ATE321145T1 - Herstellung von ascorbinsäure in hefen - Google Patents

Herstellung von ascorbinsäure in hefen

Info

Publication number: ATE321145T1
Authority: AT; Austria
Prior art keywords: ascorbic acid; yeast; lactone; medium; acid precursor
Prior art date: 2000-08-02

Application number

AT01953269T

Other languages

English (en)

Inventor

Danilo Porro

Michael Sauer

Original Assignee

Biopolo S C A R L

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-08-02

Filing date

2001-08-02

Publication date

2006-04-15

2001-08-02 Application filed by Biopolo S C A R L filed Critical Biopolo S C A R L

2006-04-15 Application granted granted Critical

2006-04-15 Publication of ATE321145T1 publication Critical patent/ATE321145T1/de

Links

CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 title abstract 16
229960005070 ascorbic acid Drugs 0.000 title abstract 8
240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 7
235000010323 ascorbic acid Nutrition 0.000 title abstract 7
239000011668 ascorbic acid Substances 0.000 title abstract 7
239000002243 precursor Substances 0.000 abstract 4
108091026890 Coding region Proteins 0.000 abstract 2
108010071317 D-arabinonolactone oxidase Proteins 0.000 abstract 2
108010071625 D-arabinose dehydrogenase Proteins 0.000 abstract 2
125000002353 D-glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 abstract 1
101710088194 Dehydrogenase Proteins 0.000 abstract 1
108090000790 Enzymes Proteins 0.000 abstract 1
102000004190 Enzymes Human genes 0.000 abstract 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
235000000069 L-ascorbic acid Nutrition 0.000 abstract 1
239000002211 L-ascorbic acid Substances 0.000 abstract 1
SXZYCXMUPBBULW-NEEWWZBLSA-N L-galactono-1,4-lactone Chemical compound OC[C@H](O)[C@H]1OC(=O)[C@@H](O)[C@@H]1O SXZYCXMUPBBULW-NEEWWZBLSA-N 0.000 abstract 1
WQZGKKKJIJFFOK-DHVFOXMCSA-N L-galactose Chemical compound OC[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O WQZGKKKJIJFFOK-DHVFOXMCSA-N 0.000 abstract 1
RGHNJXZEOKUKBD-QTBDOELSSA-N L-gulonic acid Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O RGHNJXZEOKUKBD-QTBDOELSSA-N 0.000 abstract 1
SXZYCXMUPBBULW-SKNVOMKLSA-N L-gulono-1,4-lactone Chemical compound OC[C@H](O)[C@H]1OC(=O)[C@@H](O)[C@H]1O SXZYCXMUPBBULW-SKNVOMKLSA-N 0.000 abstract 1
241000235070 Saccharomyces Species 0.000 abstract 1
241000235017 Zygosaccharomyces Species 0.000 abstract 1
PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 abstract 1
SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Medicinal Preparation (AREA)

AT01953269T 2000-08-02 2001-08-02 Herstellung von ascorbinsäure in hefen ATE321145T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US09/630,983 US6630330B1 (en)	2000-08-02	2000-08-02	Ascorbic acid production from yeast

Publications (1)

Publication Number	Publication Date
ATE321145T1 true ATE321145T1 (de)	2006-04-15

Family

ID=24529341

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
AT04023458T ATE357533T1 (de)	2000-08-02	2001-08-02	Herstellung von ascorbinsäure in hefen
AT01953269T ATE321145T1 (de)	2000-08-02	2001-08-02	Herstellung von ascorbinsäure in hefen

Family Applications Before (1)

Application Number	Title	Priority Date	Filing Date
AT04023458T ATE357533T1 (de)	2000-08-02	2001-08-02	Herstellung von ascorbinsäure in hefen

Country Status (12)

