ATE318913T1 - System zum exprimieren einer hyperthermostabilen protease - Google Patents
System zum exprimieren einer hyperthermostabilen proteaseInfo
- Publication number
- ATE318913T1 ATE318913T1 AT98923114T AT98923114T ATE318913T1 AT E318913 T1 ATE318913 T1 AT E318913T1 AT 98923114 T AT98923114 T AT 98923114T AT 98923114 T AT98923114 T AT 98923114T AT E318913 T1 ATE318913 T1 AT E318913T1
- Authority
- AT
- Austria
- Prior art keywords
- protease
- expressing
- hyperthermostable
- hyperthermostable protease
- amino acid
- Prior art date
Links
- 108091005804 Peptidases Proteins 0.000 title abstract 5
- 239000004365 Protease Substances 0.000 title abstract 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title abstract 5
- 238000007792 addition Methods 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- 230000037431 insertion Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15196997 | 1997-06-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE318913T1 true ATE318913T1 (de) | 2006-03-15 |
Family
ID=15530186
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT98923114T ATE318913T1 (de) | 1997-06-10 | 1998-06-04 | System zum exprimieren einer hyperthermostabilen protease |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US6358726B1 (de) |
| EP (1) | EP0994191B1 (de) |
| JP (1) | JP3601835B2 (de) |
| KR (1) | KR100530598B1 (de) |
| CN (2) | CN1190494C (de) |
| AT (1) | ATE318913T1 (de) |
| AU (1) | AU7550098A (de) |
| DE (1) | DE69833652T2 (de) |
| TW (1) | TW530088B (de) |
| WO (1) | WO1998056926A1 (de) |
Families Citing this family (73)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000061711A1 (fr) * | 1999-04-07 | 2000-10-19 | Takara Shuzo Co., Ltd. | Composition de decomposition de proteines |
| WO2002072819A1 (fr) | 2001-03-14 | 2002-09-19 | Takara Bio, Inc. | Promoteurs |
| BR0314558A (pt) * | 2002-10-10 | 2005-08-09 | Diversa Corp | Proteases, ácidos nucléicos codificando as mesmas e processos para preparar e usar as mesmas |
| DE10315640A1 (de) * | 2003-04-04 | 2004-10-14 | Ignatov, Konstantin | Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung |
| DK1639105T4 (da) * | 2003-06-19 | 2012-12-10 | Novozymes As | Proteases |
| GB2416352B (en) * | 2004-07-21 | 2009-01-28 | Bioline Ltd | A method for performing the hot start of enzymatic reactions |
| BRPI0722093A2 (pt) * | 2006-12-21 | 2014-04-01 | Danisco Us Inc Genencor Div | Composições e usos para um polipeptídeo de alfa-amilase da espécie bacillus 195 |
| CN102112611B (zh) * | 2008-07-31 | 2013-07-24 | 国立大学法人大阪大学 | 新型蛋白酶及其应用 |
| JP5602635B2 (ja) * | 2008-10-21 | 2014-10-08 | 一般財団法人化学及血清療法研究所 | 封入体形成タンパク質の製造方法 |
| US9617527B2 (en) | 2010-04-14 | 2017-04-11 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| US9816112B2 (en) | 2010-12-22 | 2017-11-14 | Novozymes A/S | Processes for producing fermentation products |
| IN2014CN03467A (de) | 2011-10-11 | 2015-10-16 | Novozymes North America Inc | |
| CN103930544A (zh) * | 2011-11-08 | 2014-07-16 | 诺维信公司 | 酵母中生产古细菌蛋白酶的方法 |
| CN104245942A (zh) * | 2011-12-02 | 2014-12-24 | 诺维信公司 | 用于制造发酵产物的方法 |
| ES2935920T3 (es) | 2012-03-30 | 2023-03-13 | Novozymes North America Inc | Procesos de elaboración de productos de fermentación |
| BR112015012968A2 (pt) * | 2012-12-07 | 2017-09-12 | Danisco Us Inc | composições e métodos de uso |
| DK2929022T3 (en) * | 2012-12-07 | 2017-02-13 | Danisco Us Inc | COMPOSITIONS AND METHODS OF USE |
| CN105339501A (zh) * | 2013-06-24 | 2016-02-17 | 诺维信公司 | 用于从发酵产物过程中回收油的方法以及用于生产发酵产物的方法 |
| US11939552B2 (en) | 2013-06-24 | 2024-03-26 | Novozymes A/S | Process of recovering oil |
| CN105339499A (zh) * | 2013-06-25 | 2016-02-17 | 诺维信公司 | 不具有信号肽的天然分泌多肽的表达 |
| CN105339494B (zh) | 2013-07-17 | 2020-04-17 | 诺维信公司 | 普鲁兰酶嵌合体以及编码它们的多核苷酸 |
| CA2918685C (en) | 2013-08-30 | 2024-01-02 | Novozymes A/S | Enzyme composition and uses thereof |
| EP3063282B1 (de) | 2013-09-11 | 2020-03-25 | Novozymes A/S | Verfahren zur herstellung von gärungsprodukten |
| US10030237B2 (en) | 2014-01-22 | 2018-07-24 | Novozymes A/S | Pullulanase variants and polynucleotides encoding same |
| WO2015200659A1 (en) | 2014-06-25 | 2015-12-30 | Novozymes A/S | Xylanase variants and polynucleotides encoding same |
| CN106795505A (zh) | 2014-10-23 | 2017-05-31 | 诺维信公司 | 葡糖淀粉酶变体和编码它们的多核苷酸 |
| WO2016087327A1 (en) | 2014-12-01 | 2016-06-09 | Novozymes A/S | Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains |
| US10308963B2 (en) | 2015-02-27 | 2019-06-04 | Novozymes A/S | Processes of producing ethanol using a fermenting organism |
| EP3310910A1 (de) | 2015-06-18 | 2018-04-25 | Novozymes A/S | Polypeptide mit trehalaseaktivität und verwendung davon in einem verfahren zur herstellung von gärungsprodukten |
| US20180208916A1 (en) | 2015-07-23 | 2018-07-26 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| DK3362574T3 (da) | 2015-10-14 | 2021-10-11 | Novozymes As | Glucoamylasevarianter og polynukleotider, der koder for dem |
| CA3007103A1 (en) | 2015-12-22 | 2017-06-29 | Novozymes A/S | Process of extracting oil from thin stillage |
| US11015212B2 (en) | 2016-10-17 | 2021-05-25 | Novozymes A/S | Methods of reducing foam during ethanol fermentation |
| US10889836B2 (en) | 2016-11-23 | 2021-01-12 | Novozymes A/S | Yeast for ethanol production |
| US20200109388A1 (en) | 2017-04-03 | 2020-04-09 | Novozymes A/S | Recovery Process |
| CN110651048A (zh) | 2017-04-11 | 2020-01-03 | 诺维信公司 | 葡糖淀粉酶变体和编码它们的多核苷酸 |
| US20220297171A1 (en) * | 2017-04-14 | 2022-09-22 | Novozymes A/S | Processes For Solubilizing Municipal Solid Waste With Enzyme Compositions Comprising Protease And Enzyme Compositions Thereof |
| WO2018222990A1 (en) | 2017-06-02 | 2018-12-06 | Novozymes A/S | Improved yeast for ethanol production |
| US11584783B2 (en) | 2017-06-28 | 2023-02-21 | Novozymes A/S | Processes for producing a fermentation product |
| EP3665275A1 (de) | 2017-08-08 | 2020-06-17 | Novozymes A/S | Polypeptide mit trehalaseaktivität und verwendung davon in einem verfahren zur herstellung von gärungsprodukten |
| EP3682014A1 (de) | 2017-09-15 | 2020-07-22 | Novozymes A/S | Enzymgemische und verfahren zur verbesserung der nährstoffqualität von tierfutter |
| EP3701037A1 (de) | 2017-10-23 | 2020-09-02 | Novozymes A/S | Verfahren zur verrringerung von milchsäure in einem biokraftstofffermentationssystem |
| ES2954431T3 (es) | 2017-12-08 | 2023-11-22 | Novozymes As | Variantes de alfa-amilasa y polinucleótidos que codifican las mismas |
| MX2020005458A (es) | 2017-12-08 | 2020-08-27 | Novozymes As | Variantes de alfa-amilasa y polinucleotidos que codifican las mismas. |
| MX2020007914A (es) | 2018-01-29 | 2020-10-28 | Novozymes As | Microorganismos con uso de nitrogeno mejorado para produccion de etanol. |
| BR112020016677A2 (pt) | 2018-02-15 | 2020-12-15 | Novozymes A/S | Levedura melhorada para a produção de etanol |
| CN112105729B (zh) | 2018-04-09 | 2024-05-14 | 诺维信公司 | 具有α-淀粉酶活性的多肽以及编码它们的多核苷酸 |
| CN112166197A (zh) | 2018-05-31 | 2021-01-01 | 诺维信公司 | 用于增强酵母生长和生产力的方法 |
| CN112368393A (zh) | 2018-07-11 | 2021-02-12 | 诺维信公司 | 用于生产发酵产物的方法 |
| MX2021000893A (es) | 2018-07-25 | 2021-03-31 | Novozymes As | Levadura que expresa enzimas para la producción de etanol. |
| MX2021003955A (es) | 2018-10-08 | 2021-05-27 | Novozymes As | Levadura que expresa enzimas para la produccion de etanol. |
| BR112021014873A2 (pt) | 2019-01-31 | 2021-10-05 | Novozymes A/S | Polipeptídeo, combinação de enzimas, polinucleotídeo, construto de ácido nucleico ou vetor de expressão recombinante, célula hospedeira recombinante, método de produção de um polipeptídeo, e, processo de produção de um produto de fermentação |
| CN113490740A (zh) | 2019-03-18 | 2021-10-08 | 诺维信公司 | 适合用于液化中的具有普鲁兰酶活性的多肽 |
| BR112021017085A2 (pt) | 2019-04-02 | 2022-02-08 | Novozymes As | Processo de produção de um produto de fermentação |
| AU2020321930A1 (en) | 2019-07-26 | 2022-01-06 | Novozymes A/S | Microorganisms with improved nitrogen transport for ethanol production |
| BR112022002203A2 (pt) | 2019-08-05 | 2022-09-06 | Novozymes As | Misturas de enzimas e processos para produção de um ingrediente alimentar com alto teor de proteína a partir de um subproduto de vinhaça completa |
| WO2021025872A1 (en) | 2019-08-06 | 2021-02-11 | Novozymes A/S | Fusion proteins for improved enzyme expression |
| PY2055454A (es) | 2019-09-16 | 2022-03-31 | Novozymes As | Polipéptidos que poseen actividad de beta-glucanasa y polinucleótidos que los codifican |
| CA3158982A1 (en) | 2019-12-10 | 2021-06-17 | Monica TASSONE | Microorganism for improved pentose fermentation |
| BR112022011634A2 (pt) | 2019-12-16 | 2022-08-30 | Novozymes As | Processos para produção de um produto de fermentação, para diminuição da quantidade de amido residual presente em um liquefato e para aumento da quantidade de oligossacarídeos de cadeia curta presente em um liquefato |
| CA3166233A1 (en) | 2020-02-10 | 2021-08-19 | Sarah Schultheis ELLIOTT | Alpha-amylase variants and polynucleotides encoding same |
| PY2110525A (es) | 2020-02-10 | 2022-05-11 | Novozymes As | Polipéptidos con actividad de alfa amilasa y polinucleótidos que los codifican |
| CN111876435A (zh) * | 2020-07-31 | 2020-11-03 | 山东大学 | 一种海洋嗜热胶原蛋白酶a69及其编码基因与应用 |
| CA3199315A1 (en) | 2020-11-02 | 2022-05-05 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| BR112023025624A2 (pt) | 2021-06-07 | 2024-02-27 | Novozymes As | Célula de levedura recombinante, célula hospedeira recombinante, composição, cocultura, métodos de produção de um derivado de uma célula hospedeira recombinante e de produção de um produto de fermentação, e, uso de uma célula hospedeira recombinante |
| EP4638725A1 (de) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Polypeptide der kohlenhydratesterasefamilie 3 (ce3) mit acetylxylanesteraseaktivität und polynukleotide zur codierung davon |
| JP2025541374A (ja) | 2022-12-19 | 2025-12-18 | ノボザイムス アクティーゼルスカブ | アラビノフラノシダーゼ及びキシラナーゼを含む組成物、並びにヘミセルロース系繊維の可溶化を増加させるためのその使用 |
| EP4638768A2 (de) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Verfahren zur herstellung von fermentationsprodukten unter verwendung faserabbauender enzyme mit manipulierter hefe |
| EP4638724A1 (de) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Polypeptide der kohlenhydratesterasefamilie 1 (ce1) mit ferulasäureesterase- und/oder acetylxylanesteraseaktivität und dafür codierende polynukleotide |
| CN120380158A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 用于降低发酵产物生产方法后端的浆体粘度的方法 |
| AU2024303961A1 (en) | 2023-06-13 | 2025-12-11 | Novozymes A/S | Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase |
| WO2025128568A1 (en) | 2023-12-11 | 2025-06-19 | Novozymes A/S | Composition and use thereof for increasing hemicellulosic fiber solubilization |
| WO2026008449A2 (en) | 2024-07-04 | 2026-01-08 | Novozymes A/S | A process for producing a fermentation product and a concentrated protein co-product |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1228925B (it) * | 1987-08-07 | 1991-07-10 | Eniricerche Spa | Procedimento per la preparazione dell'ormone della crescita umano |
| DK0776971T3 (da) * | 1994-06-13 | 2002-04-02 | Takara Shuzo Co | Hyperthermostabilt proteasegen |
| US6261822B1 (en) | 1995-12-12 | 2001-07-17 | Takara Shuzo Co., Ltd. | Ultrathermostable protease genes |
-
1998
- 1998-06-04 CN CNB988059908A patent/CN1190494C/zh not_active Expired - Fee Related
- 1998-06-04 CN CNB200410101968XA patent/CN1311075C/zh not_active Expired - Fee Related
- 1998-06-04 AU AU75500/98A patent/AU7550098A/en not_active Abandoned
- 1998-06-04 AT AT98923114T patent/ATE318913T1/de not_active IP Right Cessation
- 1998-06-04 WO PCT/JP1998/002465 patent/WO1998056926A1/ja not_active Ceased
- 1998-06-04 EP EP98923114A patent/EP0994191B1/de not_active Expired - Lifetime
- 1998-06-04 KR KR10-1999-7011547A patent/KR100530598B1/ko not_active Expired - Fee Related
- 1998-06-04 US US09/445,472 patent/US6358726B1/en not_active Expired - Lifetime
- 1998-06-04 JP JP50206599A patent/JP3601835B2/ja not_active Expired - Lifetime
- 1998-06-04 DE DE69833652T patent/DE69833652T2/de not_active Expired - Lifetime
- 1998-06-09 TW TW087109161A patent/TW530088B/zh not_active IP Right Cessation
-
2002
- 2002-03-06 US US10/090,624 patent/US6783970B2/en not_active Expired - Fee Related
-
2004
- 2004-07-12 US US10/888,588 patent/US7144719B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP3601835B2 (ja) | 2004-12-15 |
| AU7550098A (en) | 1998-12-30 |
| DE69833652D1 (de) | 2006-04-27 |
| US20050084934A1 (en) | 2005-04-21 |
| EP0994191A1 (de) | 2000-04-19 |
| TW530088B (en) | 2003-05-01 |
| KR100530598B1 (ko) | 2005-11-23 |
| US6358726B1 (en) | 2002-03-19 |
| US7144719B2 (en) | 2006-12-05 |
| WO1998056926A1 (en) | 1998-12-17 |
| CN1260002A (zh) | 2000-07-12 |
| US6783970B2 (en) | 2004-08-31 |
| EP0994191A4 (de) | 2003-04-23 |
| US20020132335A1 (en) | 2002-09-19 |
| EP0994191B1 (de) | 2006-03-01 |
| CN1190494C (zh) | 2005-02-23 |
| DE69833652T2 (de) | 2006-09-21 |
| CN1657614A (zh) | 2005-08-24 |
| CN1311075C (zh) | 2007-04-18 |
| KR20010013540A (ko) | 2001-02-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |