ATE310815T1 - Chromosomale mutagenese in pichia methanolica - Google Patents

Chromosomale mutagenese in pichia methanolica

Info

Publication number
ATE310815T1
ATE310815T1 AT98947108T AT98947108T ATE310815T1 AT E310815 T1 ATE310815 T1 AT E310815T1 AT 98947108 T AT98947108 T AT 98947108T AT 98947108 T AT98947108 T AT 98947108T AT E310815 T1 ATE310815 T1 AT E310815T1
Authority
AT
Austria
Prior art keywords
cells
identified
adenine
altered
locus
Prior art date
Application number
AT98947108T
Other languages
English (en)
Inventor
Christopher K Raymond
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/001,141 external-priority patent/US6183953B1/en
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Application granted granted Critical
Publication of ATE310815T1 publication Critical patent/ATE310815T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
AT98947108T 1997-09-15 1998-09-11 Chromosomale mutagenese in pichia methanolica ATE310815T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US92974897A 1997-09-15 1997-09-15
US09/001,141 US6183953B1 (en) 1997-09-15 1997-12-30 Chromosomal mutagenesis in Pichia methanolica
PCT/US1998/019448 WO1999014320A1 (en) 1997-09-15 1998-09-11 CHROMOSOMAL MUTAGENESIS IN $i(PICHIA METHANOLICA)

Publications (1)

Publication Number Publication Date
ATE310815T1 true ATE310815T1 (de) 2005-12-15

Family

ID=26668622

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98947108T ATE310815T1 (de) 1997-09-15 1998-09-11 Chromosomale mutagenese in pichia methanolica

Country Status (8)

Country Link
EP (1) EP1015577B8 (de)
JP (1) JP2001516577A (de)
AT (1) ATE310815T1 (de)
AU (1) AU752578B2 (de)
CA (1) CA2303408C (de)
DE (1) DE69832504T2 (de)
DK (1) DK1015577T3 (de)
WO (1) WO1999014320A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6348331B1 (en) 1999-09-08 2002-02-19 Zymogenetics, Inc. Pichia methanolica glyceraldehyde-3-phosphate dehydrogenase 2 promoter
EP1210412A1 (de) * 1999-09-08 2002-06-05 ZymoGenetics, Inc. Pichia methanolica glyceraldehyd-3-phosphat-dehydrogenase 2 promotor und terminator

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE220105T1 (de) * 1991-04-01 2002-07-15 Merck & Co Inc Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
US5965389A (en) * 1995-11-09 1999-10-12 Zymogenetics, Inc. Production of GAD65 in methylotrophic yeast

Also Published As

Publication number Publication date
CA2303408C (en) 2005-05-03
DK1015577T3 (da) 2006-04-03
CA2303408A1 (en) 1999-03-25
DE69832504D1 (de) 2005-12-29
JP2001516577A (ja) 2001-10-02
EP1015577A1 (de) 2000-07-05
WO1999014320A1 (en) 1999-03-25
EP1015577B8 (de) 2006-10-04
AU752578B2 (en) 2002-09-26
AU9396498A (en) 1999-04-05
DE69832504T2 (de) 2006-08-17
EP1015577B1 (de) 2005-11-23

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Legal Events

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