ATE318913T1 - System zum exprimieren einer hyperthermostabilen protease - Google Patents

System zum exprimieren einer hyperthermostabilen protease

Info

Publication number
ATE318913T1
ATE318913T1 AT98923114T AT98923114T ATE318913T1 AT E318913 T1 ATE318913 T1 AT E318913T1 AT 98923114 T AT98923114 T AT 98923114T AT 98923114 T AT98923114 T AT 98923114T AT E318913 T1 ATE318913 T1 AT E318913T1
Authority
AT
Austria
Prior art keywords
protease
expressing
hyperthermostable
hyperthermostable protease
amino acid
Prior art date
Application number
AT98923114T
Other languages
English (en)
Inventor
Hikaru Takakura
Mio Morishita
Tomoko Shimojo
Kiyozo Asada
Ikunoshin Kato
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Application granted granted Critical
Publication of ATE318913T1 publication Critical patent/ATE318913T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT98923114T 1997-06-10 1998-06-04 System zum exprimieren einer hyperthermostabilen protease ATE318913T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15196997 1997-06-10

Publications (1)

Publication Number Publication Date
ATE318913T1 true ATE318913T1 (de) 2006-03-15

Family

ID=15530186

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98923114T ATE318913T1 (de) 1997-06-10 1998-06-04 System zum exprimieren einer hyperthermostabilen protease

Country Status (10)

Country Link
US (3) US6358726B1 (de)
EP (1) EP0994191B1 (de)
JP (1) JP3601835B2 (de)
KR (1) KR100530598B1 (de)
CN (2) CN1311075C (de)
AT (1) ATE318913T1 (de)
AU (1) AU7550098A (de)
DE (1) DE69833652T2 (de)
TW (1) TW530088B (de)
WO (1) WO1998056926A1 (de)

Families Citing this family (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3455000A (en) * 1999-04-07 2000-11-14 Takara Shuzo Co., Ltd. Composition for decomposing protein
EP1371724B1 (de) 2001-03-14 2008-10-15 Takara Bio Inc. Promotoren
MX282672B (es) * 2002-10-10 2011-01-10 Diversa Corp Proteasas, acidos nucleicos que los codifican, y metodos de hacer y usar los mismos.
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
ATE408682T1 (de) * 2003-06-19 2008-10-15 Novozymes As Proteasen
GB2416352B (en) * 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
BRPI0722093A2 (pt) * 2006-12-21 2014-04-01 Danisco Us Inc Genencor Div Composições e usos para um polipeptídeo de alfa-amilase da espécie bacillus 195
JP5339543B2 (ja) * 2008-07-31 2013-11-13 国立大学法人大阪大学 新規なプロテアーゼおよびその利用
WO2010047347A1 (ja) * 2008-10-21 2010-04-29 財団法人化学及血清療法研究所 封入体形成タンパク質の製造方法
US9617527B2 (en) 2010-04-14 2017-04-11 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
US9816112B2 (en) 2010-12-22 2017-11-14 Novozymes A/S Processes for producing fermentation products
CA2861678C (en) 2011-10-11 2021-02-16 Novozymes North America, Inc. Processes for producing fermentation products
WO2013068309A1 (en) * 2011-11-08 2013-05-16 Novozymes A/S Methods for production of archeae protease in yeast
IN2014CN04905A (de) 2011-12-02 2015-09-18 Novozymes As
ES2935920T3 (es) 2012-03-30 2023-03-13 Novozymes North America Inc Procesos de elaboración de productos de fermentación
BR112015012986A2 (pt) * 2012-12-07 2017-09-12 Danisco Us Inc composições e métodos de uso
BR112015012968A2 (pt) * 2012-12-07 2017-09-12 Danisco Us Inc composições e métodos de uso
US11939552B2 (en) 2013-06-24 2024-03-26 Novozymes A/S Process of recovering oil
US10035973B2 (en) * 2013-06-24 2018-07-31 Novozymes A/S Process of extracting oil from thin stillage
JP6606068B2 (ja) * 2013-06-25 2019-11-13 ノボザイムス アクティーゼルスカブ シグナルペプチドを含まない天然で分泌性のポリペプチドの発現
EP3022300B1 (de) 2013-07-17 2018-07-11 Novozymes A/S Pullanase chimaeren und dafür kodierende nukleinsäuren
EP3039131B1 (de) 2013-08-30 2026-01-21 Novozymes A/S Enzymzusammensetzung und verwendungen davon
CN105934518A (zh) 2013-09-11 2016-09-07 诺维信公司 用于生产发酵产品的方法
EP3097192B1 (de) 2014-01-22 2018-07-25 Novozymes A/S Pulluonasevarianten und polynukleotide zur codierung davon
WO2015200659A1 (en) 2014-06-25 2015-12-30 Novozymes A/S Xylanase variants and polynucleotides encoding same
WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
WO2016087327A1 (en) 2014-12-01 2016-06-09 Novozymes A/S Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains
AU2016225049B2 (en) 2015-02-27 2022-03-31 Microbiogen Pty. Ltd. Processes of producing ethanol using a fermenting organism
CA2981342A1 (en) 2015-06-18 2016-12-22 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
WO2017015329A1 (en) 2015-07-23 2017-01-26 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
US10494685B2 (en) 2015-10-14 2019-12-03 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US10597645B2 (en) 2015-12-22 2020-03-24 Novozymes A/S Process of extracting oil from thin stillage
CN110088287A (zh) 2016-10-17 2019-08-02 诺维信公司 在乙醇发酵过程中减少泡沫的方法
US10889836B2 (en) 2016-11-23 2021-01-12 Novozymes A/S Yeast for ethanol production
US20200109388A1 (en) 2017-04-03 2020-04-09 Novozymes A/S Recovery Process
BR112019021223A2 (pt) 2017-04-11 2020-04-28 Novozymes A/S variante de glucoamilase, métodos para aumentar a atividade de hidrólise de amido em bruto de uma glucoamilase e para produzir uma variante de glucoamilase, composição, uso de uma variante de glucoamilase, processos para produzir um produto de fermentação e para produzir um produto de xarope, polinucleotídeo codificando a variante de glucoamilase, construto de ácido nucleico, vetor de expressão, e, célula hospedeira.
WO2018188667A1 (en) * 2017-04-14 2018-10-18 Novozymes A/S Processes for solubilizing municipal solid waste with enzyme compositions comprising protease and enzyme compositions thereof
CN110730824A (zh) 2017-06-02 2020-01-24 诺维信公司 用于乙醇生产的改善的酵母
US11584783B2 (en) 2017-06-28 2023-02-21 Novozymes A/S Processes for producing a fermentation product
US11220679B2 (en) 2017-08-08 2022-01-11 Novozymes A/S Polypeptides having trehalase activity
CA3070730A1 (en) 2017-09-15 2019-03-21 Novozymes A/S Enzyme blends and processes for improving the nutritional quality of animal feed
CA3075907A1 (en) 2017-10-23 2019-05-02 Novozymes A/S Processes for reducing lactic acid in a biofuel fermentation system
DK3720955T5 (da) 2017-12-08 2024-08-26 Novozymes As Alfa-amylasevarianter og polynukleotider, der koder for dem
BR112020011173A2 (pt) 2017-12-08 2020-11-17 Novozymes A/S variante de alfa-amilase, polinucleotídeo, composição, construto de ácido nucleico, vetor de expressão, célula hospedeira, método de produção de uma variante de alfa-amilase, uso da variante, e, processo para produção de um xarope a partir de material contendo amido.
EP3746545A1 (de) 2018-01-29 2020-12-09 Novozymes A/S Mikroorganismen mit verbesserter stickstoffausnutzung für die ethanolproduktion
AU2019222480B9 (en) 2018-02-15 2024-11-28 Microbiogen Pty. Ltd. Improved yeast for ethanol production
WO2019197318A1 (en) 2018-04-09 2019-10-17 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
WO2019231944A2 (en) 2018-05-31 2019-12-05 Novozymes A/S Processes for enhancing yeast growth and productivity
US20210269833A1 (en) 2018-07-11 2021-09-02 Novozymes A/S Processes for producing fermentation products
MX2021000893A (es) 2018-07-25 2021-03-31 Novozymes As Levadura que expresa enzimas para la producción de etanol.
CN113286889A (zh) 2018-10-08 2021-08-20 诺维信公司 用于生产乙醇的表达酶的酵母
BR112021014873A2 (pt) 2019-01-31 2021-10-05 Novozymes A/S Polipeptídeo, combinação de enzimas, polinucleotídeo, construto de ácido nucleico ou vetor de expressão recombinante, célula hospedeira recombinante, método de produção de um polipeptídeo, e, processo de produção de um produto de fermentação
EP3942033A1 (de) 2019-03-18 2022-01-26 Novozymes A/S Polypeptide mit pullulanaseaktivität zur verwendung in der verflüssigung
BR112021017085A2 (pt) 2019-04-02 2022-02-08 Novozymes As Processo de produção de um produto de fermentação
WO2021021458A1 (en) 2019-07-26 2021-02-04 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
WO2021026201A1 (en) 2019-08-05 2021-02-11 Novozymes A/S Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct
MX2021015818A (es) 2019-08-06 2022-02-03 Novozymes As Proteinas de fusion para mejorar la expresion de enzimas.
EP4031661A1 (de) 2019-09-16 2022-07-27 Novozymes A/S Polypeptide mit beta-glucanase-aktivität und polynukleotide zur codierung davon
CA3158982A1 (en) 2019-12-10 2021-06-17 Monica TASSONE Microorganism for improved pentose fermentation
PY2084892A (es) 2019-12-16 2022-07-26 Novozymes As Proceso para producir productos de fermentación
US20230142991A1 (en) 2020-02-10 2023-05-11 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
EP4103709A2 (de) 2020-02-10 2022-12-21 Novozymes A/S Polypeptide, die alpha-amylase-aktivität aufweisen, und für dieselben codierende polynukleotide
CN111876435A (zh) * 2020-07-31 2020-11-03 山东大学 一种海洋嗜热胶原蛋白酶a69及其编码基因与应用
CN116438309A (zh) 2020-11-02 2023-07-14 诺维信公司 葡糖淀粉酶变体和编码它们的多核苷酸
AU2022288057A1 (en) 2021-06-07 2023-12-21 Novozymes A/S Engineered microorganism for improved ethanol fermentation
EP4638768A2 (de) 2022-12-19 2025-10-29 Novozymes A/S Verfahren zur herstellung von fermentationsprodukten unter verwendung faserabbauender enzyme mit manipulierter hefe
EP4638725A1 (de) 2022-12-19 2025-10-29 Novozymes A/S Polypeptide der kohlenhydratesterasefamilie 3 (ce3) mit acetylxylanesteraseaktivität und polynukleotide zur codierung davon
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
EP4638724A1 (de) 2022-12-19 2025-10-29 Novozymes A/S Polypeptide der kohlenhydratesterasefamilie 1 (ce1) mit ferulasäureesterase- und/oder acetylxylanesteraseaktivität und dafür codierende polynukleotide
JP2025541374A (ja) 2022-12-19 2025-12-18 ノボザイムス アクティーゼルスカブ アラビノフラノシダーゼ及びキシラナーゼを含む組成物、並びにヘミセルロース系繊維の可溶化を増加させるためのその使用
WO2024258820A2 (en) 2023-06-13 2024-12-19 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization
WO2026008449A2 (en) 2024-07-04 2026-01-08 Novozymes A/S A process for producing a fermentation product and a concentrated protein co-product

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1228925B (it) * 1987-08-07 1991-07-10 Eniricerche Spa Procedimento per la preparazione dell'ormone della crescita umano
ATE210181T1 (de) * 1994-06-13 2001-12-15 Takara Shuzo Co Gen für hyperthermostabile protease
CN1128879C (zh) * 1995-12-12 2003-11-26 宝酒造株式会社 超耐热蛋白酶基因

Also Published As

Publication number Publication date
CN1311075C (zh) 2007-04-18
CN1190494C (zh) 2005-02-23
JP3601835B2 (ja) 2004-12-15
AU7550098A (en) 1998-12-30
TW530088B (en) 2003-05-01
US20020132335A1 (en) 2002-09-19
CN1657614A (zh) 2005-08-24
US6358726B1 (en) 2002-03-19
US20050084934A1 (en) 2005-04-21
EP0994191A1 (de) 2000-04-19
US6783970B2 (en) 2004-08-31
KR20010013540A (ko) 2001-02-26
CN1260002A (zh) 2000-07-12
WO1998056926A1 (en) 1998-12-17
EP0994191A4 (de) 2003-04-23
KR100530598B1 (ko) 2005-11-23
US7144719B2 (en) 2006-12-05
EP0994191B1 (de) 2006-03-01
DE69833652T2 (de) 2006-09-21
DE69833652D1 (de) 2006-04-27

Similar Documents

Publication Publication Date Title
DE69833652D1 (de) System zum exprimieren einer hyperthermostabilen protease
ATE169678T1 (de) Stabilisierte enzyme
ATE399858T1 (de) Blütenorgan-spezifische promotersequenzen
EP0205475A4 (de) Rekombinantverfahren zur herstellung von serinproteaseinhibitoren, sowie dns-sequenzen dazu.
EP1037999A4 (de) Porphorymonas gingivalis polypeptide und polynukleotide
NO971621L (no) Keratinocyttvekstfaktoranaloger
DE60237791D1 (de) Zytokinproteinfamilie
DE69615055D1 (de) Gene kodierend für ultrathermostabile proteasen
ATE286972T1 (de) Für phosphoenolpyruvatcarboxykinase kodierende dna, sie enthaltender rekombinanter vektor und transgene pflanze
DE59109072D1 (de) Klonierung der N-Methylhydantoinase
ATE121456T1 (de) Klonierung und überexpression von glucose-6- phosphat-dehydrogenase aus leuconostoc dextranicus.
DK0828004T3 (da) Proteindisulfidisomerase-gen afledt af stamme af methylotrof gær
ATE301199T1 (de) Beta-primeverosidase gen
ATE180015T1 (de) N-carbamoylsarcosin-amidohydrolase, kloniert
EP0945512A3 (de) Serinproteasen
LV12722A (lv) Prv-1 gene and the use thereof
DK210288A (da) Overproduktion af exocelllulaere proteiner
EP1295943A4 (de) Polypeptide mit aktivität der stimulierung von neutrophilen
GB0008504D0 (en) Novel polypeptide

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties