ATE286124T1 - Verfahren zur herstellung von polynukleotidvarianten - Google Patents

Verfahren zur herstellung von polynukleotidvarianten

Info

Publication number
ATE286124T1
ATE286124T1 AT02790188T AT02790188T ATE286124T1 AT E286124 T1 ATE286124 T1 AT E286124T1 AT 02790188 T AT02790188 T AT 02790188T AT 02790188 T AT02790188 T AT 02790188T AT E286124 T1 ATE286124 T1 AT E286124T1
Authority
AT
Austria
Prior art keywords
pcr
polynucleotides
mutated
polynucleotide variants
series
Prior art date
Application number
AT02790188T
Other languages
English (en)
Inventor
Roelof Ary Lans Bovenberg
Richard Kerkman
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26076965&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE286124(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of ATE286124T1 publication Critical patent/ATE286124T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT02790188T 2001-07-23 2002-07-23 Verfahren zur herstellung von polynukleotidvarianten ATE286124T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01202821 2001-07-23
EP01203457 2001-09-11
PCT/EP2002/008225 WO2003010183A2 (en) 2001-07-23 2002-07-23 Process for preparing variant polynucleotides

Publications (1)

Publication Number Publication Date
ATE286124T1 true ATE286124T1 (de) 2005-01-15

Family

ID=26076965

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02790188T ATE286124T1 (de) 2001-07-23 2002-07-23 Verfahren zur herstellung von polynukleotidvarianten

Country Status (7)

Country Link
US (1) US7402383B2 (de)
EP (1) EP1409667B2 (de)
AT (1) ATE286124T1 (de)
AU (1) AU2002333269A1 (de)
DE (1) DE60202478T3 (de)
DK (1) DK1409667T4 (de)
WO (1) WO2003010183A2 (de)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7402383B2 (en) 2001-07-23 2008-07-22 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
EP2123772A1 (de) 2008-04-29 2009-11-25 DSM IP Assets B.V. Beta-Lactamantibiotikum produzierende Stämme
EP2123768A1 (de) 2008-05-20 2009-11-25 DSM IP Assets B.V. Herstellung von (Z)-6,7-Dihydro-1H-azepin-2(5H)-on
EP2123767A1 (de) 2008-05-20 2009-11-25 DSM IP Assets B.V. Herstellung von Epsilon-Caprolactam mittels Lysinzyklisierung
EP2130918A1 (de) * 2008-06-05 2009-12-09 C-Lecta GmbH Verfahren zur Erzeugung einer Varianten-Bibliothek von DNA-Sequenzen
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
EP2310487A2 (de) 2008-08-05 2011-04-20 DSM IP Assets B.V. Adipoyl-7-adca produzierende stämme
AU2010221863A1 (en) 2009-03-11 2011-10-06 Dsm Ip Assets B.V. Preparation of adipic acid
CA2758396C (en) 2009-04-24 2018-06-12 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
US9133448B2 (en) 2010-02-11 2015-09-15 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
ES2672125T3 (es) 2010-06-29 2018-06-12 Dsm Ip Assets B.V. Polipéptido que tiene actividad beta-glucosidasa y usos del mismo
EP2588493A1 (de) 2010-06-29 2013-05-08 DSM IP Assets B.V. Polypeptid mit swolleninaktivität und verwendungen davon
EP2588601A1 (de) 2010-06-29 2013-05-08 DSM IP Assets B.V. Polypeptid mit kohlenhydratabbauender wirkung und seine verwendung
MX2012015144A (es) 2010-06-29 2013-03-05 Dsm Ip Assets Bv Polipeptido que tiene actividad de acetil-xilan-esterasa y usos del mismo.
DK2588603T3 (en) 2010-06-29 2017-07-03 Dsm Ip Assets Bv POLYPEPTIDE THAT HAS OR CONTRIBUTES TO CARBOHYDRATE MATERIAL DEGRADING ACTIVITIES AND APPLICATIONS THEREOF
WO2012000886A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
WO2012031910A2 (en) 2010-09-10 2012-03-15 Dsm Ip Assets B.V. Method for preparing alpha-ketopimelic acid by c1-elongation
AU2013211605B2 (en) 2012-01-23 2017-04-06 Dsm Ip Assets B.V. Diterpene production
PL3686280T3 (pl) 2013-02-04 2022-09-19 Dsm Ip Assets B.V. Polipeptyd degradujący węglowodany i jego zastosowania
US10689681B2 (en) 2013-05-31 2020-06-23 Dsm Ip Assets B.V. Microorganisms for diterpene production
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
EP3021689B1 (de) 2013-07-15 2021-03-24 DSM IP Assets B.V. Herstellung von diterpen
AU2014298420B2 (en) 2013-07-31 2018-10-04 Dsm Ip Assets B.V. Recovery of steviol glycosides
WO2017211930A1 (en) 2016-06-10 2017-12-14 Dsm Ip Assets B.V. Mutant lipase and use thereof
WO2019213624A1 (en) * 2018-05-04 2019-11-07 The Regents Of The University Of California Spiked primers for enrichment of pathogen nucleic acids among background of nucleic acids
KR20210013585A (ko) 2018-05-18 2021-02-04 디에스엠 아이피 어셋츠 비.브이. 돌연변이체 리파아제 및 이의 용도
US20230250406A1 (en) 2018-05-18 2023-08-10 Dsm Ip Assets B.V. Mutant lipase and use thereof
CN109750032A (zh) * 2019-02-28 2019-05-14 尚科生物医药（上海）有限公司 一种构建基因多点突变和进化的方法
EP3766982A1 (de) 2019-07-18 2021-01-20 Delft Advanced Biofuels B.V. Integriertes system zur biokatalytischen herstellung und rückgewinnung einer organischen substanz
AU2021367159A1 (en) 2020-10-22 2023-05-04 Cargill, Incorporated Microorganisms for diterpene production
WO2024218244A1 (en) 2023-04-20 2024-10-24 Dsm Ip Assets B.V. Strains and methods for the production of mogrosides
WO2025144795A1 (en) 2023-12-28 2025-07-03 Dsm Ip Assets B.V. Cells and methods for the production of glycosylated compounds

Family Cites Families (13)

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Publication number Priority date Publication date Assignee Title
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DE69620766T2 (de) 1995-08-11 2004-11-18 Novozymes A/S Verfahren zur herstellung von polypeptidabkömmlingen
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
JP2000512142A (ja) * 1996-06-07 2000-09-19 マサチューセッツ インスティチュート オブ テクノロジー プログラムされた連続的変異誘発
KR100570935B1 (ko) 1997-01-17 2006-04-13 맥시겐, 인크. 반복적 서열 재조합에 의한 전세포 및 유기체의 개량 방법
AU738461B2 (en) 1997-03-18 2001-09-20 Novozymes A/S Shuffling of heterologous DNA sequences
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6767701B1 (en) * 1998-10-26 2004-07-27 Novozymes A/S Methods of constructing and screening a DNA library of interest in filamentous fungal cells
ES2341217T3 (es) 1999-01-19 2010-06-17 Maxygen, Inc. Recombinacion de acidos nucleicos mediada por oligonucleotidos.
WO2001029212A1 (en) 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Methods for chimeragenesis of whole genomes or large polynucleotides
US7402383B2 (en) 2001-07-23 2008-07-22 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
US20050130140A1 (en) * 2001-07-23 2005-06-16 Bovenberg Roelof A.L. Process for preparing variant polynucleotides

Also Published As

Publication number Publication date
EP1409667B2 (de) 2009-06-10
DE60202478T3 (de) 2010-01-07
EP1409667B1 (de) 2004-12-29
EP1409667A2 (de) 2004-04-21
DE60202478D1 (de) 2005-02-03
DE60202478T2 (de) 2005-12-29
DK1409667T4 (da) 2009-09-07
WO2003010183A3 (en) 2003-11-13
US20050130141A1 (en) 2005-06-16
US7402383B2 (en) 2008-07-22
AU2002333269A1 (en) 2003-02-17
DK1409667T3 (da) 2005-04-25
WO2003010183A2 (en) 2003-02-06

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