ATE313620T1 - Wirtszellen und methoden für die produktion von proteinen - Google Patents

Wirtszellen und methoden für die produktion von proteinen

Info

Publication number
ATE313620T1
ATE313620T1 AT97939990T AT97939990T ATE313620T1 AT E313620 T1 ATE313620 T1 AT E313620T1 AT 97939990 T AT97939990 T AT 97939990T AT 97939990 T AT97939990 T AT 97939990T AT E313620 T1 ATE313620 T1 AT E313620T1
Authority
AT
Austria
Prior art keywords
proteins
methods
host cell
relates
host cells
Prior art date
Application number
AT97939990T
Other languages
English (en)
Inventor
Jan Lehmbeck
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE313620T1 publication Critical patent/ATE313620T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT97939990T 1996-09-19 1997-09-19 Wirtszellen und methoden für die produktion von proteinen ATE313620T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK102496 1996-09-19
PCT/DK1997/000397 WO1998012300A1 (en) 1996-09-19 1997-09-19 Novel host cells and methods of producing proteins

Publications (1)

Publication Number Publication Date
ATE313620T1 true ATE313620T1 (de) 2006-01-15

Family

ID=8100132

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97939990T ATE313620T1 (de) 1996-09-19 1997-09-19 Wirtszellen und methoden für die produktion von proteinen

Country Status (10)

Country Link
US (1) US6352841B1 (de)
EP (1) EP0956338B1 (de)
JP (1) JP4091119B2 (de)
CN (1) CN1262639C (de)
AT (1) ATE313620T1 (de)
AU (1) AU4200897A (de)
DE (1) DE69734936T2 (de)
DK (1) DK0956338T3 (de)
ES (1) ES2256895T3 (de)
WO (1) WO1998012300A1 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU753578B2 (en) 1997-12-22 2002-10-24 Novozymes A/S Carbohydrate oxidase and use thereof in baking
US6806062B1 (en) 1998-10-05 2004-10-19 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
AU6188599A (en) 1998-10-26 2000-05-15 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
JP2002533133A (ja) * 1998-12-23 2002-10-08 ノボザイムス アクティーゼルスカブ アスペルギルス変異体細胞におけるポリペプチドの生産方法
ATE469223T1 (de) 2000-03-14 2010-06-15 Novozymes As Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
EP2119773A1 (de) 2000-06-26 2009-11-18 Novozymes A/S Lipolytische Enzyme aus Fusarium und Acremonium Stämmen
BR0113681A (pt) 2000-09-05 2003-07-08 Novozymes As Polipeptìdeo tendo atividade da enzima lipoxigenase, polinucleotìdeo, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir uma lipoxigenase, sonda de oligonucleotìdeo, método para obter um polipeptìdeo com atividade de lipoxigenase, composição de massa, métodos para preparar uma massa ou um produto assado produzido da massa, e para oxigenar um substrato, e, composição detergente
US7063962B2 (en) 2001-07-20 2006-06-20 Novozymes A/S DNA sequences for regulating transcription
AU2003229461A1 (en) * 2002-05-24 2003-12-12 Universite Laval Transformed fungi for production of recombinant proteins
US7713725B2 (en) * 2002-09-10 2010-05-11 Danisco Us Inc. Induction of gene expression using a high concentration sugar mixture
AU2004234472B2 (en) 2003-04-28 2009-01-08 Novozymes A/S Phospholipase and method of producing it
DE602004022967D1 (de) 2003-10-30 2009-10-15 Novozymes As Kohlenhydratbindende module
GB2426523B (en) 2004-03-01 2008-10-22 Kitakyushu Foundation Human cell strains for protein production,provided by selecting strains with high intracellular protein and mutating with carcinogens
GB0405659D0 (en) * 2004-03-12 2004-04-21 Horticulture Res Internat Altered expression in filamentous fungi
ES2543131T3 (es) 2004-12-22 2015-08-14 Novozymes North America, Inc. Enzimas para tratamiento de almidón
AU2006207463B2 (en) 2005-01-24 2011-03-17 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
US7696318B2 (en) 2005-07-13 2010-04-13 Novo Nordisk Health Care Ag Host cell protein knock-out cells for production of therapeutic proteins
WO2007015470A1 (ja) * 2005-08-03 2007-02-08 Asahi Glass Company, Limited 酵母宿主、形質転換体および異種タンパク質の製造方法
CN101292024B (zh) * 2005-10-17 2012-04-18 诺维信公司 用于表达抗体的真菌突变体的用途
CN101883848A (zh) 2007-10-01 2010-11-10 诺维信公司 具有蛋白酶活性的多肽和编码该多肽的多核苷酸
WO2010039840A1 (en) 2008-09-30 2010-04-08 Novozymes, Inc. Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum
EP3293264A1 (de) 2009-07-22 2018-03-14 DSM IP Assets B.V. Verbesserte wirtszelle für die herstellung von relevanten produkten
MX350399B (es) 2009-12-18 2017-09-06 Novozymes Inc Metodos para producir polipeptidos en mutantes de trichoderma deficientes en proteasa.
JP5836946B2 (ja) * 2010-06-17 2015-12-24 キッコーマン株式会社 麹菌染色体における大領域重複の作製方法
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
EP2800809B1 (de) 2012-01-05 2018-03-07 Glykos Finland Oy Filamentöse pilzzellen mit proteasemangel und verwendungsverfahren dafür
JP6081245B2 (ja) * 2012-04-07 2017-02-15 キッコーマン株式会社 麹菌染色体における任意領域の重複転座の作製方法
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
DK3019602T3 (en) 2013-07-10 2018-11-12 Glykos Finland Oy MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
DK3234143T5 (da) 2014-12-19 2024-10-14 Novozymes As Sammensætninger omfattende polypeptider med xylanaseaktivitet og polypeptider med arabinofuranosidaseaktivitet
TW201702380A (zh) * 2015-02-27 2017-01-16 再生元醫藥公司 宿主細胞蛋白質修飾
JP6245299B2 (ja) * 2016-04-27 2017-12-13 バクスアルタ ゲーエムベーハー 組換え安定細胞クローン、その産生およびその使用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5480794A (en) 1987-06-29 1996-01-02 Massachusetts Institute Of Technology And Metabolix, Inc. Overproduction and purification of soluble PHA synthase
GB8727772D0 (en) * 1987-11-27 1987-12-31 Celltech Ltd Host cells
ATE248925T1 (de) * 1988-01-05 2003-09-15 Roche Diagnostics Gmbh Verfahren zur herstellung von proteinen oder proteinhaltigen genprodukten
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
JPH06506117A (ja) * 1991-04-01 1994-07-14 メルク　エンド　カンパニー　インコーポレーテッド ピキア（Ｐｉｃｈｉａ）蛋白質分解活性に影響する遺伝子およびその使用
US5674728A (en) * 1993-11-03 1997-10-07 Novartis Corporation Aspergillus niger vacuolar aspartyl protease
US5589364A (en) 1994-07-29 1996-12-31 Magainin Pharmaceuticals Inc. Recombinant production of biologically active peptides and proteins
JP3974171B2 (ja) * 1995-03-20 2007-09-12 ノボザイムス アクティーゼルスカブ 低下したレベルのメタロプロテアーゼを発見する宿主細胞及びその細胞をタンパク質の生産に用いる方法
CN1120235C (zh) 1996-03-27 2003-09-03 诺沃奇梅兹有限公司 碱性蛋白酶缺陷型丝状真菌

Also Published As

Publication number Publication date
EP0956338B1 (de) 2005-12-21
AU4200897A (en) 1998-04-14
JP2001500381A (ja) 2001-01-16
WO1998012300A1 (en) 1998-03-26
EP0956338A1 (de) 1999-11-17
CN1262639C (zh) 2006-07-05
JP4091119B2 (ja) 2008-05-28
CN1230986A (zh) 1999-10-06
DE69734936D1 (de) 2006-01-26
ES2256895T3 (es) 2006-07-16
DK0956338T3 (da) 2006-05-08
US6352841B1 (en) 2002-03-05
DE69734936T2 (de) 2006-08-24

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