ATE294870T1 - Methode zur herstellung der proteinase nla aus tabak - Google Patents
Methode zur herstellung der proteinase nla aus tabakInfo
- Publication number
- ATE294870T1 ATE294870T1 AT94907952T AT94907952T ATE294870T1 AT E294870 T1 ATE294870 T1 AT E294870T1 AT 94907952 T AT94907952 T AT 94907952T AT 94907952 T AT94907952 T AT 94907952T AT E294870 T1 ATE294870 T1 AT E294870T1
- Authority
- AT
- Austria
- Prior art keywords
- plant virus
- proteinase
- proteins
- nla
- tobacco
- Prior art date
Links
- 102000035195 Peptidases Human genes 0.000 title abstract 4
- 108091005804 Peptidases Proteins 0.000 title abstract 4
- 235000019833 protease Nutrition 0.000 title abstract 4
- 241000208125 Nicotiana Species 0.000 title 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 241000700605 Viruses Species 0.000 abstract 3
- 102000037865 fusion proteins Human genes 0.000 abstract 2
- 108020001507 fusion proteins Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 102000014914 Carrier Proteins Human genes 0.000 abstract 1
- 108010078791 Carrier Proteins Proteins 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000017854 proteolysis Effects 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/021,603 US5532142A (en) | 1993-02-12 | 1993-02-12 | Method of isolation and purification of fusion polypeptides |
| PCT/US1994/001176 WO1994018331A2 (en) | 1993-02-12 | 1994-01-31 | Fusion proteins including a cleavage site recognized by a plant virus protease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE294870T1 true ATE294870T1 (de) | 2005-05-15 |
Family
ID=21805141
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT94907952T ATE294870T1 (de) | 1993-02-12 | 1994-01-31 | Methode zur herstellung der proteinase nla aus tabak |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US5532142A (de) |
| EP (1) | EP0682709B1 (de) |
| AT (1) | ATE294870T1 (de) |
| AU (1) | AU6132294A (de) |
| DE (1) | DE69434358D1 (de) |
| WO (1) | WO1994018331A2 (de) |
Families Citing this family (77)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5821088A (en) * | 1990-05-11 | 1998-10-13 | Siga Pharmaceuticals, Inc. | Use of gram-positive bacteria to express recombinant proteins |
| US5593877A (en) * | 1993-03-11 | 1997-01-14 | The Rockefeller University | Nucleic acid and recombinant production of vespid venom hyaluronidase |
| US5955647A (en) | 1994-02-03 | 1999-09-21 | The Scripps Research Institute | Method for using tobacco mosaic virus to overproduce peptides and proteins |
| JP3637087B2 (ja) * | 1994-11-28 | 2005-04-06 | 和男 鈴木 | 新規な白血球活性化因子 |
| US5795587A (en) * | 1995-01-23 | 1998-08-18 | University Of Pittsburgh | Stable lipid-comprising drug delivery complexes and methods for their production |
| CA2218601A1 (en) * | 1995-03-24 | 1996-10-03 | Ophidian Pharmaceuticals, Inc. | Treatment for verotoxin-producing escherichia coli |
| JPH11505421A (ja) | 1995-05-11 | 1999-05-21 | ヒューマン ジノーム サイエンシーズ，インコーポレイテッド | ヒトウリジンジホスフェートガラクトース−４−エピメラーゼ |
| US20030069173A1 (en) * | 1998-03-16 | 2003-04-10 | Life Technologies, Inc. | Peptide-enhanced transfections |
| US6051429A (en) | 1995-06-07 | 2000-04-18 | Life Technologies, Inc. | Peptide-enhanced cationic lipid transfections |
| AT404838B (de) * | 1995-11-24 | 1999-03-25 | Immuno Ag | Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen |
| US5935824A (en) * | 1996-01-31 | 1999-08-10 | Technologene, Inc. | Protein expression system |
| JP2000510332A (ja) * | 1996-04-19 | 2000-08-15 | ヘンリー エム．ジャクソン ファンデーション フォー ザ アドバンスメント オブ ミリタリー メディシン | インチミンを単独で、または１以上の他の抗原との融合タンパク質として発現する宿主生物の投与による、免疫応答を刺激する方法 |
| US20030040038A1 (en) * | 1997-08-22 | 2003-02-27 | Steven F. Dowdy | Inducible regulatory system and use thereof |
| US6960457B1 (en) * | 1997-09-04 | 2005-11-01 | Stanford University | Reversible immobilization of arginine-tagged moieties on a silicate surface |
| CA2314267A1 (en) | 1997-12-10 | 1999-06-17 | Washington University | Anti-pathogen system and methods of use thereof |
| ATE393912T1 (de) | 1998-02-04 | 2008-05-15 | Invitrogen Corp | Microarrays und ihre verwendungen |
| US6506595B2 (en) * | 1998-03-31 | 2003-01-14 | Itoham Foods Inc. | DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs |
| US6406921B1 (en) * | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
| US20030138973A1 (en) * | 1998-07-14 | 2003-07-24 | Peter Wagner | Microdevices for screening biomolecules |
| US6897073B2 (en) * | 1998-07-14 | 2005-05-24 | Zyomyx, Inc. | Non-specific binding resistant protein arrays and methods for making the same |
| US6576478B1 (en) * | 1998-07-14 | 2003-06-10 | Zyomyx, Inc. | Microdevices for high-throughput screening of biomolecules |
| US6913749B2 (en) * | 1998-11-02 | 2005-07-05 | Resistentia Pharmaceuticals Ab | Immunogenic polypeptides for inducing anti-self IgE responses |
| CA2350882C (en) | 1998-11-12 | 2011-02-22 | Life Technologies Corporation | Transfection reagents |
| US7262005B1 (en) | 2000-02-04 | 2007-08-28 | Aurora Biosciences Corporation | Methods of protein destabilization and uses thereof |
| CA2408291C (en) * | 2000-05-04 | 2014-07-15 | Yale University | High density protein arrays for screening of protein activity |
| AU2001268273A1 (en) | 2000-06-15 | 2001-12-24 | Yale University | Mutant proteinase with reduced self-cleavage activity and method of purification |
| JP2006513692A (ja) * | 2002-04-22 | 2006-04-27 | ジェネンコー・インターナショナル・インク | 様々なレベルの遺伝子発現を生じる修飾プロモーターを作成する方法 |
| US7825227B2 (en) * | 2002-05-09 | 2010-11-02 | Prolexys Pharmaceuticals, Inc. | Method for purification of a protein complex and identification of its components |
| CA2513072A1 (en) * | 2003-01-09 | 2004-07-29 | Invitrogen Corporation | Cellular delivery and activation polypeptide-nucleic acid complexes |
| EP2228379A1 (de) | 2003-04-25 | 2010-09-15 | North Carolina State University | Für Zelloberflächenproteinhomologe codierende Nukleinsäuresequenzen aus Lactobacillus acidophilus |
| WO2005001057A2 (en) | 2003-06-23 | 2005-01-06 | North Carolina State University | Lactobacillus acidophilus nucleic acids encoding fructo-oligosaccharide utilization compounds and uses thereof |
| BRPI0507169A (pt) * | 2004-02-02 | 2007-06-26 | Ambrx Inc | polipeptìdeos do hormÈnio de crescimento humano modificados e seu usos |
| US7459289B2 (en) | 2004-03-08 | 2008-12-02 | North Carolina State University | Lactobacillus acidophilus nucleic acid sequences encoding carbohydrate utilization-related proteins and uses therefor |
| EP1771205B1 (de) * | 2004-06-18 | 2016-10-26 | Ambrx, Inc. | Neuartige antigen-bindende polypeptide und ihre verwendungen |
| JP2008507280A (ja) * | 2004-07-21 | 2008-03-13 | アンブレツクス・インコーポレイテツド | 非天然コードアミノ酸を用いた生合成ポリペプチド |
| BRPI0519170A8 (pt) * | 2004-12-22 | 2018-05-08 | Ambrx Inc | formulações de hormônio de crescimento humano que compreendem um aminoácido não naturalmente codificado |
| JP4990792B2 (ja) * | 2004-12-22 | 2012-08-01 | アンブレツクス・インコーポレイテツド | アミノアシル−ｔＲＮＡシンテターゼの組成物およびこの使用 |
| CA2594557C (en) | 2004-12-22 | 2016-04-26 | Ambrx, Inc. | Modified human growth hormone |
| JP2008525032A (ja) | 2004-12-22 | 2008-07-17 | アンブレツクス・インコーポレイテツド | 組換えヒト成長ホルモンを発現及び精製するための方法 |
| US8137929B2 (en) * | 2005-04-15 | 2012-03-20 | Novo Nordisk Health Care Ag | Basic protein purification tags from thermophilic bacteria |
| JP2008537885A (ja) | 2005-04-15 | 2008-10-02 | ノース・キャロライナ・ステイト・ユニヴァーシティ | 細菌の付着およびストレス耐性を修飾する方法および組成物 |
| KR20080026135A (ko) * | 2005-06-03 | 2008-03-24 | 암브룩스, 인코포레이티드 | 개선된 인간 인터페론 분자 및 이의 용도 |
| CN101595228A (zh) | 2005-07-21 | 2009-12-02 | 艾博特公司 | 包括sorf构建体的多基因表达和使用多蛋白、前体蛋白和蛋白酶解的方法 |
| WO2007021297A1 (en) * | 2005-08-18 | 2007-02-22 | Ambrx, Inc. | COMPOSITIONS OF tRNA AND USES THEREOF |
| HRP20110404T1 (hr) * | 2005-11-08 | 2011-08-31 | Ambrx | Ubrzivači za modifikaciju ne-prirodnih aminokiselina i polipeptida ne-prirodnih aminokiselina |
| EP1951890A4 (de) * | 2005-11-16 | 2009-06-24 | Ambrx Inc | Verfahren und zusammensetzungen mit nichtnatürlichen aminosäuren |
| KR101423898B1 (ko) * | 2005-12-14 | 2014-07-28 | 암브룩스, 인코포레이티드 | 비-천연 아미노산 및 폴리펩티드를 함유하는 조성물, 비-천연 아미노산 및 폴리펩티드를 포함하는 방법, 및 비-천연 아미노산 및 폴리펩티드의 용도 |
| AU2007292892B9 (en) * | 2006-09-08 | 2013-02-28 | Ambrx, Inc. | Hybrid suppressor tRNA for vertebrate cells |
| KR20090060294A (ko) * | 2006-09-08 | 2009-06-11 | 암브룩스, 인코포레이티드 | 변형된 인간 혈장 폴리펩티드 또는 Ｆｃ 스캐폴드 및 그의 용도 |
| CN101511856B (zh) * | 2006-09-08 | 2016-01-20 | Ambrx公司 | 脊椎动物细胞中抑制因子trna的转录 |
| CN101563358A (zh) * | 2006-10-13 | 2009-10-21 | 诺沃-诺迪斯克保健股份有限公司 | 融合于碱性蛋白标签的加工酶 |
| CA2682147C (en) | 2007-03-30 | 2017-08-08 | Ambrx, Inc. | Modified fgf-21 polypeptides and their uses |
| CN101675071B (zh) * | 2007-05-02 | 2014-06-18 | Ambrx公司 | 经修饰干扰素β多肽和其用途 |
| CA2703830A1 (en) * | 2007-11-20 | 2009-05-28 | Ambrx, Inc. | Modified insulin polypeptides and their uses |
| SG188143A1 (en) | 2008-02-08 | 2013-03-28 | Ambrx Inc | Modified leptin polypeptides and their uses |
| EP2318029B1 (de) * | 2008-07-23 | 2017-11-01 | Ambrx, Inc. | Modifizierte bovine g-csf-polypeptide und ihre verwendung |
| US8278418B2 (en) | 2008-09-26 | 2012-10-02 | Ambrx, Inc. | Modified animal erythropoietin polypeptides and their uses |
| PE20110480A1 (es) | 2008-09-26 | 2011-07-01 | Ambrx Inc | Microorganismos y vacunas dependientes de replicacion de aminoacidos no naturales |
| BRPI1014089A2 (pt) | 2009-04-02 | 2016-04-19 | Roche Glycart Ag | anticorpos multiespecíficos que compreendem anticorpos de comprimento completo e fragmentos fab de cadeia simples |
| CN102712696A (zh) * | 2009-09-16 | 2012-10-03 | 弗·哈夫曼-拉罗切有限公司 | 包含卷曲螺旋和/或系链的蛋白质复合体及其用途 |
| JP2013509188A (ja) * | 2009-10-30 | 2013-03-14 | アボット・ラボラトリーズ | Ｓｏｒｆ構築物および複数の遺伝子発現 |
| TW201138821A (en) | 2010-03-26 | 2011-11-16 | Roche Glycart Ag | Bispecific antibodies |
| US9567386B2 (en) | 2010-08-17 | 2017-02-14 | Ambrx, Inc. | Therapeutic uses of modified relaxin polypeptides |
| PE20140160A1 (es) | 2010-08-17 | 2014-02-08 | Ambrx Inc | Polipeptidos de relaxina modificados y sus usos |
| CN103068846B9 (zh) | 2010-08-24 | 2016-09-28 | 弗·哈夫曼-拉罗切有限公司 | 包含二硫键稳定性Fv片段的双特异性抗体 |
| TWI480288B (zh) | 2010-09-23 | 2015-04-11 | Lilly Co Eli | 牛顆粒細胞群落刺激因子及其變體之調配物 |
| EP2655413B1 (de) | 2010-12-23 | 2019-01-16 | F.Hoffmann-La Roche Ag | Polypeptid-polynukleotid-komplex und verwendung bei der gezielten effektorrest-bereitstellung |
| MX2014009565A (es) | 2012-02-10 | 2014-11-10 | Genentech Inc | Anticuerpos monocatenarios y otros heteromultimeros. |
| ES2597228T3 (es) | 2012-06-27 | 2017-01-17 | F. Hoffmann-La Roche Ag | Procedimiento para la selección y la producción de entidades de objetivación, como dianas, altamente selectivas y multiespecíficas, personalizadas, las cuales contienen por lo menos dos entidades de unión diferentes, y utilización de éstas |
| KR20150030744A (ko) | 2012-06-27 | 2015-03-20 | 에프. 호프만-라 로슈 아게 | 표적에 특이적으로 결합하는 하나 이상의 결합 단위를 포함하는 항체 Ｆｃ－영역 접합체의 제조 방법 및 그의 용도 |
| KR101536963B1 (ko) * | 2013-09-17 | 2015-07-16 | 재단법인 전주생물소재연구소 | 식물에서 tev 프로테아제를 대량생산하는 방법 및 상기 방법에 의해 제조된 tev 프로테아제 |
| US10195280B2 (en) | 2014-07-15 | 2019-02-05 | Life Technologies Corporation | Compositions and methods for efficient delivery of molecules to cells |
| MX2017004947A (es) | 2014-10-24 | 2017-06-29 | Bristol Myers Squibb Co | Polipeptidos del factor de crecimiento de fibroblastos 2 (fgf-21) modificados y usos de los mismos. |
| WO2016087416A1 (en) | 2014-12-03 | 2016-06-09 | F. Hoffmann-La Roche Ag | Multispecific antibodies |
| SG10201912034UA (en) | 2017-02-08 | 2020-02-27 | Bristol Myers Squibb Co | Modified relaxin polypeptides comprising a pharmacokinetic enhancer and uses thereof |
| EP4486858A1 (de) | 2022-03-01 | 2025-01-08 | Danisco US Inc. | Enzyme und enzymzusammensetzungen zur reinigung |
| CN116144689A (zh) * | 2023-03-06 | 2023-05-23 | 优睿赛思(武汉)生物科技有限公司 | 重组猫血清淀粉样蛋白a的表达载体、宿主菌及其制备方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5221619A (en) * | 1977-11-08 | 1993-06-22 | Genentech, Inc. | Method and means for microbial polypeptide expression |
| SE8300693L (sv) * | 1983-02-09 | 1984-08-10 | Sven Lofdahl | Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta |
| CA1340522C (en) * | 1987-03-10 | 1999-05-04 | Heinz Dobeli | Fusion proteins containing neighbouring histidines for improved purification |
| NZ224663A (en) * | 1987-05-28 | 1990-11-27 | Amrad Corp Ltd | Fusion proteins containing glutathione-s-transferase and expression of foreign polypeptides using glutathione-s-transferase encoding vectors |
| US5179007A (en) * | 1989-07-07 | 1993-01-12 | The Texas A & M University System | Method and vector for the purification of foreign proteins |
| US5162601A (en) * | 1989-11-22 | 1992-11-10 | The Upjohn Company | Plant potyvirus expression vector with a gene for protease |
| GB8927008D0 (en) * | 1989-11-29 | 1990-01-17 | Ciba Geigy | Novel process for the production of unfused protein in e.coli |
-
1993
- 1993-02-12 US US08/021,603 patent/US5532142A/en not_active Expired - Lifetime
-
1994
- 1994-01-31 WO PCT/US1994/001176 patent/WO1994018331A2/en not_active Ceased
- 1994-01-31 AT AT94907952T patent/ATE294870T1/de not_active IP Right Cessation
- 1994-01-31 DE DE69434358T patent/DE69434358D1/de not_active Expired - Lifetime
- 1994-01-31 EP EP94907952A patent/EP0682709B1/de not_active Expired - Lifetime
- 1994-01-31 AU AU61322/94A patent/AU6132294A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO1994018331A2 (en) | 1994-08-18 |
| US5532142A (en) | 1996-07-02 |
| DE69434358D1 (de) | 2005-06-09 |
| EP0682709B1 (de) | 2005-05-04 |
| WO1994018331A3 (en) | 1994-10-13 |
| EP0682709A1 (de) | 1995-11-22 |
| AU6132294A (en) | 1994-08-29 |
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