ATE286124T1 - Verfahren zur herstellung von polynukleotidvarianten - Google Patents

Verfahren zur herstellung von polynukleotidvarianten

Info

Publication number
ATE286124T1
ATE286124T1 AT02790188T AT02790188T ATE286124T1 AT E286124 T1 ATE286124 T1 AT E286124T1 AT 02790188 T AT02790188 T AT 02790188T AT 02790188 T AT02790188 T AT 02790188T AT E286124 T1 ATE286124 T1 AT E286124T1
Authority
AT
Austria
Prior art keywords
pcr
polynucleotides
mutated
polynucleotide variants
series
Prior art date
Application number
AT02790188T
Other languages
English (en)
Inventor
Roelof Ary Lans Bovenberg
Richard Kerkman
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26076965&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE286124(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of ATE286124T1 publication Critical patent/ATE286124T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT02790188T 2001-07-23 2002-07-23 Verfahren zur herstellung von polynukleotidvarianten ATE286124T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01202821 2001-07-23
EP01203457 2001-09-11
PCT/EP2002/008225 WO2003010183A2 (en) 2001-07-23 2002-07-23 Process for preparing variant polynucleotides

Publications (1)

Publication Number Publication Date
ATE286124T1 true ATE286124T1 (de) 2005-01-15

Family

ID=26076965

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02790188T ATE286124T1 (de) 2001-07-23 2002-07-23 Verfahren zur herstellung von polynukleotidvarianten

Country Status (7)

Country Link
US (1) US7402383B2 (de)
EP (1) EP1409667B2 (de)
AT (1) ATE286124T1 (de)
AU (1) AU2002333269A1 (de)
DE (1) DE60202478T3 (de)
DK (1) DK1409667T4 (de)
WO (1) WO2003010183A2 (de)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7402383B2 (en) 2001-07-23 2008-07-22 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
EP2123772A1 (de) 2008-04-29 2009-11-25 DSM IP Assets B.V. Beta-Lactamantibiotikum produzierende Stämme
EP2123767A1 (de) 2008-05-20 2009-11-25 DSM IP Assets B.V. Herstellung von Epsilon-Caprolactam mittels Lysinzyklisierung
EP2123768A1 (de) 2008-05-20 2009-11-25 DSM IP Assets B.V. Herstellung von (Z)-6,7-Dihydro-1H-azepin-2(5H)-on
EP2130918A1 (de) * 2008-06-05 2009-12-09 C-Lecta GmbH Verfahren zur Erzeugung einer Varianten-Bibliothek von DNA-Sequenzen
US20090312196A1 (en) * 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
CN102131921A (zh) 2008-08-05 2011-07-20 帝斯曼知识产权资产管理有限公司 生产己二酰基-7-adca的菌株
TW201037078A (en) 2009-03-11 2010-10-16 Dsm Ip Assets Bv Preparation of alpha-ketopimelic acid
BRPI1015536A2 (pt) 2009-04-24 2019-07-02 Dsm Ip Assets Bv polipeptídeo de degradação de carboidratos e usos do mesmo
US9133448B2 (en) 2010-02-11 2015-09-15 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
BR112012033404B1 (pt) 2010-06-29 2021-05-18 Dsm Ip Assets B.V. célula recombinante, método para a preparação de um polipeptídeo, composição, método para o tratamento de um substrato que compreende material de carboidrato e uso de um polipeptídeo
CA2802128A1 (en) 2010-06-29 2012-01-05 Margot Elisabeth Francoise Schooneveld-Bergmans Polypeptide having beta-glucosidase activity and uses thereof
DK2588494T3 (en) 2010-06-29 2018-06-14 Dsm Ip Assets Bv POLYPEPTIDE WITH BETA-GLUCOSIDASE ACTIVITY AND APPLICATIONS THEREOF
WO2012000891A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
US9175050B2 (en) 2010-06-29 2015-11-03 Dsm Ip Assets B.V. Polypeptide having swollenin activity and uses thereof
CA2803513A1 (en) 2010-06-29 2012-01-05 Margot Elisabeth Francoise Schooneveld-Bergmans Polypeptide having acetyl xylan esterase activity and uses thereof
WO2012031910A2 (en) 2010-09-10 2012-03-15 Dsm Ip Assets B.V. Method for preparing alpha-ketopimelic acid by c1-elongation
MX355028B (es) 2012-01-23 2018-03-28 Dsm Ip Assets Bv Produccion de diterpeno.
CN105073986A (zh) 2013-02-04 2015-11-18 帝斯曼知识产权资产管理有限公司 碳水化合物降解多肽及其用途
BR112015029580A2 (pt) 2013-05-31 2017-09-19 Dsm Ip Assets Bv Microrganismo recombinante, processo para a preparação de um diterpeno ou diterpeno glicosilado, diterpeno ou diterpeno glicosilado, gêneros alimentícios, alimentos para animais ou bebidas e método para converter um primeiro diterpeno glicosilado em um segundo diterpeno glicosilado
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
BR112016000745B1 (pt) 2013-07-15 2021-01-05 Dsm Ip Assets B.V. processo para a preparação de rebaudiosídeo m
DK3027632T3 (da) 2013-07-31 2019-06-17 Dsm Ip Assets Bv Indvinding af steviolglycosider fra et fermenteringsmedium
US10829748B2 (en) 2016-06-10 2020-11-10 Dsm Ip Assets B.V. Mutant lipase and use thereof
US20210246519A1 (en) * 2018-05-04 2021-08-12 The Regents Of The University Of California Spiked primers for enrichment of pathogen nucleic acids among background of nucleic acids
US20230250406A1 (en) 2018-05-18 2023-08-10 Dsm Ip Assets B.V. Mutant lipase and use thereof
WO2019219903A2 (en) 2018-05-18 2019-11-21 Dsm Ip Assets B.V. Mutant lipase and use thereof
CN109750032A (zh) * 2019-02-28 2019-05-14 尚科生物医药（上海）有限公司 一种构建基因多点突变和进化的方法
EP3766982A1 (de) 2019-07-18 2021-01-20 Delft Advanced Biofuels B.V. Integriertes system zur biokatalytischen herstellung und rückgewinnung einer organischen substanz
US20250290081A1 (en) 2020-10-22 2025-09-18 Dsm Ip Assets B.V. Microorganisms for diterpene production
CN121127603A (zh) 2023-04-20 2025-12-12 帝斯曼知识产权资产管理有限公司 用于产生罗汉果苷的菌株和方法
WO2025144795A1 (en) 2023-12-28 2025-07-03 Dsm Ip Assets B.V. Cells and methods for the production of glycosylated compounds

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DK0843725T3 (da) 1995-08-11 2002-08-12 Novozymes As Fremgangmåde til fremstilling af polypeptidvarianter
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6242222B1 (en) * 1996-06-07 2001-06-05 Massachusetts Institute Of Technology Programmed sequential mutagenesis
AU743305C (en) 1997-01-17 2006-03-30 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
NZ337910A (en) 1997-03-18 2001-10-26 Novo Nordisk As Shuffling of heterologous DNA sequences using conserved regions
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6767701B1 (en) * 1998-10-26 2004-07-27 Novozymes A/S Methods of constructing and screening a DNA library of interest in filamentous fungal cells
WO2000042560A2 (en) * 1999-01-19 2000-07-20 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides
WO2001029212A1 (en) * 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Methods for chimeragenesis of whole genomes or large polynucleotides
ATE321850T1 (de) * 2001-07-23 2006-04-15 Dsm Ip Assets Bv Verfahren zur herstellung von polynucleotivarianten
US7402383B2 (en) 2001-07-23 2008-07-22 Dsm Ip Assets B.V. Process for preparing variant polynucleotides

Also Published As

Publication number Publication date
EP1409667B1 (de) 2004-12-29
DE60202478T3 (de) 2010-01-07
WO2003010183A3 (en) 2003-11-13
DE60202478D1 (de) 2005-02-03
DE60202478T2 (de) 2005-12-29
US20050130141A1 (en) 2005-06-16
DK1409667T3 (da) 2005-04-25
EP1409667B2 (de) 2009-06-10
AU2002333269A1 (en) 2003-02-17
DK1409667T4 (da) 2009-09-07
WO2003010183A2 (en) 2003-02-06
EP1409667A2 (de) 2004-04-21
US7402383B2 (en) 2008-07-22

Similar Documents

Publication Publication Date Title
DE60202478D1 (de) Verfahren zur herstellung von polynukleotidvarianten
DE69824716D1 (de) Methode zur sequenzierung von nukleinsäuren
DE69636080D1 (de) Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren
DE69433487D1 (de) Methoden und zusammensetzungen zur effizienten nukleinsaeuresequenzierung
EP2272952B8 (de) Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität
BR9510003B1 (pt) moléculas não naturais de dna enzimático e método de preparação.
DE69837913D1 (de) Verfahren zur vervielfältigung von nukleinsäure
ATE205878T1 (de) Verfahren zur herstellung von polynucleotiden mit erwünschten eigenschaften mittels wiederholter selektion und rekombination
WO2004072294A3 (en) Methods and means for nucleic acid sequencing
SI2258847T1 (sl) Nadaljnje nove oblike molekul interferenčne RNA
WO2003033724A3 (en) Nucleic acid amplification
ATE277200T1 (de) Verfahren zur herstellung von biopolymeren mit veränderten eigenschaften
DE60214864D1 (de) Verfahren zur herstellung von nukleinsäuren bestehend aus stochastisch kombinierten anteilen von ausgangsnukleinsäuren
ATE431407T1 (de) Verfahren zur herstellung von dna
WO2003102232A3 (de) Vermehrung von ribonukleinsäuren
WO2003097834A3 (en) A method for in vitro molecular evolution of protein function
DE602004011546D1 (de) Verfahren zur sequenz-saturations-mutagenese (sesam)
WO2003100058A3 (de) Verfahren zur erzeugung von polynukleotidmolekülen
Murakami et al. Random insertion and deletion mutagenesis for construction of protein library containing nonnatural amino acids
ATE261968T1 (de) Ein verfahren zur herstellung von 2,6- dichloropurin
WO2024178112A3 (en) Use of destabilizing nucleotide triphosphates to reduce amplicon folding and tm
WO2005074470A3 (en) Kits and methods for making large recombinant polynucleotides

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1409667

Country of ref document: EP

UEP Publication of translation of european patent specification

Ref document number: 1409667

Country of ref document: EP