ATE299190T1 - Didesoxy farbterminatoren - Google Patents
Didesoxy farbterminatorenInfo
- Publication number
- ATE299190T1 ATE299190T1 AT99905589T AT99905589T ATE299190T1 AT E299190 T1 ATE299190 T1 AT E299190T1 AT 99905589 T AT99905589 T AT 99905589T AT 99905589 T AT99905589 T AT 99905589T AT E299190 T1 ATE299190 T1 AT E299190T1
- Authority
- AT
- Austria
- Prior art keywords
- didesoxy
- terminators
- color
- dye
- dye terminator
- Prior art date
Links
- OAKPWEUQDVLTCN-NKWVEPMBSA-N 2',3'-Dideoxyadenosine-5-triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1CC[C@@H](CO[P@@](O)(=O)O[P@](O)(=O)OP(O)(O)=O)O1 OAKPWEUQDVLTCN-NKWVEPMBSA-N 0.000 abstract 1
- 238000001712 DNA sequencing Methods 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- HDRRAMINWIWTNU-NTSWFWBYSA-N [[(2s,5r)-5-(2-amino-6-oxo-3h-purin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@H]1CC[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HDRRAMINWIWTNU-NTSWFWBYSA-N 0.000 abstract 1
- ARLKCWCREKRROD-POYBYMJQSA-N [[(2s,5r)-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)CC1 ARLKCWCREKRROD-POYBYMJQSA-N 0.000 abstract 1
- URGJWIFLBWJRMF-JGVFFNPUSA-N ddTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)CC1 URGJWIFLBWJRMF-JGVFFNPUSA-N 0.000 abstract 1
- 235000011178 triphosphate Nutrition 0.000 abstract 1
- 239000001226 triphosphate Substances 0.000 abstract 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Plural Heterocyclic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1869598A | 1998-02-04 | 1998-02-04 | |
| PCT/US1999/002104 WO1999040223A1 (en) | 1998-02-04 | 1999-02-02 | Dideoxy dye terminators |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE299190T1 true ATE299190T1 (de) | 2005-07-15 |
Family
ID=21789323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99905589T ATE299190T1 (de) | 1998-02-04 | 1999-02-02 | Didesoxy farbterminatoren |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6949635B1 (de) |
| EP (1) | EP1060264B1 (de) |
| JP (1) | JP4306960B2 (de) |
| AT (1) | ATE299190T1 (de) |
| AU (1) | AU2571799A (de) |
| CA (1) | CA2319777C (de) |
| DE (1) | DE69926057T2 (de) |
| WO (1) | WO1999040223A1 (de) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE299190T1 (de) * | 1998-02-04 | 2005-07-15 | Amersham Biosciences Corp | Didesoxy farbterminatoren |
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6117986A (en) * | 1998-06-10 | 2000-09-12 | Intergen Company, L.P. | Pyrimidines linked to a quencher |
| US7501245B2 (en) | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US6358684B1 (en) * | 1999-08-27 | 2002-03-19 | Pe Corporation | UV excitable fluorescent energy transfer dyes |
| CA2382063A1 (en) * | 1999-09-17 | 2001-03-22 | Shiv Kumar | Charge-modified nucleic acid terminators |
| EP1317464B1 (de) | 2000-10-11 | 2009-05-13 | Applera Corporation | Fluoreszierende nukleobasekonjugate mit anionische linker |
| JP2004523243A (ja) | 2001-03-12 | 2004-08-05 | カリフォルニア インスティチュート オブ テクノロジー | 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置 |
| JP4676654B2 (ja) * | 2001-07-19 | 2011-04-27 | 富士フイルム株式会社 | 蛍光ヌクレオチド及びそれを用いた標識法 |
| JP2007524833A (ja) * | 2003-07-01 | 2007-08-30 | ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン | 骨組織の状態を診断するための方法および装置 |
| US20050119587A1 (en) * | 2003-07-01 | 2005-06-02 | University Of Michigan | Method and apparatus for evaluating connective tissue conditions |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| WO2005080605A2 (en) | 2004-02-19 | 2005-09-01 | Helicos Biosciences Corporation | Methods and kits for analyzing polynucleotide sequences |
| US7476734B2 (en) | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
| US7635562B2 (en) | 2004-05-25 | 2009-12-22 | Helicos Biosciences Corporation | Methods and devices for nucleic acid sequence determination |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US7482120B2 (en) | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| US8227621B2 (en) * | 2005-06-30 | 2012-07-24 | Li-Cor, Inc. | Cyanine dyes and methods of use |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| US7729749B2 (en) * | 2005-09-01 | 2010-06-01 | The Regents Of The University Of Michigan | Method and apparatus for evaluating connective tissue conditions |
| WO2007040589A1 (en) * | 2005-09-16 | 2007-04-12 | The Regents Of The University Of Michigan | Method and system for measuring sub-surface composition of a sample |
| US7397546B2 (en) | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
| US20090246762A1 (en) * | 2008-03-31 | 2009-10-01 | Applera Corporation, Applied Biosystems Group | Nucleic acid terminators incorporating a cationic moiety and methods for their use |
| JP2012524153A (ja) * | 2009-04-17 | 2012-10-11 | ライコア インコーポレイテッド | 置換シアニン染料を用いた蛍光イメージング |
| WO2012054784A1 (en) | 2010-10-20 | 2012-04-26 | Li-Cor, Inc. | Fluorescent imaging with substituted cyanine dyes |
| EP2758475B1 (de) | 2011-09-23 | 2017-04-26 | Illumina Cambridge Limited | Farbstoffe zur markierung von molekularen liganden |
| US8809551B1 (en) | 2013-03-08 | 2014-08-19 | Illumina Cambridge Limited | Polymethine compounds and their use as fluorescent labels |
| GB201408077D0 (en) | 2014-05-07 | 2014-06-18 | Illumina Cambridge Ltd | Polymethine compounds and their use as fluorescent labels |
| GB201508858D0 (en) | 2015-05-22 | 2015-07-01 | Illumina Cambridge Ltd | Polymethine compounds with long stokes shifts and their use as fluorescent labels |
| GB201516987D0 (en) | 2015-09-25 | 2015-11-11 | Illumina Cambridge Ltd | Polymethine compounds and their use as fluorescent labels |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5242796A (en) | 1986-07-02 | 1993-09-07 | E. I. Du Pont De Nemours And Company | Method, system and reagents for DNA sequencing |
| CA1340806C (en) | 1986-07-02 | 1999-11-02 | James Merrill Prober | Method, system and reagents for dna sequencing |
| US5795762A (en) * | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5366860A (en) | 1989-09-29 | 1994-11-22 | Applied Biosystems, Inc. | Spectrally resolvable rhodamine dyes for nucleic acid sequence determination |
| US5187085A (en) | 1990-09-28 | 1993-02-16 | Applied Biosystems, Inc. | Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates) |
| US5614365A (en) * | 1994-10-17 | 1997-03-25 | President & Fellow Of Harvard College | DNA polymerase having modified nucleotide binding site for DNA sequencing |
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US5986086A (en) * | 1997-06-20 | 1999-11-16 | Amersham Pharmacia Biotech Inc. | Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides |
| ATE299190T1 (de) * | 1998-02-04 | 2005-07-15 | Amersham Biosciences Corp | Didesoxy farbterminatoren |
-
1999
- 1999-02-02 AT AT99905589T patent/ATE299190T1/de not_active IP Right Cessation
- 1999-02-02 AU AU25717/99A patent/AU2571799A/en not_active Abandoned
- 1999-02-02 CA CA002319777A patent/CA2319777C/en not_active Expired - Fee Related
- 1999-02-02 DE DE69926057T patent/DE69926057T2/de not_active Expired - Lifetime
- 1999-02-02 JP JP2000530633A patent/JP4306960B2/ja not_active Expired - Fee Related
- 1999-02-02 WO PCT/US1999/002104 patent/WO1999040223A1/en not_active Ceased
- 1999-02-02 EP EP99905589A patent/EP1060264B1/de not_active Expired - Lifetime
-
2000
- 2000-10-26 US US09/699,030 patent/US6949635B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU2571799A (en) | 1999-08-23 |
| DE69926057T2 (de) | 2006-04-20 |
| EP1060264A1 (de) | 2000-12-20 |
| WO1999040223A1 (en) | 1999-08-12 |
| US6949635B1 (en) | 2005-09-27 |
| EP1060264B1 (de) | 2005-07-06 |
| CA2319777C (en) | 2008-01-08 |
| DE69926057D1 (de) | 2005-08-11 |
| JP2002505853A (ja) | 2002-02-26 |
| JP4306960B2 (ja) | 2009-08-05 |
| CA2319777A1 (en) | 1999-08-12 |
| EP1060264A4 (de) | 2001-07-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |