ATE318891T1 - Testverfahren zur bestimmung von gen-imprinting und methylierte cpg inseln - Google Patents

Testverfahren zur bestimmung von gen-imprinting und methylierte cpg inseln

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Publication number
ATE318891T1
ATE318891T1 AT01941519T AT01941519T ATE318891T1 AT E318891 T1 ATE318891 T1 AT E318891T1 AT 01941519 T AT01941519 T AT 01941519T AT 01941519 T AT01941519 T AT 01941519T AT E318891 T1 ATE318891 T1 AT E318891T1
Authority
AT
Austria
Prior art keywords
methylated cpg
cpg islands
test methods
determining gene
gene imprinting
Prior art date
Application number
AT01941519T
Other languages
English (en)
Inventor
Andrew Feinberg
Liora Strichman-Almashanu
Shan Jiang
Original Assignee
Univ Johns Hopkins
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Univ Johns Hopkins filed Critical Univ Johns Hopkins
Application granted granted Critical
Publication of ATE318891T1 publication Critical patent/ATE318891T1/de

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0611—Primordial germ cells, e.g. embryonic germ cells [EG]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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    • C12N2503/00—Use of cells in diagnostics
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    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
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    • C12N2510/00—Genetically modified cells
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Eye Examination Apparatus (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
AT01941519T 2000-05-22 2001-05-22 Testverfahren zur bestimmung von gen-imprinting und methylierte cpg inseln ATE318891T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20616100P 2000-05-22 2000-05-22
US20615800P 2000-05-22 2000-05-22

Publications (1)

Publication Number Publication Date
ATE318891T1 true ATE318891T1 (de) 2006-03-15

Family

ID=26901093

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01941519T ATE318891T1 (de) 2000-05-22 2001-05-22 Testverfahren zur bestimmung von gen-imprinting und methylierte cpg inseln

Country Status (8)

Country Link
US (4) US6960434B2 (de)
EP (1) EP1290139B1 (de)
JP (1) JP2003534005A (de)
AT (1) ATE318891T1 (de)
AU (2) AU7486601A (de)
CA (1) CA2410317A1 (de)
DE (1) DE60117542T2 (de)
WO (1) WO2001090313A2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001279046A1 (en) * 2000-07-28 2002-02-13 Millennium Pharmaceuticals, Inc. 18431 and 32374, human protein kinase family members and uses therefor
EP3098231A1 (de) * 2001-11-30 2016-11-30 The John Hopkins University Verfahren zur analyse methylierter cpg-inseln und gc-reicher bereiche
DK1342794T3 (da) * 2002-03-05 2006-04-24 Epigenomics Ag Fremgangsmåde og anordning til at bestemme vævsspecificitet af fritflydende DNA i legemsvæsker
US20060024676A1 (en) * 2003-04-14 2006-02-02 Karen Uhlmann Method of detecting epigenetic biomarkers by quantitative methyISNP analysis
FR2854903A1 (fr) * 2003-05-13 2004-11-19 Centre Nat Rech Scient Utilisation des loci bage (b melanoma antigens) comme marqueurs tumoraux
US20080102452A1 (en) * 2006-10-31 2008-05-01 Roberts Douglas N Control nucleic acid constructs for use in analysis of methylation status
US20090246771A1 (en) * 2007-11-02 2009-10-01 University Of Southern California Compositions and methods comprising biomarkers of sperm quality, semen quality and fertility
US20090137024A1 (en) * 2007-11-28 2009-05-28 Canon U.S. Life Sciences, Inc. Method of Separating Target DNA from Mixed DNA
US20110256538A1 (en) * 2010-04-15 2011-10-20 Philadelphia Health & Education Corporation D/B/A Drexel University College Of Medicine Epigenomic dna modifications for tissue typing, early cancer detection, and disease management
JP2013545439A (ja) 2010-09-17 2013-12-26 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 多能性幹細胞の有用性および安全性の特徴決定を行うための機能的ゲノミクスアッセイ
US8778638B2 (en) 2010-12-10 2014-07-15 The Board Of Trustees Of The Leland Stanford Junior University Phased genome sequencing
US11566284B2 (en) 2016-08-10 2023-01-31 Grail, Llc Methods of preparing dual-indexed DNA libraries for bisulfite conversion sequencing
WO2018214249A1 (zh) * 2017-05-22 2018-11-29 立森印迹诊断技术（无锡）有限公司 一种印记基因分级模型及其组成的系统和应用
CN110714075B (zh) * 2018-07-13 2024-05-03 立森印迹诊断技术（无锡）有限公司 一种用于检测肺肿瘤良恶性程度的分级模型及其应用
CN110791563B (zh) * 2018-08-01 2024-02-09 立森印迹诊断技术（无锡）有限公司 一种用于检测甲状腺肿瘤良恶性程度的分级模型及其应用
US20220262462A1 (en) * 2019-04-10 2022-08-18 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Computational filtering of methylated sequence data for predictive modeling
CN111613271B (zh) * 2020-04-26 2023-02-14 西南大学 一种预测畜禽数量性状显性遗传效应的方法及应用
KR20250008056A (ko) 2022-05-11 2025-01-14 프로빈셜 헬스 서비시즈 오쏘리티 유전성 질환의 진단 및 관리를 위한 부모 기원 질환 대립유전자 검출 방법 및 장치

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6617434B1 (en) * 1996-05-31 2003-09-09 North Shore Long Island Jewish Research Institute Identificiaton of differentially methylated and mutated nucleic acids
US6235474B1 (en) * 1996-12-30 2001-05-22 The Johns Hopkins University Methods and kits for diagnosing and determination of the predisposition for diseases
US6251594B1 (en) * 1997-06-09 2001-06-26 Usc/Norris Comprehensive Cancer Ctr. Cancer diagnostic method based upon DNA methylation differences
EP0976835B1 (de) * 1998-07-29 2003-07-09 Keygene N.V. Verfahren zur Erkennung von Nukleinsäuremethylierungen durch AFLP
US7700324B1 (en) 1998-11-03 2010-04-20 The Johns Hopkins University School Of Medicine Methylated CpG island amplification (MCA)

Also Published As

Publication number Publication date
DE60117542D1 (de) 2006-04-27
EP1290139B1 (de) 2006-03-01
EP1290139A2 (de) 2003-03-12
CA2410317A1 (en) 2001-11-29
US20020045257A1 (en) 2002-04-18
US6960434B2 (en) 2005-11-01
US7488815B2 (en) 2009-02-10
AU7486601A (en) 2001-12-03
JP2003534005A (ja) 2003-11-18
US20100273658A1 (en) 2010-10-28
AU2001274866B2 (en) 2006-11-09
WO2001090313A8 (en) 2003-11-13
US20050282147A1 (en) 2005-12-22
US7985550B2 (en) 2011-07-26
WO2001090313A2 (en) 2001-11-29
US20050153440A1 (en) 2005-07-14
WO2001090313A3 (en) 2002-05-16
WO2001090313A9 (en) 2003-03-06
DE60117542T2 (de) 2006-12-07

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