ATE244771T1 - Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp - Google Patents

Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp

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Publication number
ATE244771T1
ATE244771T1 AT98202549T AT98202549T ATE244771T1 AT E244771 T1 ATE244771 T1 AT E244771T1 AT 98202549 T AT98202549 T AT 98202549T AT 98202549 T AT98202549 T AT 98202549T AT E244771 T1 ATE244771 T1 AT E244771T1
Authority
AT
Austria
Prior art keywords
aflp
methylated
generating
dna
fingerprint
Prior art date
Application number
AT98202549T
Other languages
English (en)
Inventor
Marnik Johan Roger Vuylsteke
Petrus Antonius Josephina Vos
Marcus Florent Oscar Zabeau
Original Assignee
Keygene Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Keygene Nv filed Critical Keygene Nv
Application granted granted Critical
Publication of ATE244771T1 publication Critical patent/ATE244771T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT98202549T 1998-07-29 1998-07-29 Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp ATE244771T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP98202549A EP0976835B1 (de) 1998-07-29 1998-07-29 Verfahren zur Erkennung von Nukleinsäuremethylierungen durch AFLP

Publications (1)

Publication Number Publication Date
ATE244771T1 true ATE244771T1 (de) 2003-07-15

Family

ID=8233987

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98202549T ATE244771T1 (de) 1998-07-29 1998-07-29 Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp

Country Status (4)

Country Link
US (1) US6300071B1 (de)
EP (1) EP0976835B1 (de)
AT (1) ATE244771T1 (de)
DE (1) DE69816286T2 (de)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6605432B1 (en) 1999-02-05 2003-08-12 Curators Of The University Of Missouri High-throughput methods for detecting DNA methylation
CA2360929A1 (en) * 1999-02-05 2000-08-10 Amersham Pharmacia Biotech Uk Limited Genomic analysis method
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US6828098B2 (en) 2000-05-20 2004-12-07 The Regents Of The University Of Michigan Method of producing a DNA library using positional amplification based on the use of adaptors and nick translation
JP2003534005A (ja) * 2000-05-22 2003-11-18 ザ ジョンズ ホプキンス ユニバーシティ 遺伝子インプリンティングおよびメチル化ＣｐＧアイランドのアッセイ法
AU2002221800A1 (en) * 2000-11-02 2002-05-15 Epigenomics Ag Systems, methods and computer program products for guiding the selection of therapeutic treatment regimens
US20030143554A1 (en) * 2001-03-31 2003-07-31 Berres Mark E. Method of genotyping by determination of allele copy number
AU2002318336A1 (en) * 2001-06-08 2002-12-23 U.S. Genomics, Inc. Methods and products for analyzing nucleic acids based on methylation status
WO2003002752A2 (en) * 2001-06-29 2003-01-09 Rubicon Genomics Inc. Methods of using nick translate libraries for snp analysis
JP2006500901A (ja) * 2001-09-26 2006-01-12 エピジェンクス ファーマスーティカルズ、 インコーポレイテッド Ｄｎａメチル化変化のアッセイ
US20030232371A1 (en) * 2001-10-24 2003-12-18 Bestor Timothy H. Methods for detecting methylated promoters based on differential DNA methylation
US20030099997A1 (en) * 2001-10-24 2003-05-29 Bestor Timothy H. Method for gene identification based on differential DNA methylation
US20110151438A9 (en) * 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
AU2003212878A1 (en) * 2002-01-30 2003-09-02 Epigenomics Ag Identification of cell differentiation states based on methylation patterns
AU2003224978A1 (en) 2002-04-12 2003-10-27 Curators Of The University Of Missouri ECIST microarrays for dual screening of DNA hypermethylation and gene silencing
US20060094016A1 (en) * 2002-12-02 2006-05-04 Niall Gormley Determination of methylation of nucleic acid sequences
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
US20050032102A1 (en) * 2003-07-22 2005-02-10 Affymetrix, Inc. Mapping genomic rearrangements
US20050123944A1 (en) * 2003-08-01 2005-06-09 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
DE10353419B3 (de) * 2003-11-09 2005-06-30 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA-Sequenzen mittels hemimethylierungssensitiver Restriktionsenzyme
WO2005090607A1 (en) 2004-03-08 2005-09-29 Rubicon Genomics, Inc. Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation
US7364855B2 (en) 2004-04-30 2008-04-29 Applera Corporation Methods and kits for methylation detection
EP1632578A1 (de) 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
WO2006038416A1 (ja) * 2004-10-06 2006-04-13 National Institute Of Radiological Sciences ゲノム上の転写活性領域（非メチル化領域）の網羅的解析法
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
CN105039313B (zh) 2005-06-23 2018-10-23 科因股份有限公司 用于多态性的高通量鉴定和检测的策略
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US8409804B2 (en) 2005-08-02 2013-04-02 Rubicon Genomics, Inc. Isolation of CpG islands by thermal segregation and enzymatic selection-amplification method
ATE510930T1 (de) 2005-08-02 2011-06-15 Rubicon Genomics Inc Zusammensetzungen und verfahren zur bearbeitung und amplifikation von dna mit verwendung mehrerer enzyme in einer einzigen reaktion
CA2910861C (en) 2005-09-29 2018-08-07 Michael Josephus Theresia Van Eijk High throughput screening of mutagenized populations
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
EP1999271B1 (de) 2005-12-22 2013-07-03 Instytut Hodowli I Aklimatyzacji Roslin (Plant Breeding and Acclimatization Institute) Verfahren zur identifizierung induzierter variabilität in in-vitro-kulturen
JP5452021B2 (ja) 2005-12-22 2014-03-26 キージーン ナムローゼ フェンノートシャップ ハイスループットａｆｌｐ系多型検出法
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US20090253581A1 (en) 2006-04-04 2009-10-08 Keygene N.V. High Throughput Detection of Molecular Markers Based on AFLP and High Throughput Sequencing
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
EP2130927A4 (de) * 2007-03-07 2010-08-04 Univ Tokyo Verfahren zur amplifikation eines dna-fragments
US8642294B2 (en) * 2007-06-20 2014-02-04 Albert Einstein College Of Medicine Of Yeshiva University Methods for determining cytosine methylation in DNA and uses thereof
US8541207B2 (en) * 2008-10-22 2013-09-24 Illumina, Inc. Preservation of information related to genomic DNA methylation
WO2010080617A2 (en) 2008-12-19 2010-07-15 The Board Of Trustees Of The University Of Illinois Detecting and sorting methylated dna using a synthetic nanopore
US20100273164A1 (en) * 2009-03-24 2010-10-28 President And Fellows Of Harvard College Targeted and Whole-Genome Technologies to Profile DNA Cytosine Methylation
US8748091B2 (en) * 2009-12-18 2014-06-10 The Board Of Trustees Of The University Of Illinois Characterizing stretched polynucleotides in a synthetic nanopassage
US10435743B2 (en) 2011-05-20 2019-10-08 The Regents Of The University Of California Method to estimate age of individual based on epigenetic markers in biological sample
US10058306B2 (en) 2012-06-22 2018-08-28 Preprogen, LLC Method for obtaining fetal cells and fetal cellular components
EP3740589A4 (de) 2018-01-17 2021-11-03 The Regents of the University of California Auf phänotypischem alter und dna-methylierung basierte biomarker für lebenserwartung und morbidität
CA3126683A1 (en) 2019-01-18 2020-07-23 The Regents Of The University Of California Dna methylation measurement for mammals based on conserved loci
WO2023175019A1 (en) 2022-03-15 2023-09-21 Genknowme S.A. Method determining the difference between the biological age and the chronological age of a subject
WO2024256726A1 (en) 2023-06-15 2024-12-19 Genknowme S.A. Computer implemented method determining a value of allostatic load of a human being

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU223760B1 (hu) * 1991-09-24 2005-01-28 Keygene N.V. Szelektív restrikciós fragmentumsokszorosítás, általános módszer DNS-fingerprint analízisére
US5843649A (en) * 1995-03-01 1998-12-01 University Of Colorado Method of identifying clonal cell samples using heteroduplex generators
US5871917A (en) * 1996-05-31 1999-02-16 North Shore University Hospital Research Corp. Identification of differentially methylated and mutated nucleic acids
AU4174397A (en) * 1996-08-30 1998-03-19 Life Technologies, Inc. Methods for identification and isolation of specific nucleotide sequences in cdna and genomic dna

Also Published As

Publication number Publication date
EP0976835B1 (de) 2003-07-09
DE69816286D1 (de) 2003-08-14
DE69816286T2 (de) 2004-05-27
EP0976835A1 (de) 2000-02-02
US6300071B1 (en) 2001-10-09

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