Country	Link
US (3)	US6630330B1 (de)
EP (3)	EP1820863A1 (de)
JP (1)	JP2004504850A (de)
CN (1)	CN100447249C (de)
AT (2)	ATE357533T1 (de)
AU (1)	AU2001275757A1 (de)
BR (1)	BR0113145A (de)
CA (1)	CA2416142A1 (de)
DE (2)	DE60118200T2 (de)
ES (2)	ES2260249T3 (de)
MX (1)	MXPA03000945A (de)
WO (1)	WO2002010425A2 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP1465988A4 (de)	2001-03-19	2005-08-03	Cargill Inc	Myoinositol-oxygenasen
ES2224773B1 (es) *	2001-12-27	2005-12-16	Universidad De Malaga	Construccion de adn y metodo para incrementar la produccion de vitamina c en una planta.
US20060166339A1 (en) *	2002-09-27	2006-07-27	Tatsuo Hoshino	Production of l-aldonolactone
DE10252245A1 (de) *	2002-11-07	2004-05-27	Prof. Dr. Danilo Porro Università degli Studi di Milano-Bicocca Dipartimento die Biotechnologie e Bioscienze	Verfahren zur Expression und Sekretion von Proteinen mittels der nicht-konventionellen Hefe Zygosaccharomyces bailii
US20060234360A1 (en) *	2005-04-13	2006-10-19	Paola Branduardi	Ascorbic acid production from D-glucose in yeast
JP2007135445A (ja) *	2005-11-16	2007-06-07	Tokyo Univ Of Agriculture & Technology	エリスロアスコルビン酸の製造方法
DE102010009104A1 (de) *	2010-02-24	2011-08-25	Epcos Ag, 81669	Detektorschaltung
WO2012103263A2 (en) *	2011-01-25	2012-08-02	Finley Kenneth R	Compositions and methods for malate and fumarate production
CA2825525A1 (en)	2011-01-25	2012-08-02	Jeanette M. HURYTA	Compositions and methods for succinate production
KR101525298B1 (ko) *	2012-01-18	2015-06-03	고려대학교 산학협력단	3,6-안하이드로-l-갈락토오스의 제조방법 및 이의 용도
US9850507B2 (en)	2012-07-25	2017-12-26	Cargill, Incorporated	Yeast cells having reductive TCA pathway from pyruvate to succinate and overexpressing an exogenous NAD(P)+ transhydrogenase enzyme
WO2018053004A2 (en)	2016-09-13	2018-03-22	Allergan, Inc.	Non-protein clostridial toxin compositions
US11384135B2 (en)	2017-09-22	2022-07-12	Modern Meadow, Inc.	Recombinant yeast strains
CN120210127A (zh) *	2019-05-31	2025-06-27	塔夫茨大学信托人	使用基因修饰细胞的培养肉产品
CN110564660B (zh) *	2019-09-18	2023-03-21	苏州华赛生物工程技术有限公司	生产乳清酸的重组微生物及方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU3102784A (en) *	1983-06-27	1985-01-25	Hostettler, F.	Low temperature, storage stable mixtures of prepolymers and flow modifiers
US4916068A (en)	1983-10-20	1990-04-10	Kraft, Inc.	Bioconversion production of ascorbic acid with L-galactono-1,4-oxidase
US4595659A (en)	1983-10-20	1986-06-17	Kraft, Inc.	Fermentation production of ascorbic acid from L-galactonic substrate
WO1998050558A2 (en)	1997-05-07	1998-11-12	Vlaams Interuniversitair Instituut Voor Biotechnologie	Production of ascorbic acid in plants
EP1042486A1 (de)	1997-12-23	2000-10-11	Ascorbex Limited	Pflanzliche galaktose-dehydrogenase
US20020012979A1 (en) *	1998-06-08	2002-01-31	Alan Berry	Vitamin c production in microorganisms and plants
US6358715B1 (en)	1998-12-04	2002-03-19	Genencor International, Inc.	Production of ascorbic acid

2000
- 2000-08-02 US US09/630,983 patent/US6630330B1/en not_active Expired - Fee Related
2001
- 2001-08-02 ES ES01953269T patent/ES2260249T3/es not_active Expired - Lifetime
- 2001-08-02 EP EP07001271A patent/EP1820863A1/de not_active Withdrawn
- 2001-08-02 EP EP04023458A patent/EP1498489B1/de not_active Expired - Lifetime
- 2001-08-02 CN CNB018147909A patent/CN100447249C/zh not_active Expired - Fee Related
- 2001-08-02 AU AU2001275757A patent/AU2001275757A1/en not_active Abandoned
- 2001-08-02 AT AT04023458T patent/ATE357533T1/de not_active IP Right Cessation
- 2001-08-02 JP JP2002516341A patent/JP2004504850A/ja not_active Withdrawn
- 2001-08-02 WO PCT/GB2001/003485 patent/WO2002010425A2/en not_active Ceased
- 2001-08-02 BR BR0113145-1A patent/BR0113145A/pt not_active IP Right Cessation
- 2001-08-02 ES ES04023458T patent/ES2285327T3/es not_active Expired - Lifetime
- 2001-08-02 EP EP01953269A patent/EP1307578B1/de not_active Expired - Lifetime
- 2001-08-02 CA CA002416142A patent/CA2416142A1/en not_active Abandoned
- 2001-08-02 DE DE60118200T patent/DE60118200T2/de not_active Expired - Fee Related
- 2001-08-02 DE DE60127470T patent/DE60127470T2/de not_active Expired - Fee Related
- 2001-08-02 AT AT01953269T patent/ATE321145T1/de not_active IP Right Cessation
- 2001-08-02 MX MXPA03000945A patent/MXPA03000945A/es active IP Right Grant
2003
- 2003-06-25 US US10/606,302 patent/US7579171B2/en not_active Expired - Fee Related
- 2003-06-25 US US10/606,300 patent/US20050019879A1/en not_active Abandoned

Also Published As

Publication number	Publication date
EP1307578B1 (de)	2006-03-22
WO2002010425A3 (en)	2002-10-24
CN100447249C (zh)	2008-12-31
US7579171B2 (en)	2009-08-25
AU2001275757A1 (en)	2002-02-13
US20050019879A1 (en)	2005-01-27
ATE357533T1 (de)	2007-04-15
EP1498489B1 (de)	2007-03-21
ES2285327T3 (es)	2007-11-16
WO2002010425A2 (en)	2002-02-07
EP1820863A1 (de)	2007-08-22
EP1498489A2 (de)	2005-01-19
US6630330B1 (en)	2003-10-07
JP2004504850A (ja)	2004-02-19
EP1307578A2 (de)	2003-05-07
DE60127470D1 (de)	2007-05-03
DE60118200D1 (de)	2006-05-11
DE60127470T2 (de)	2007-11-29
CA2416142A1 (en)	2002-02-07
ES2260249T3 (es)	2006-11-01
BR0113145A (pt)	2003-06-10
CN1484708A (zh)	2004-03-24
MXPA03000945A (es)	2004-08-02
US20050260722A1 (en)	2005-11-24
EP1498489A3 (de)	2005-04-13
DE60118200T2 (de)	2007-04-12

Legal Events

Date

Code

Title

Description

2007-02-15

UEP

Publication of translation of european patent specification

Ref document number: 1307578

Country of ref document: EP

2010-05-15

REN

Ceased due to non-payment of the annual fee

Publication	Publication Date	Title
ATE321145T1 (de)	2006-04-15	Herstellung von ascorbinsäure in hefen
ES2278181T3 (es)	2007-08-01	Produccion microbiana de vitamina c.
DE69333546D1 (de)	2004-07-15	Verbesserte enzyme zur herstellung von 2-keto-l-gulonsäure
MX170983B (es)	1993-09-23	Metodo para la preparacion de un extracto de levadura, este extracto de levadura se usa como un saborizante alimenticio
ATE404664T1 (de)	2008-08-15	Aldehyddehyrdrogenase-gen
ID22130A (id)	1999-09-09	Bakteri strain-strain baru dan penggunaannya didalam fermentasi pada proses pembuatan asam 2-keto-l-gulonat
DK1141368T3 (da)	2006-09-18	Fremgangsmåde til produktion af ascorbinsyremellemprodukter
WO1989006688A3 (en)	1989-09-21	Novel enzyme
DE69637038D1 (de)	2007-06-06	D-Sorbitol-Dehydrogenase
ATE78297T1 (de)	1992-08-15	Herstellung eines vitamin-c-vorlaeufers mittels genetisch modifizierter organismen.
US6846660B2 (en)	2005-01-25	Method of making an aldehyde dehydrogenase with gluconobacter
CA2353558A1 (en)	2000-06-15	Production of ascorbic acid using yeast
WO2001072974A3 (en)	2002-01-31	L-galactose dehydrogenase from arabidopsis thaliana, and uses thereof
WO2000061727A3 (de)	2001-02-01	Herstellung von pankreatischer procarboxypeptidase b, isoformen und muteinen davon und ihre verwendung
EP0955358A3 (de)	2000-03-15	Genetisch veränderte, L-Sorbose Reduktase defiziente Mutanten
EP1426367A4 (de)	2005-04-13	Lactosederivat sowie pflanzenwachstumsregulator und mittel für die wurzelbildung, die dieses jeweils als wirkstoff enthalten
EP1509599A1 (de)	2005-03-02	Aldehyd-dehydrogenase
US20050153412A1 (en)	2005-07-14	Aldehyde dehydrogenase II
AU2002328843A1 (en)	2003-03-03	Method for producing 2-keto-l-gulonic acid and vitamin c while using l-sorbose dehydrogenase and l-sorbosone dehydrogenase
RU2001118278A (ru)	2003-07-10	Получение аскорбиновой кислоты с помощью дрожжей
ES8800983A1 (es)	1987-12-16	Procedimiento para producir un microorganismo recombinante capaz de convertir glucosa u otro metabolico ordinario en acido 2-ceto-l-gulonico.
KR840004167A (ko)	1984-10-10	2-케토-l-굴론산의 제조방법
KR970043026A (ko)	1997-07-26	코리네박테리움 속의 신규 미생물 및 이를 이용한 2-케토-엘-굴론산의 제조방법