ATE258992T1 - Mit stickstoffheterozyklen substituierte elektronenmangel-fluoresceinfarbstoffe - Google Patents

Mit stickstoffheterozyklen substituierte elektronenmangel-fluoresceinfarbstoffe

Info

Publication number
ATE258992T1
ATE258992T1 AT01907003T AT01907003T ATE258992T1 AT E258992 T1 ATE258992 T1 AT E258992T1 AT 01907003 T AT01907003 T AT 01907003T AT 01907003 T AT01907003 T AT 01907003T AT E258992 T1 ATE258992 T1 AT E258992T1
Authority
AT
Austria
Prior art keywords
nitrogen heterocycles
fluorescein dyes
electron
electron deficiency
dyes substituted
Prior art date
Application number
AT01907003T
Other languages
English (en)
Inventor
Krishna Upadhya
Steven Menchen
Weiguo Zhen
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Application granted granted Critical
Publication of ATE258992T1 publication Critical patent/ATE258992T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D405/00Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/04Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D405/00Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/14Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing three or more hetero rings
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00Diaryl- or thriarylmethane dyes
    • C09B11/04Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/06Hydroxy derivatives of triarylmethanes in which at least one OH group is bound to an aryl nucleus and their ethers or esters
    • C09B11/08Phthaleins; Phenolphthaleins; Fluorescein
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00Chemistry: analytical and immunological testing
    • Y10S436/80Fluorescent dyes, e.g. rhodamine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pyrane Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Quinoline Compounds (AREA)
  • Pyridine Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
AT01907003T 2000-02-07 2001-02-05 Mit stickstoffheterozyklen substituierte elektronenmangel-fluoresceinfarbstoffe ATE258992T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/498,702 US6221604B1 (en) 2000-02-07 2000-02-07 Electron-deficient nitrogen heterocycle-substituted fluorescein dyes
PCT/US2001/003772 WO2001057264A2 (en) 2000-02-07 2001-02-05 Electron-deficient nitrogen heterocycle-substituted fluorescein dyes

Publications (1)

Publication Number Publication Date
ATE258992T1 true ATE258992T1 (de) 2004-02-15

Family

ID=23982139

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01907003T ATE258992T1 (de) 2000-02-07 2001-02-05 Mit stickstoffheterozyklen substituierte elektronenmangel-fluoresceinfarbstoffe

Country Status (9)

Country Link
US (2) US6221604B1 (de)
EP (1) EP1196640B1 (de)
JP (5) JP2003521571A (de)
AT (1) ATE258992T1 (de)
AU (1) AU772739B2 (de)
CA (1) CA2368495C (de)
DE (1) DE60101939T2 (de)
ES (1) ES2211768T3 (de)
WO (1) WO2001057264A2 (de)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6221604B1 (en) * 2000-02-07 2001-04-24 Pe Corporation Electron-deficient nitrogen heterocycle-substituted fluorescein dyes
CA2425663C (en) * 2000-10-11 2009-12-29 Applera Corporation Fluorescent nucleobase conjugates having anionic linkers
US6887690B2 (en) * 2001-06-22 2005-05-03 Pe Corporation Dye-labeled ribonucleotide triphosphates
US8569516B2 (en) * 2001-09-07 2013-10-29 Elitech Holding B.V. Compounds and methods for fluorescent labeling
US6972339B2 (en) * 2001-09-07 2005-12-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
WO2003040395A2 (en) * 2001-11-07 2003-05-15 Applera Corporation Universal nucleotides for nucleic acid analysis
US20030148284A1 (en) * 2001-12-17 2003-08-07 Vision Todd J. Solid phase detection of nucleic acid molecules
US7026166B2 (en) * 2002-01-22 2006-04-11 Chiron Corporation Fluorogenic dyes
US7074597B2 (en) * 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
US7704756B2 (en) * 2003-01-21 2010-04-27 Novartis Vaccines And Diagnostics, Inc. Fluorogenic dyes
KR20070121853A (ko) 2003-03-31 2007-12-27 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
US7399639B2 (en) * 2003-05-04 2008-07-15 Massachusetts Institute Of Technology Sensors, and methods of making and using the same
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
US20060029948A1 (en) * 2003-09-19 2006-02-09 Gary Lim Sealing cover and dye compatibility selection
US7417726B2 (en) * 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US7541454B2 (en) * 2003-10-24 2009-06-02 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
CA2547418A1 (en) * 2003-12-08 2005-06-23 The Research Foundation Of State University Of New York Site selectively tagged and templated molecularly imprinted polymers for sensor applications
JP4127204B2 (ja) * 2003-12-17 2008-07-30 セイコーエプソン株式会社 液晶表示装置の製造方法
US7598087B2 (en) * 2004-01-07 2009-10-06 The Research Foundation Of State University Of New York Protein imprinted polymers with integrated emission sites
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
EP1748767B1 (de) 2004-05-28 2011-12-28 Unigen, Inc. 1-(3-methyl-2,4-dimethoxyphenyl)-3-(2',4'-dihydroxyphenyl)-propan als hochwirksamer tyrosinase-inhibitor
EP1781675B1 (de) 2004-08-13 2014-03-26 Epoch Biosciences, Inc. Fluoreszierende phosphonatfarbstoffe und konjugate
US7767834B2 (en) * 2004-08-13 2010-08-03 Elitech Holding B.V. Phosphonylated fluorescent dyes and conjugates
EP1886145A2 (de) 2005-05-03 2008-02-13 Applera Corporation Fluoreszenznachweissystem und farbstoffsatz zur verwendung damit
EP2674417A3 (de) * 2007-11-21 2014-04-09 Decode Genetics EHF Biaryl-PDE4-Inhibitoren zur Behandlung von Entzündungen
KR101988329B1 (ko) * 2008-07-21 2019-06-12 유니젠, 인크. 일련의 피부-화이트닝(라이트닝) 화합물
KR101718994B1 (ko) * 2009-09-16 2017-03-23 포항공과대학교 산학협력단 무기 및 유기수은종을 형광을 통하여 감지하는 화학량계의 개발
US20130078148A1 (en) 2010-06-07 2013-03-28 Konica Minolta Holdings, Inc. Near field-enhanced fluorescence sensor chip
WO2012023391A1 (ja) 2010-08-17 2012-02-23 コニカミノルタホールディングス株式会社 非特異吸着の精製機構を備えたspfs用センサ
CN107326073A (zh) 2010-12-29 2017-11-07 生命技术公司 作为荧光参考标准品的 ddao 化合物
JP6216312B2 (ja) 2011-03-24 2017-10-18 ユニゲン・インコーポレーテッド ジアリールプロパンを調製するための化合物及び方法
DK2714939T3 (en) 2011-05-24 2015-06-29 Elitech Holding Bv DETECTION OF METHICILLIN-RESISTENT STAPHYLOCOCCUS AUREUS
CN102321063B (zh) * 2011-07-19 2015-07-01 华东理工大学 一种制备不对称罗丹明的方法
WO2013015282A1 (ja) 2011-07-28 2013-01-31 コニカミノルタホールディングス株式会社 アナライト量の測定方法およびspfs用装置
CN103012354A (zh) * 2012-12-24 2013-04-03 广州医药研究总院 5(6)-羧基荧光素异构体的制备方法
WO2014164479A1 (en) 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
US9328384B2 (en) 2013-05-13 2016-05-03 Elitechgroup B.V. Droplet digital PCR with short minor groove probes
CN103508941B (zh) * 2013-10-29 2015-07-22 郑州大学 2,4-二苯基-5,6-二吡啶基-苯酚及其制备方法
EP3230467A1 (de) 2014-12-12 2017-10-18 ELITechGroup B.V. Verfahren und zusammensetzungen zum nachweis von antibiotikaresistenten bakterien
EP3230468B1 (de) 2014-12-12 2020-09-16 ELITechGroup, Inc. Verfahren und kits zum nachweis von antibiotikaresistenten bakterien
EP3247797B1 (de) 2015-01-12 2020-04-29 Life Technologies Corporation Verfahren, systeme und zusammensetzungen daraus zur kontrolle und quantifizierung der nukleinsäurebibliotheksqualität
JP6675758B2 (ja) * 2015-02-20 2020-04-01 国立大学法人名古屋大学 ホスファフルオレセイン化合物若しくはその塩、又はそれを用いた蛍光色素
WO2019069885A1 (ja) 2017-10-02 2019-04-11 コニカミノルタ株式会社 アナライトの検出方法
CA3124333A1 (en) 2018-12-20 2020-06-25 Life Technologies Corporation Modified rhodamine dye and use thereof in biological assays
MX2021007492A (es) 2018-12-20 2021-10-13 Life Technologies Corp Colorante de rodamina asimétrica y uso de este en ensayos biológicos.
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2021092408A1 (en) * 2019-11-08 2021-05-14 The University Of North Carolina At Charlotte Asymmetric donor-acceptor molecular dyes
JP7570350B2 (ja) 2019-12-06 2024-10-21 大塚製薬株式会社 Psa定量用標準物質、その調製方法、psa定量用標準液及びpsa定量方法
CA3186955A1 (en) 2020-07-23 2022-01-27 Scott Benson Energy transfer dye conjugates for use in biological assays
US20230304932A1 (en) 2020-07-23 2023-09-28 Life Technologies Corporation Compositions, systems and methods for biological analysis involving energy transfer dye conjugates and analytes comprising the same
CN113563360A (zh) * 2021-08-16 2021-10-29 中国科学技术大学 一种两亲性荧光染料及其合成方法和应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4318846A (en) 1979-09-07 1982-03-09 Syva Company Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5654442A (en) * 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
EP0658167B1 (de) 1992-09-03 1999-04-14 The Perkin-Elmer Corporation 4,7-dichlorofluoroszein-farbestoffe als molekulare sonden
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5453505A (en) 1994-06-30 1995-09-26 Biometric Imaging, Inc. N-heteroaromatic ion and iminium ion substituted cyanine dyes for use as fluorescence labels
US6020481A (en) 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5821356A (en) 1996-08-12 1998-10-13 The Perkin Elmer Corporation Propargylethoxyamino nucleotides
US5770716A (en) 1997-04-10 1998-06-23 The Perkin-Elmer Corporation Substituted propargylethoxyamido nucleosides, oligonucleotides and methods for using same
US6130101A (en) * 1997-09-23 2000-10-10 Molecular Probes, Inc. Sulfonated xanthene derivatives
US6008379A (en) * 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US6221604B1 (en) * 2000-02-07 2001-04-24 Pe Corporation Electron-deficient nitrogen heterocycle-substituted fluorescein dyes

Also Published As

Publication number Publication date
ES2211768T3 (es) 2004-07-16
JP4942150B2 (ja) 2012-05-30
JP2007277564A (ja) 2007-10-25
WO2001057264A3 (en) 2002-02-21
EP1196640B1 (de) 2004-02-04
WO2001057264A2 (en) 2001-08-09
CA2368495A1 (en) 2001-08-09
CA2368495C (en) 2009-09-29
JP2006257427A (ja) 2006-09-28
JP2007277565A (ja) 2007-10-25
DE60101939D1 (de) 2004-03-11
EP1196640A2 (de) 2002-04-17
US6221604B1 (en) 2001-04-24
AU3483901A (en) 2001-08-14
AU772739B2 (en) 2004-05-06
USRE39663E1 (en) 2007-05-29
JP2003521571A (ja) 2003-07-15
JP2006070270A (ja) 2006-03-16
DE60101939T2 (de) 2005-01-05

Similar Documents

Publication Publication Date Title
DE60101939D1 (de) Mit stickstoffheterozyklen substituierte elektronenmangel-fluoresceinfarbstoffe
ATE349700T1 (de) Übergangsmetallkomplex-haltige lumineszente proteinfarben
DE60005787D1 (de) Wasserlösliche rhodaminfarbstoffe und ihre konjugate
WO2002026789A3 (en) Nucleotide sequences mediating male fertility and method of using same
BR0301684A (pt) Corantes azo piridona dimérica
IT1319241B1 (it) Oggetto decorativo, in particolare di gioielleria.
WO2002030944A3 (en) Fluorescent nucleobase conjugates having anionic linkers
BR0016334A (pt) Composições de polipeptìdeo com estabilidade aperfeiçoada
NO20022793D0 (no) Amifile polymere samt polypeptidkonjugater inneholdende slike
ES2571852T3 (es) Moléculas mutantes CTLA4 solubles y usos de las mismas
HK1044966A1 (zh) C端蛋白标签
HK1054980A1 (zh) 利用肝脏干细胞进行毒性分型
WO2005047459A3 (en) Sars nucleic acids, proteins, antibodies, and uses thereof
DZ3216A1 (fr) Inhibiteurs tricycliques de poly(adp-ribose) polymerases
WO2001036685A3 (en) Differential gene expression in cancer
ATE505549T1 (de) Männliche fertilitätvermittelnde nukleotidsequenzen und deren verwendung
DE60100620D1 (de) Gezielte elektrochemische reduktion von halogenierten 4-amino-picolinsäuren
ATE284407T1 (de) Pyrimidinderivate als markierte bindungspartner
AU2001269575A1 (en) Nucleic acid sequences and proteins involved in cellular senescence
WO2005014723A8 (en) Cyanin-type compounds having an alkynyl linker arm
FR2781481B1 (fr) Nouveaux 2-amino, 4-alkylamino pyrimidine 3-oxydes et composition les comprenant
PT1023310E (pt) Sintese melhorada de oligonucleotidos sulfurados
WO2005016233A3 (en) Botulinum neurotoxin b receptors and use thereof
DE60010346D1 (de) Als überzüge verwendbare witterungsbeständige zusammensetzungen, verfahren, und damit überzogene artikel
TR200501541T2 (tr) Leu-hirudinin e.coli ile kültür ortamında ayrışarak üretilmesinin sinyal sekansları

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties