ATE244771T1 - Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp - Google Patents

Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp

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Publication number
ATE244771T1
ATE244771T1 AT98202549T AT98202549T ATE244771T1 AT E244771 T1 ATE244771 T1 AT E244771T1 AT 98202549 T AT98202549 T AT 98202549T AT 98202549 T AT98202549 T AT 98202549T AT E244771 T1 ATE244771 T1 AT E244771T1
Authority
AT
Austria
Prior art keywords
aflp
methylated
generating
dna
fingerprint
Prior art date
Application number
AT98202549T
Other languages
English (en)
Inventor
Marnik Johan Roger Vuylsteke
Petrus Antonius Josephina Vos
Marcus Florent Oscar Zabeau
Original Assignee
Keygene Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Keygene Nv filed Critical Keygene Nv
Application granted granted Critical
Publication of ATE244771T1 publication Critical patent/ATE244771T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT98202549T 1998-07-29 1998-07-29 Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp ATE244771T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP98202549A EP0976835B1 (de) 1998-07-29 1998-07-29 Verfahren zur Erkennung von Nukleinsäuremethylierungen durch AFLP

Publications (1)

Publication Number Publication Date
ATE244771T1 true ATE244771T1 (de) 2003-07-15

Family

ID=8233987

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98202549T ATE244771T1 (de) 1998-07-29 1998-07-29 Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp

Country Status (4)

Country Link
US (1) US6300071B1 (de)
EP (1) EP0976835B1 (de)
AT (1) ATE244771T1 (de)
DE (1) DE69816286T2 (de)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6605432B1 (en) 1999-02-05 2003-08-12 Curators Of The University Of Missouri High-throughput methods for detecting DNA methylation
AU2308900A (en) * 1999-02-05 2000-08-25 Amersham Pharmacia Biotech Uk Limited Analysis method
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
EP1290225A4 (de) 2000-05-20 2004-09-15 Univ Michigan Methode zur herstellung einer dna-bank durch positionale vervielfältigung
AU7486601A (en) * 2000-05-22 2001-12-03 Univ Johns Hopkins Methods for assaying gene imprinting and methylated cpg islands
AU2002221800A1 (en) * 2000-11-02 2002-05-15 Epigenomics Ag Systems, methods and computer program products for guiding the selection of therapeutic treatment regimens
US20030143554A1 (en) * 2001-03-31 2003-07-31 Berres Mark E. Method of genotyping by determination of allele copy number
US20020197639A1 (en) * 2001-06-08 2002-12-26 Shia Michael A. Methods and products for analyzing nucleic acids based on methylation status
WO2003002752A2 (en) * 2001-06-29 2003-01-09 Rubicon Genomics Inc. Methods of using nick translate libraries for snp analysis
AU2002341891A1 (en) * 2001-09-26 2003-04-07 Epigenx Pharmaceuticals, Inc. Assays for dna methylation changes
US20030099997A1 (en) * 2001-10-24 2003-05-29 Bestor Timothy H. Method for gene identification based on differential DNA methylation
US20030232371A1 (en) * 2001-10-24 2003-12-18 Bestor Timothy H. Methods for detecting methylated promoters based on differential DNA methylation
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
WO2003064701A2 (en) * 2002-01-30 2003-08-07 Epigenomics Ag Method for the analysis of cytosine methylation patterns
EP1497447A4 (de) 2002-04-12 2006-09-20 Univ Missouri Ecist-mikroarrays für doppel-screening von dna-hypermethylierung und gen-silencing und zur verwendung in einem integrierten system zur beurteilung der genexpression, dna-methylierung und histon-acetylierung
EP1567669B1 (de) * 2002-12-02 2010-03-24 Illumina Cambridge Limited Bestimmung der methylierung von nukleinsäuresequenzen
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
US20050032102A1 (en) * 2003-07-22 2005-02-10 Affymetrix, Inc. Mapping genomic rearrangements
US20050123944A1 (en) * 2003-08-01 2005-06-09 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
DE10353419B3 (de) * 2003-11-09 2005-06-30 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA-Sequenzen mittels hemimethylierungssensitiver Restriktionsenzyme
EP2380993B1 (de) 2004-03-08 2015-12-23 Rubicon Genomics, Inc. Verfahren zur Erstellung und Erweiterung von DNA-Bibliotheken zur sensitiven Erkennung und Analyse von DNA-Methylierung
WO2005108618A2 (en) * 2004-04-30 2005-11-17 Applera Corporation Methods and kits for methylation detection
EP1632578A1 (de) 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
US20090111096A1 (en) * 2004-10-06 2009-04-30 National Institute Of Radiological Science Method of exhaustive analysis of transcriptionally-active domain (non-methylated domain) on genome
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
DK2302070T3 (da) 2005-06-23 2012-11-26 Keygene Nv Strategier til identifikation og detektion af polymorfismer med højt gennemløb
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
WO2007018602A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Isolation of cpg islands by thermal segregation and enzymatic selection-amplification method
WO2007018601A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction
WO2007037678A2 (en) 2005-09-29 2007-04-05 Keygene N.V. High throughput screening of mutagenized populations
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
EP1999271B1 (de) 2005-12-22 2013-07-03 Instytut Hodowli I Aklimatyzacji Roslin (Plant Breeding and Acclimatization Institute) Verfahren zur identifizierung induzierter variabilität in in-vitro-kulturen
CN103937899B (zh) 2005-12-22 2017-09-08 凯津公司 用于基于aflp的高通量多态性检测的方法
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
EP3239304B1 (de) 2006-04-04 2020-08-19 Keygene N.V. Nachweis von molekularmarkern mit hohem durchsatz auf der basis von aflp und mit hoher durchsatzsequenzierung
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
WO2008111453A1 (ja) * 2007-03-07 2008-09-18 The University Of Tokyo Dna断片の増幅方法
WO2008156536A1 (en) * 2007-06-20 2008-12-24 Albert Einstein College Of Medicine Of Yeshiva University Methods for determining cytosine methylation in dna and uses thereof
WO2010048337A2 (en) 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
WO2010080617A2 (en) 2008-12-19 2010-07-15 The Board Of Trustees Of The University Of Illinois Detecting and sorting methylated dna using a synthetic nanopore
US20100273164A1 (en) * 2009-03-24 2010-10-28 President And Fellows Of Harvard College Targeted and Whole-Genome Technologies to Profile DNA Cytosine Methylation
US8748091B2 (en) * 2009-12-18 2014-06-10 The Board Of Trustees Of The University Of Illinois Characterizing stretched polynucleotides in a synthetic nanopassage
WO2012162139A1 (en) 2011-05-20 2012-11-29 The Regents Of The University Of California Method to estimate age of individual based on epigenetic markers in biological sample
CA2876692C (en) 2012-06-22 2022-05-24 Quantrx Biomedical Corporation Method for obtaining fetal cells and fetal cellular components
EP3740589A4 (de) 2018-01-17 2021-11-03 The Regents of the University of California Auf phänotypischem alter und dna-methylierung basierte biomarker für lebenserwartung und morbidität
JP7320067B2 (ja) 2019-01-18 2023-08-02 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 保存された遺伝子座に基づく、哺乳動物に関するｄｎａメチル化測定
US20250210133A1 (en) 2022-03-15 2025-06-26 Genknowme S.A. Method Determining the Difference Between the Biological Age and the Chronological Age of a Subject
EP4728518A1 (de) 2023-06-15 2026-04-22 Genknowme S.A. Computerimplementiertes verfahren zur bestimmung eines wertes der allostatischen last eines menschen

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU672760B2 (en) * 1991-09-24 1996-10-17 Keygene N.V. Selective restriction fragment amplification: a general method for DNA fingerprinting
US5843649A (en) * 1995-03-01 1998-12-01 University Of Colorado Method of identifying clonal cell samples using heteroduplex generators
US5871917A (en) * 1996-05-31 1999-02-16 North Shore University Hospital Research Corp. Identification of differentially methylated and mutated nucleic acids
EP1007730A4 (de) * 1996-08-30 2004-04-28 Invitrogen Corp Verfahren zur identifizierung und isolierung von spezifischen nukleotidsequenzen in cdna und genomischer dna

Also Published As

Publication number Publication date
DE69816286T2 (de) 2004-05-27
DE69816286D1 (de) 2003-08-14
US6300071B1 (en) 2001-10-09
EP0976835A1 (de) 2000-02-02
EP0976835B1 (de) 2003-07-09

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