ATE312932T1 - Zwillingsarginin translokation in bacillus - Google Patents

Zwillingsarginin translokation in bacillus

Info

Publication number
ATE312932T1
ATE312932T1 AT01973140T AT01973140T ATE312932T1 AT E312932 T1 ATE312932 T1 AT E312932T1 AT 01973140 T AT01973140 T AT 01973140T AT 01973140 T AT01973140 T AT 01973140T AT E312932 T1 ATE312932 T1 AT E312932T1
Authority
AT
Austria
Prior art keywords
bacillus
gram
host cell
protein
secretion
Prior art date
Application number
AT01973140T
Other languages
English (en)
Inventor
Jan Douwe Harm Jongbloed
Joerg Paul Mueller
Dijl Jan Maarten Van
Sierd Bron
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE312932T1 publication Critical patent/ATE312932T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Cereal-Derived Products (AREA)
AT01973140T 2000-09-18 2001-09-17 Zwillingsarginin translokation in bacillus ATE312932T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23361000P 2000-09-18 2000-09-18
PCT/US2001/029151 WO2002022667A2 (en) 2000-09-18 2001-09-17 Twin-arginine translocation in bacillus

Publications (1)

Publication Number Publication Date
ATE312932T1 true ATE312932T1 (de) 2005-12-15

Family

ID=22877967

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01973140T ATE312932T1 (de) 2000-09-18 2001-09-17 Zwillingsarginin translokation in bacillus

Country Status (7)

Country Link
US (4) US7316924B2 (de)
EP (1) EP1356060B1 (de)
AT (1) ATE312932T1 (de)
AU (1) AU2001292749A1 (de)
DE (1) DE60115958T2 (de)
DK (1) DK1356060T3 (de)
WO (1) WO2002022667A2 (de)

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US7655249B2 (en) * 1998-09-30 2010-02-02 Corixa Corporation Compositions and methods for WT1 specific immunotherapy
US7329410B1 (en) 1998-09-30 2008-02-12 Corixa Corporation Compositions and method for WT1 specific immunotherapy
US7901693B2 (en) * 1998-09-30 2011-03-08 Corixa Corporation Compositions and methods for WT1 specific immunotherapy
US7553494B2 (en) * 2001-08-24 2009-06-30 Corixa Corporation WT1 fusion proteins
WO2003040335A2 (en) * 2001-11-05 2003-05-15 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
US7419783B2 (en) 2001-11-05 2008-09-02 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
EP1487966B1 (de) * 2002-03-23 2006-12-13 Research Development Foundation Sekretion von proteinen mit mehreren disulfidbindungen in bakterien und verwendungen davon
DE10256669B4 (de) 2002-12-04 2006-03-09 Universitätsklinikum Charité an der Humboldt-Universität zu Berlin Technologietransferstelle Gemisch mindestens zweier Fusionsproteine sowie ihre Herstellung und Verwendung
JP4730302B2 (ja) * 2004-04-20 2011-07-20 味の素株式会社 タンパク質の製造法
US20070026012A1 (en) * 2005-08-01 2007-02-01 Cornell Research Foundation, Inc. Compositions and methods for monitoring and altering protein folding and solubility
EP2360473A1 (de) * 2005-08-22 2011-08-24 Cornell Research Foundation Zusammensetzungen und Verfahren zur Analyse von Proteininteraktionen
KR100742218B1 (ko) 2005-10-21 2007-07-24 학교법인 포항공과대학교 트윈 아르기닌 트랜스로케이션 분비경로를 가지는오가노포스포러스 하이드롤라제 분비서열을 이용한대장균에서의 목적 외래 단백질 분비
EP2426199A3 (de) 2006-10-20 2012-08-22 Danisco US Inc. Polyoloxidasen
EP2431470B1 (de) 2006-11-30 2017-11-29 Novozymes A/S Rekombinante wirtszellen der dnase-expression
US8722584B2 (en) * 2007-01-12 2014-05-13 Cornell University Genetic selection for protein folding and solubility in the bacterial periplasm
KR20100024943A (ko) * 2007-06-18 2010-03-08 다니스코 유에스 인크. 원핵생물에서의 단백질 생성을 위한 ｔａｔ 신호 펩타이드
DE102008025926A1 (de) 2008-05-29 2009-12-03 Henkel Ag & Co. Kgaa Sekretionsoptimierter Mikroorganismus
DE102008025791A1 (de) 2008-05-29 2009-12-03 Henkel Ag & Co. Kgaa Sekretionsoptimierter Mikroorganismus
SI3044320T1 (sl) * 2013-09-11 2020-07-31 Impossible Foods Inc. Sekrecija polipeptidov, ki vsebujejo hem
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
PT3294762T (pt) 2015-05-11 2022-03-21 Impossible Foods Inc Constructos de expressão e métodos de modificar geneticamente levedura metilotrófica
KR101791526B1 (ko) * 2016-02-18 2017-11-01 (주)케어젠 발모 촉진 활성을 나타내는 펩타이드 및 이의 용도
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
GB201713732D0 (en) * 2017-08-25 2017-10-11 Alta Innovations Ltd Tat expression system
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
EP3959311A1 (de) 2019-04-25 2022-03-02 Impossible Foods Inc. Stämme und verfahren zur herstellung von häm-haltigen proteinen
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
US12537071B1 (en) 2020-07-22 2026-01-27 David Gordon Bermudes Bacteria having boolean control pathways expressing therapeutic proteins including immunotherapeutic cytotoxins

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NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
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US5641671A (en) * 1990-07-06 1997-06-24 Unilever Patent Holdings B.V. Production of active Pseudomonas glumae lipase in homologous or heterologous hosts
WO1999051753A1 (en) * 1998-04-01 1999-10-14 The Governors Of The University Of Alberta Compositions and methods for protein secretion
DK1354056T3 (en) * 2000-10-10 2015-02-23 Danisco Us Inc IMPROVED secretion of a polypeptide by a microorganism

Also Published As

Publication number Publication date
EP1356060B1 (de) 2005-12-14
WO2002022667A3 (en) 2003-09-04
US20090093025A1 (en) 2009-04-09
US20080248525A1 (en) 2008-10-09
US7884192B2 (en) 2011-02-08
WO2002022667A2 (en) 2002-03-21
WO2002022667A9 (en) 2003-03-20
US20080166757A1 (en) 2008-07-10
DE60115958D1 (en) 2006-01-19
US7884191B2 (en) 2011-02-08
EP1356060A2 (de) 2003-10-29
DK1356060T3 (da) 2006-05-01
US7897742B2 (en) 2011-03-01
AU2001292749A1 (en) 2002-03-26
DE60115958T2 (de) 2006-08-03
US20020110860A1 (en) 2002-08-15
US7316924B2 (en) 2008-01-08

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