ATE414782T1 - Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen - Google Patents

Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen

Info

Publication number
ATE414782T1
ATE414782T1 AT99960956T AT99960956T ATE414782T1 AT E414782 T1 ATE414782 T1 AT E414782T1 AT 99960956 T AT99960956 T AT 99960956T AT 99960956 T AT99960956 T AT 99960956T AT E414782 T1 ATE414782 T1 AT E414782T1
Authority
AT
Austria
Prior art keywords
mutant
polypeptide
cells
aspergillus cells
producing polypeptides
Prior art date
Application number
AT99960956T
Other languages
English (en)
Inventor
Bjorn Eggert Christensen
Henrik Moellgaard
Svend Kaasgaard
Jan Lehmbeck
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE414782T1 publication Critical patent/ATE414782T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungi isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/69—Aspergillus oryzae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT99960956T 1998-12-23 1999-12-22 Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen ATE414782T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199801726 1998-12-23
DKPA199900745 1999-05-27

Publications (1)

Publication Number Publication Date
ATE414782T1 true ATE414782T1 (de) 2008-12-15

Family

ID=26064565

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99960956T ATE414782T1 (de) 1998-12-23 1999-12-22 Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen

Country Status (9)

Country Link
EP (1) EP1141371B1 (de)
JP (2) JP2002533133A (de)
CN (1) CN100529096C (de)
AT (1) ATE414782T1 (de)
AU (1) AU1774000A (de)
DE (1) DE69939947D1 (de)
DK (1) DK1141371T3 (de)
ES (1) ES2317706T3 (de)
WO (1) WO2000039322A1 (de)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070957A2 (en) * 2002-02-20 2003-08-28 Novozymes A/S Plant polypeptide production
ATE372059T1 (de) 2002-10-11 2007-09-15 Novozymes As Verfahren zur herstellung von wärmebehandelten produkten
WO2004090549A1 (en) * 2003-04-11 2004-10-21 Novozymes A/S Method of screening for secreted glycosylated polypeptides
AU2004234472B2 (en) 2003-04-28 2009-01-08 Novozymes A/S Phospholipase and method of producing it
WO2005040372A1 (en) 2003-10-23 2005-05-06 Novozymes A/S Protease with improved stability in detergents
WO2006053565A2 (en) 2004-11-19 2006-05-26 Novozymes A/S Polypeptides having antimicrobial activity and polynucleotides encoding same
ES2543131T3 (es) 2004-12-22 2015-08-14 Novozymes North America, Inc. Enzimas para tratamiento de almidón
AU2006207463B2 (en) 2005-01-24 2011-03-17 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
EP1739428A1 (de) * 2005-07-01 2007-01-03 Institut Pasteur Diagnostic-Kit und Verfahren für Aspergillose
CN101292024B (zh) 2005-10-17 2012-04-18 诺维信公司 用于表达抗体的真菌突变体的用途
ES2534282T3 (es) 2006-06-29 2015-04-21 Dsm Ip Assets B.V. Un método para lograr la expresión polipeptídica mejorada
US8586330B2 (en) 2006-07-14 2013-11-19 Novozymes A/S Methods for producing secreted polypeptides having biological activity
JP5410981B2 (ja) * 2007-10-19 2014-02-05 公益財団法人野田産業科学研究所 ポリケタイドシンターゼ・ノンリボゾーマルペプチドシンテターゼ遺伝子
ES2667812T3 (es) 2008-11-20 2018-05-14 Novozymes Inc. Polipéptidos que tienen actividad potenciadora amilolítica y polinucleótidos que codifican los mismos
AU2010206181B2 (en) 2009-01-21 2015-01-15 Novozymes A/S Polypeptides having esterase activity and nucleic acids encoding the same
EP2411511B1 (de) 2009-03-24 2018-08-08 Novozymes A/S Polypeptide mit acetylxylan-esterase-akitivität und dafür codierende polynukleotide
EP2421986A1 (de) 2009-04-22 2012-02-29 DSM IP Assets B.V. Verfahren zur herstellung eines interessierenden rekombinanten polypeptids
BRPI1015536A2 (pt) 2009-04-24 2019-07-02 Dsm Ip Assets Bv polipeptídeo de degradação de carboidratos e usos do mesmo
EP3293264A1 (de) 2009-07-22 2018-03-14 DSM IP Assets B.V. Verbesserte wirtszelle für die herstellung von relevanten produkten
CN102666846A (zh) 2009-09-30 2012-09-12 诺维信公司 具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸
EP2496693B1 (de) 2009-11-06 2017-10-25 Novozymes, Inc. Polypeptide mit cellobiohydrolaseaktivität und dafür kodierende polynukleotide
EP2496692B1 (de) 2009-11-06 2016-03-16 Novozymes, Inc. Polypeptide mit xylanaseaktivität und für diese kodierende polynukleotide
MX2012005152A (es) 2009-11-06 2012-06-12 Novozymes Inc Composiciones para sacrificacion de material celulosico.
EA201201125A1 (ru) 2010-02-11 2013-03-29 ДСМ АйПи АССЕТС Б.В. Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала
US9133448B2 (en) 2010-02-11 2015-09-15 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
EP2585591B1 (de) 2010-06-25 2014-12-24 Novozymes A/S Polynukleotide mit Leitsequenzfunktion
WO2011161208A1 (en) 2010-06-25 2011-12-29 Novozymes A/S Polynucleotides having promoter activity
WO2011161207A1 (en) 2010-06-25 2011-12-29 Novozymes A/S Polynucleotides having promoter activity
WO2012000891A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
US9175050B2 (en) 2010-06-29 2015-11-03 Dsm Ip Assets B.V. Polypeptide having swollenin activity and uses thereof
BR112012033404B1 (pt) 2010-06-29 2021-05-18 Dsm Ip Assets B.V. célula recombinante, método para a preparação de um polipeptídeo, composição, método para o tratamento de um substrato que compreende material de carboidrato e uso de um polipeptídeo
DK2588494T3 (en) 2010-06-29 2018-06-14 Dsm Ip Assets Bv POLYPEPTIDE WITH BETA-GLUCOSIDASE ACTIVITY AND APPLICATIONS THEREOF
CA2803513A1 (en) 2010-06-29 2012-01-05 Margot Elisabeth Francoise Schooneveld-Bergmans Polypeptide having acetyl xylan esterase activity and uses thereof
CA2802128A1 (en) 2010-06-29 2012-01-05 Margot Elisabeth Francoise Schooneveld-Bergmans Polypeptide having beta-glucosidase activity and uses thereof
DK2588604T3 (en) 2010-06-30 2016-09-26 Novozymes Inc Polypeptides having beta-glucosidase activity and polynucleotides encoding them
CA2803222A1 (en) * 2010-07-01 2012-01-05 Dsm Ip Assets B.V. A method for the production of a compound of interest
US9273335B2 (en) 2010-08-12 2016-03-01 Novozymes, Inc. Compositions comprising a polypeptide having cellulolytic enhancing activity and a quinone compound and uses thereof
WO2012030811A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012030844A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
US9139823B2 (en) 2010-11-12 2015-09-22 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
CN103534348B (zh) 2011-01-26 2018-04-10 诺维信公司 具有纤维二糖水解酶活性的多肽和编码该多肽的多核苷酸
MX337913B (es) 2011-01-26 2016-03-28 Novozymes As Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican los mismos.
MX2013007720A (es) 2011-01-26 2013-08-09 Novozymes As Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican para los mismos.
US9080161B2 (en) 2011-01-26 2015-07-14 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012103322A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
EP2683830A1 (de) 2011-03-09 2014-01-15 Novozymes A/S Verfahren zur verstärkung der zellulolyse-verstärkenden aktivität eines polypeptids
US9926547B2 (en) 2011-04-28 2018-03-27 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
CN103797126A (zh) 2011-04-29 2014-05-14 诺维信股份有限公司 用于增强纤维素材料的降解或转化的方法
EP2527448A1 (de) 2011-05-23 2012-11-28 Novozymes A/S Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze
CN102321700A (zh) * 2011-08-03 2012-01-18 无锡赛德生物工程有限公司 一种曲酸发酵工艺及发酵设备
CA2846391A1 (en) 2011-08-24 2013-02-28 Novozymes, Inc. Aspergillus fumigatus cellulolytic enzyme compositions and uses thereof
DK2773656T3 (da) 2011-10-31 2019-09-09 Novozymes Inc Polypeptider med cellulolyseforbedrende aktivitet og polynukleotider, som koder for dem
EP3597736A1 (de) 2011-11-21 2020-01-22 Novozymes A/S Gh61-polypepvarianten und dafür codierende polynukleotide
CN119193513A (zh) 2012-04-27 2024-12-27 诺维信股份有限公司 Gh61多肽变体以及编码其的多核苷酸
AU2013311668B2 (en) 2012-09-05 2019-02-28 Novozymes A/S Polypeptides having protease activity
EP2898068A2 (de) 2012-09-19 2015-07-29 Novozymes, Inc. Verfahren zur verstärkung des verfalls oder der umwandlung von cellulose-material
US10035996B2 (en) 2012-10-08 2018-07-31 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US20150275194A1 (en) 2012-10-24 2015-10-01 Novozymes A/S Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
WO2014093835A1 (en) 2012-12-14 2014-06-19 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN110628749A (zh) 2013-03-08 2019-12-31 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
DK2994529T3 (en) 2013-05-10 2019-03-04 Novozymes As Polypeptides with xylanase activity and polynucleotides encoding them
EP3594335B1 (de) 2014-09-05 2024-05-01 Novozymes A/S Kohlenhydratbindende modulvarianten und polynukleotide zur codierung davon
DK3234143T5 (da) 2014-12-19 2024-10-14 Novozymes As Sammensætninger omfattende polypeptider med xylanaseaktivitet og polypeptider med arabinofuranosidaseaktivitet
DK3262165T3 (da) 2015-02-24 2020-08-24 Novozymes As Cellobiohydrolasevarianter og polynukleotider, som koder for dem
EP3303578B1 (de) 2015-05-27 2020-07-08 Novozymes A/S Polypeptide mit cellobiohydrolaseaktivität und dafür codierende polynukleotide
WO2017050242A1 (en) 2015-09-22 2017-03-30 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2018226171A2 (en) * 2017-06-07 2018-12-13 Ptt Global Chemical Public Company Limited Mutant strain aspergillus aculeatus for producing cellulase and xylanase and preparation method thereof
AU2018319349B2 (en) 2017-08-25 2024-02-29 Novozymes A/S Enzyme assisted crude palm oil extraction
DK3775222T5 (da) 2018-03-26 2024-08-19 Novozymes As Chaperoneproteiner fra svampe
CN108795903B (zh) * 2018-06-08 2020-12-15 南京林业大学 一种高酶活淀粉酶的表达方法
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CN108823178B (zh) * 2018-06-08 2021-12-10 中国中医科学院中药研究所 大黄素糖基转移酶蛋白FtUGT73BE5及其编码基因与应用
US12385027B2 (en) 2018-12-12 2025-08-12 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
EP3898985B1 (de) 2018-12-21 2023-02-08 Novozymes A/S Tandemproteinexpression
CN110218750B (zh) * 2019-04-24 2023-04-11 郑州轻工业学院 杂色曲霉菌株在发酵生产曲酸中应用
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EP4004211A1 (de) 2019-07-25 2022-06-01 Novozymes A/S Fadenpilzexpressionssystem
CN111139189B (zh) * 2020-01-14 2022-04-19 浙江工业大学 曲霉wbx-38及其在生产环匹阿尼酸中的应用
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WO2024133495A1 (en) 2022-12-21 2024-06-27 Novozymes A/S Microbial proteases for cell detachment
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Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1139457A (zh) * 1993-12-01 1997-01-01 诺沃诺尔迪斯克生物技术有限公司 曲霉表达系统
DE730656T1 (de) * 1993-12-01 1998-04-30 Novo Nordisk Biotech Inc Aspergillus poetidus exprimierungssystem
EP0966521A1 (de) * 1996-09-13 1999-12-29 Novo Nordisk Biotech, Inc. Zellen mit dna-insertionsmutationenwelche veränderte mengen eines polypeptids produzieren
AU4200897A (en) * 1996-09-19 1998-04-14 Novo Nordisk A/S Novel host cells and methods of producing proteins

Also Published As

Publication number Publication date
EP1141371A1 (de) 2001-10-10
WO2000039322A1 (en) 2000-07-06
JP2011188863A (ja) 2011-09-29
JP5453343B2 (ja) 2014-03-26
AU1774000A (en) 2000-07-31
CN1333837A (zh) 2002-01-30
ES2317706T3 (es) 2009-04-16
DK1141371T3 (da) 2009-02-02
JP2002533133A (ja) 2002-10-08
DE69939947D1 (de) 2009-01-02
EP1141371B1 (de) 2008-11-19
CN100529096C (zh) 2009-08-19

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