ATE328115T1 - Multiplex genotypisierung unter verwendung von fluoreszenzmarkierten hybridisierungssonden - Google Patents

Multiplex genotypisierung unter verwendung von fluoreszenzmarkierten hybridisierungssonden

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Publication number
ATE328115T1
ATE328115T1 AT99950072T AT99950072T ATE328115T1 AT E328115 T1 ATE328115 T1 AT E328115T1 AT 99950072 T AT99950072 T AT 99950072T AT 99950072 T AT99950072 T AT 99950072T AT E328115 T1 ATE328115 T1 AT E328115T1
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AT
Austria
Prior art keywords
labled
fluorescence
hybridization probe
multiplex genotyping
hybridization probes
Prior art date
Application number
AT99950072T
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English (en)
Inventor
Carl T Wittwer
Philip S Bernard
Original Assignee
Univ Utah Res Found
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Publication of ATE328115T1 publication Critical patent/ATE328115T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Luminescent Compositions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AT99950072T 1998-09-30 1999-09-30 Multiplex genotypisierung unter verwendung von fluoreszenzmarkierten hybridisierungssonden ATE328115T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/164,023 US6140054A (en) 1998-09-30 1998-09-30 Multiplex genotyping using fluorescent hybridization probes

Publications (1)

Publication Number Publication Date
ATE328115T1 true ATE328115T1 (de) 2006-06-15

Family

ID=22592646

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99950072T ATE328115T1 (de) 1998-09-30 1999-09-30 Multiplex genotypisierung unter verwendung von fluoreszenzmarkierten hybridisierungssonden

Country Status (7)

Country Link
US (1) US6140054A (de)
EP (1) EP1117834B1 (de)
JP (1) JP3670967B2 (de)
AT (1) ATE328115T1 (de)
AU (1) AU6280599A (de)
DE (1) DE69931646T2 (de)
WO (1) WO2000018965A1 (de)

