | 1981-09-10 | Sipac | Procede de preparation d'un echangeur de cations et procede d'utilisation de cet echangeur pour l'extraction de metaux de liquides |
| US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US5075430A (en) * | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
| NL8900725A (nl) * | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5043272A (en) * | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| IL101356A (en) * | 1991-04-03 | 1996-08-04 | Perkin Elmer Corp | Solvents for the separation of nucleic acids by replacing anions |
| US5155018A (en) * | 1991-07-10 | 1992-10-13 | Hahnemann University | Process and kit for isolating and purifying RNA from biological sources |
| AU7375794A (en) * | 1993-07-28 | 1995-02-28 | Akzo Nobel N.V. | Process for isolating nucleic acid from gram positive microorganisms |
| CA2170604C (en) * | 1993-08-30 | 2007-03-13 | Vikas V. Padhye | Nucleic acid purification compositions and methods |
| US6180778B1 (en) * | 1994-02-11 | 2001-01-30 | Qiagen Gmbh | Process for the separation of double-stranded/single-stranded nucleic acid structures |
| WO1995028409A1 (en) | 1994-04-14 | 1995-10-26 | The Rockefeller University | Process, apparatus and reagents for isolating cellular components |
| JP3761573B2 (ja) * | 1994-06-14 | 2006-03-29 | インヴィテーク ゲーエムベーハー | 極度に少量でかつ非常に強く汚染された非常に種々の出発物質から核酸を単離および精製する一般的方法 |
| US6037465A (en) * | 1994-06-14 | 2000-03-14 | Invitek Gmbh | Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials |
-
1997
- 1997-02-14 AT AT97902751T patent/ATE284891T1/de not_active IP Right Cessation
- 1997-02-14 EP EP97902751A patent/EP0880537B1/de not_active Expired - Lifetime
- 1997-02-14 CA CA002245888A patent/CA2245888C/en not_active Expired - Lifetime
- 1997-02-14 WO PCT/NL1997/000063 patent/WO1997030062A1/en not_active Ceased
- 1997-02-14 AU AU16766/97A patent/AU723900B2/en not_active Expired
- 1997-02-14 DE DE69731939T patent/DE69731939T2/de not_active Expired - Lifetime
- 1997-02-14 ES ES97902751T patent/ES2235225T3/es not_active Expired - Lifetime
- 1997-02-14 KR KR10-1998-0706258A patent/KR100483498B1/ko not_active Expired - Lifetime
- 1997-02-14 JP JP52922197A patent/JP3943597B2/ja not_active Expired - Lifetime
-
2001
- 2001-01-12 US US09/760,085 patent/US7238530B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO1997030062A1 (en) | 1997-08-21 |
| AU1676697A (en) | 1997-09-02 |
| JP3943597B2 (ja) | 2007-07-11 |
| EP0880537B1 (de) | 2004-12-15 |
| ES2235225T3 (es) | 2005-07-01 |
| JP2000505295A (ja) | 2000-05-09 |
| DE69731939T2 (de) | 2005-12-22 |
| KR19990082522A (ko) | 1999-11-25 |
| KR100483498B1 (ko) | 2005-07-18 |
| EP0880537A1 (de) | 1998-12-02 |
| CA2245888C (en) | 2008-12-23 |
| DE69731939D1 (de) | 2005-01-20 |
| US20010021518A1 (en) | 2001-09-13 |
| US7238530B2 (en) | 2007-07-03 |
| CA2245888A1 (en) | 1997-08-21 |
| AU723900B2 (en) | 2000-09-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |