ATE312841T1 - Verfahren zum entschützen von oligonukleotide - Google Patents

Verfahren zum entschützen von oligonukleotide

Info

Publication number: ATE312841T1
Authority: AT; Austria
Prior art keywords: reagents; deprotection; electron; withdrawing group; active methylene
Prior art date: 2001-03-08

Application number

AT02750570T

Other languages

English (en)

Inventor

Jeffrey Nelson

Original Assignee

Applera Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-03-08

Filing date

2002-03-04

Publication date

2005-12-15

2002-03-04 Application filed by Applera Corp filed Critical Applera Corp

2005-12-15 Application granted granted Critical

2005-12-15 Publication of ATE312841T1 publication Critical patent/ATE312841T1/de

Links

108091034117 Oligonucleotide Proteins 0.000 title abstract 5
238000000034 method Methods 0.000 title abstract 5
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 3
239000003153 chemical reaction reagent Substances 0.000 abstract 7
238000010511 deprotection reaction Methods 0.000 abstract 4
125000006575 electron-withdrawing group Chemical group 0.000 abstract 2
-1 methylene compound Chemical class 0.000 abstract 2
125000004400 (C1-C12) alkyl group Chemical group 0.000 abstract 1
125000006736 (C6-C20) aryl group Chemical group 0.000 abstract 1
150000001412 amines Chemical class 0.000 abstract 1
125000000623 heterocyclic group Chemical group 0.000 abstract 1
229910052739 hydrogen Inorganic materials 0.000 abstract 1
239000001257 hydrogen Substances 0.000 abstract 1
125000004435 hydrogen atom Chemical group [H]* 0.000 abstract 1
239000012535 impurity Substances 0.000 abstract 1
125000006239 protecting group Chemical group 0.000 abstract 1
238000007086 side reaction Methods 0.000 abstract 1
125000001424 substituent group Chemical group 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Saccharide Compounds (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

AT02750570T 2001-03-08 2002-03-04 Verfahren zum entschützen von oligonukleotide ATE312841T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US27430901P	2001-03-08	2001-03-08
PCT/US2002/006739 WO2002072864A2 (en)	2001-03-08	2002-03-04	Reagents for oligonucleotide cleavage and deprotection

Publications (1)

Publication Number	Publication Date
ATE312841T1 true ATE312841T1 (de)	2005-12-15

Family

ID=23047660

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT02750570T ATE312841T1 (de)	2001-03-08	2002-03-04	Verfahren zum entschützen von oligonukleotide

Country Status (7)

Country	Link
US (2)	US6664388B2 (de)
EP (1)	EP1366057B1 (de)
JP (1)	JP2004521628A (de)
AT (1)	ATE312841T1 (de)
CA (1)	CA2468425A1 (de)
DE (1)	DE60207995T2 (de)
WO (1)	WO2002072864A2 (de)

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CA2721969C (en) *	2008-04-24	2016-06-07	Girindus America, Inc.	Process for the manufacture of oligonucleotides
WO2010025310A2 (en)	2008-08-27	2010-03-04	Westend Asset Clearinghouse Company, Llc	Methods and devices for high fidelity polynucleotide synthesis
WO2011056872A2 (en)	2009-11-03	2011-05-12	Gen9, Inc.	Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
EP2504449B1 (de)	2009-11-25	2016-03-23	Gen9, Inc.	Verfahren und vorrichtung für chipbasierte dna-fehlerreduktion
ES2574625T3 (es) *	2009-11-25	2016-06-21	Elitechgroup B.V.	Antagonistas de miARN de oligonucleótido conjugado con aglutinante del surco menor (MGB)
WO2011066185A1 (en)	2009-11-25	2011-06-03	Gen9, Inc.	Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en)	2010-01-07	2011-07-14	Gen9, Inc.	Assembly of high fidelity polynucleotides
CA2817697C (en)	2010-11-12	2021-11-16	Gen9, Inc.	Methods and devices for nucleic acids synthesis
WO2012064975A1 (en)	2010-11-12	2012-05-18	Gen9, Inc.	Protein arrays and methods of using and making the same
US9752176B2 (en)	2011-06-15	2017-09-05	Ginkgo Bioworks, Inc.	Methods for preparative in vitro cloning
JP2014526899A (ja)	2011-08-26	2014-10-09	ジェン９・インコーポレイテッド	核酸の高忠実度アセンブリのための組成物および方法
US9150853B2 (en)	2012-03-21	2015-10-06	Gen9, Inc.	Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en)	2012-04-24	2021-10-12	Gen9, Inc.	Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2014004393A1 (en)	2012-06-25	2014-01-03	Gen9, Inc.	Methods for nucleic acid assembly and high throughput sequencing
KR102160389B1 (ko)	2013-08-05	2020-09-28	트위스트 바이오사이언스 코포레이션	드 노보 합성된 유전자 라이브러리
CA2975852A1 (en)	2015-02-04	2016-08-11	Twist Bioscience Corporation	Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en)	2015-04-21	2016-10-27	Twist Bioscience Corporation	Devices and methods for oligonucleic acid library synthesis
KR20180050411A (ko)	2015-09-18	2018-05-14	트위스트 바이오사이언스 코포레이션	올리고핵산 변이체 라이브러리 및 그의 합성
WO2017053450A1 (en)	2015-09-22	2017-03-30	Twist Bioscience Corporation	Flexible substrates for nucleic acid synthesis
EP4664304A3 (de)	2016-09-21	2026-01-21	Atlas Data Storage, Inc.	Nukleinsäurebasierte datenspeicherung
US11634534B2 (en) *	2016-12-15	2023-04-25	The Government Of The United States Of America, As Represented By The Secretary Of The Navy	Degradable silyl-containing thermosetting networks
WO2018156792A1 (en)	2017-02-22	2018-08-30	Twist Bioscience Corporation	Nucleic acid based data storage
IL271205B2 (en)	2017-06-12	2025-02-01	Twist Bioscience Corp	Methods for assembling continuous nucleic acids
CN111565834B (zh) *	2017-10-20	2022-08-26	特韦斯特生物科学公司	用于多核苷酸合成的加热的纳米孔
CN109956987B (zh) *	2017-12-14	2022-05-17	南京金斯瑞生物科技有限公司	氨解方法及氨解组合物
EP3814497A4 (de)	2018-05-18	2022-03-02	Twist Bioscience Corporation	Polynukleotide, reagenzien und verfahren zur nukleinsäurehybridisierung
EP3902816A4 (de)	2018-12-26	2022-09-14	Twist Bioscience Corporation	Hochgenaue de-novo-polynukleotidsynthese
CA3131689A1 (en)	2019-02-26	2020-09-03	Twist Bioscience Corporation	Variant nucleic acid libraries for glp1 receptor
KR20210144698A (ko)	2019-02-26	2021-11-30	트위스트 바이오사이언스 코포레이션	항체 최적화를 위한 변이 핵산 라이브러리
CN115023440B (zh)	2019-09-23	2025-09-12	特韦斯特生物科学公司	结合CD3ε的抗体
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2002
- 2002-03-04 AT AT02750570T patent/ATE312841T1/de not_active IP Right Cessation
- 2002-03-04 EP EP02750570A patent/EP1366057B1/de not_active Expired - Lifetime
- 2002-03-04 JP JP2002571914A patent/JP2004521628A/ja active Pending
- 2002-03-04 US US10/091,231 patent/US6664388B2/en not_active Expired - Lifetime
- 2002-03-04 CA CA002468425A patent/CA2468425A1/en not_active Abandoned
- 2002-03-04 WO PCT/US2002/006739 patent/WO2002072864A2/en not_active Ceased
- 2002-03-04 DE DE60207995T patent/DE60207995T2/de not_active Expired - Fee Related
2003
- 2003-12-16 US US10/737,059 patent/US20040191808A1/en not_active Abandoned

Also Published As

Publication number	Publication date
EP1366057A2 (de)	2003-12-03
JP2004521628A (ja)	2004-07-22
US20040191808A1 (en)	2004-09-30
US6664388B2 (en)	2003-12-16
US20030181712A1 (en)	2003-09-25
DE60207995T2 (de)	2006-08-03
EP1366057B1 (de)	2005-12-14
EP1366057A4 (de)	2004-04-14
WO2002072864A3 (en)	2002-12-27
CA2468425A1 (en)	2003-09-19
DE60207995D1 (de)	2006-01-19
WO2002072864A2 (en)	2002-09-19

Legal Events

Date	Code	Title	Description
2006-06-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
DE60207995D1 (de)	2006-01-19	Verfahren zum entschützen von oligonukleotide
ES2620131T3 (es)	2017-06-27	Nucleótidos marcados
Hobartner et al.	2005	Modulation of RNA tertiary folding by incorporation of caged nucleotides
EP2526113B1 (de)	2016-08-10	Postsynthetische chemische modifikation von rna an der 2'-position des riboserings mittels klick-chemie
JP2024519372A5 (de)	2025-10-08
SG165995A1 (en)	2010-11-29	Phenol derivatives and their use as rotamase inhibitors
ATE359289T1 (de)	2007-05-15	Verbindungen zum schutz von hydroxylgruppen und ihre anwendung
ATE443071T1 (de)	2009-10-15	Verfahren zum entschützen von oligonukleotiden
RU2009128222A (ru)	2011-01-27	Система каталитического гидрохлорирования и способ получения винилхлорида из ацетилена и хлористого водорода в присутствии этой каталитической системы
EP0191659A1 (de)	1986-08-20	Adenosin-Nukleosid-Derivate, ihre Herstellung und biologische Verwendung
JPS61293553A (ja)	1986-12-24	新規な担体、担体の製造、新規な中間体生成物、担体のオリゴヌクレオチド合成への使用並びに担体に結合した新規なヌクレオシド及びオリゴヌクレオチド
JP2021510716A (ja)	2021-04-30	オリゴヌクレオチド及び核酸の合成
WO2008077600B1 (en)	2008-09-04	Compounds and methods for synthesis and purification of oligonucleotides
US9114104B2 (en)	2015-08-25	Proteinase K inhibitors, methods and compositions therefor
ATE277065T1 (de)	2004-10-15	Funktionalisierte verbindung, gegebenenfalls markierte polynukleotide und verfahren zur detektion einer ziel-nukleinsäure
US7723528B2 (en)	2010-05-25	Sulfur transfer reagents for oligonucleotide synthesis
GB2450661A (en)	2008-12-31	Compound, composition and use
RU2011141209A (ru)	2013-05-10	Усовершенствованный способ получения ингибиторов дипептидиллептизады-iv и промежуточного соединения
Schmidt et al.	2000	Solid‐Phase Synthesis of a Monofunctional trans‐a2PtII Complex Tethered to a Single‐Stranded Oligonucleotide
EA200601898A1 (ru)	2007-02-27	Простой способ синтеза соединений баккатина iii
Wu et al.	2008	An efficient one-pot synthesis of 3-substituted-5-amino-1, 2, 4-thiadiazoles from isothiocyanates and amidines
DE502005004209D1 (de)	2008-07-03	Verfahren zur reinigung und isolierung von nukleinsäuren unter verwendung kationischer detergentien
UA96411C2 (en)	2011-11-10	Synthesis of oligonucleotides
Akabane-Nakata et al.	2014	Synthesis of oligonucleotides containing N, N-disubstituted 3-deazacytosine nucleobases by post-elongation modification and their triplex-forming ability with double-stranded DNA
ES2312800T3 (es)	2009-03-01	Conversion de moleculas de taxano.