ATE302279T1 - Kälteempfindliche dna-polymerasemutanten - Google Patents

Kälteempfindliche dna-polymerasemutanten

Info

Publication number
ATE302279T1
ATE302279T1 AT01944300T AT01944300T ATE302279T1 AT E302279 T1 ATE302279 T1 AT E302279T1 AT 01944300 T AT01944300 T AT 01944300T AT 01944300 T AT01944300 T AT 01944300T AT E302279 T1 ATE302279 T1 AT E302279T1
Authority
AT
Austria
Prior art keywords
dna polymerases
mutation
cold
dna polymerase
compared
Prior art date
Application number
AT01944300T
Other languages
English (en)
Inventor
Wayne M Barnes
Milko B Kermekchiev
Original Assignee
Univ Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Washington filed Critical Univ Washington
Application granted granted Critical
Publication of ATE302279T1 publication Critical patent/ATE302279T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT01944300T 2000-06-06 2001-06-05 Kälteempfindliche dna-polymerasemutanten ATE302279T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/587,856 US6214557B1 (en) 2000-06-06 2000-06-06 Cold sensitive mutant DNA polymerases
PCT/US2001/018236 WO2001094562A2 (en) 2000-06-06 2001-06-05 Cold sensitive mutant dna polymerases

Publications (1)

Publication Number Publication Date
ATE302279T1 true ATE302279T1 (de) 2005-09-15

Family

ID=24351474

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01944300T ATE302279T1 (de) 2000-06-06 2001-06-05 Kälteempfindliche dna-polymerasemutanten

Country Status (7)

Country Link
US (3) US6214557B1 (de)
EP (1) EP1287146B1 (de)
JP (1) JP2003535587A (de)
AT (1) ATE302279T1 (de)
AU (2) AU6672601A (de)
DE (1) DE60112746T2 (de)
WO (1) WO2001094562A2 (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU785425B2 (en) 2001-03-30 2007-05-17 Genetic Technologies Limited Methods of genomic analysis
US20030027196A1 (en) * 2001-08-02 2003-02-06 Barnes Wayne M. Magnesium precipitate methods for magnesium dependent enzymes
EP1419275A4 (de) * 2001-08-02 2007-05-30 Wayne M Barnes Hot start-magnesiumpräzipitationsverfahren zur molekularen manipulation von nukleinsäuren
US6403341B1 (en) * 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
US20030108919A1 (en) * 2001-09-05 2003-06-12 Perlegen Sciences, Inc. Methods for amplification of nucleic acids
US6898531B2 (en) 2001-09-05 2005-05-24 Perlegen Sciences, Inc. Algorithms for selection of primer pairs
US6740510B2 (en) * 2001-09-05 2004-05-25 Perlegen Sciences, Inc. Methods for amplification of nucleic acids
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
US8394944B2 (en) * 2002-09-20 2013-03-12 Siemens Healthcare Diagnostics Inc. Dual-purpose primers and probes for providing enhanced hybridization assays by disruption of secondary structure formation
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
EP1706502A4 (de) * 2003-11-26 2007-05-23 Eppendorf Ag Verfahren und zusammensetzungen zur in-vitro-amplifikation extrachromosomaler nukleinsäure
US7417133B2 (en) 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
US7462475B2 (en) 2004-05-20 2008-12-09 Dna Poleymerase Technology, Inc. Use of whole blood in PCR reactions
GB2416352B (en) * 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
US20060228726A1 (en) * 2005-01-06 2006-10-12 Martin Patrick K Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
WO2007031952A2 (en) * 2005-09-13 2007-03-22 John Mastin Method of preparing upholstery for installation on an article of furniture
DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
EP2113574A1 (de) 2008-04-28 2009-11-04 Biotype AG Stoffe und Verfahren für Profilierungstest auf DNA-Basis
WO2012032510A1 (en) 2010-09-07 2012-03-15 Yeda Research And Development Co. Ltd. Primers for amplifying dna and methods of selecting same
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
WO2012173905A1 (en) * 2011-06-16 2012-12-20 The Regents Of The Unversity Of California Salt-tolerant dna polymerases
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
US20130040365A1 (en) 2011-08-10 2013-02-14 Life Technologies Corporation Polymerase compositions, methods of making and using same
ES2668448T3 (es) 2011-12-08 2018-05-18 F. Hoffmann-La Roche Ag ADN polimerasas con actividad mejorada
JP6450308B2 (ja) * 2012-06-14 2019-01-09 ライフ テクノロジーズ コーポレーション ポリメラーゼ連鎖反応（ｐｃｒ）のための新規の組成物、方法、およびキット
CN103509767B (zh) * 2012-06-27 2016-08-03 菲鹏生物股份有限公司 一种突变型Taq酶及其制备方法
WO2014023318A1 (en) * 2012-08-08 2014-02-13 Universität Konstanz New dna polymerases with increased substrate scope
CN105039278B (zh) * 2015-06-17 2018-09-11 菲鹏生物股份有限公司 突变型Taq DNA聚合酶及其制备方法和应用
US10865441B2 (en) * 2019-03-13 2020-12-15 Abclonal Science, Inc. Mutant Taq polymerase for faster amplification
CN111826366B (zh) * 2020-06-05 2022-08-02 广州英赞生物科技有限公司 一种直扩型热启动dna聚合酶及其制备方法与应用
CN113257347B (zh) * 2021-05-14 2022-02-11 温州谱希医学检验实验室有限公司 注释后的突变检测结果文件的数据处理方法及相关设备
WO2026078192A1 (en) * 2024-10-10 2026-04-16 Qiagen Gmbh Heat-stable dna polymerases and reverse transcriptases

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554101A (en) 1981-01-09 1985-11-19 New York Blood Center, Inc. Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
AU8906091A (en) * 1990-10-05 1992-04-28 Wayne M. Barnes Thermostable dna polymerase
US5599660A (en) 1993-01-19 1997-02-04 Pharmacia Biotech Inc. Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US6020130A (en) * 1995-06-07 2000-02-01 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands that bind to and inhibit DNA polymerases
CZ293215B6 (cs) * 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
WO1998023733A2 (en) * 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
DE69841023D1 (de) * 1997-03-12 2009-09-10 Applied Biosystems Llc DNA Polymerasen mit verbesserten Fähigkeiten zum Einbau von markierten Nukleotiden
US6346379B1 (en) * 1997-09-11 2002-02-12 F. Hoffman-La Roche Ag Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes

Also Published As

Publication number Publication date
DE60112746T2 (de) 2006-05-18
US6316202B1 (en) 2001-11-13
WO2001094562A3 (en) 2002-05-30
AU2001266726B2 (en) 2006-07-13
AU6672601A (en) 2001-12-17
US6333159B1 (en) 2001-12-25
US6214557B1 (en) 2001-04-10
EP1287146B1 (de) 2005-08-17
JP2003535587A (ja) 2003-12-02
DE60112746D1 (de) 2005-09-22
EP1287146A2 (de) 2003-03-05
US20020004203A1 (en) 2002-01-10
WO2001094562A2 (en) 2001-12-13

Similar Documents

Publication Publication Date Title
ATE302279T1 (de) Kälteempfindliche dna-polymerasemutanten
JP6224689B2 (ja) シトシンとこれの修飾物とを識別するための、およびメチローム分析のための方法および組成物
EP2272952B8 (de) Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität
KR20040028991A (ko) 핵산 증폭 방법
EP1144653A2 (de) Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der in vitro nucleinsäuresynthese und amplifikation
WO2001071042A3 (en) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
DK0528882T3 (da) DNA-amplifikationssystem til påvisning af genetiske sygdomme ved hjælp af termostabil ligase
CA2400049A1 (en) Rna polymerase mutants with increased thermostability
EP1212449A4 (de) Verfahren zur amplifikation von signal-flankierenden sequenzen aus unbekannten, genomischen daten
Baker et al. Identification and properties of microsatellite markers in tsetse flies Glossina morsitans sensu lato (Diptera: Glossinidae)
ATE371022T1 (de) Fy7 polymerase
US10358673B2 (en) Method of amplifying nucleic acid sequences
Blair et al. Isolation and characterization of polymorphic microsatellite markers in Schistosoma mansoni from Africa
WO2006030455A8 (en) Dna polymerases having strand displacement activity
Florin et al. Isolation and characterization of polymorphic microsatellite markers in the blowflies Lucilia illustris and Lucilia sericata
Chelliserrykattil et al. Polymerase Amplification, Cloning, and Gene Expression of Benzo‐Homologous “yDNA” Base Pairs
WO1990009457A3 (en) Oligonucleotide primer pairs for sequence independent gene amplification and methods which employ them
ATE373095T1 (de) Mutierte muskelspezifische enhancer
EP0872561A3 (de) Neisseria gonorrhoae spezifische Oligonukleotide
EP2180051A3 (de) Verfahren für den Nachweis und die Quantifizierung einer endogenen DNA-Sequenz aus Weizen
DE50115782D1 (de) THERMOSTABILE POLYMERASE AUS i THERMOCOCCUS PACIFICUS /i
WO2004016811A3 (en) Methods and kit for genes shuffling using tagged primers
Gutiérrez‐Rodríguez et al. Isolation and characterization of microsatellite loci in the Caribbean gorgonian Pseudopterogorgia elisabethae
AU2001286268B2 (en) Method of forming complex
Moro et al. Microsatellite primers for the Western Pebble‐mound Mouse (Pseudomys chapmani) that show cross amplification for other species of Australian rodent

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1287146

Country of ref document: EP