ATE297999T1 - Dna expression in transfizierten zellen und testverfahren in transfiziereen zellen - Google Patents

Dna expression in transfizierten zellen und testverfahren in transfiziereen zellen

Info

Publication number
ATE297999T1
ATE297999T1 AT98901377T AT98901377T ATE297999T1 AT E297999 T1 ATE297999 T1 AT E297999T1 AT 98901377 T AT98901377 T AT 98901377T AT 98901377 T AT98901377 T AT 98901377T AT E297999 T1 ATE297999 T1 AT E297999T1
Authority
AT
Austria
Prior art keywords
transfected cells
dna
expression
vector
cell
Prior art date
Application number
AT98901377T
Other languages
English (en)
Inventor
Catherine Claire Blackburn
Ian Paul Chambers
Alexander L Medvinsky
Hitoshi Niwa
Austin Gerard Smith
Original Assignee
Univ Edinburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Edinburgh filed Critical Univ Edinburgh
Application granted granted Critical
Publication of ATE297999T1 publication Critical patent/ATE297999T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT98901377T 1997-01-24 1998-01-26 Dna expression in transfizierten zellen und testverfahren in transfiziereen zellen ATE297999T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9701492.2A GB9701492D0 (en) 1997-01-24 1997-01-24 Transfection of embryonic stem cells
PCT/GB1998/000216 WO1998032868A2 (en) 1997-01-24 1998-01-26 Dna expression in transfected cells and assays carried out in transfected cells

Publications (1)

Publication Number Publication Date
ATE297999T1 true ATE297999T1 (de) 2005-07-15

Family

ID=10806546

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98901377T ATE297999T1 (de) 1997-01-24 1998-01-26 Dna expression in transfizierten zellen und testverfahren in transfiziereen zellen

Country Status (9)

Country Link
US (1) US20020146689A1 (de)
EP (1) EP1007713B1 (de)
JP (1) JP2001508659A (de)
AT (1) ATE297999T1 (de)
AU (1) AU724398B2 (de)
CA (1) CA2278107A1 (de)
DE (1) DE69830594T2 (de)
GB (1) GB9701492D0 (de)
WO (1) WO1998032868A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060115807A1 (en) * 1997-01-24 2006-06-01 Chambers Ian P Episomal expression system
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
EP1490486A4 (de) * 2002-03-15 2006-05-03 Wisconsin Alumni Res Found Verfahren zur identifizierung von die differenzierung steuernden genen
AU2004281309A1 (en) * 2003-10-16 2005-04-28 The University Court Of The University Of Edinburgh Control of ES cell self-renewal and lineage specification, and medium therefor
KR20130082179A (ko) 2004-02-18 2013-07-18 크로모셀 코포레이션 신호 프로브를 이용하는 방법 및 물질
GB2428041B (en) * 2004-03-29 2008-11-05 Univ Edinburgh Improved episomal expression system
EP3105311A1 (de) 2014-02-10 2016-12-21 Univercells NV System, vorrichtung und verfahren zur herstellung von biomolekülen

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5789653A (en) * 1995-01-10 1998-08-04 University Of Edinburgh Secretory gene trap
EP0832209A1 (de) * 1995-06-07 1998-04-01 ZymoGenetics, Inc. Methode zum einfangen und klonieren der zur förderung der sekretion notwendigen leitsequenzen

Also Published As

Publication number Publication date
EP1007713A2 (de) 2000-06-14
WO1998032868A3 (en) 1998-10-01
AU724398B2 (en) 2000-09-21
DE69830594D1 (de) 2005-07-21
AU5771698A (en) 1998-08-18
EP1007713B1 (de) 2005-06-15
CA2278107A1 (en) 1998-07-30
WO1998032868A2 (en) 1998-07-30
DE69830594T2 (de) 2006-05-04
US20020146689A1 (en) 2002-10-10
JP2001508659A (ja) 2001-07-03
GB9701492D0 (en) 1997-03-12

Similar Documents

Publication Publication Date Title
Olson et al. Vitamin K and γ-carboxyglutamate biosynthesis
DK1115840T3 (da) Afstamningsspecifikke celler og progenitorceller
IL109381A (en) Method of isolating enriching and selectively propagating animal stem cells an animal cell and a vector for use in genetically modifying same
ATE295739T1 (de) ADENOVIRALE VEKTOREN BEINHALTEND EIN PROSTATA SPEZIFISCHES ANTIGEN (PSA) ßRESPONSE ELEMENTß
MX9806064A (es) Composicion de enzimas para disociacion de tejidos.
MX9606476A (es) Inhibicion citoplasmica de expresion de genes.
SG148848A1 (en) Polycistronic expression of antibodies
DK1406998T3 (da) Leverindföjede celler, assays og anvendelser deraf
AU3208599A (en) P53-regulated genes
TR199902426T2 (xx) Bitki b�ceklerinin kontrol�.
DE69530001D1 (de) Verbesserungen von oder in bezug auf endometrialfunktion
AR014891A1 (es) Genes aislados de proteinas de membranas de mamiferos; reactivos relacionados
DE3853474D1 (de) Vektor zur integrationsstellenunabhängigen genexpression in säugetier-wirtszellen.
ATE297999T1 (de) Dna expression in transfizierten zellen und testverfahren in transfiziereen zellen
PT942999E (pt) Construcao armadilha de genes para a identificacao e isolamento de genes
ATE107665T1 (de) Proteaseresistenter pdgf und dessen verwendungen.
ATE351905T1 (de) Mitogene regulatoren
EP0332416A3 (de) Regulierung der Expression in Säugetierzellen
WO2005065348A3 (en) Transactivation system for mammalian cells
ES2123569T3 (es) Regulacion de genes en celulas de mamifero dependiente de ecdiesteroides.
WO2000036113A3 (en) Extending graft survival by heme oxygenase-i expression induced immunomodulation
ATE144791T1 (de) Innerhalb des alpha-locus gelegenes t-zell- rezeptor-gen und dna-konstruktionen
WO1994006919A3 (en) Stably-transformed mammalian cells expressing a regulated, inflammatory cyclooxygenase
BR9913047A (pt) Construção de dna, idioplasma vegetal, vegetal, semente vegetal ou célula vegetal, célula de mamìfero, e, uso de uma construção de dna
BR9708256A (pt) Processo para identificar genes com expresão específica ao síto ou de síto preferido em células de marcacção específicas presentes em um ambiente de células diferente ou não daquele de sua origem e uso do mesmo

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties