ATE322554T1 - Dns charakterisierung - Google Patents

Dns charakterisierung

Info

Publication number
ATE322554T1
ATE322554T1 AT98932421T AT98932421T ATE322554T1 AT E322554 T1 ATE322554 T1 AT E322554T1 AT 98932421 T AT98932421 T AT 98932421T AT 98932421 T AT98932421 T AT 98932421T AT E322554 T1 ATE322554 T1 AT E322554T1
Authority
AT
Austria
Prior art keywords
nucleic acid
stranded
nucleic acids
immobilized
cleaved
Prior art date
Application number
AT98932421T
Other languages
English (en)
Inventor
Guenter Schmidt
Andrew Hugin Thompson
Original Assignee
Xzillion Gmbh & Co Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xzillion Gmbh & Co Kg filed Critical Xzillion Gmbh & Co Kg
Application granted granted Critical
Publication of ATE322554T1 publication Critical patent/ATE322554T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AT98932421T 1997-07-11 1998-07-13 Dns charakterisierung ATE322554T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9714716.9A GB9714716D0 (en) 1997-07-11 1997-07-11 Characterising nucleic acids

Publications (1)

Publication Number Publication Date
ATE322554T1 true ATE322554T1 (de) 2006-04-15

Family

ID=10815765

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98932421T ATE322554T1 (de) 1997-07-11 1998-07-13 Dns charakterisierung

Country Status (7)

Country Link
US (1) US6297017B1 (de)
EP (1) EP0994969B1 (de)
AT (1) ATE322554T1 (de)
AU (1) AU8234998A (de)
DE (1) DE69834112D1 (de)
GB (1) GB9714716D0 (de)
WO (1) WO1999002727A1 (de)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1002128A1 (de) * 1997-07-11 2000-05-24 Brax Genomics Limited Charakterisierung von nukleinsäuren
WO1999002725A1 (en) * 1997-07-11 1999-01-21 Brax Group Limited Categorising nucleic acid
WO2001021840A2 (en) * 1999-09-23 2001-03-29 Gene Logic, Inc. Indexing populations
US7188306B1 (en) * 2001-02-28 2007-03-06 Xerox Corporation Swoopy text for connecting annotations in fluid documents
US9394332B2 (en) * 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
CN102344960B (zh) * 2002-09-06 2014-06-18 波士顿大学信托人 基因表达的定量
US7709262B2 (en) * 2004-02-18 2010-05-04 Trustees Of Boston University Method for detecting and quantifying rare mutations/polymorphisms
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
JP5738597B2 (ja) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
WO2010033200A2 (en) * 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3150724A1 (de) 2008-12-19 2017-04-05 President and Fellows of Harvard College Partikelunterstützte nukleinsäuresequenzierung
JP5791621B2 (ja) 2009-10-27 2015-10-07 プレジデント アンド フェローズ オブ ハーバード カレッジ 液滴生成技術
EP3597771A1 (de) 2009-11-25 2020-01-22 Gen9, Inc. Verfahren und vorrichtung für chipbasierte dna-fehlerreduktion
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2892818A1 (en) 2012-11-28 2014-06-05 Abwiz Bio, Inc. Preparation of gene-specific templates for the use in single primer amplification
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20200140929A (ko) 2013-02-08 2020-12-16 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
SG11201600853UA (en) 2013-08-05 2016-03-30 Twist Bioscience Corp De novo synthesized gene libraries
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
BR112016023625A2 (pt) 2014-04-10 2018-06-26 10X Genomics, Inc. dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos
EP3140428B1 (de) * 2014-05-08 2021-07-28 Fluidigm Corporation Verfahren zur markierung von nukleinsäuresequenzen
WO2015191815A1 (en) 2014-06-13 2015-12-17 Life Technologies Corporation Multiplex nucleic acid amplification
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
KR20170023979A (ko) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. 핵산 서열 조립을 위한 프로세스 및 시스템
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2015200893A2 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
BR112017008877A2 (pt) 2014-10-29 2018-07-03 10X Genomics Inc métodos e composições para sequenciamento de ácido nucleico-alvo
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
JP6769969B2 (ja) 2015-01-12 2020-10-14 １０エックス ジェノミクス， インコーポレイテッド 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー
WO2016115273A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016130578A1 (en) 2015-02-09 2016-08-18 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
EP3262407B1 (de) 2015-02-24 2023-08-30 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
MX2017010857A (es) 2015-02-24 2017-12-11 10X Genomics Inc Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana.
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3350314A4 (de) 2015-09-18 2019-02-06 Twist Bioscience Corporation Oligonukleinsäurevariantenbibliotheken variante und synthese davon
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
JP2018537414A (ja) 2015-10-13 2018-12-20 プレジデント アンド フェローズ オブ ハーバード カレッジ ゲルマイクロスフェアの作製及び使用のためのシステム及び方法
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
JP6954899B2 (ja) 2015-12-04 2021-10-27 １０エックス ゲノミクス，インコーポレイテッド 核酸の解析のための方法及び組成物
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
EP3500672A4 (de) 2016-08-22 2020-05-20 Twist Bioscience Corporation De-novo-synthetisierte nukleinsäure-bibliotheken
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB2559118B (en) * 2017-01-19 2021-07-07 Agency For Science Tech And Research Astarstar Method for normalizing a nucleic acid sample
EP4029939B1 (de) 2017-01-30 2023-06-28 10X Genomics, Inc. Verfahren und systeme für eine einzelzellbarcodierung auf tröpfchenbasis
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4556433A3 (de) 2017-02-22 2025-08-06 Twist Bioscience Corporation Nukleinsäurebasierte datenspeicherung
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
EP3625715A4 (de) 2017-05-19 2021-03-17 10X Genomics, Inc. Systeme und verfahren zur analyse von datensätzen
EP3445876B1 (de) 2017-05-26 2023-07-05 10X Genomics, Inc. Einzelzellenanalyse von transposasezugänglichem chromatin
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
WO2020139871A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
EP4034566A4 (de) 2019-09-23 2024-01-24 Twist Bioscience Corporation Variante nukleinsäurebibliotheken für crth2
JP2022548783A (ja) 2019-09-23 2022-11-21 ツイスト バイオサイエンス コーポレーション 単一ドメイン抗体のバリアント核酸ライブラリー
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
CN115428088A (zh) 2020-02-13 2022-12-02 10X基因组学有限公司 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法
WO2023102141A2 (en) * 2021-12-02 2023-06-08 Bio-Rad Laboratories, Inc. Use of dna:rna duplex fragmentation

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4705616A (en) * 1986-09-15 1987-11-10 Sepragen Corporation Electrophoresis-mass spectrometry probe
US5003059A (en) * 1988-06-20 1991-03-26 Genomyx, Inc. Determining DNA sequences by mass spectrometry
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
GB9210176D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
CA2140081C (en) 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
DE69621507T2 (de) * 1995-03-28 2003-01-09 Japan Science And Technology Corp., Kawaguchi Verfahren zur molekularen Indexierung von Genen unter Verwendung von Restriktionsenzymen
US5919626A (en) * 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces

Also Published As

Publication number Publication date
GB9714716D0 (en) 1997-09-17
WO1999002727A1 (en) 1999-01-21
US6297017B1 (en) 2001-10-02
EP0994969A1 (de) 2000-04-26
EP0994969B1 (de) 2006-04-05
AU8234998A (en) 1999-02-08
DE69834112D1 (de) 2006-05-18

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