ATE302788T1 - Herstellung und verwendung von normalisierten dna-bibliotheken - Google Patents

Herstellung und verwendung von normalisierten dna-bibliotheken

Info

Publication number
ATE302788T1
ATE302788T1 AT97930172T AT97930172T ATE302788T1 AT E302788 T1 ATE302788 T1 AT E302788T1 AT 97930172 T AT97930172 T AT 97930172T AT 97930172 T AT97930172 T AT 97930172T AT E302788 T1 ATE302788 T1 AT E302788T1
Authority
AT
Austria
Prior art keywords
genomic dna
normalized
environmental sample
preparation
isolated
Prior art date
Application number
AT97930172T
Other languages
English (en)
Inventor
Jay M Short
Eric J Mathur
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diversa Corp filed Critical Diversa Corp
Application granted granted Critical
Publication of ATE302788T1 publication Critical patent/ATE302788T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT97930172T 1996-06-18 1997-06-18 Herstellung und verwendung von normalisierten dna-bibliotheken ATE302788T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/665,565 US5763239A (en) 1996-06-18 1996-06-18 Production and use of normalized DNA libraries
PCT/US1997/010748 WO1997048717A1 (en) 1996-06-18 1997-06-18 Production and use of normalized dna libraries

Publications (1)

Publication Number Publication Date
ATE302788T1 true ATE302788T1 (de) 2005-09-15

Family

ID=24670627

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97930172T ATE302788T1 (de) 1996-06-18 1997-06-18 Herstellung und verwendung von normalisierten dna-bibliotheken

Country Status (10)

Country Link
US (4) US5763239A (de)
EP (2) EP1528067A3 (de)
JP (1) JP4227196B2 (de)
AT (1) ATE302788T1 (de)
AU (1) AU718573B2 (de)
CA (1) CA2258175A1 (de)
DE (1) DE69734063T2 (de)
DK (1) DK0923598T3 (de)
ES (1) ES2248851T3 (de)
WO (1) WO1997048717A1 (de)

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Also Published As

Publication number Publication date
EP1528067A2 (de) 2005-05-04
EP0923598B1 (de) 2005-08-24
US6444426B1 (en) 2002-09-03
AU3406697A (en) 1998-01-07
JP2000513933A (ja) 2000-10-24
WO1997048717A1 (en) 1997-12-24
AU718573B2 (en) 2000-04-13
ES2248851T3 (es) 2006-03-16
US6001574A (en) 1999-12-14
DE69734063T2 (de) 2006-06-22
EP1528067A3 (de) 2006-02-01
EP0923598A1 (de) 1999-06-23
EP0923598A4 (de) 2000-07-19
US20030104440A1 (en) 2003-06-05
JP4227196B2 (ja) 2009-02-18
DK0923598T3 (da) 2006-01-09
DE69734063D1 (de) 2005-09-29
CA2258175A1 (en) 1997-12-24
US5763239A (en) 1998-06-09

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