ATE322688T1 - Verfahren zur bestimmung der nitrosylierung von proteinen - Google Patents

Verfahren zur bestimmung der nitrosylierung von proteinen

Info

Publication number
ATE322688T1
ATE322688T1 AT01983223T AT01983223T ATE322688T1 AT E322688 T1 ATE322688 T1 AT E322688T1 AT 01983223 T AT01983223 T AT 01983223T AT 01983223 T AT01983223 T AT 01983223T AT E322688 T1 ATE322688 T1 AT E322688T1
Authority
AT
Austria
Prior art keywords
proteins
nitrosylation
nitrosothiols
cysteines
detection
Prior art date
Application number
AT01983223T
Other languages
English (en)
Inventor
Samie Jaffrey
Christopher D Ferris
Solomon H Snyder
Paul Tempst
Original Assignee
Univ Johns Hopkins
Sloan Kettering Inst Cancer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Johns Hopkins, Sloan Kettering Inst Cancer filed Critical Univ Johns Hopkins
Application granted granted Critical
Publication of ATE322688T1 publication Critical patent/ATE322688T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G01N33/6815—Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y10T436/170769—N-Nitroso containing [e.g., nitrosamine, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y10T436/177692—Oxides of nitrogen
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT01983223T 2000-10-27 2001-10-29 Verfahren zur bestimmung der nitrosylierung von proteinen ATE322688T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US24409700P 2000-10-27 2000-10-27

Publications (1)

Publication Number Publication Date
ATE322688T1 true ATE322688T1 (de) 2006-04-15

Family

ID=22921362

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01983223T ATE322688T1 (de) 2000-10-27 2001-10-29 Verfahren zur bestimmung der nitrosylierung von proteinen

Country Status (6)

Country Link
US (2) US6806057B2 (de)
EP (1) EP1379877B1 (de)
AT (1) ATE322688T1 (de)
AU (1) AU2002214668A1 (de)
DE (1) DE60118621T2 (de)
WO (1) WO2002039119A2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005081622A1 (en) * 2004-02-17 2005-09-09 Pharmacia & Upjohn Company Llc Methods and compositions for the detection of nitric oxide
WO2005101019A2 (en) * 2004-04-12 2005-10-27 University Of Vermont And State Agricultural College Detection of nitrosylated proteins
US7507585B2 (en) * 2005-01-10 2009-03-24 Whalen Erin J Methods and systems for indentifying and monitoring S-nitrosothiols in biological samples
WO2007087365A2 (en) * 2006-01-25 2007-08-02 University Of Vermont And State Agricultural College Detection of glutathionylated proteins
ITPI20060093A1 (it) * 2006-07-21 2008-01-22 Univ Pisa Procedimento per la determinazione di s-nitrosotioli in fluidi biologici
CA2684948A1 (en) * 2007-05-15 2008-11-27 Joseph Loscalzo Identification of oxidatively modified peptide sequences in the proteome
WO2008154012A2 (en) * 2007-06-11 2008-12-18 University Of Vermont And State Agricultural College Treatments involving glutaredoxins and similar agents
GB0716328D0 (en) * 2007-08-21 2007-10-03 Univ Bath Detection and functionalisation of S-nitrosylated polypeptides
CN101893634B (zh) * 2009-05-20 2013-08-28 中国科学院生物物理研究所 特异检测蛋白质或多肽半胱氨酸巯基修饰的方法及其用途
WO2011022780A1 (en) * 2009-08-27 2011-03-03 South Eastern Sydney And Illawarra Area Health Service Methods for the diagnosis and prognosis of autoimmune disease
US9835629B2 (en) 2012-05-18 2017-12-05 Pierce Biotechnology, Inc. Methods and reagents for biomolecule labeling, enrichment and gentle elution
US9937144B2 (en) 2013-01-30 2018-04-10 The Johns Hopkins University Treatment of drug abuse by preventing GAPDH nitrosylation
JP6296533B2 (ja) * 2013-09-03 2018-03-20 株式会社 資生堂 システイン及びシスチンの定量方法
US10072097B2 (en) 2014-08-04 2018-09-11 Academia Sinica Compositions and methods for detection of protein S-nitrosylation and oxidation
CN108469527B (zh) * 2018-03-19 2021-02-02 山西大学 一种蛋白质巯基化修饰检测的方法
CN110850102A (zh) * 2019-12-09 2020-02-28 安阳师范学院 一种特异肽段质谱分析样本的制备方法
CN117030899A (zh) * 2023-08-28 2023-11-10 中信湘雅生殖与遗传专科医院有限公司 蛋白样品的处理方法及蛋白组分析方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4281061A (en) * 1979-07-27 1981-07-28 Syva Company Double antibody for enhanced sensitivity in immunoassay
DE3540076A1 (de) * 1985-11-12 1987-05-14 Boehringer Mannheim Gmbh Verfahren zur stabilisierung von creatinkinase
WO1997033989A1 (en) * 1996-03-15 1997-09-18 The General Hospital Corporation TRANSGENIC ANIMALS HAVING A DISRUPTED eNOS GENE AND USE THEREOF
US6417180B1 (en) * 1998-10-07 2002-07-09 University Of Massachusetts Zinc finger-reactive antimicrobial compounds
EP1311858B1 (de) * 2000-05-05 2006-11-02 Purdue Research Foundation Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen

Also Published As

Publication number Publication date
WO2002039119A2 (en) 2002-05-16
WO2002039119A3 (en) 2003-10-30
US7001738B2 (en) 2006-02-21
EP1379877A2 (de) 2004-01-14
US6806057B2 (en) 2004-10-19
DE60118621D1 (de) 2006-05-18
EP1379877B1 (de) 2006-04-05
AU2002214668A1 (en) 2002-05-21
US20050026227A1 (en) 2005-02-03
DE60118621T2 (de) 2007-05-03
US20020102744A1 (en) 2002-08-01

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