DK1015575T3 - Shuffling af heterologe DNA-sekvenser - Google Patents

Shuffling af heterologe DNA-sekvenser

Info

Publication number
DK1015575T3
DK1015575T3 DK98907912.4T DK98907912T DK1015575T3 DK 1015575 T3 DK1015575 T3 DK 1015575T3 DK 98907912 T DK98907912 T DK 98907912T DK 1015575 T3 DK1015575 T3 DK 1015575T3
Authority
DK
Denmark
Prior art keywords
shuffling
conserved region
fragments
interest
identification
Prior art date
Application number
DK98907912.4T
Other languages
English (en)
Inventor
Joel R Cherry
Torben Vedel Borchert
Titus Kretzschmar
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26063733&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1015575(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK1015575T3 publication Critical patent/DK1015575T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DK98907912.4T 1997-03-18 1998-03-18 Shuffling af heterologe DNA-sekvenser DK1015575T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK30497 1997-03-18
DK43297 1997-04-17
PCT/DK1998/000105 WO1998041623A1 (en) 1997-03-18 1998-03-18 Shuffling of heterologous dna sequences

Publications (1)

Publication Number Publication Date
DK1015575T3 true DK1015575T3 (da) 2010-08-23

Family

ID=26063733

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98907912.4T DK1015575T3 (da) 1997-03-18 1998-03-18 Shuffling af heterologe DNA-sekvenser

Country Status (10)

Country Link
EP (1) EP1015575B1 (da)
JP (1) JP2001516216A (da)
AT (1) ATE468394T1 (da)
AU (1) AU738461B2 (da)
CA (1) CA2284097A1 (da)
DE (1) DE69841674D1 (da)
DK (1) DK1015575T3 (da)
IL (1) IL131986A0 (da)
NZ (1) NZ337910A (da)
WO (1) WO1998041623A1 (da)

Families Citing this family (60)

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US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
EP1559788A1 (en) 1997-04-09 2005-08-03 Danisco A/S Use lipase for improving doughs and baked products
DK1036198T3 (da) 1997-12-08 2013-01-02 California Inst Of Techn Fremgangsmåde til fremstilling af polynukleotid- og polypeptidsekvenser
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
NZ507591A (en) 1998-05-01 2002-03-28 Maxygen Inc Optimization of pest resistance genes using DNA shuffling
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US6846655B1 (en) 1998-06-29 2005-01-25 Phylos, Inc. Methods for generating highly diverse libraries
WO2000009682A1 (en) 1998-08-12 2000-02-24 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
CA2345203A1 (en) 1998-10-07 2000-04-13 Maxygen Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
EP1129184A1 (en) 1998-11-10 2001-09-05 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimation of plant phenotypes
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
EP1130093A1 (en) 1999-01-19 2001-09-05 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
JP2003524394A (ja) 1999-02-11 2003-08-19 マキシジェン， インコーポレイテッド ハイスループット質量分析法
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
WO2000061731A2 (en) * 1999-04-13 2000-10-19 Maxygen, Inc. Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
IL150291A0 (en) 2000-01-11 2002-12-01 Maxygen Inc Integrated systems and methods for diversity generation and screening
US6248541B1 (en) 2000-04-21 2001-06-19 Genencor International, Inc. Screening under nutrient limited conditions
US6534292B1 (en) 2000-05-08 2003-03-18 Genencor International, Inc. Methods for forming recombined nucleic acids
EP1283877A2 (en) * 2000-05-23 2003-02-19 California Institute Of Technology Gene recombination and hybrid protein development
AU2001268716A1 (en) 2000-06-23 2002-01-08 Maxygen, Inc. Novel chimeric promoters
CA2411828A1 (en) 2000-06-23 2002-01-03 Maxygen, Inc. Novel co-stimulatory molecules
EP2287296A1 (en) 2000-06-26 2011-02-23 Novozymes A/S Lipolytic enzyme
US6994963B1 (en) 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
AUPQ974900A0 (en) * 2000-08-29 2000-09-21 Macquarie Research Limited Degenerate oligonucleotide gene-shuffling
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
ES2284897T3 (es) 2001-05-18 2007-11-16 Danisco A/S Procedimiento para la preparacion de una masa con una enzima.
ES2533923T3 (es) 2001-06-26 2015-04-16 Novozymes A/S Polipéptidos con actividad de celobiohidrolasa I y polinucleótidos que codifican los mismos
WO2003010183A2 (en) 2001-07-23 2003-02-06 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
US7262012B2 (en) 2002-05-17 2007-08-28 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations
ES2534903T3 (es) 2002-12-20 2015-04-30 Novozymes A/S Variantes de galactanasas
EP1578964B2 (en) 2002-12-20 2013-09-04 Novozymes A/S Polypeptides having cellobiohydrolase ii activity and polynucleotides encoding same
MXPA05007653A (es) 2003-01-17 2005-09-30 Danisco Metodo.
DK1622921T3 (da) 2003-05-02 2010-09-13 Novozymes Inc Varianter af beta-glucosidaser
CN103088001A (zh) 2003-08-25 2013-05-08 诺维信股份有限公司 糖苷水解酶的变体
EP2308966A1 (en) 2003-10-10 2011-04-13 Novozymes A/S Protease variants
JP4891220B2 (ja) 2004-02-25 2012-03-07 ノボザイムス アクティーゼルスカブ 菌類細胞壁分解酵素
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
US9045748B2 (en) 2004-05-27 2015-06-02 Novozymes, Inc. Methods for transforming and expression screening of filamentous fungal cells with a DNA library
CN102533440B (zh) 2004-07-16 2017-09-19 杜邦营养生物科学有限公司 用酶使油脱胶的方法
CN107864658A (zh) 2015-04-07 2018-03-30 诺维信公司 用于选择具有脂肪酶活性的酶的方法
CN107667177A (zh) 2015-04-07 2018-02-06 诺维信公司 用于选择具有增强活性的酶的方法
EP3440207A1 (en) 2016-04-07 2019-02-13 Novozymes A/S Methods for selecting enzymes having protease activity

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination

Also Published As

Publication number Publication date
AU6611698A (en) 1998-10-12
JP2001516216A (ja) 2001-09-25
EP1015575A1 (en) 2000-07-05
DE69841674D1 (de) 2010-07-01
EP1015575B1 (en) 2010-05-19
AU738461B2 (en) 2001-09-20
WO1998041623A1 (en) 1998-09-24
NZ337910A (en) 2001-10-26
IL131986A0 (en) 2001-03-19
CA2284097A1 (en) 1998-09-24
ATE468394T1 (de) 2010-06-15

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