ATE294958T1 - Quantitatives fluoreszenz-bestimmungsverfahren für transportproteinenaktivität - Google Patents
Quantitatives fluoreszenz-bestimmungsverfahren für transportproteinenaktivitätInfo
- Publication number
- ATE294958T1 ATE294958T1 AT01954227T AT01954227T ATE294958T1 AT E294958 T1 ATE294958 T1 AT E294958T1 AT 01954227 T AT01954227 T AT 01954227T AT 01954227 T AT01954227 T AT 01954227T AT E294958 T1 ATE294958 T1 AT E294958T1
- Authority
- AT
- Austria
- Prior art keywords
- interest
- cells
- transport
- transport protein
- proteins
- Prior art date
Links
- 102000014914 Carrier Proteins Human genes 0.000 title abstract 6
- 108010078791 Carrier Proteins Proteins 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 4
- 230000004952 protein activity Effects 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 5
- 102000004855 Multi drug resistance-associated proteins Human genes 0.000 abstract 2
- 108090001099 Multi drug resistance-associated proteins Proteins 0.000 abstract 2
- 239000012190 activator Substances 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- BQRGNLJZBFXNCZ-UHFFFAOYSA-N calcein am Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)=C(OC(C)=O)C=C1OC1=C2C=C(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(=O)C)C(OC(C)=O)=C1 BQRGNLJZBFXNCZ-UHFFFAOYSA-N 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 abstract 1
- 210000000170 cell membrane Anatomy 0.000 abstract 1
- 239000007850 fluorescent dye Substances 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 230000004807 localization Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU0002557A HUP0002557A3 (en) | 2000-07-03 | 2000-07-03 | Simple quantitative fluorescent assay method for determining the activity of transport proteins of interest |
| PCT/HU2001/000074 WO2002003066A2 (en) | 2000-07-03 | 2001-07-03 | Quantitative fluorescent assay for determining the activity of transport proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE294958T1 true ATE294958T1 (de) | 2005-05-15 |
Family
ID=89978444
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01954227T ATE294958T1 (de) | 2000-07-03 | 2001-07-03 | Quantitatives fluoreszenz-bestimmungsverfahren für transportproteinenaktivität |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7122329B2 (de) |
| EP (1) | EP1299723B1 (de) |
| JP (1) | JP2004502433A (de) |
| AT (1) | ATE294958T1 (de) |
| AU (2) | AU7656901A (de) |
| CA (1) | CA2417556C (de) |
| DE (1) | DE60110598T2 (de) |
| HU (1) | HUP0002557A3 (de) |
| WO (1) | WO2002003066A2 (de) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2007251275A1 (en) | 2006-05-12 | 2007-11-22 | Solvo Biotechnology | Cholesterol loaded insect cell membranes as test systems for ABC transporter proteins |
| JP5175336B2 (ja) * | 2007-04-16 | 2013-04-03 | モメンタ ファーマシューティカルズ インコーポレイテッド | 細胞表面グリコシル化に関連する方法 |
| HU231387B1 (hu) * | 2017-10-25 | 2023-06-28 | Cellpharma Kft. | Fluoreszcens festékfelhalmozódási vizsgálat |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU217108B (hu) * | 1994-08-31 | 1999-11-29 | SOLVO Biotechnológiai Kft. | Eljárás daganatok multidrogrezisztenciáját okozó fehérje aktivitásának in vitro mennyiségi kimutatására biológiai mintákban |
-
2000
- 2000-07-03 HU HU0002557A patent/HUP0002557A3/hu unknown
-
2001
- 2001-07-03 JP JP2002508078A patent/JP2004502433A/ja active Pending
- 2001-07-03 AU AU7656901A patent/AU7656901A/xx active Pending
- 2001-07-03 AT AT01954227T patent/ATE294958T1/de not_active IP Right Cessation
- 2001-07-03 EP EP01954227A patent/EP1299723B1/de not_active Expired - Lifetime
- 2001-07-03 AU AU2001276569A patent/AU2001276569B2/en not_active Ceased
- 2001-07-03 US US10/312,857 patent/US7122329B2/en not_active Expired - Fee Related
- 2001-07-03 DE DE60110598T patent/DE60110598T2/de not_active Expired - Fee Related
- 2001-07-03 CA CA002417556A patent/CA2417556C/en not_active Expired - Fee Related
- 2001-07-03 WO PCT/HU2001/000074 patent/WO2002003066A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| HUP0002557A2 (en) | 2002-08-28 |
| EP1299723A2 (de) | 2003-04-09 |
| US7122329B2 (en) | 2006-10-17 |
| JP2004502433A (ja) | 2004-01-29 |
| DE60110598D1 (de) | 2005-06-09 |
| AU7656901A (en) | 2002-01-14 |
| DE60110598T2 (de) | 2006-01-19 |
| CA2417556A1 (en) | 2002-01-10 |
| HU0002557D0 (en) | 2000-08-28 |
| EP1299723B1 (de) | 2005-05-04 |
| US20040033508A1 (en) | 2004-02-19 |
| HUP0002557A3 (en) | 2004-08-30 |
| CA2417556C (en) | 2008-11-18 |
| AU2001276569B2 (en) | 2005-10-06 |
| WO2002003066A3 (en) | 2002-08-22 |
| WO2002003066A2 (en) | 2002-01-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |