ATE288964T1 - Methoden für externe kontrollen zur nukleinsäure amplifizierung - Google Patents
Methoden für externe kontrollen zur nukleinsäure amplifizierungInfo
- Publication number
- ATE288964T1 ATE288964T1 AT01964412T AT01964412T ATE288964T1 AT E288964 T1 ATE288964 T1 AT E288964T1 AT 01964412 T AT01964412 T AT 01964412T AT 01964412 T AT01964412 T AT 01964412T AT E288964 T1 ATE288964 T1 AT E288964T1
- Authority
- AT
- Austria
- Prior art keywords
- target
- external control
- primers
- nucleic acid
- acid amplification
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 5
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 5
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 4
- 102000040430 polynucleotide Human genes 0.000 abstract 4
- 239000002157 polynucleotide Substances 0.000 abstract 4
- 239000000523 sample Substances 0.000 abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- 238000005259 measurement Methods 0.000 abstract 2
- 108091093088 Amplicon Proteins 0.000 abstract 1
- 101710163270 Nuclease Proteins 0.000 abstract 1
- 239000012807 PCR reagent Substances 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 230000001404 mediated effect Effects 0.000 abstract 1
- 239000013642 negative control Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 102000054765 polymorphisms of proteins Human genes 0.000 abstract 1
- 239000013641 positive control Substances 0.000 abstract 1
- 238000010791 quenching Methods 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/645,959 US6358679B1 (en) | 2000-08-24 | 2000-08-24 | Methods for external controls for nucleic acid amplification |
| PCT/US2001/026499 WO2002016648A2 (en) | 2000-08-24 | 2001-08-23 | Methods for external controls for nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE288964T1 true ATE288964T1 (de) | 2005-02-15 |
Family
ID=24591151
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01964412T ATE288964T1 (de) | 2000-08-24 | 2001-08-23 | Methoden für externe kontrollen zur nukleinsäure amplifizierung |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6358679B1 (de) |
| EP (1) | EP1339870B1 (de) |
| JP (1) | JP2004507248A (de) |
| AT (1) | ATE288964T1 (de) |
| AU (1) | AU8526701A (de) |
| CA (1) | CA2386913A1 (de) |
| DE (1) | DE60108897T2 (de) |
| WO (1) | WO2002016648A2 (de) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020197630A1 (en) * | 2001-04-12 | 2002-12-26 | Knapp Michael R. | Systems and methods for high throughput genetic analysis |
| GB0119274D0 (en) * | 2001-08-08 | 2001-10-03 | Univ Durham | Fusion proteins for insect control |
| US7172865B2 (en) | 2001-08-13 | 2007-02-06 | Saint Louis University | Rapid and sensitive assay for the detection and quantification of coregulators of nucleic acid binding factors |
| US6544746B2 (en) * | 2001-08-13 | 2003-04-08 | St. Louis University | Rapid and sensitive proximity-based assay for the detection and quantification of DNA binding proteins |
| US20030082606A1 (en) * | 2001-09-04 | 2003-05-01 | Lebo Roger V. | Optimizing genome-wide mutation analysis of chromosomes and genes |
| WO2003102179A1 (en) * | 2002-05-31 | 2003-12-11 | Kankyo Engineering Co., Ltd. | Novel method of assyaing nucleic acid using labeled nucleotide |
| ATE526418T1 (de) * | 2002-08-05 | 2011-10-15 | Quanta Biosciences Inc | Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren |
| US20070065830A1 (en) * | 2002-09-04 | 2007-03-22 | Children's Hospital Medical Center Of Akron, Inc. | Cloning multiple control sequences into chromosomes or into artificial centromeres |
| US20040138821A1 (en) * | 2002-09-06 | 2004-07-15 | Affymetrix, Inc. A Corporation Organized Under The Laws Of Delaware | System, method, and computer software product for analysis and display of genotyping, annotation, and related information |
| WO2004055709A2 (en) * | 2002-12-13 | 2004-07-01 | Applera Corporation | Methods for identifying, viewing, and analyzing syntenic and orthologous genomic regions between two or more species |
| EP1606419A1 (de) | 2003-03-18 | 2005-12-21 | Quantum Genetics Ireland Limited | Systeme und methoden zur erhohung der milchproduktion in tieren |
| US20050038776A1 (en) * | 2003-08-15 | 2005-02-17 | Ramin Cyrus | Information system for biological and life sciences research |
| US20060024690A1 (en) * | 2003-09-19 | 2006-02-02 | Kao H P | Normalization of data using controls |
| US20050221357A1 (en) * | 2003-09-19 | 2005-10-06 | Mark Shannon | Normalization of gene expression data |
| WO2005029041A2 (en) * | 2003-09-19 | 2005-03-31 | Applera Corporation | High density sequence detection methods and apparatus |
| US7332280B2 (en) * | 2003-10-14 | 2008-02-19 | Ronald Levy | Classification of patients having diffuse large B-cell lymphoma based upon gene expression |
| WO2005112544A2 (en) | 2004-02-19 | 2005-12-01 | The Governors Of The University Of Alberta | Leptin promoter polymorphisms and uses thereof |
| US20050244837A1 (en) * | 2004-04-28 | 2005-11-03 | Cepheid | Method and device for sample preparation control |
| US20050255485A1 (en) * | 2004-05-14 | 2005-11-17 | Livak Kenneth J | Detection of gene duplications |
| US7575863B2 (en) * | 2004-05-28 | 2009-08-18 | Applied Biosystems, Llc | Methods, compositions, and kits comprising linker probes for quantifying polynucleotides |
| US20060003337A1 (en) * | 2004-06-30 | 2006-01-05 | John Brandis | Detection of small RNAS |
| US7642055B2 (en) * | 2004-09-21 | 2010-01-05 | Applied Biosystems, Llc | Two-color real-time/end-point quantitation of microRNAs (miRNAs) |
| US7405044B2 (en) * | 2004-10-08 | 2008-07-29 | Reliagene Technologies Inc. | Multiplex PCR for simultaneous quantitation of human nuclear, mitochondrial, and male Y-chromosome DNA |
| US20060111915A1 (en) * | 2004-11-23 | 2006-05-25 | Applera Corporation | Hypothesis generation |
| US8084260B2 (en) * | 2004-11-24 | 2011-12-27 | Applied Biosystems, Llc | Spectral calibration method and system for multiple instruments |
| US7892737B2 (en) * | 2005-06-30 | 2011-02-22 | Life Technologies Corporation | Compositions, kits and methods pertaining to stability modulation of PNA oligomer/nucleic acid complexes |
| GB0612342D0 (en) * | 2006-06-21 | 2006-08-02 | Glaxosmithkline Biolog Sa | Method |
| AU2007281535A1 (en) | 2006-08-01 | 2008-02-07 | Applied Biosystems, Llc. | Detection of analytes and nucleic acids |
| WO2010132756A2 (en) * | 2009-05-14 | 2010-11-18 | Streck, Inc. | Sample processing cassette, system, and method |
| US20140170653A1 (en) | 2011-04-15 | 2014-06-19 | Life Technologies Corporation | Chemical ligation |
| JP5920763B2 (ja) * | 2011-10-05 | 2016-05-18 | 国立大学法人名古屋大学 | 蛍光標識オリゴヌクレオチド誘導体及びその利用 |
| EP2660332B1 (de) * | 2012-05-01 | 2017-02-22 | F. Hoffmann-La Roche AG | Positives Steuerkonzept |
| US9540699B2 (en) | 2013-03-13 | 2017-01-10 | William Beaumont Hospital | Methods of diagnosing increased risk of developing MRSA |
| KR102207922B1 (ko) | 2014-03-06 | 2021-01-26 | 삼성전자주식회사 | 반코마이신 저항성 엔테로코커스 특이적인 프라이머 세트, 그를 포함하는 조성물 및 시료 중 반코마이신 저항성 엔테로코커스 속 미생물을 검출하는 방법 |
| GB2525024A (en) * | 2014-04-10 | 2015-10-14 | Vela Operations Pte Ltd | Universal controls for sequencing assays |
| WO2016059798A1 (ja) * | 2014-10-17 | 2016-04-21 | 国立大学法人 東京医科歯科大学 | 新規な陽性コントロール核酸を利用した、被検試料中の標的核酸の検出・定量法 |
| KR102287811B1 (ko) | 2014-10-31 | 2021-08-09 | 삼성전자주식회사 | 2개 표면을 결합시키는 방법 및 그에 의하여 제조된 구조체, 및 상기 구조체를 포함하는 미세유동 장치 |
| EP3458613B1 (de) * | 2016-05-18 | 2022-09-14 | Integrated Nano-Technologies, Inc. | Verfahren zur erkennung eines pcr-produkts |
| US11613777B2 (en) * | 2016-08-26 | 2023-03-28 | Life Technologies Corporation | Nucleic acid extraction and amplification controls and methods of use thereof |
| IT201900008781A1 (it) * | 2019-06-12 | 2020-12-12 | AB Analitica S R L | Metodo di amplificazione di acidi nucleici |
| JP7272202B2 (ja) | 2019-09-20 | 2023-05-12 | 株式会社島津製作所 | 標的核酸の検査方法および検査装置 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| JP2660661B2 (ja) * | 1994-05-11 | 1997-10-08 | 株式会社バイオセンサー研究所 | 遺伝子の定量方法 |
| US5801155A (en) * | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
| FR2740782B1 (fr) * | 1995-11-06 | 1998-10-16 | Microdiag | Procede et kit pour la quantification et la detection de micro-organismes |
| US5952202A (en) * | 1998-03-26 | 1999-09-14 | The Perkin Elmer Corporation | Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification |
| WO2000036152A1 (en) * | 1998-12-14 | 2000-06-22 | Li-Cor, Inc. | A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis |
-
2000
- 2000-08-24 US US09/645,959 patent/US6358679B1/en not_active Expired - Lifetime
-
2001
- 2001-08-23 DE DE60108897T patent/DE60108897T2/de not_active Expired - Lifetime
- 2001-08-23 JP JP2002522318A patent/JP2004507248A/ja active Pending
- 2001-08-23 AU AU85267/01A patent/AU8526701A/en not_active Abandoned
- 2001-08-23 WO PCT/US2001/026499 patent/WO2002016648A2/en not_active Ceased
- 2001-08-23 AT AT01964412T patent/ATE288964T1/de not_active IP Right Cessation
- 2001-08-23 EP EP01964412A patent/EP1339870B1/de not_active Expired - Lifetime
- 2001-08-23 CA CA002386913A patent/CA2386913A1/en not_active Abandoned
-
2002
- 2002-03-15 US US10/099,738 patent/US6890718B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US6358679B1 (en) | 2002-03-19 |
| EP1339870B1 (de) | 2005-02-09 |
| DE60108897D1 (de) | 2005-03-17 |
| US6890718B2 (en) | 2005-05-10 |
| AU8526701A (en) | 2002-03-04 |
| US20030027179A1 (en) | 2003-02-06 |
| DE60108897T2 (de) | 2006-03-02 |
| WO2002016648A3 (en) | 2003-06-19 |
| CA2386913A1 (en) | 2002-02-28 |
| WO2002016648A2 (en) | 2002-02-28 |
| JP2004507248A (ja) | 2004-03-11 |
| EP1339870A2 (de) | 2003-09-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |