ATE301200T1 - Mikrobielle proteinexpressionssysteme - Google Patents

Mikrobielle proteinexpressionssysteme

Info

Publication number
ATE301200T1
ATE301200T1 AT00922689T AT00922689T ATE301200T1 AT E301200 T1 ATE301200 T1 AT E301200T1 AT 00922689 T AT00922689 T AT 00922689T AT 00922689 T AT00922689 T AT 00922689T AT E301200 T1 ATE301200 T1 AT E301200T1
Authority
AT
Austria
Prior art keywords
caf1
mature
signal peptide
protein
subunit
Prior art date
Application number
AT00922689T
Other languages
English (en)
Inventor
Timo Korpela
Sheila Macintyre-Ayane
Anton Vladimirovich Zavialov
Natalia Vsevolodov Battchikova
Lada Evgenievna Petrovskaya
Vyacheslav Grigorievic Korobko
Yalov Vladimir Petrovich Zav
Original Assignee
Biotecnol Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotecnol Sa filed Critical Biotecnol Sa
Application granted granted Critical
Publication of ATE301200T1 publication Critical patent/ATE301200T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AT00922689T 1999-05-04 2000-05-03 Mikrobielle proteinexpressionssysteme ATE301200T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI991014A FI109361B (fi) 1999-05-04 1999-05-04 Mikrobinen proteiinin ilmentämisjärjestelmä
PCT/FI2000/000387 WO2000066756A1 (en) 1999-05-04 2000-05-03 Microbial protein expression system

Publications (1)

Publication Number Publication Date
ATE301200T1 true ATE301200T1 (de) 2005-08-15

Family

ID=8554586

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00922689T ATE301200T1 (de) 1999-05-04 2000-05-03 Mikrobielle proteinexpressionssysteme

Country Status (13)

Country Link
US (1) US6919198B1 (de)
EP (1) EP1173592B1 (de)
AT (1) ATE301200T1 (de)
AU (1) AU777246B2 (de)
CA (1) CA2370436A1 (de)
DE (1) DE60021719T2 (de)
DK (1) DK1173592T3 (de)
ES (1) ES2245306T3 (de)
FI (1) FI109361B (de)
NZ (1) NZ515483A (de)
PT (1) PT1173592E (de)
WO (1) WO2000066756A1 (de)
ZA (1) ZA200109231B (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1077263A1 (de) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
US6677139B1 (en) 1999-12-23 2004-01-13 Genecor International, Inc. Methods for production of proteins in host cells
US7276355B2 (en) 2000-12-26 2007-10-02 Sekisui Chemical Co., Ltd. Process for production of a recombinant protein and a fusion protein
EP1791961B1 (de) * 2004-08-18 2011-06-08 The Governors of the University of Alberta Proteinherstellungsverfahren unter nutzung von yebf
CA2583361C (en) 2004-10-15 2015-08-04 Sekisui Chemical Co., Ltd. Gene immunization using an antigen-chaperonin fusion
EP2858675A2 (de) * 2012-06-11 2015-04-15 University Of Newcastle Upon Tyne Rekombinantes polypeptid
CN110724187B (zh) * 2018-07-16 2022-09-16 甘李药业股份有限公司 一种高效表达利拉鲁肽前体的重组工程菌及其应用
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
US12537071B1 (en) 2020-07-22 2026-01-27 David Gordon Bermudes Bacteria having boolean control pathways expressing therapeutic proteins including immunotherapeutic cytotoxins

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE195347T1 (de) * 1992-10-02 2000-08-15 Res Corp Technologies Inc Methoden der erhöhung der sekretion von überexpremierten proteinen
US5639635A (en) * 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
JPH09173078A (ja) * 1995-09-14 1997-07-08 Tadayuki Imanaka 分子シャペロンを用いる蛋白質の製造方法
JP3344618B2 (ja) * 1997-06-20 2002-11-11 株式会社エイチ・エス・ピー研究所 シャペロン発現プラスミド

Also Published As

Publication number Publication date
ZA200109231B (en) 2002-11-08
CA2370436A1 (en) 2000-11-09
AU4300300A (en) 2000-11-17
EP1173592B1 (de) 2005-08-03
DK1173592T3 (da) 2005-10-10
PT1173592E (pt) 2005-11-30
FI109361B (fi) 2002-07-15
AU777246B2 (en) 2004-10-07
US6919198B1 (en) 2005-07-19
EP1173592A1 (de) 2002-01-23
DE60021719D1 (de) 2005-09-08
FI991014A0 (fi) 1999-05-04
DE60021719T2 (de) 2006-06-08
FI991014L (fi) 2000-11-05
ES2245306T3 (es) 2006-01-01
WO2000066756A1 (en) 2000-11-09
NZ515483A (en) 2003-09-26

Similar Documents

Publication Publication Date Title
He et al. Type III protein secretion mechanism in mammalian and plant pathogens
DE60021719D1 (de) Mikrobielle proteinexpressionssysteme
Chen et al. Novel expression vector for secretion of cecropin AD in Bacillus subtilis with enhanced antimicrobial activity
HUP9800730A2 (hu) Expressziós rendszer heterológ antigének fúziós proteinként történő expresszáltatására
AU2001292749A1 (en) Twin-arginine translocation in bacillus
DK1356073T3 (da) Fermenteringsfremgangsmåde til fremstilling af heterologe genprodukter i mælkesyrebakterier
WO1993023434A3 (en) Bpi-immunoglobulin fusion proteins
NO20033673L (no) Fusjonsprotein for sekresjon av et protein av interesse til supernatanten til en bakteriekultur
WO1997014806A3 (en) Delivery of biologically active polypeptides
WO1999058652A3 (en) Cytotoxin-based biological containment
CY1114413T1 (el) Πρωτεϊνικη μετατοπiση σε φυτικα κυτταρα
EP1392717A4 (de) Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine
EP4374923A3 (de) Chimäre rezeptorbindende proteine zur verwendung in vehikeln zur bakteriellen freisetzung
WO2002096467A3 (en) Pharmaceutical use for secreted bacterial effector proteins
Zhang et al. Improvement of human interferon alpha secretion by Lactococcus lactis
EE200000102A (et) Meetodid ja kompositsioonid teraapiaks, kasutades sekreteeritavaid valke, näiteks interferoon-beetat kodeerivaid geene
GB0116460D0 (en) Biological products
Barthelemy et al. Production and secretion of human interleukin 6 into the periplasm of Escherichia coli: efficient processing of N-terminal variants of hIL6 by the E. coli signal peptidase
AU4016089A (en) The expression of homologous and heterologous proteins, which are able to function, on the outer membrane of e. coli and other gram-negative bacteria
WO2006083516A3 (en) Modified cyanovirin-n polypeptide
Laplace et al. Heterologous signal peptide processing in fusion interferon synthesis by engineered L-forms of Proteus mirabilis
KR20060006204A (ko) 비브리오 패혈증균의 ｐａｓ 인자를 이용한 재조합 융합단백질의 생산 방법
WO2003080660A3 (en) Method for the preparation of recombinant mammalian heparin-binding protein (hbp)
WO2002038739A3 (en) Bacterial expression systems
WO2025037284A3 (en) Genetically modified bacteria for multi-modal secretion of a neoantigen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties