ATE299181T1 - STRUCTURAL ANALOGOUS SURFACE LOOP OF FIBROBLAST GROWTH FACTORS - Google Patents

STRUCTURAL ANALOGOUS SURFACE LOOP OF FIBROBLAST GROWTH FACTORS

Info

Publication number
ATE299181T1
ATE299181T1 AT94929876T AT94929876T ATE299181T1 AT E299181 T1 ATE299181 T1 AT E299181T1 AT 94929876 T AT94929876 T AT 94929876T AT 94929876 T AT94929876 T AT 94929876T AT E299181 T1 ATE299181 T1 AT E299181T1
Authority
AT
Austria
Prior art keywords
fibroblast growth
amino acid
growth factors
surface loop
beta
Prior art date
Application number
AT94929876T
Other languages
German (de)
Inventor
Andrew Peter Seddon
Lu-Yuan Li
Peter Boehlen
Magdalena Eisinger
Avner Yayon
Original Assignee
Wyeth Corp
Yeda Res & Dev
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/290,373 external-priority patent/US5491220A/en
Application filed by Wyeth Corp, Yeda Res & Dev filed Critical Wyeth Corp
Application granted granted Critical
Publication of ATE299181T1 publication Critical patent/ATE299181T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factor [FGF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factor [FGF]
    • C07K14/501—Fibroblast growth factor [FGF] acidic FGF [aFGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factor [FGF]
    • C07K14/503—Fibroblast growth factor [FGF] basic FGF [bFGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Prostheses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Structural analogues of fibroblast growth factors have an amino acid sequence replacement in the ninth or tenth beta strand of the factor, or the sequence that corresponds to the surface loop that connects the ninth and tenth beta strands, such that the folding of the molecule is not significantly perturbed. Preferred analogues have the overall secondary and tertiary structure of the original factor, and bind to heparin and a member or members of the fibroblast growth factor receptor family with high affinity. In some embodiments the replacement is with another amino acid sequence such as a loop sequence from another structurally related fibroblast growth factor or an interleukin. The figure discloses amino acid sequences from FGF-1, FGF-2, and IL-1 beta.
AT94929876T 1993-09-24 1994-09-23 STRUCTURAL ANALOGOUS SURFACE LOOP OF FIBROBLAST GROWTH FACTORS ATE299181T1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12697393A 1993-09-24 1993-09-24
US08/290,373 US5491220A (en) 1993-09-24 1994-08-15 Surface loop structural analogues of fibroblast growth factors
PCT/US1994/010800 WO1995008630A1 (en) 1993-09-24 1994-09-23 Surface loop structural analogues of fibroblast growth factors

Publications (1)

Publication Number Publication Date
ATE299181T1 true ATE299181T1 (en) 2005-07-15

Family

ID=26825210

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94929876T ATE299181T1 (en) 1993-09-24 1994-09-23 STRUCTURAL ANALOGOUS SURFACE LOOP OF FIBROBLAST GROWTH FACTORS

Country Status (8)

Country Link
EP (1) EP0730651B1 (en)
JP (1) JPH09503661A (en)
AT (1) ATE299181T1 (en)
AU (1) AU687455B2 (en)
CA (1) CA2172137C (en)
DE (1) DE69434420D1 (en)
NZ (1) NZ274667A (en)
WO (1) WO1995008630A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI0785950T1 (en) * 1994-10-13 2003-08-31 Amgen Inc. Keratinocyte growth factor analogs
SI0785949T1 (en) * 1994-10-13 2003-08-31 Amgen Inc. Method for purifying keratinocyte growth factors
AU722626B2 (en) * 1996-05-20 2000-08-10 Abburi Ramaiah Cosmetic preparation
US6743422B1 (en) 1996-10-15 2004-06-01 Amgen, Inc. Keratinocyte growth factor-2 products
US6274712B1 (en) 1997-12-23 2001-08-14 3-Dimensional Pharmaceuticals, Inc. Analogs of human basic fibroblast growth factor mutated at one or more of the positions glutamute 89, aspartate 101 or leucine 137
WO1999055861A2 (en) * 1998-04-28 1999-11-04 Eisai Co., Ltd. Fibroblast growth factor mutein compositions and methods of use therefor
IL139380A0 (en) * 2000-10-31 2001-11-25 Prochon Biotech Ltd Active variants of fibroblast growth factor
IL149562A0 (en) * 2002-05-09 2002-11-10 Prochon Ltd Fgf variants and methods for use thereof
PL3380508T3 (en) 2015-11-27 2021-07-12 Masarykova Univerzita Thermostable fgf2 polypeptide, use thereof
CN114746436B (en) 2019-11-25 2024-07-02 韩国海洋科学技术院 FGF2 polypeptides with improved temperature stability and protease resistance and uses thereof
KR102428940B1 (en) * 2019-11-25 2022-08-03 한국해양과학기술원 Thermally stable and protease resistant fgf2 polypeptide and use of the same

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2526965B2 (en) * 1987-02-24 1996-08-21 武田薬品工業株式会社 Muteins, DNAs and their uses

Also Published As

Publication number Publication date
EP0730651A1 (en) 1996-09-11
DE69434420D1 (en) 2005-08-11
EP0730651A4 (en) 1997-12-03
NZ274667A (en) 1997-03-24
CA2172137C (en) 1999-12-14
WO1995008630A1 (en) 1995-03-30
AU687455B2 (en) 1998-02-26
AU7878494A (en) 1995-04-10
EP0730651B1 (en) 2005-07-06
JPH09503661A (en) 1997-04-15

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Legal Events

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RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties