ATE318913T1 - System zum exprimieren einer hyperthermostabilen protease - Google Patents
System zum exprimieren einer hyperthermostabilen proteaseInfo
- Publication number
- ATE318913T1 ATE318913T1 AT98923114T AT98923114T ATE318913T1 AT E318913 T1 ATE318913 T1 AT E318913T1 AT 98923114 T AT98923114 T AT 98923114T AT 98923114 T AT98923114 T AT 98923114T AT E318913 T1 ATE318913 T1 AT E318913T1
- Authority
- AT
- Austria
- Prior art keywords
- protease
- expressing
- hyperthermostable
- hyperthermostable protease
- amino acid
- Prior art date
Links
- 108091005804 Peptidases Proteins 0.000 title abstract 5
- 239000004365 Protease Substances 0.000 title abstract 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title abstract 5
- 238000007792 addition Methods 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- 230000037431 insertion Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15196997 | 1997-06-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE318913T1 true ATE318913T1 (de) | 2006-03-15 |
Family
ID=15530186
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT98923114T ATE318913T1 (de) | 1997-06-10 | 1998-06-04 | System zum exprimieren einer hyperthermostabilen protease |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US6358726B1 (de) |
| EP (1) | EP0994191B1 (de) |
| JP (1) | JP3601835B2 (de) |
| KR (1) | KR100530598B1 (de) |
| CN (2) | CN1311075C (de) |
| AT (1) | ATE318913T1 (de) |
| AU (1) | AU7550098A (de) |
| DE (1) | DE69833652T2 (de) |
| TW (1) | TW530088B (de) |
| WO (1) | WO1998056926A1 (de) |
Families Citing this family (73)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000061711A1 (fr) * | 1999-04-07 | 2000-10-19 | Takara Shuzo Co., Ltd. | Composition de decomposition de proteines |
| DE60229361D1 (de) | 2001-03-14 | 2008-11-27 | Takara Bio Inc | Promotoren |
| CA2501645A1 (en) * | 2002-10-10 | 2004-04-22 | Verenium Corporation | Proteases, nucleic acids encoding them and methods for making and using them |
| DE10315640A1 (de) * | 2003-04-04 | 2004-10-14 | Ignatov, Konstantin | Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung |
| BRPI0410797B1 (pt) * | 2003-06-19 | 2016-10-18 | Novozymes As | polipeptídeo sintético, seqüência de ácido nucleico sintética, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para a produção de um polipeptídeo, uso de um polipeptídeo, aditivo para ração para animal, e, composição de ração |
| GB2416352B (en) * | 2004-07-21 | 2009-01-28 | Bioline Ltd | A method for performing the hot start of enzymatic reactions |
| WO2008088493A2 (en) * | 2006-12-21 | 2008-07-24 | Danisco Us, Inc., Genencor Division | Compositions and uses for an alpha-amylase polypeptide of bacillus species 195 |
| WO2010013767A1 (ja) * | 2008-07-31 | 2010-02-04 | 国立大学法人大阪大学 | 新規なプロテアーゼおよびその利用 |
| WO2010047347A1 (ja) * | 2008-10-21 | 2010-04-29 | 財団法人化学及血清療法研究所 | 封入体形成タンパク質の製造方法 |
| US9617527B2 (en) | 2010-04-14 | 2017-04-11 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| CN103608460B (zh) | 2010-12-22 | 2017-08-29 | 诺维信北美公司 | 用于产生发酵产物的工艺 |
| EP2766490B1 (de) | 2011-10-11 | 2017-04-12 | Novozymes North America, Inc. | Verflüssigungsverfahren zur herstellung von gärungsprodukten |
| EP2794873A1 (de) * | 2011-11-08 | 2014-10-29 | Novozymes A/S | Verfahren zur herstellung von archeae-protease in hefe |
| IN2014CN04905A (de) | 2011-12-02 | 2015-09-18 | Novozymes As | |
| ES2935920T3 (es) | 2012-03-30 | 2023-03-13 | Novozymes North America Inc | Procesos de elaboración de productos de fermentación |
| US9879245B2 (en) | 2012-12-07 | 2018-01-30 | Danisco Us Inc. | Polypeptides having beta-mannanase activity and methods of use |
| CA2891504A1 (en) * | 2012-12-07 | 2014-06-12 | Danisco Us Inc. | Compositions and methods of use |
| CN105339501A (zh) | 2013-06-24 | 2016-02-17 | 诺维信公司 | 用于从发酵产物过程中回收油的方法以及用于生产发酵产物的方法 |
| US11939552B2 (en) | 2013-06-24 | 2024-03-26 | Novozymes A/S | Process of recovering oil |
| JP6606068B2 (ja) * | 2013-06-25 | 2019-11-13 | ノボザイムス アクティーゼルスカブ | シグナルペプチドを含まない天然で分泌性のポリペプチドの発現 |
| DK3022300T3 (en) | 2013-07-17 | 2018-10-22 | Novozymes As | : Pullulan chimeras and polynucleotides encoding them |
| EP4717767A2 (de) | 2013-08-30 | 2026-04-01 | Novozymes A/S | Enzymzusammensetzung und verwendungen davon |
| US9951364B2 (en) | 2013-09-11 | 2018-04-24 | Novozymes A/S | Processes for producing fermentation products |
| EP3097192B1 (de) | 2014-01-22 | 2018-07-25 | Novozymes A/S | Pulluonasevarianten und polynukleotide zur codierung davon |
| ES2712402T3 (es) | 2014-06-25 | 2019-05-13 | Novozymes As | Variantes de xilanasa y polinucleótidos que las codifican |
| WO2016062875A2 (en) | 2014-10-23 | 2016-04-28 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| WO2016087327A1 (en) | 2014-12-01 | 2016-06-09 | Novozymes A/S | Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains |
| US10308963B2 (en) | 2015-02-27 | 2019-06-04 | Novozymes A/S | Processes of producing ethanol using a fermenting organism |
| US10676727B2 (en) | 2015-06-18 | 2020-06-09 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
| WO2017015329A1 (en) | 2015-07-23 | 2017-01-26 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| EP3362574B1 (de) | 2015-10-14 | 2021-07-07 | Novozymes A/S | Glycoamylasevarianten und polynukleotide zur codierung davon |
| CN108699572A (zh) | 2015-12-22 | 2018-10-23 | 诺维信公司 | 使用磷脂酶c增加发酵产物得率的方法 |
| EP3526336A1 (de) | 2016-10-17 | 2019-08-21 | Novozymes A/S | Verfahren zur schaumreduzierung während der ethanolfermentation |
| US10889836B2 (en) | 2016-11-23 | 2021-01-12 | Novozymes A/S | Yeast for ethanol production |
| EP3606936B1 (de) | 2017-04-03 | 2021-10-20 | Novozymes A/S | Verfahren zur rückgewinnung |
| BR112019021223A2 (pt) | 2017-04-11 | 2020-04-28 | Novozymes A/S | variante de glucoamilase, métodos para aumentar a atividade de hidrólise de amido em bruto de uma glucoamilase e para produzir uma variante de glucoamilase, composição, uso de uma variante de glucoamilase, processos para produzir um produto de fermentação e para produzir um produto de xarope, polinucleotídeo codificando a variante de glucoamilase, construto de ácido nucleico, vetor de expressão, e, célula hospedeira. |
| WO2018188667A1 (en) * | 2017-04-14 | 2018-10-18 | Novozymes A/S | Processes for solubilizing municipal solid waste with enzyme compositions comprising protease and enzyme compositions thereof |
| AU2018275794A1 (en) | 2017-06-02 | 2019-12-05 | Novozymes A/S | Improved yeast for ethanol production |
| DK3645555T3 (da) | 2017-06-28 | 2022-03-07 | Novozymes As | Polypeptider med trehalaseaktivitet og polynukleotider, der koder for disse |
| CA3070281A1 (en) | 2017-08-08 | 2019-02-14 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
| CN111164214A (zh) | 2017-09-15 | 2020-05-15 | 诺维信公司 | 用于改进动物饲料营养质量的酶共混物和方法 |
| US11326187B2 (en) | 2017-10-23 | 2022-05-10 | Novozymes A/S | Processes for producing a fermentation product |
| ES2954431T3 (es) | 2017-12-08 | 2023-11-22 | Novozymes As | Variantes de alfa-amilasa y polinucleótidos que codifican las mismas |
| FI3720955T3 (fi) | 2017-12-08 | 2023-09-05 | Novozymes As | Alfa-amylaasivariantteja ja niitä koodaavia polynukleotideja |
| MX2020007914A (es) | 2018-01-29 | 2020-10-28 | Novozymes As | Microorganismos con uso de nitrogeno mejorado para produccion de etanol. |
| MX2020008309A (es) | 2018-02-15 | 2020-10-14 | Novozymes As | Levadura mejorada para produccion de etanol. |
| CN112105729B (zh) | 2018-04-09 | 2024-05-14 | 诺维信公司 | 具有α-淀粉酶活性的多肽以及编码它们的多核苷酸 |
| WO2019231944A2 (en) | 2018-05-31 | 2019-12-05 | Novozymes A/S | Processes for enhancing yeast growth and productivity |
| CA3104881A1 (en) | 2018-07-11 | 2020-01-16 | Novozymes A/S | Processes for producing fermentation products |
| CN112601818A (zh) | 2018-07-25 | 2021-04-02 | 诺维信公司 | 用于生产乙醇的表达酶的酵母 |
| WO2020076697A1 (en) | 2018-10-08 | 2020-04-16 | Novozymes A/S | Enzyme-expressing yeast for ethanol production |
| WO2020160126A1 (en) | 2019-01-31 | 2020-08-06 | Novozymes A/S | Polypeptides having xylanase activity and use thereof for improving the nutritional quality of animal feed |
| CA3128139A1 (en) | 2019-03-18 | 2020-09-24 | Novozymes A/S | Polypeptides having pullulanase activity suitable for use in liquefaction |
| WO2020206058A1 (en) | 2019-04-02 | 2020-10-08 | Novozymes A/S | Process for producing a fermentation product |
| MX2021015821A (es) | 2019-07-26 | 2022-02-03 | Novozymes As | Microorganismos con transporte de nitrogeno mejorado para la produccion de etanol. |
| EP4009807A1 (de) | 2019-08-05 | 2022-06-15 | Novozymes A/S | Enzymgemische und verfahren zur herstellung eines proteinreichen futterbestandteils aus einem vollschlempennebenprodukt |
| MX2021015818A (es) | 2019-08-06 | 2022-02-03 | Novozymes As | Proteinas de fusion para mejorar la expresion de enzimas. |
| EP4031661A1 (de) | 2019-09-16 | 2022-07-27 | Novozymes A/S | Polypeptide mit beta-glucanase-aktivität und polynukleotide zur codierung davon |
| CA3158982A1 (en) | 2019-12-10 | 2021-06-17 | Monica TASSONE | Microorganism for improved pentose fermentation |
| MX2022006438A (es) | 2019-12-16 | 2022-06-22 | Novozymes As | Procesos para obtener productos de fermentacion. |
| MX2022009515A (es) | 2020-02-10 | 2022-09-02 | Novozymes As | Variantes de alfa-amilasa y polinucleotidos que las codifican. |
| WO2021163030A2 (en) | 2020-02-10 | 2021-08-19 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
| CN111876435A (zh) * | 2020-07-31 | 2020-11-03 | 山东大学 | 一种海洋嗜热胶原蛋白酶a69及其编码基因与应用 |
| AU2021370998A1 (en) | 2020-11-02 | 2023-05-25 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| BR112023025624A2 (pt) | 2021-06-07 | 2024-02-27 | Novozymes As | Célula de levedura recombinante, célula hospedeira recombinante, composição, cocultura, métodos de produção de um derivado de uma célula hospedeira recombinante e de produção de um produto de fermentação, e, uso de uma célula hospedeira recombinante |
| CN120380140A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1（ce1）多肽和编码其的多核苷酸 |
| EP4638725A1 (de) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Polypeptide der kohlenhydratesterasefamilie 3 (ce3) mit acetylxylanesteraseaktivität und polynukleotide zur codierung davon |
| WO2024137248A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization |
| EP4638767A1 (de) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Verfahren zur verringerung der sirupviskosität im backend eines verfahrens zur herstellung eines fermentationsprodukts |
| CN120283060A (zh) | 2022-12-19 | 2025-07-08 | 诺维信公司 | 用于使用纤维降解酶和工程化酵母生产发酵产物的工艺 |
| AU2024303961A1 (en) | 2023-06-13 | 2025-12-11 | Novozymes A/S | Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase |
| WO2025128568A1 (en) | 2023-12-11 | 2025-06-19 | Novozymes A/S | Composition and use thereof for increasing hemicellulosic fiber solubilization |
| WO2026008449A2 (en) | 2024-07-04 | 2026-01-08 | Novozymes A/S | A process for producing a fermentation product and a concentrated protein co-product |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1228925B (it) * | 1987-08-07 | 1991-07-10 | Eniricerche Spa | Procedimento per la preparazione dell'ormone della crescita umano |
| DE69524422T2 (de) * | 1994-06-13 | 2002-08-01 | Takara Shuzo Co., Ltd. | Gen für hyperthermostabile protease |
| CA2238296C (en) | 1995-12-12 | 2008-07-22 | Takara Shuzo Co., Ltd. | Ultrathermostable protease genes |
-
1998
- 1998-06-04 KR KR10-1999-7011547A patent/KR100530598B1/ko not_active Expired - Fee Related
- 1998-06-04 CN CNB200410101968XA patent/CN1311075C/zh not_active Expired - Fee Related
- 1998-06-04 EP EP98923114A patent/EP0994191B1/de not_active Expired - Lifetime
- 1998-06-04 AT AT98923114T patent/ATE318913T1/de not_active IP Right Cessation
- 1998-06-04 JP JP50206599A patent/JP3601835B2/ja not_active Expired - Lifetime
- 1998-06-04 WO PCT/JP1998/002465 patent/WO1998056926A1/ja not_active Ceased
- 1998-06-04 DE DE69833652T patent/DE69833652T2/de not_active Expired - Lifetime
- 1998-06-04 CN CNB988059908A patent/CN1190494C/zh not_active Expired - Fee Related
- 1998-06-04 US US09/445,472 patent/US6358726B1/en not_active Expired - Lifetime
- 1998-06-04 AU AU75500/98A patent/AU7550098A/en not_active Abandoned
- 1998-06-09 TW TW087109161A patent/TW530088B/zh not_active IP Right Cessation
-
2002
- 2002-03-06 US US10/090,624 patent/US6783970B2/en not_active Expired - Fee Related
-
2004
- 2004-07-12 US US10/888,588 patent/US7144719B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP3601835B2 (ja) | 2004-12-15 |
| TW530088B (en) | 2003-05-01 |
| AU7550098A (en) | 1998-12-30 |
| CN1260002A (zh) | 2000-07-12 |
| DE69833652D1 (de) | 2006-04-27 |
| EP0994191A1 (de) | 2000-04-19 |
| CN1190494C (zh) | 2005-02-23 |
| EP0994191A4 (de) | 2003-04-23 |
| US6783970B2 (en) | 2004-08-31 |
| US7144719B2 (en) | 2006-12-05 |
| KR20010013540A (ko) | 2001-02-26 |
| CN1657614A (zh) | 2005-08-24 |
| US6358726B1 (en) | 2002-03-19 |
| US20050084934A1 (en) | 2005-04-21 |
| CN1311075C (zh) | 2007-04-18 |
| US20020132335A1 (en) | 2002-09-19 |
| DE69833652T2 (de) | 2006-09-21 |
| WO1998056926A1 (en) | 1998-12-17 |
| KR100530598B1 (ko) | 2005-11-23 |
| EP0994191B1 (de) | 2006-03-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |