ATE309390T1 - Matrizen von biomolekülen und ihre verwendung in sequenzierung - Google Patents

Matrizen von biomolekülen und ihre verwendung in sequenzierung

Info

Publication number
ATE309390T1
ATE309390T1 AT99936809T AT99936809T ATE309390T1 AT E309390 T1 ATE309390 T1 AT E309390T1 AT 99936809 T AT99936809 T AT 99936809T AT 99936809 T AT99936809 T AT 99936809T AT E309390 T1 ATE309390 T1 AT E309390T1
Authority
AT
Austria
Prior art keywords
biomolecules
matrices
sequencing
arrays
array
Prior art date
Application number
AT99936809T
Other languages
English (en)
Inventor
Shankar Balasubramanian
David Klenerman
Colin Barnes
Mark Allen Osborne
Original Assignee
Solexa Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26151376&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE309390(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GBGB9822670.7A external-priority patent/GB9822670D0/en
Application filed by Solexa Ltd filed Critical Solexa Ltd
Application granted granted Critical
Publication of ATE309390T1 publication Critical patent/ATE309390T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/0063—Other, e.g. van der Waals forces, hydrogen bonding
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
    • B01J2219/00648—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of solid beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00677—Ex-situ synthesis followed by deposition on the substrate
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
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    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B01J2219/00707—Processes involving means for analysing and characterising the products separated from the reactor apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
AT99936809T 1998-07-30 1999-07-30 Matrizen von biomolekülen und ihre verwendung in sequenzierung ATE309390T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP98306094 1998-07-30
GBGB9822670.7A GB9822670D0 (en) 1998-10-16 1998-10-16 Polynucleotide arrays
PCT/GB1999/002487 WO2000006770A1 (en) 1998-07-30 1999-07-30 Arrayed biomolecules and their use in sequencing

Publications (1)

Publication Number Publication Date
ATE309390T1 true ATE309390T1 (de) 2005-11-15

Family

ID=26151376

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99936809T ATE309390T1 (de) 1998-07-30 1999-07-30 Matrizen von biomolekülen und ihre verwendung in sequenzierung

Country Status (10)

Country Link
US (1) US20050042649A1 (de)
EP (1) EP1105529B2 (de)
JP (1) JP2002521064A (de)
AT (1) ATE309390T1 (de)
AU (1) AU770831B2 (de)
CA (1) CA2339121A1 (de)
DE (1) DE69928265T3 (de)
IL (1) IL141148A0 (de)
IS (1) IS5831A (de)
WO (1) WO2000006770A1 (de)

Families Citing this family (246)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1009802B1 (de) 1997-02-12 2004-08-11 Eugene Y. Chan Verfahren zur analyse von polymeren
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
WO2001073121A1 (en) 2000-03-30 2001-10-04 Toyota Jidosha Kabushiki Kaisha Method of determining base sequence of single nucleic acid molecule
US8399383B2 (en) * 2000-05-04 2013-03-19 Yale University Protein chips for high throughput screening of protein activity
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
GB0016473D0 (en) * 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
ATE377093T1 (de) 2000-07-07 2007-11-15 Visigen Biotechnologies Inc Sequenzbestimmung in echtzeit
US20020037359A1 (en) 2000-09-25 2002-03-28 Mutz Mitchell W. Focused acoustic energy in the preparation of peptide arrays
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7419833B2 (en) 2000-11-17 2008-09-02 Nagayama Ip Holdings Llc Method for nucleic acid sequencing
JP2002153271A (ja) 2000-11-17 2002-05-28 Jeol Ltd Ｄｎａあるいはｒｎａの塩基配列決定方法およびｄｎａシーケンサー
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AU2002231933A1 (en) * 2001-01-30 2002-08-12 Solexa Ltd. The preparation of polynucleotide arrays
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
DK1370690T3 (da) 2001-03-16 2012-07-09 Kalim Mir Arrays og fremgangsmåder til anvendelse heraf
EP1249499A1 (de) * 2001-04-10 2002-10-16 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren und Vorrichtung zur Bestimmung und Selektion von Molekül-Molekül-Wechselwirkungen
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
WO2003020968A2 (de) * 2001-08-29 2003-03-13 Genovoxx Gmbh Verfahren zur analyse von nukleinsäurekettensequenzen und der genexpression
GB0123120D0 (en) * 2001-09-26 2001-11-14 Amersham Pharm Biotech Uk Ltd Method of attachment
US6956114B2 (en) 2001-10-30 2005-10-18 '454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
EP2607369B1 (de) 2002-08-23 2015-09-23 Illumina Cambridge Limited Modifizierte Nucleotide zur Polynucleotidsequenzierung
AU2003259352A1 (en) 2002-08-23 2004-03-11 Solexa Limited Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
AU2003267583A1 (en) * 2002-09-19 2004-04-08 The Chancellor, Master And Scholars Of The University Of Oxford Molecular arrays and single molecule detection
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
CA2513889A1 (en) 2003-01-29 2004-08-19 454 Corporation Double ended sequencing
CA2897376A1 (en) * 2003-02-26 2004-09-10 Radoje T. Drmanac Modular system and probes for dna analysis
US20040248323A1 (en) 2003-06-09 2004-12-09 Protometrix, Inc. Methods for conducting assays for enzyme activity on protein microarrays
AT501110A1 (de) * 2003-09-16 2006-06-15 Upper Austrian Res Gmbh Arrays zur bindung von molekülen
AT414047B (de) * 2003-09-16 2006-08-15 Upper Austrian Res Gmbh Anordnung zur bindung von molekülen
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20110059865A1 (en) 2004-01-07 2011-03-10 Mark Edward Brennan Smith Modified Molecular Arrays
EP2248911A1 (de) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
CA2566806A1 (en) 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
EP1828412B2 (de) † 2004-12-13 2019-01-09 Illumina Cambridge Limited Verbessertes nukleotidnachweisverfahren
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
CN101184853B (zh) 2005-03-29 2012-07-04 阿普尔拉股份有限公司 用于分析核酸的基于纳米线的系统
AU2006259565B2 (en) 2005-06-15 2011-01-06 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
US7805081B2 (en) 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US7763423B2 (en) 2005-09-30 2010-07-27 Pacific Biosciences Of California, Inc. Substrates having low density reactive groups for monitoring enzyme activity
US7960104B2 (en) * 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
WO2007133831A2 (en) * 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
US20070168197A1 (en) * 2006-01-18 2007-07-19 Nokia Corporation Audio coding
US7692783B2 (en) 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7715001B2 (en) 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7995202B2 (en) 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
US7563574B2 (en) 2006-03-31 2009-07-21 Pacific Biosciences Of California, Inc. Methods, systems and compositions for monitoring enzyme activity and applications thereof
WO2007123744A2 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
US20090253581A1 (en) 2006-04-04 2009-10-08 Keygene N.V. High Throughput Detection of Molecular Markers Based on AFLP and High Throughput Sequencing
EP2029779A4 (de) 2006-06-14 2010-01-20 Living Microsystems Inc Verwendung hoch paralleler snp-genotypisierung zur fötalen diagnose
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
EP2589668A1 (de) 2006-06-14 2013-05-08 Verinata Health, Inc Analyse seltener Zellen mittels Probentrennung und DNA-Etiketten
DE602007009233D1 (de) 2006-07-12 2010-10-28 Keygene Nv Genomische kartierung mit hohem durchsatz unter verwendung von aflp
US8207509B2 (en) 2006-09-01 2012-06-26 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
WO2008028160A2 (en) 2006-09-01 2008-03-06 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
WO2008045158A1 (en) 2006-10-10 2008-04-17 Illumina, Inc. Compositions and methods for representational selection of nucleic acids fro complex mixtures using hybridization
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US20080221832A1 (en) * 2006-11-09 2008-09-11 Complete Genomics, Inc. Methods for computing positional base probabilities using experminentals base value distributions
DE112007002932B4 (de) 2006-12-01 2015-08-06 The Trustees Of Columbia University In The City Of New York Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren
CA2941187C (en) 2007-08-14 2021-03-02 Fred Hutchinson Cancer Research Center Needle array assembly and method for delivering therapeutic agents
ES2448426T3 (es) 2007-10-12 2014-03-13 Pronota Nv Uso de aptámeros en proteómica
WO2009052214A2 (en) * 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
EP4310194A3 (de) 2007-10-19 2024-10-16 The Trustees of Columbia University in the City of New York Entwurf und synthese von spaltbaren fluoreszierenden nukleotiden als reversible terminatoren zur dna-sequenzierung durch synthese
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8298768B2 (en) 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US7901890B2 (en) * 2007-11-05 2011-03-08 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US8617811B2 (en) 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
WO2009094583A1 (en) * 2008-01-23 2009-07-30 Complete Genomics, Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
US8202691B2 (en) * 2008-01-25 2012-06-19 Illumina, Inc. Uniform fragmentation of DNA using binding proteins
CA2718905A1 (en) 2008-03-17 2009-09-24 Expressive Research B.V. Expression-linked gene discovery
EP3269824A1 (de) 2008-03-28 2018-01-17 Pacific Biosciences Of California, Inc. Zusammensetzungen und verfahren zur nukleinsäuresequenzierung
WO2009132028A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
DK3629011T3 (da) 2008-09-16 2024-01-29 Pacific Biosciences California Inc Integreret optisk indretning
WO2010036287A1 (en) 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8541207B2 (en) * 2008-10-22 2013-09-24 Illumina, Inc. Preservation of information related to genomic DNA methylation
KR101088885B1 (ko) * 2008-12-23 2011-12-07 연세대학교 산학협력단 바이오프로브, 그 제조방법, 상기를 사용한 분석 장치 및 분석 방법
ES2403312T3 (es) 2009-01-13 2013-05-17 Keygene N.V. Nuevas estrategias para la secuenciación del genoma
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
US10036063B2 (en) 2009-07-24 2018-07-31 Illumina, Inc. Method for sequencing a polynucleotide template
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
US8994946B2 (en) 2010-02-19 2015-03-31 Pacific Biosciences Of California, Inc. Integrated analytical system and method
EP3460458B1 (de) 2010-02-19 2021-08-11 Pacific Biosciences of California, Inc. Verfahren zur nukleinsäuresequenzierung
US8834847B2 (en) 2010-08-12 2014-09-16 Pacific Biosciences Of California, Inc. Photodamage mitigation compounds and systems
JP5917519B2 (ja) 2010-09-10 2016-05-18 バイオ−ラッド ラボラトリーズ インコーポレーティッド クロマチン分析のためのｄｎａのサイズ選択
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
US20140235474A1 (en) 2011-06-24 2014-08-21 Sequenom, Inc. Methods and processes for non invasive assessment of a genetic variation
AU2012281242B2 (en) 2011-07-08 2016-12-22 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20140242588A1 (en) 2011-10-06 2014-08-28 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
AU2012328457A1 (en) 2011-10-28 2014-04-24 Presage Biosciences, Inc. Methods for drug delivery
EP2805280B1 (de) 2012-01-20 2022-10-05 Sequenom, Inc. Diagnostische verfahren zur evaluierung von versuchsbedingungen
CA2867489A1 (en) 2012-03-30 2013-10-03 Pacific Biosciences Of California, Inc. Methods and composition for sequencing modified nucleic acids
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US9372308B1 (en) 2012-06-17 2016-06-21 Pacific Biosciences Of California, Inc. Arrays of integrated analytical devices and methods for production
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
EP2936222B1 (de) 2012-12-18 2019-07-03 Pacific Biosciences Of California, Inc. Eine optische analysevorrichtung
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2959283B1 (de) 2013-02-22 2022-08-17 Pacific Biosciences of California, Inc. Integrierte beleuchtung von optischen analysevorrichtungen
EP2964612B1 (de) 2013-03-08 2017-02-01 Illumina Cambridge Limited Polymethinverbindungen und deren verwendung als fluoreszierende markierungen
WO2014135223A1 (en) 2013-03-08 2014-09-12 Illumina Cambridge Ltd Rhodamine compounds and their use as fluorescent labels
US9873913B2 (en) 2013-03-08 2018-01-23 Roche Molecular Systems, Inc. Mutation testing
WO2014143158A1 (en) 2013-03-13 2014-09-18 The Broad Institute, Inc. Compositions and methods for labeling of agents
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
KR102230831B1 (ko) 2013-03-15 2021-03-22 일루미나 케임브리지 리미티드 변형된 뉴클레오사이드 또는 뉴클레오타이드
EP4187543A1 (de) 2013-04-03 2023-05-31 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
US10699800B2 (en) 2013-05-24 2020-06-30 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
KR102299305B1 (ko) 2013-06-21 2021-09-06 시쿼넘, 인코포레이티드 유전적 변이의 비침습 평가를 위한 방법 및 프로세스
MX371428B (es) 2013-08-19 2020-01-30 Singular Bio Inc Ensayos para la deteccion de una sola molecula y uso de los mismos.
DK3053071T3 (da) 2013-10-04 2024-01-22 Sequenom Inc Fremgangsmåder og processer til ikke-invasiv bedømmelse af genetiske variationer
EP3495496B1 (de) 2013-10-07 2020-11-25 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung chromosomaler veränderungen
US10537889B2 (en) 2013-12-31 2020-01-21 Illumina, Inc. Addressable flow cell using patterned electrodes
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
EP3175000B1 (de) 2014-07-30 2020-07-29 Sequenom, Inc. Verfahren und prozesse zur nicht-invasiven beurteilung genetischer variationen
CN107003241B (zh) 2014-08-27 2022-01-11 加利福尼亚太平洋生物科学股份有限公司 集成分析器件阵列
AU2016219943A1 (en) 2015-02-18 2017-10-12 Singular Bio, Inc. Assays for single molecule detection and use thereof
US10487356B2 (en) 2015-03-16 2019-11-26 Pacific Biosciences Of California, Inc. Integrated devices and systems for free-space optical coupling
US11983790B2 (en) 2015-05-07 2024-05-14 Pacific Biosciences Of California, Inc. Multiprocessor pipeline architecture
GB201508858D0 (en) 2015-05-22 2015-07-01 Illumina Cambridge Ltd Polymethine compounds with long stokes shifts and their use as fluorescent labels
EP4425153A3 (de) 2015-06-12 2024-11-20 Pacific Biosciences Of California, Inc. Integrierte zielwellenleitervorrichtungen und systeme zur optischen kopplung
WO2017042040A1 (en) 2015-09-09 2017-03-16 Qiagen Gmbh Polymerase enzyme
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
US10465232B1 (en) 2015-10-08 2019-11-05 Trace Genomics, Inc. Methods for quantifying efficiency of nucleic acid extraction and detection
US11200963B2 (en) 2016-07-27 2021-12-14 Sequenom, Inc. Genetic copy number alteration classifications
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
CN109476674B (zh) 2016-12-22 2021-12-10 伊鲁米纳剑桥有限公司 香豆素化合物及其作为荧光标记物的用途
WO2018140521A1 (en) 2017-01-24 2018-08-02 Sequenom, Inc. Methods and processes for assessment of genetic variations
EP3580351A1 (de) 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymeraseenzym aus 9°n
WO2018148727A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from 9°n
WO2018148726A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from phage t4
WO2018148723A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus abyssi
WO2018148724A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus furiosus
PT3596233T (pt) 2017-03-17 2022-08-22 Sequenom Inc Métodos e processos para avaliação de mosaicismo genético
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
CN118086478A (zh) 2017-08-01 2024-05-28 深圳华大智造科技有限公司 核酸测序方法
WO2019036055A2 (en) 2017-08-18 2019-02-21 Ignite Biosciences, Inc. METHODS OF SELECTING BINDING REAGENTS
CN111148847B (zh) 2017-10-11 2024-05-07 深圳华大智造科技有限公司 用于改善核酸装载和稳定性的方法
GB201716931D0 (en) 2017-10-16 2017-11-29 Illumina Cambridge Ltd New fluorescent compounds and their use as biomarkers
CN112840035B (zh) 2018-11-07 2024-01-30 青岛华大智造科技有限责任公司 对多核苷酸进行测序的方法
US11993865B2 (en) 2018-11-20 2024-05-28 Nautilus Subsidiary, Inc. Selection of affinity reagents
ES2988083T3 (es) 2018-12-19 2024-11-19 Hoffmann La Roche Nucleótidos protegidos reversiblemente en 3' para secuenciación por síntesis usando nanoporos
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
MY209343A (en) 2019-03-01 2025-07-03 Illumina Cambridge Ltd Exocyclic amine substituted coumarin compounds and uses as fluorescent labels
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
KR102865446B1 (ko) 2019-03-01 2025-09-29 일루미나 케임브리지 리미티드 삼차 아민 치환된 쿠마린 화합물 및 형광 표지로서의 이의 용도
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
EP3963091A4 (de) 2019-04-29 2023-07-19 Nautilus Biotechnology, Inc. Verfahren und systeme zur integrierten on-chip-einzelmoleküldetektion
CN118931711A (zh) 2019-05-15 2024-11-12 青岛华大智造科技有限责任公司 单通道测序装置和测序方法
AU2019462785A1 (en) 2019-08-20 2022-04-07 Qingdao MGI Tech Co. Ltd Method for sequencing polynucleotides on basis of optical signal dynamics of luminescent label and secondary luminescent signal
JP2023510454A (ja) 2019-11-27 2023-03-14 イルミナ ケンブリッジ リミテッド シクロオクタテトラエンを含有する色素及び組成物
AU2021222009A1 (en) 2020-02-18 2022-09-15 Pacific Biosciences Of California, Inc. Highly multiplexed nucleic acid sequencing systems
WO2021259881A1 (en) 2020-06-22 2021-12-30 Illumina Cambridge Limited Nucleosides and nucleotides with 3' acetal blocking group
US11981964B2 (en) 2020-07-28 2024-05-14 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
US20230295696A1 (en) 2020-07-29 2023-09-21 Mgi Tech Co., Ltd. Method for loading nucleic acid molecule on solid support
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
US20220195517A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Long stokes shift chromenoquinoline dyes and uses in sequencing applications
US20220195196A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Alkylpyridinium coumarin dyes and uses in sequencing applications
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
US12535417B2 (en) 2021-05-04 2026-01-27 Pacific Biosciences Of California, Inc. Arrays of integrated analytical devices with reduced-scale unit cell
JP2024516191A (ja) 2021-05-05 2024-04-12 イルミナ ケンブリッジ リミテッド ビス－ホウ素縮合複素環を含有する蛍光色素及び配列決定における使用
CN117916390A (zh) 2021-05-20 2024-04-19 伊鲁米纳公司 用于边合成边测序的组合物和方法
CN118076747A (zh) 2021-10-11 2024-05-24 深圳华大智造科技股份有限公司 皂苷类化合物在核酸测序中的用途
US12612656B2 (en) 2021-11-30 2026-04-28 Nautilus Subsidiary, Inc. Particle-based isolation of proteins and other analytes
US20250163491A1 (en) 2021-12-16 2025-05-22 Illumina, Inc. Methods for metal directed cleavage of surface-bound polynucleotides
US20240401127A1 (en) 2021-12-20 2024-12-05 Illumina, Inc. Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
AU2022421156A1 (en) 2021-12-20 2024-01-18 Illumina, Inc. Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
EP4499862A1 (de) 2022-03-28 2025-02-05 Illumina, Inc. Markiertes avidin und sequenzierungsverfahren
AU2023246772A1 (en) 2022-03-29 2024-01-18 Illumina Inc. Chromenoquinoline dyes and uses in sequencing
US20230313294A1 (en) 2022-03-30 2023-10-05 Illumina Cambridge Limited Methods for chemical cleavage of surface-bound polynucleotides
EP4499870A1 (de) 2022-03-31 2025-02-05 Illumina, Inc. Zusammensetzungen und verfahren zur verbesserung von sequenzierungssignalen
AU2023244351A1 (en) 2022-03-31 2024-01-18 Illumina, Inc. Nucleosides and nucleotides with 3' vinyl blocking group useful in sequencing by synthesis
AU2023281871A1 (en) 2022-05-31 2024-11-14 Illumina, Inc Compositions and methods for nucleic acid sequencing
CA3259955A1 (en) 2022-06-28 2024-01-04 Illumina, Inc. FLUORESCENT DYES CONTAINING A FUSIONED BIS-TETRACYCLIC BORON HETEROCYCLE AND THEIR USES IN SEQUENCED
EP4570918A1 (de) 2022-08-09 2025-06-18 BGI Shenzhen Nachweissystem für dual-luciferase-reporter-gen und verwendung davon
US20250361558A1 (en) 2022-08-19 2025-11-27 Illumina, Inc. Third dna base pair site-specific dna detection
WO2024050815A1 (zh) 2022-09-09 2024-03-14 深圳华大生命科学研究院 杂芳环化合物在核酸检测中的用途
CN119213083A (zh) 2022-09-30 2024-12-27 伊路米纳有限公司 用于减少测序期间的光损伤的组合物和方法
EP4630435A1 (de) 2022-12-09 2025-10-15 Illumina, Inc. Nukleoside und nukleotide mit 3 ?-blockierungsgruppen und spaltbaren linkern
CA3246536A1 (en) 2022-12-22 2024-06-27 Illumina, Inc. TRANSITION METAL CATALYST COMPOSITIONS AND SYNTHETIC SEQUENCE PROCESSES
AU2023409219A1 (en) 2022-12-22 2024-10-03 Illumina, Inc. Palladium catalyst compositions and methods for sequencing by synthesis
CN119095984A (zh) 2022-12-27 2024-12-06 伊路米纳有限公司 使用3'烯丙基封端的核苷酸的测序方法
GB202300442D0 (en) * 2023-01-12 2023-03-01 Smi Drug Discovery Ltd Detecting and analysing analytes
AU2024247164A1 (en) 2023-03-29 2024-12-12 Illumina, Inc. Naphthalimide dyes and uses in nucleic acid sequencing
EP4689181A1 (de) 2023-03-30 2026-02-11 Illumina, Inc. Zusammensetzungen und verfahren zur nukleinsäuresequenzierung
WO2024216163A1 (en) 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using predominantly unlabeled nucleotides
WO2024216159A1 (en) 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using labeled nucleotides
WO2024240051A1 (zh) 2023-05-19 2024-11-28 深圳华大智造科技股份有限公司 一种荧光染料、其合成及其应用
WO2024243873A1 (zh) 2023-05-31 2024-12-05 深圳华大智造科技股份有限公司 一种调整核酸纳米球体积的方法及其应用
WO2025034461A1 (en) 2023-08-04 2025-02-13 Pacific Biosciences Of California, Inc. Detection of 5-methylcytosine
US20250075269A1 (en) 2023-08-31 2025-03-06 Illumina, Inc. Compositions and methods for nucleic acid sequencing
WO2025090596A1 (en) 2023-10-26 2025-05-01 Illumina, Inc. 4,5-substituted naphthalimide dyes and uses in nucleic acid sequencing
US20250163505A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Conjugated polymers or polymer dots as fluorescent labels
WO2025136890A1 (en) 2023-12-18 2025-06-26 Illumina, Inc. Hydrogel nanoparticles as labeling scaffold in sequencing
US20250215493A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Nucleotides with enzymatically cleavable 3'-o-glycoside blocking groups for sequencing
WO2025144711A1 (en) 2023-12-29 2025-07-03 Illumina, Inc. Tricyclic polymethine dyes for nucleic acid sequencing
WO2025184226A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. Nucleotides with terminal phosphate capping
WO2025188906A1 (en) 2024-03-08 2025-09-12 Illumina, Inc. Modified adenosine nucleotides
WO2025221895A1 (en) 2024-04-19 2025-10-23 Illumina, Inc. Water soluble polymer scaffolds for dye labeling
WO2025230914A1 (en) 2024-04-29 2025-11-06 Illumina, Inc. Nucleotides with enzyme-triggered self-immolative linkers for sequencing by synthesis
WO2025235623A1 (en) 2024-05-08 2025-11-13 Esbiolab, Llc Methods and systems for nucleic acid sequencing using an error-buffered coding scheme
WO2026039088A2 (en) 2024-06-03 2026-02-19 Esbiolab, Llc Excitation and detection methods for analyzing biomolecules

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US4979824A (en) * 1989-05-26 1990-12-25 Board Of Trustees Of The Leland Stanford Junior University High sensitivity fluorescent single particle and single molecule detection apparatus and method
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5314829A (en) * 1992-12-18 1994-05-24 California Institute Of Technology Method for imaging informational biological molecules on a semiconductor substrate
JPH07203998A (ja) * 1994-01-26 1995-08-08 Hamamatsu Photonics Kk 核酸塩基配列決定方法
FR2716263B1 (fr) * 1994-02-11 1997-01-17 Pasteur Institut Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon.
AU5171696A (en) * 1995-02-27 1996-09-18 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
US5851769A (en) * 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
US5780231A (en) * 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
DE19612356B4 (de) * 1996-03-28 2007-04-26 Clondiag Chip Technologies Gmbh Optischer Nachweis von Hybridisierungs-Signalen
ATE204290T1 (de) * 1996-11-06 2001-09-15 Sequenom Inc Zusammensetzungen und verfahren zur immobilisierung von nukleinsäure auf festträgern
US5837466A (en) * 1996-12-16 1998-11-17 Vysis, Inc. Devices and methods for detecting nucleic acid analytes in samples
JP4663824B2 (ja) * 1996-12-31 2011-04-06 ハイ スループット ジェノミクス インコーポレイテッド 多重化分子分析装置および方法
US6787308B2 (en) * 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing

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DE69928265D1 (de) 2005-12-15
AU770831B2 (en) 2004-03-04
US20050042649A1 (en) 2005-02-24
IL141148A0 (en) 2002-02-10
DE69928265T2 (de) 2006-07-20
CA2339121A1 (en) 2000-02-10
IS5831A (is) 2001-01-29
EP1105529A1 (de) 2001-06-13
AU5178799A (en) 2000-02-21
EP1105529B2 (de) 2013-05-29
EP1105529B1 (de) 2005-11-09
DE69928265T3 (de) 2013-11-28

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