Families Citing this family (179)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE428801T1 (de) * 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
WO1999005574A1 (en) * 1997-07-25 1999-02-04 Affymetrix, Inc. Method and system for providing a probe array chip design database
US6826296B2 (en) 1997-07-25 2004-11-30 Affymetrix, Inc. Method and system for providing a probe array chip design database
US7068830B2 (en) * 1997-07-25 2006-06-27 Affymetrix, Inc. Method and system for providing a probe array chip design database
GB9803382D0 (en) * 1998-02-19 1998-04-15 Secr Defence Detection system
US6369893B1 (en) 1998-05-19 2002-04-09 Cepheid Multi-channel optical detection system
US20040199544A1 (en) * 2000-11-02 2004-10-07 Affymetrix, Inc. Method and apparatus for providing an expression data mining database
US6185561B1 (en) 1998-09-17 2001-02-06 Affymetrix, Inc. Method and apparatus for providing and expression data mining database
DE60045059D1 (de) * 1999-04-20 2010-11-18 Nat Inst Of Advanced Ind Scien Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US6472156B1 (en) * 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
WO2001055454A1 (en) * 2000-01-28 2001-08-02 Althea Technologies, Inc. Methods for analysis of gene expression
CA2403556A1 (en) * 2000-03-17 2001-09-27 Althea Technologies, Inc. A systematic approach to the analysis of gene function
EP1313880A2 (de) * 2000-05-30 2003-05-28 PE Corporation (NY) Methoden zur detektion gewünschter nukleinsäuren mittels kombinierter ligation und amplifizierung
EP1295941B1 (de) * 2000-06-27 2009-12-16 National Institute of Advanced Industrial Science and Technology Neue nukleinsäuresonden und verfahren zum testen von nukleinsäuren unter deren verwendung
GB0021286D0 (en) * 2000-08-30 2000-10-18 Gemini Genomics Ab Identification of drug metabolic capacity
EP1360324A2 (de) * 2000-08-31 2003-11-12 Mayo Foundation For Medical Education And Research Nachweis von varicella-zoster-virus
EP1197565A1 (de) * 2000-10-12 2002-04-17 Roche Diagnostics GmbH Neue Punktmutation im 3' Bereich des Prothrombingens, das mit gesteigerter Thrombosegefahr assoziiert ist
EP1207210B1 (de) * 2000-11-15 2005-10-12 Roche Diagnostics GmbH Verfahren zur Analyse von Wiederkehrenden PCR-Podukten, das auf der Analyse von DNS-Schmelzkurven basiert
ATE306562T1 (de) * 2000-11-15 2005-10-15 Hoffmann La Roche Verfahren zur analyse von wiederkehrenden pcr- podukten, das auf der analyse von dns- schmelzkurven basiert
US8900811B2 (en) * 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
ATE465272T1 (de) * 2001-01-31 2010-05-15 Mayo Foundation Nachweis von herpex-simplex-virus
US20020168641A1 (en) * 2001-03-09 2002-11-14 Bruce Mortensen Fluorescein-cyanine 5 as a fluorescence resonance energy transfer pair
WO2002083952A1 (en) * 2001-04-12 2002-10-24 Caliper Technologies Corp. Systems and methods for high throughput genetic analysis
AU2002339853A1 (en) * 2001-05-24 2002-12-03 Genospectra, Inc. Pairs of nucleic acid probes with interactive signaling moieties and nucleic acid probes with enhanced hybridization efficiency and specificity
US6825927B2 (en) * 2001-06-15 2004-11-30 Mj Research, Inc. Controller for a fluorometer
US6979541B1 (en) 2001-07-26 2005-12-27 University Of Utah Research Foundation Methods for identifying chromosomal aneuploidy
WO2003018837A2 (de) * 2001-08-24 2003-03-06 Adnagen Ag Verfahren und diagnosekit zur molekularen diagnostik pharmakologisch relevanter gene
US6593091B2 (en) 2001-09-24 2003-07-15 Beckman Coulter, Inc. Oligonucleotide probes for detecting nucleic acids through changes in flourescence resonance energy transfer
US20040029105A1 (en) * 2001-11-02 2004-02-12 Smith Thomas F. Detection of variola virus
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
CA2476481C (en) * 2002-02-21 2016-01-26 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
WO2003073911A2 (en) * 2002-02-28 2003-09-12 Georgetown University Method and composition for detection and treatment of breast cancer
US6977162B2 (en) 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
DE10215238C1 (de) * 2002-04-06 2003-08-14 Cytonet Gmbh & Co Kg Nachweis von Mykobakterien in klinischem Material
WO2003093502A2 (de) * 2002-04-30 2003-11-13 Gnothis Holding Sa Multiplex-nachweis von nukleinsäure-polymorphismen
US7442506B2 (en) 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US7785776B2 (en) 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
US20030215814A1 (en) * 2002-05-17 2003-11-20 Cockerill Franklin R. Detection of Shiga toxin- or Shiga-like toxin-producing organisms
WO2003097828A1 (fr) * 2002-05-21 2003-11-27 Adgene Co., Ltd. Procede d'identification d'acides nucleiques
US20030219754A1 (en) * 2002-05-23 2003-11-27 Oleksy Jerome E. Fluorescence polarization detection of nucleic acids
US20030220844A1 (en) * 2002-05-24 2003-11-27 Marnellos Georgios E. Method and system for purchasing genetic data
JP4457001B2 (ja) * 2002-05-31 2010-04-28 セクレタリー・デパートメント・オブ・アトミック・エナジー ドナー/アクセプター部分が相補鎖上となる標的核酸検出のmet/fretに基づく方法
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
AU2003244135A1 (en) * 2002-06-21 2004-01-06 Hinode Co, . Ltd. Device for removing raindrops on umrella and system for promoting installation of the device
EP1389638A1 (de) * 2002-08-06 2004-02-18 Roche Diagnostics GmbH Verbesserte Proben für FRET
US7074598B2 (en) * 2002-09-25 2006-07-11 Mayo Foundation For Medical Education And Research Detection of vancomycin-resistant enterococcus spp.
US20040235005A1 (en) * 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
EP1558757A4 (de) * 2002-11-01 2006-06-07 Megabase Res Products Gasstrahl-verfahren und gerät für dna-hochgeschwindigkeitsamplifikation
WO2004042404A1 (en) * 2002-11-07 2004-05-21 Erasmus Universiteit Rotterdam Fret probes and methods for detecting interacting molecules
EP2112229A3 (de) 2002-11-25 2009-12-02 Sequenom, Inc. Verfahren zum Identifizieren des Brustkrebsrisikos und zugehörige Behandlungen
EP3000899A1 (de) 2002-12-04 2016-03-30 Applied Biosystems, LLC Multiplexverstärkung von polynucleotiden
WO2005010201A2 (en) * 2003-01-29 2005-02-03 Naxcor, Inc. Methods and compositions for detecting nucleic acid sequences
GB0303497D0 (en) * 2003-02-15 2003-03-19 Univ Liverpool Immuno PCR method
WO2004074447A2 (en) * 2003-02-18 2004-09-02 Applera Corporation Compositions and methods for multiplex analysis of polynucleotides
US20060192960A1 (en) * 2003-03-24 2006-08-31 Rencs Erik V Polarization detection
WO2004087950A2 (en) * 2003-04-04 2004-10-14 Roche Diagnostics Gmbh Improved system for multi color real time pcr
JP4336877B2 (ja) * 2003-04-18 2009-09-30 アークレイ株式会社 β3アドレナリン受容体変異遺伝子の検出法ならびにそのための核酸プローブおよびキット
US7148043B2 (en) 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
US20040229211A1 (en) * 2003-05-13 2004-11-18 Yeung Wah Hin Alex Sensitive diagnostic testing methodology using multiplex real time PCR with one dye (MOD) and its use as in severe acute respiratory syndrome (SARS)
US20050239089A1 (en) * 2003-06-06 2005-10-27 Johnson Martin D Mobility cassettes
JP4571786B2 (ja) * 2003-06-10 2010-10-27 則夫 清水 標的核酸の検出法
EP1493822A1 (de) * 2003-07-02 2005-01-05 Labor Becker, Olgemoeller & Kollegen GbR Detektionsverfahren für Nucleinsäuren mit interner Amplifikationskontrolle
US20060024690A1 (en) * 2003-09-19 2006-02-02 Kao H P Normalization of data using controls
WO2005029041A2 (en) * 2003-09-19 2005-03-31 Applera Corporation High density sequence detection methods and apparatus
US7427475B2 (en) * 2003-11-18 2008-09-23 Mayo Foundation For Medical Education And Research Detection of group B streptococcus
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
FR2866349B1 (fr) * 2004-02-12 2008-05-16 Biomerieux Sa Procede de diagnostic/pronostic d'une thrombose
EP1725587A2 (de) * 2004-03-19 2006-11-29 U.S. Genomics, Inc. Zusammensetzungen und verfahren zum nachweis von einzelnen molekülen
US7473529B1 (en) * 2004-03-31 2009-01-06 Iowa State University Research Foundation, Inc. System and method for detecting cells or components thereof
WO2005113741A1 (en) * 2004-05-12 2005-12-01 Board Of Regents Of University Of Nebraska Vortex tube thermocycler
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
EP2290073A3 (de) * 2004-05-28 2011-08-31 Asuragen, Inc. Verfahren und Zusammensetzungen mit MicroRNA
US20060003337A1 (en) * 2004-06-30 2006-01-05 John Brandis Detection of small RNAS
WO2006022628A1 (en) * 2004-07-22 2006-03-02 Sequenom, Inc. Methods for identifying risk of type ii diabetes and treatments thereof
EP2093297A3 (de) 2004-09-07 2009-11-18 Telethon Institute for Child Health Research Wirkstoffe zur Behandlung oder Vorbeugung einer allergischen Störung
ES2668467T3 (es) * 2004-10-18 2018-05-18 Brandeis University Métodos de amplificación LATE y secuenciación de un ácido nucleico
US20060194222A1 (en) * 2004-10-20 2006-08-31 Stratagene California Triplex probe compositions and methods for polynucleotide detection
CA2857878A1 (en) 2004-11-12 2006-12-28 Asuragen, Inc. Methods and compositions involving mirna and mirna inhibitor molecules
US20060188871A1 (en) * 2005-02-24 2006-08-24 Jackwood Daral J Detection of very virulent infectious bursal disease virus
WO2006095941A1 (en) 2005-03-05 2006-09-14 Seegene, Inc. Processes using dual specificity oligonucleotide and dual specificity oligonucleotide
CA2842402C (en) 2005-03-10 2016-02-23 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
US7919242B2 (en) * 2005-06-30 2011-04-05 Roche Molecular Systems, Inc. Light emission modifiers and their uses in nucleic acid detection, amplification and analysis
EP1929049B1 (de) 2005-07-25 2013-04-10 Alere San Diego, Inc. Verfahren zur multiplexierung einer rekombinase-polymerase-verstärkung
US7785786B2 (en) * 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
RU2304170C1 (ru) * 2006-02-08 2007-08-10 Общество с ограниченной ответственностью "Университетская медицина" Способ одновременного днк-тестирования на наличие полиморфизмов н63d и c282y в гене hfe, связанных с наследственным гемохроматозом
US8778637B2 (en) * 2006-03-28 2014-07-15 Canon U.S. Life Sciences, Inc. Method and apparatus for applying continuous flow and uniform temperature to generate thermal melting curves in a microfluidic device
FR2899689B1 (fr) * 2006-04-10 2016-05-20 Cis Bio Int Procede de suppression d'un signal fret, agents suppresseurs d'un signal de fret et utilisation dans un procede de multiplexage biologiques
US20070238093A1 (en) * 2006-04-11 2007-10-11 Espy Mark J Detection of influenza A virus
US20070238095A1 (en) * 2006-04-11 2007-10-11 Mayo Foundation For Medical Education And Research , A Minnesota Corporation Detection of Influenza A Virus
EP1845166A1 (de) * 2006-04-13 2007-10-17 BioSpring GmbH Neue Nukleinsäuresonden mit kovalent gebundenen, interkalierenden Fluoreszenzfarbstoffen zur Hybridisierung an Nukleinsäureproben
JP2009535053A (ja) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック, インコーポレイテッド レコンビナーゼポリメラーゼ増幅
EP3260556B1 (de) 2006-05-31 2019-07-31 Sequenom, Inc. Verfahren zur nukleinsäureextraktion aus einer probe
WO2007140599A1 (en) 2006-06-05 2007-12-13 Cancer Care Ontario Assessment of risk for colorectal cancer
CA2668457C (en) * 2006-11-02 2016-10-04 Veridex, Llc Imaging of activated vascular endothelium using immunomagnetic mri contrast agents
US9114398B2 (en) 2006-11-29 2015-08-25 Canon U.S. Life Sciences, Inc. Device and method for digital multiplex PCR assays
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
WO2008073919A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-20 regulated genes and pathways as targets for therapeutic intervention
CN102851369B (zh) 2006-12-13 2015-01-21 卢米耐克斯公司 用于实时pcr多重分析的系统和方法
CA2677517C (en) 2007-02-08 2015-11-03 Sequenom, Inc. Nucleic acid-based tests for rhd typing, gender determination and nucleic acid quantification
US20110189663A1 (en) 2007-03-05 2011-08-04 Cancer Care Ontario Assessment of risk for colorectal cancer
AU2008230813B2 (en) 2007-03-26 2014-01-30 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
WO2008128198A2 (en) 2007-04-12 2008-10-23 Usc Stevens - University Of Southern California Dna methylation analysis by digital bisulfite genomic sequencing and digital methylight
US20080274458A1 (en) * 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
EP2014774A1 (de) 2007-07-11 2009-01-14 Pathofinder B.V. Assay für den gleichzeitigen Nachweis mehrerer Nukleinsäuresequenzen in einer Probe
US9512470B2 (en) 2007-07-11 2016-12-06 Pathofinder Holding B.V. Method for the simultaneous detection of multiple nucleic acid sequences in a sample
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
EP2198050A1 (de) 2007-09-14 2010-06-23 Asuragen, INC. Mikrornas mit unterschiedlicher expression bei zervikalkarzinom und verwendungen davon
WO2009070805A2 (en) 2007-12-01 2009-06-04 Asuragen, Inc. Mir-124 regulated genes and pathways as targets for therapeutic intervention
EP2252702B1 (de) 2008-02-08 2014-01-29 Mayo Foundation for Medical Education and Research Nachweis von clostridium difficile
WO2009103003A2 (en) * 2008-02-15 2009-08-20 Bio-Rad Laboratories, Inc. Scanning fluorescent reader with diffuser system
JP2009201444A (ja) * 2008-02-29 2009-09-10 Hitachi High-Technologies Corp 核酸分析装置
WO2009114543A2 (en) 2008-03-11 2009-09-17 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US8206926B2 (en) 2008-03-26 2012-06-26 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
GB0909333D0 (en) 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
US9234232B2 (en) 2008-07-31 2016-01-12 Oxitec Limited Multiplex amplification and detection
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US9540686B2 (en) * 2008-09-18 2017-01-10 Canon U.S. Life Sciences, Inc. Systems and methods for the amplification of DNA
JP2012511328A (ja) * 2008-12-10 2012-05-24 スミスズ ディテクション インコーポレイティド 温度依存ハイブリダイゼーションを使用した核酸配列の同定及び区別
JP5279481B2 (ja) * 2008-12-25 2013-09-04 株式会社日立ハイテクノロジーズ 核酸分析装置
CA2751888C (en) 2009-02-11 2022-06-14 Caris Mpi, Inc. Molecular profiling of tumors
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
EP2414545B1 (de) 2009-04-03 2017-01-11 Sequenom, Inc. Zusammensetzungen und verfahren für die herstellung von nukleinsäuren
BRPI1006595A2 (pt) * 2009-04-15 2017-09-26 Relia Diagnostic Systems Inc tira de teste e método para detectar a presença de um análito em uma amostra
US9469867B2 (en) 2009-05-20 2016-10-18 Alere San Diego, Inc. DNA glycosylase/lyase and AP endonuclease substrates
WO2010135692A2 (en) 2009-05-22 2010-11-25 Asuragen, Inc. Mirna biomarkers of prostate disease
US8691504B2 (en) 2009-05-26 2014-04-08 Xiamen University Method for detecting variations in nucleic acid sequences
WO2011022589A2 (en) * 2009-08-20 2011-02-24 Mayo Foundation For Medical Education And Research Detection of enterovirus
CN102725632A (zh) 2009-08-28 2012-10-10 奥斯瑞根公司 肺病的miRNA生物标志物
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
US9353407B2 (en) 2009-10-21 2016-05-31 Brandeis University Methods, kits and reaction mixtures for analyzing single-stranded nucleic acid sequences
CA2779223A1 (en) 2009-10-27 2011-05-12 Caris Mpi, Inc. Molecular profiling for personalized medicine
EP2516675A1 (de) * 2009-12-21 2012-10-31 Becton, Dickinson and Company Verfahren zur identifizierung von wirkstoffresistenten mykobakterien
DK2516680T3 (en) 2009-12-22 2016-05-02 Sequenom Inc Method and kits to identify aneuploidy
EP3460458B1 (de) 2010-02-19 2021-08-11 Pacific Biosciences of California, Inc. Verfahren zur nukleinsäuresequenzierung
JP2013528379A (ja) 2010-05-21 2013-07-11 オームクス コーポレーション Psa酵素活性アッセイによる癌の検出
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
EP2601307A4 (de) 2010-08-06 2014-01-01 Capitalbio Corp Microarray-test mit partikeln zur analyse von molekularen interaktionen
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
US9310375B2 (en) 2010-10-27 2016-04-12 Capitalbio Corporation Luminophore-labeled molecules coupled with particles for microarray-based assays
BR112013010585B1 (pt) 2010-10-29 2020-12-01 Asuragen, Inc. método de caracterização de um locus de fmr1 ou um locus de frm2 em uma amostra de dna e método de análise de um locus fmr1 ou um locus fmr2 de uma amostra de dna humana
AU2012222178B2 (en) 2011-02-24 2014-12-18 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
EP2702168B1 (de) 2011-04-29 2018-01-17 Sequenom, Inc. Quantifizierung einer minderheitsvariante einer nukleinsäure
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
CN103998921B (zh) * 2011-10-14 2016-04-20 贝克顿·迪金森公司 方波热循环
EP2773892B1 (de) 2011-11-04 2020-10-07 Handylab, Inc. Vorrichtung zur vorbereitung von polynukleotidproben
US9605313B2 (en) 2012-03-02 2017-03-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2872648B1 (de) 2012-07-13 2019-09-04 Sequenom, Inc. Verfahren und zusammensetzungen zur methylierungsbasierten anreicherung fetaler nukleinsäure aus einer mütterlichen probe für nicht-invasive pränatale diagnosen
US9850549B2 (en) 2012-10-09 2017-12-26 University Of Utah Research Foundation Monitoring temperature with fluorescence
US9896728B2 (en) 2013-01-29 2018-02-20 Arcticrx Ltd. Method for determining a therapeutic approach for the treatment of age-related macular degeneration (AMD)
WO2014168711A1 (en) 2013-03-13 2014-10-16 Sequenom, Inc. Primers for dna methylation analysis
WO2015042493A2 (en) 2013-09-20 2015-03-26 Ohmx Corporation Psa enzymatic activity: a new biomarker for assessing prostate cancer aggressiveness
CN122038564A (zh) 2014-01-05 2026-05-15 G·索兹 使用mirna比率确定肺癌
US10619210B2 (en) 2014-02-07 2020-04-14 The Johns Hopkins University Predicting response to epigenetic drug therapy
US11365447B2 (en) 2014-03-13 2022-06-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
ES2832748T3 (es) 2014-08-19 2021-06-11 Hoffmann La Roche Procedimientos y composiciones para la detección de ácidos nucleicos
US20160097082A1 (en) 2014-09-23 2016-04-07 Ohmx Corporation Prostate specific antigen proteolytic activity for clinical use
WO2016094330A2 (en) 2014-12-08 2016-06-16 20/20 Genesystems, Inc Methods and machine learning systems for predicting the liklihood or risk of having cancer
CN107532213B (zh) * 2015-04-23 2021-05-11 病理取景器控股有限责任公司 用于同时检测样品中多个核酸序列的方法
EP3292214A1 (de) 2015-05-04 2018-03-14 Academisch Medisch Centrum Verfahren zur quantifizierung von mirnas mittels normalisierung
WO2017015543A1 (en) 2015-07-23 2017-01-26 Asuragen, Inc. Methods, compositions, kits, and uses for analysis of nucleic acids comprising repeating a/t-rich segments
WO2018045162A1 (en) 2016-09-01 2018-03-08 Biogen Ma Inc. Biomarkers predictive of primary progressive multiple sclerosis and uses thereof
JP6786618B2 (ja) * 2016-10-17 2020-11-18 オリンパス株式会社 標的核酸分子の検出方法
CN107058478B (zh) * 2016-12-08 2022-02-11 广东药科大学 一种检测MTHFR基因rs1801131多态位点基因型的方法及试剂盒
GB201704401D0 (en) 2017-03-20 2017-05-03 Epistem Ltd Use of anticoagulants
RU2644262C1 (ru) * 2017-04-17 2018-02-08 Александр Юрьевич Криворучко Способ детекции множества последовательностей нуклеотидов в одном образце методом кодирования ампликонов при проведении полимеразной цепной реакции
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
WO2020113237A1 (en) 2018-11-30 2020-06-04 Caris Mpi, Inc. Next-generation molecular profiling
EP3719144A1 (de) 2019-04-05 2020-10-07 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Mir-151a-3p als universelle endogene steuerung zur exosomenladungsnormalisierung
US20220267857A1 (en) 2019-07-19 2022-08-25 Fundación Para La Investigación Biomédikca Del Hospital Universitario La Paz (Fibhulp) Method for determining the response to treatment of a patient affected by non-small cell lung carcinoma (nsclc)
KR20260043144A (ko) 2019-12-02 2026-03-31 캐리스 엠피아이, 아이엔씨. 범-암 백금 반응 예측기
CN115414703B (zh) * 2022-11-03 2023-01-17 百力格生物科技(上海)股份有限公司 染料修饰后的核酸探针纯化方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4234086A1 (de) * 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
US5403707A (en) * 1993-05-14 1995-04-04 Eastman Kodak Company Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures
US5591578A (en) * 1993-12-10 1997-01-07 California Institute Of Technology Nucleic acid mediated electron transfer
US5475098A (en) * 1994-06-14 1995-12-12 The United States Of America As Represented By The Department Of Health And Human Services Distinctive DNA sequence of E. coli 0157:H7 and its use for the rapid, sensitive and specific detection of 0157:H7 and other enterohemorrhagic E. coli
IL124967A (en) * 1995-12-18 2000-07-26 Univ Washington Method for nucleic acid analysis using fluorescence resonance energy transfer
ATE428801T1 (de) * 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
PT2298931E (pt) * 1996-06-04 2013-11-11 Univ Utah Res Found Aparelho para executar uma pcr e monitorização fluorescente contínua da reacção de pcr
US5691145A (en) * 1996-08-27 1997-11-25 Becton, Dickinson And Company Detection of nucleic acids using G-quartets
GB2333597B (en) * 1996-10-29 2001-04-18 Univ Nebraska At Lincoln Method for detecting point mutations in dna utilizing fluorescence energy transfer
GB9707996D0 (en) * 1997-04-21 1997-06-11 Univ Cambridge Tech Screening

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