ATE312841T1 - Verfahren zum entschützen von oligonukleotide - Google Patents

Verfahren zum entschützen von oligonukleotide

Info

Publication number: ATE312841T1
Authority: AT; Austria
Prior art keywords: reagents; deprotection; electron; withdrawing group; active methylene
Prior art date: 2001-03-08

Application number

AT02750570T

Other languages

English (en)

Inventor

Jeffrey Nelson

Original Assignee

Applera Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-03-08

Filing date

2002-03-04

Publication date

2005-12-15

2002-03-04 Application filed by Applera Corp filed Critical Applera Corp

2005-12-15 Application granted granted Critical

2005-12-15 Publication of ATE312841T1 publication Critical patent/ATE312841T1/de

Links

108091034117 Oligonucleotide Proteins 0.000 title abstract 5
238000000034 method Methods 0.000 title abstract 5
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 3
239000003153 chemical reaction reagent Substances 0.000 abstract 7
238000010511 deprotection reaction Methods 0.000 abstract 4
125000006575 electron-withdrawing group Chemical group 0.000 abstract 2
-1 methylene compound Chemical class 0.000 abstract 2
125000004400 (C1-C12) alkyl group Chemical group 0.000 abstract 1
125000006736 (C6-C20) aryl group Chemical group 0.000 abstract 1
150000001412 amines Chemical class 0.000 abstract 1
125000000623 heterocyclic group Chemical group 0.000 abstract 1
229910052739 hydrogen Inorganic materials 0.000 abstract 1
239000001257 hydrogen Substances 0.000 abstract 1
125000004435 hydrogen atom Chemical group [H]* 0.000 abstract 1
239000012535 impurity Substances 0.000 abstract 1
125000006239 protecting group Chemical group 0.000 abstract 1
238000007086 side reaction Methods 0.000 abstract 1
125000001424 substituent group Chemical group 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Saccharide Compounds (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

AT02750570T 2001-03-08 2002-03-04 Verfahren zum entschützen von oligonukleotide ATE312841T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US27430901P	2001-03-08	2001-03-08
PCT/US2002/006739 WO2002072864A2 (en)	2001-03-08	2002-03-04	Reagents for oligonucleotide cleavage and deprotection

Publications (1)

Publication Number	Publication Date
ATE312841T1 true ATE312841T1 (de)	2005-12-15

Family

ID=23047660

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT02750570T ATE312841T1 (de)	2001-03-08	2002-03-04	Verfahren zum entschützen von oligonukleotide

Country Status (7)

Country	Link
US (2)	US6664388B2 (de)
EP (1)	EP1366057B1 (de)
JP (1)	JP2004521628A (de)
AT (1)	ATE312841T1 (de)
CA (1)	CA2468425A1 (de)
DE (1)	DE60207995T2 (de)
WO (1)	WO2002072864A2 (de)

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JP2007531794A (ja)	2004-04-05	2007-11-08	アルニラムファーマスーティカルズインコーポレイテッド	オリゴヌクレオチドの合成および精製に使用する方法および反応試薬
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US20080078535A1 (en) *	2006-10-03	2008-04-03	General Electric Company	Heat exchanger tube with enhanced heat transfer co-efficient and related method
CA2721969C (en) *	2008-04-24	2016-06-07	Girindus America, Inc.	Process for the manufacture of oligonucleotides
WO2010025310A2 (en)	2008-08-27	2010-03-04	Westend Asset Clearinghouse Company, Llc	Methods and devices for high fidelity polynucleotide synthesis
US10207240B2 (en)	2009-11-03	2019-02-19	Gen9, Inc.	Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
EP3597771A1 (de)	2009-11-25	2020-01-22	Gen9, Inc.	Verfahren und vorrichtung für chipbasierte dna-fehlerreduktion
US9334495B2 (en) *	2009-11-25	2016-05-10	Elitechgroup B.V.	Minor groove binder (MGB)-oligonucleotide miRNA antagonists
WO2011066185A1 (en)	2009-11-25	2011-06-03	Gen9, Inc.	Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en)	2010-01-07	2011-07-14	Gen9, Inc.	Assembly of high fidelity polynucleotides
WO2012064975A1 (en)	2010-11-12	2012-05-18	Gen9, Inc.	Protein arrays and methods of using and making the same
CN103502448B (zh)	2010-11-12	2017-03-29	Gen9股份有限公司	核酸合成的方法和设备
US9752176B2 (en)	2011-06-15	2017-09-05	Ginkgo Bioworks, Inc.	Methods for preparative in vitro cloning
LT2944693T (lt)	2011-08-26	2019-08-26	Gen9, Inc.	Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
US9150853B2 (en)	2012-03-21	2015-10-06	Gen9, Inc.	Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en)	2012-04-24	2021-10-12	Gen9, Inc.	Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
IL236303B (en)	2012-06-25	2022-07-01	Gen9 Inc	Methods for high-throughput nucleic acid assembly and sequencing
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US10669304B2 (en)	2015-02-04	2020-06-02	Twist Bioscience Corporation	Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en)	2015-04-21	2018-05-29	Twist Bioscience Corporation	Devices and methods for oligonucleic acid library synthesis
EP3350314A4 (de)	2015-09-18	2019-02-06	Twist Bioscience Corporation	Oligonukleinsäurevariantenbibliotheken variante und synthese davon
KR102794025B1 (ko)	2015-09-22	2025-04-09	트위스트 바이오사이언스 코포레이션	핵산 합성을 위한 가요성 기판
US10417457B2 (en)	2016-09-21	2019-09-17	Twist Bioscience Corporation	Nucleic acid based data storage
US11634534B2 (en) *	2016-12-15	2023-04-25	The Government Of The United States Of America, As Represented By The Secretary Of The Navy	Degradable silyl-containing thermosetting networks
EP4556433A3 (de)	2017-02-22	2025-08-06	Twist Bioscience Corporation	Nukleinsäurebasierte datenspeicherung
WO2018231872A1 (en)	2017-06-12	2018-12-20	Twist Bioscience Corporation	Methods for seamless nucleic acid assembly
CN111565834B (zh)	2017-10-20	2022-08-26	特韦斯特生物科学公司	用于多核苷酸合成的加热的纳米孔
CN109956987B (zh) *	2017-12-14	2022-05-17	南京金斯瑞生物科技有限公司	氨解方法及氨解组合物
CA3100739A1 (en)	2018-05-18	2019-11-21	Twist Bioscience Corporation	Polynucleotides, reagents, and methods for nucleic acid hybridization
WO2020139871A1 (en)	2018-12-26	2020-07-02	Twist Bioscience Corporation	Highly accurate de novo polynucleotide synthesis
JP2022522668A (ja)	2019-02-26	2022-04-20	ツイストバイオサイエンスコーポレーション	抗体を最適化するための変異体核酸ライブラリ
CN113766930B (zh)	2019-02-26	2025-07-22	特韦斯特生物科学公司	Glp1受体的变异核酸文库
JP2022548783A (ja)	2019-09-23	2022-11-21	ツイストバイオサイエンスコーポレーション	単一ドメイン抗体のバリアント核酸ライブラリー
EP4034566A4 (de)	2019-09-23	2024-01-24	Twist Bioscience Corporation	Variante nukleinsäurebibliotheken für crth2
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2002
- 2002-03-04 AT AT02750570T patent/ATE312841T1/de not_active IP Right Cessation
- 2002-03-04 CA CA002468425A patent/CA2468425A1/en not_active Abandoned
- 2002-03-04 US US10/091,231 patent/US6664388B2/en not_active Expired - Lifetime
- 2002-03-04 EP EP02750570A patent/EP1366057B1/de not_active Expired - Lifetime
- 2002-03-04 WO PCT/US2002/006739 patent/WO2002072864A2/en not_active Ceased
- 2002-03-04 DE DE60207995T patent/DE60207995T2/de not_active Expired - Fee Related
- 2002-03-04 JP JP2002571914A patent/JP2004521628A/ja active Pending
2003
- 2003-12-16 US US10/737,059 patent/US20040191808A1/en not_active Abandoned

Also Published As

Publication number	Publication date
US20030181712A1 (en)	2003-09-25
DE60207995T2 (de)	2006-08-03
US20040191808A1 (en)	2004-09-30
DE60207995D1 (de)	2006-01-19
EP1366057B1 (de)	2005-12-14
CA2468425A1 (en)	2003-09-19
JP2004521628A (ja)	2004-07-22
EP1366057A4 (de)	2004-04-14
US6664388B2 (en)	2003-12-16
WO2002072864A2 (en)	2002-09-19
EP1366057A2 (de)	2003-12-03
WO2002072864A3 (en)	2002-12-27

Legal Events

Date	Code	Title	Description
2006-06-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
ATE312841T1 (de)	2005-12-15	Verfahren zum entschützen von oligonukleotide
ES2620131T3 (es)	2017-06-27	Nucleótidos marcados
Pradeepkumar et al.	2008	DNA-catalyzed formation of nucleopeptide linkages
Hobartner et al.	2005	Modulation of RNA tertiary folding by incorporation of caged nucleotides
EP2526113B1 (de)	2016-08-10	Postsynthetische chemische modifikation von rna an der 2'-position des riboserings mittels klick-chemie
EP0191659B1 (de)	1990-04-11	Adenosin-Nukleosid-Derivate, ihre Herstellung und biologische Verwendung
JP2024519372A5 (de)	2025-10-08
SG165995A1 (en)	2010-11-29	Phenol derivatives and their use as rotamase inhibitors
ATE359289T1 (de)	2007-05-15	Verbindungen zum schutz von hydroxylgruppen und ihre anwendung
ATE443071T1 (de)	2009-10-15	Verfahren zum entschützen von oligonukleotiden
RU2009128222A (ru)	2011-01-27	Система каталитического гидрохлорирования и способ получения винилхлорида из ацетилена и хлористого водорода в присутствии этой каталитической системы
JPS61293553A (ja)	1986-12-24	新規な担体、担体の製造、新規な中間体生成物、担体のオリゴヌクレオチド合成への使用並びに担体に結合した新規なヌクレオシド及びオリゴヌクレオチド
WO2008077600B1 (en)	2008-09-04	Compounds and methods for synthesis and purification of oligonucleotides
US9114104B2 (en)	2015-08-25	Proteinase K inhibitors, methods and compositions therefor
ATE277065T1 (de)	2004-10-15	Funktionalisierte verbindung, gegebenenfalls markierte polynukleotide und verfahren zur detektion einer ziel-nukleinsäure
GB2450661A (en)	2008-12-31	Compound, composition and use
Schmidt et al.	2000	Solid‐Phase Synthesis of a Monofunctional trans‐a2PtII Complex Tethered to a Single‐Stranded Oligonucleotide
ECSP066983A (es)	2006-12-29	Un método fácil para sintetizar compuestos de bacatina iii
UA96411C2 (en)	2011-11-10	Synthesis of oligonucleotides
DE502005004209D1 (de)	2008-07-03	Verfahren zur reinigung und isolierung von nukleinsäuren unter verwendung kationischer detergentien
Wu et al.	2008	An efficient one-pot synthesis of 3-substituted-5-amino-1, 2, 4-thiadiazoles from isothiocyanates and amidines
Akabane-Nakata et al.	2014	Synthesis of oligonucleotides containing N, N-disubstituted 3-deazacytosine nucleobases by post-elongation modification and their triplex-forming ability with double-stranded DNA
Park et al.	2001	A new inhibitor design strategy for carboxypeptidase A as exemplified by N-(2-chloroethyl)-N-methylphenylalanine
ATE127783T1 (de)	1995-09-15	Reagens für schnelle peptidsynthese.
KR950701641A (ko)	1995-04-28	리보뉴클레오티드 환원 효소 억제제의 제조방법(A process for the preparation of ribonucleotide reductase inhibitors)