ATE302279T1 - Kälteempfindliche dna-polymerasemutanten - Google Patents

Kälteempfindliche dna-polymerasemutanten

Info

Publication number
ATE302279T1
ATE302279T1 AT01944300T AT01944300T ATE302279T1 AT E302279 T1 ATE302279 T1 AT E302279T1 AT 01944300 T AT01944300 T AT 01944300T AT 01944300 T AT01944300 T AT 01944300T AT E302279 T1 ATE302279 T1 AT E302279T1
Authority
AT
Austria
Prior art keywords
dna polymerases
mutation
cold
dna polymerase
compared
Prior art date
Application number
AT01944300T
Other languages
English (en)
Inventor
Wayne M Barnes
Milko B Kermekchiev
Original Assignee
Univ Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Washington filed Critical Univ Washington
Application granted granted Critical
Publication of ATE302279T1 publication Critical patent/ATE302279T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT01944300T 2000-06-06 2001-06-05 Kälteempfindliche dna-polymerasemutanten ATE302279T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/587,856 US6214557B1 (en) 2000-06-06 2000-06-06 Cold sensitive mutant DNA polymerases
PCT/US2001/018236 WO2001094562A2 (en) 2000-06-06 2001-06-05 Cold sensitive mutant dna polymerases

Publications (1)

Publication Number Publication Date
ATE302279T1 true ATE302279T1 (de) 2005-09-15

Family

ID=24351474

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01944300T ATE302279T1 (de) 2000-06-06 2001-06-05 Kälteempfindliche dna-polymerasemutanten

Country Status (7)

Country Link
US (3) US6214557B1 (de)
EP (1) EP1287146B1 (de)
JP (1) JP2003535587A (de)
AT (1) ATE302279T1 (de)
AU (2) AU6672601A (de)
DE (1) DE60112746T2 (de)
WO (1) WO2001094562A2 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
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AU785425B2 (en) * 2001-03-30 2007-05-17 Genetic Technologies Limited Methods of genomic analysis
US6403341B1 (en) * 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
US20030027196A1 (en) * 2001-08-02 2003-02-06 Barnes Wayne M. Magnesium precipitate methods for magnesium dependent enzymes
AU2002327421A1 (en) * 2001-08-02 2003-02-17 Wayne M. Barnes Magnesium precipitate hot start method for molecular manipulation of nucleic acids
US6740510B2 (en) * 2001-09-05 2004-05-25 Perlegen Sciences, Inc. Methods for amplification of nucleic acids
WO2003021259A1 (en) * 2001-09-05 2003-03-13 Perlegen Sciences, Inc. Selection of primer pairs
US6898531B2 (en) 2001-09-05 2005-05-24 Perlegen Sciences, Inc. Algorithms for selection of primer pairs
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
US8394944B2 (en) * 2002-09-20 2013-03-12 Siemens Healthcare Diagnostics Inc. Dual-purpose primers and probes for providing enhanced hybridization assays by disruption of secondary structure formation
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
EP1706502A4 (de) * 2003-11-26 2007-05-23 Eppendorf Ag Verfahren und zusammensetzungen zur in-vitro-amplifikation extrachromosomaler nukleinsäure
US7417133B2 (en) 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
US7462475B2 (en) 2004-05-20 2008-12-09 Dna Poleymerase Technology, Inc. Use of whole blood in PCR reactions
GB2416352B (en) * 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
EP1836319B1 (de) * 2005-01-06 2012-09-19 Life Technologies Corporation Polypeptide mit nukleinsäurebindender aktivität sowie verfahren zur nukleinsäureamplifikation
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US20070210043A1 (en) * 2005-09-13 2007-09-13 Mastin John J Method of preparing upholstery for installation on an article of furniture
DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
EP2113574A1 (de) 2008-04-28 2009-11-04 Biotype AG Stoffe und Verfahren für Profilierungstest auf DNA-Basis
WO2012032510A1 (en) 2010-09-07 2012-03-15 Yeda Research And Development Co. Ltd. Primers for amplifying dna and methods of selecting same
CA2831180C (en) * 2011-04-11 2017-02-14 Keith Bauer Dna polymerases with improved activity
US10059984B2 (en) 2011-06-16 2018-08-28 The Regents Of The University Of California Salt-tolerant DNA polymerases
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
CN103857805B (zh) 2011-08-10 2017-07-18 生命技术公司 聚合酶组合物、制备和使用所述聚合酶组合物的方法
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
JP6144697B2 (ja) 2011-12-08 2017-06-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
KR102264617B1 (ko) * 2012-06-14 2021-06-14 라이프 테크놀로지스 코포레이션 폴리머라제 연쇄 반응 (pcr)을 위한 신규 조성물, 방법 및 키트
CN103509767B (zh) * 2012-06-27 2016-08-03 菲鹏生物股份有限公司 一种突变型Taq酶及其制备方法
WO2014023318A1 (en) * 2012-08-08 2014-02-13 Universität Konstanz New dna polymerases with increased substrate scope
CN105039278B (zh) * 2015-06-17 2018-09-11 菲鹏生物股份有限公司 突变型Taq DNA聚合酶及其制备方法和应用
US10865441B2 (en) * 2019-03-13 2020-12-15 Abclonal Science, Inc. Mutant Taq polymerase for faster amplification
CN111826366B (zh) * 2020-06-05 2022-08-02 广州英赞生物科技有限公司 一种直扩型热启动dna聚合酶及其制备方法与应用
CN113257347B (zh) * 2021-05-14 2022-02-11 温州谱希医学检验实验室有限公司 注释后的突变检测结果文件的数据处理方法及相关设备

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554101A (en) 1981-01-09 1985-11-19 New York Blood Center, Inc. Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
EP0553264A4 (en) 1990-10-05 1994-07-13 Wayne M Barnes Thermostable dna polymerase
US5599660A (en) 1993-01-19 1997-02-04 Pharmacia Biotech Inc. Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
EP0833944B1 (de) * 1995-06-07 2009-01-07 Gilead Sciences, Inc. Nukleinsäure liganden die dna-polymerase binden und inhibieren
CZ293215B6 (cs) * 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
WO1998023733A2 (en) * 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
EP2208789B1 (de) * 1997-03-12 2015-07-22 Applied Biosystems, LLC DNA Polymerasen mit verbesserten Eigenschaften für den Einbau markierter Nukleotide
US6346379B1 (en) * 1997-09-11 2002-02-12 F. Hoffman-La Roche Ag Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes

Also Published As

Publication number Publication date
AU6672601A (en) 2001-12-17
AU2001266726B2 (en) 2006-07-13
DE60112746D1 (de) 2005-09-22
JP2003535587A (ja) 2003-12-02
US20020004203A1 (en) 2002-01-10
US6316202B1 (en) 2001-11-13
US6214557B1 (en) 2001-04-10
EP1287146A2 (de) 2003-03-05
EP1287146B1 (de) 2005-08-17
DE60112746T2 (de) 2006-05-18
WO2001094562A3 (en) 2002-05-30
US6333159B1 (en) 2001-12-25
WO2001094562A2 (en) 2001-12-13

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