ATE299190T1 - Didesoxy farbterminatoren - Google Patents
Didesoxy farbterminatorenInfo
- Publication number
- ATE299190T1 ATE299190T1 AT99905589T AT99905589T ATE299190T1 AT E299190 T1 ATE299190 T1 AT E299190T1 AT 99905589 T AT99905589 T AT 99905589T AT 99905589 T AT99905589 T AT 99905589T AT E299190 T1 ATE299190 T1 AT E299190T1
- Authority
- AT
- Austria
- Prior art keywords
- didesoxy
- terminators
- color
- dye
- dye terminator
- Prior art date
Links
- OAKPWEUQDVLTCN-NKWVEPMBSA-N 2',3'-Dideoxyadenosine-5-triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1CC[C@@H](CO[P@@](O)(=O)O[P@](O)(=O)OP(O)(O)=O)O1 OAKPWEUQDVLTCN-NKWVEPMBSA-N 0.000 abstract 1
- 238000001712 DNA sequencing Methods 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- HDRRAMINWIWTNU-NTSWFWBYSA-N [[(2s,5r)-5-(2-amino-6-oxo-3h-purin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@H]1CC[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HDRRAMINWIWTNU-NTSWFWBYSA-N 0.000 abstract 1
- ARLKCWCREKRROD-POYBYMJQSA-N [[(2s,5r)-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)CC1 ARLKCWCREKRROD-POYBYMJQSA-N 0.000 abstract 1
- URGJWIFLBWJRMF-JGVFFNPUSA-N ddTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)CC1 URGJWIFLBWJRMF-JGVFFNPUSA-N 0.000 abstract 1
- 235000011178 triphosphate Nutrition 0.000 abstract 1
- 239000001226 triphosphate Substances 0.000 abstract 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Plural Heterocyclic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1869598A | 1998-02-04 | 1998-02-04 | |
| PCT/US1999/002104 WO1999040223A1 (en) | 1998-02-04 | 1999-02-02 | Dideoxy dye terminators |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE299190T1 true ATE299190T1 (de) | 2005-07-15 |
Family
ID=21789323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99905589T ATE299190T1 (de) | 1998-02-04 | 1999-02-02 | Didesoxy farbterminatoren |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6949635B1 (de) |
| EP (1) | EP1060264B1 (de) |
| JP (1) | JP4306960B2 (de) |
| AT (1) | ATE299190T1 (de) |
| AU (1) | AU2571799A (de) |
| CA (1) | CA2319777C (de) |
| DE (1) | DE69926057T2 (de) |
| WO (1) | WO1999040223A1 (de) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE299190T1 (de) * | 1998-02-04 | 2005-07-15 | Amersham Biosciences Corp | Didesoxy farbterminatoren |
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6117986A (en) * | 1998-06-10 | 2000-09-12 | Intergen Company, L.P. | Pyrimidines linked to a quencher |
| US7501245B2 (en) | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US6358684B1 (en) * | 1999-08-27 | 2002-03-19 | Pe Corporation | UV excitable fluorescent energy transfer dyes |
| CA2382063A1 (en) * | 1999-09-17 | 2001-03-22 | Shiv Kumar | Charge-modified nucleic acid terminators |
| CA2425663C (en) | 2000-10-11 | 2009-12-29 | Applera Corporation | Fluorescent nucleobase conjugates having anionic linkers |
| WO2002072892A1 (en) | 2001-03-12 | 2002-09-19 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension |
| JP4676654B2 (ja) * | 2001-07-19 | 2011-04-27 | 富士フイルム株式会社 | 蛍光ヌクレオチド及びそれを用いた標識法 |
| JP2007524833A (ja) * | 2003-07-01 | 2007-08-30 | ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン | 骨組織の状態を診断するための方法および装置 |
| US20050119587A1 (en) * | 2003-07-01 | 2005-06-02 | University Of Michigan | Method and apparatus for evaluating connective tissue conditions |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| EP2248911A1 (de) | 2004-02-19 | 2010-11-10 | Helicos Biosciences Corporation | Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen |
| CA2566806A1 (en) | 2004-05-25 | 2006-01-19 | Helicos Biosciences Corporation | Methods and devices for nucleic acid sequence determination |
| US7476734B2 (en) | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US7482120B2 (en) | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| US8227621B2 (en) * | 2005-06-30 | 2012-07-24 | Li-Cor, Inc. | Cyanine dyes and methods of use |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| US7729749B2 (en) * | 2005-09-01 | 2010-06-01 | The Regents Of The University Of Michigan | Method and apparatus for evaluating connective tissue conditions |
| EP1931969A1 (de) * | 2005-09-16 | 2008-06-18 | The Regents of the University of Michigan | Verfahren und system zur messung der zusammensetzung einer probe unter der oberfläche |
| US7397546B2 (en) | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
| US20090246762A1 (en) * | 2008-03-31 | 2009-10-01 | Applera Corporation, Applied Biosystems Group | Nucleic acid terminators incorporating a cationic moiety and methods for their use |
| AU2010236189B2 (en) * | 2009-04-17 | 2015-01-29 | Li-Cor, Inc. | Fluorescent imaging with substituted cyanine dyes |
| EP2630196B1 (de) | 2010-10-20 | 2017-09-06 | Li-Cor, Inc. | Cyaninfarbstoffe und deren konjugate |
| WO2013041117A1 (en) | 2011-09-23 | 2013-03-28 | Illumina Cambrigde Ltd. | Dyes for labelling molecular ligands |
| US8809551B1 (en) | 2013-03-08 | 2014-08-19 | Illumina Cambridge Limited | Polymethine compounds and their use as fluorescent labels |
| GB201408077D0 (en) | 2014-05-07 | 2014-06-18 | Illumina Cambridge Ltd | Polymethine compounds and their use as fluorescent labels |
| GB201508858D0 (en) | 2015-05-22 | 2015-07-01 | Illumina Cambridge Ltd | Polymethine compounds with long stokes shifts and their use as fluorescent labels |
| GB201516987D0 (en) | 2015-09-25 | 2015-11-11 | Illumina Cambridge Ltd | Polymethine compounds and their use as fluorescent labels |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1340806C (en) | 1986-07-02 | 1999-11-02 | James Merrill Prober | Method, system and reagents for dna sequencing |
| US5242796A (en) | 1986-07-02 | 1993-09-07 | E. I. Du Pont De Nemours And Company | Method, system and reagents for DNA sequencing |
| US5795762A (en) * | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5366860A (en) | 1989-09-29 | 1994-11-22 | Applied Biosystems, Inc. | Spectrally resolvable rhodamine dyes for nucleic acid sequence determination |
| US5187085A (en) | 1990-09-28 | 1993-02-16 | Applied Biosystems, Inc. | Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates) |
| US5614365A (en) * | 1994-10-17 | 1997-03-25 | President & Fellow Of Harvard College | DNA polymerase having modified nucleotide binding site for DNA sequencing |
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US5986086A (en) * | 1997-06-20 | 1999-11-16 | Amersham Pharmacia Biotech Inc. | Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides |
| ATE299190T1 (de) * | 1998-02-04 | 2005-07-15 | Amersham Biosciences Corp | Didesoxy farbterminatoren |
-
1999
- 1999-02-02 AT AT99905589T patent/ATE299190T1/de not_active IP Right Cessation
- 1999-02-02 DE DE69926057T patent/DE69926057T2/de not_active Expired - Lifetime
- 1999-02-02 AU AU25717/99A patent/AU2571799A/en not_active Abandoned
- 1999-02-02 WO PCT/US1999/002104 patent/WO1999040223A1/en not_active Ceased
- 1999-02-02 EP EP99905589A patent/EP1060264B1/de not_active Expired - Lifetime
- 1999-02-02 JP JP2000530633A patent/JP4306960B2/ja not_active Expired - Fee Related
- 1999-02-02 CA CA002319777A patent/CA2319777C/en not_active Expired - Fee Related
-
2000
- 2000-10-26 US US09/699,030 patent/US6949635B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999040223A1 (en) | 1999-08-12 |
| JP4306960B2 (ja) | 2009-08-05 |
| US6949635B1 (en) | 2005-09-27 |
| CA2319777A1 (en) | 1999-08-12 |
| EP1060264A1 (de) | 2000-12-20 |
| JP2002505853A (ja) | 2002-02-26 |
| DE69926057T2 (de) | 2006-04-20 |
| CA2319777C (en) | 2008-01-08 |
| EP1060264A4 (de) | 2001-07-04 |
| DE69926057D1 (de) | 2005-08-11 |
| EP1060264B1 (de) | 2005-07-06 |
| AU2571799A (en) | 1999-08-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |