ATE325880T1 - Herstellung eines rekombinanten proteins in prokaryotischen wirtszellen durch kodonsoptimisierung - Google Patents

Herstellung eines rekombinanten proteins in prokaryotischen wirtszellen durch kodonsoptimisierung

Info

Publication number
ATE325880T1
ATE325880T1 AT01955373T AT01955373T ATE325880T1 AT E325880 T1 ATE325880 T1 AT E325880T1 AT 01955373 T AT01955373 T AT 01955373T AT 01955373 T AT01955373 T AT 01955373T AT E325880 T1 ATE325880 T1 AT E325880T1
Authority
AT
Austria
Prior art keywords
host cells
prokaryotic host
production
recombinant protein
codon optimization
Prior art date
Application number
AT01955373T
Other languages
English (en)
Inventor
Rolf-Guenther Werner
Klaus Bergemann
Friedrich Goetz
Andreas Peschel
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Application granted granted Critical
Publication of ATE325880T1 publication Critical patent/ATE325880T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT01955373T 2000-07-27 2001-07-21 Herstellung eines rekombinanten proteins in prokaryotischen wirtszellen durch kodonsoptimisierung ATE325880T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10037111A DE10037111A1 (de) 2000-07-27 2000-07-27 Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle

Publications (1)

Publication Number Publication Date
ATE325880T1 true ATE325880T1 (de) 2006-06-15

Family

ID=7650734

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01955373T ATE325880T1 (de) 2000-07-27 2001-07-21 Herstellung eines rekombinanten proteins in prokaryotischen wirtszellen durch kodonsoptimisierung

Country Status (11)

Country Link
EP (1) EP1307569B1 (de)
JP (1) JP5044085B2 (de)
CN (2) CN1444655A (de)
AT (1) ATE325880T1 (de)
AU (1) AU2001277554A1 (de)
CA (1) CA2414978C (de)
DE (2) DE10037111A1 (de)
DK (1) DK1307569T3 (de)
ES (1) ES2264700T3 (de)
MX (1) MXPA03000427A (de)
WO (1) WO2002010411A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001249170A1 (en) * 2000-03-13 2001-09-24 Aptagen Method for modifying a nucleic acid
US8551469B2 (en) 2001-02-28 2013-10-08 Superlab Far East Limited Treatment of tumors and viral diseases with recombinant interferon alpha
JP4124639B2 (ja) * 2002-12-17 2008-07-23 株式会社日本触媒 大腸菌を用いたｓ−ヒドロキシニトリルリアーゼの製造方法
WO2006134497A2 (en) * 2005-03-09 2006-12-21 Guangwen Wei Uses of recombinant super-compound interferons
US8569015B2 (en) 2006-05-30 2013-10-29 Pfenex Inc. RPA optimization
JP4886654B2 (ja) * 2007-10-29 2012-02-29 日本ケミカルリサーチ株式会社 ヒト成長ホルモンの効率化された製造方法
CN102286101B (zh) * 2011-08-08 2013-05-08 常州亚当生物技术有限公司 抗VEGF单克隆抗体Fab片段Vasculizumab及其应用
BR112014025693B1 (pt) * 2012-04-17 2021-12-07 F. Hoffmann-La Roche Ag Método de produção recombinante de um polipeptídeo em uma célula bacteriana
CN107723287B (zh) * 2016-08-12 2021-07-06 中国科学院天津工业生物技术研究所 一种增强丝蛋白生产制备的表达系统
CN108456703B (zh) * 2017-02-20 2022-01-14 复旦大学 一种异源表达埃博霉素的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU618640B2 (en) * 1988-11-11 1992-01-02 Boehringer Mannheim Gmbh Process for the expression of a recombinant gene
JPH05284973A (ja) * 1992-04-13 1993-11-02 Kurita Water Ind Ltd 組換プラスミドおよびこれをベクターとして用いる異種蛋白質の分泌生産方法
JP3556965B2 (ja) * 1993-11-08 2004-08-25 天野エンザイム株式会社 糸状菌および酵母で使用可能なポリペプチド分泌発現用プラスミドおよびそれを用いたポリペプチドの製造法
WO1998035029A1 (en) * 1997-02-07 1998-08-13 Vanderbilt University Synthetic genes for recombinant mycobacterium proteins
AUPP807899A0 (en) * 1999-01-08 1999-02-04 University Of Queensland, The Codon utilization
JP2000175687A (ja) * 1998-12-16 2000-06-27 Japan Science & Technology Corp ネオカルチノスタチン・アポタンパク質合成遺伝子
WO2000044926A1 (en) * 1999-01-27 2000-08-03 Stratagene High level expression of a heterologous protein having rare codons

Also Published As

Publication number Publication date
DE60119535D1 (de) 2006-06-14
WO2002010411A3 (en) 2002-07-18
CN1444655A (zh) 2003-09-24
CN101525628A (zh) 2009-09-09
MXPA03000427A (es) 2003-10-06
DE60119535T2 (de) 2006-09-07
CN101525628B (zh) 2011-08-24
CA2414978A1 (en) 2002-02-07
EP1307569B1 (de) 2006-05-10
CA2414978C (en) 2009-02-24
EP1307569A2 (de) 2003-05-07
JP5044085B2 (ja) 2012-10-10
DE10037111A1 (de) 2002-02-07
WO2002010411A2 (en) 2002-02-07
JP2004504847A (ja) 2004-02-19
DK1307569T3 (da) 2006-09-04
ES2264700T3 (es) 2007-01-16
AU2001277554A1 (en) 2002-02-13

Similar Documents

Publication Publication Date Title
SG162834A1 (en) Refolding of recombinant proteins
TW200732472A (en) Method for the recombinant expression of a polypeptide
ATE374828T1 (de) Expressionsvektor zur antibiotikafreien expression
WO2005014642A3 (en) Novel multifunctional cytokines
ATE325880T1 (de) Herstellung eines rekombinanten proteins in prokaryotischen wirtszellen durch kodonsoptimisierung
AU2002234863A1 (en) Denaturat stable and/or protease resistant, chaperone-like oligomeric proteins, polynucleotides encoding same and their uses
BRPI0207394B8 (pt) dna codificando uma proteína de fusão proteína codificada por um dna, plasmídeo, micro-organismo hospedeiro, processo para a produção fermentativa de uma proteína de fusão e processo para a produçao de insulina
DK1874932T3 (da) Produktion af rekombinante proteiner ved autoproteolytisk spaltning af et fusionsprotein
UA97628C2 (ru) Способ получения fc-фрагмента иммуноглобулина, свободного от начального метионинового остатка, в массовом масштабе
WO2002081709A3 (en) Modified hpv e6 and e7 genes and proteins useful for vaccination
PE20020857A1 (es) Proteinas de fusion cuya parte del n-terminal es un derivado de hirudina para la produccion de proteinas recombinantes a traves de la secresion por levaduras
ATE540981T1 (de) Lösliche fusionsproteine enthaltend heterologe polypeptide
EP1530637A4 (de) Subtilisin-carlsberg-proteine mit verminderter immunogenität
ATE493497T1 (de) Verfahren zur herstellung von oligopeptiden
DE60326449D1 (de) Chimere human papilloma virus 16 l1 proteine, welche ein l2 peptid enthalten, damit hergestellte virus-ähnliche partikel und methode zur herstellung der partikel
WO2006042719A3 (de) Polypeptid mit phytaseaktivität und dieses codierende nucleotidsequenz
ATE295890T1 (de) Vektor zur expression von n-terminal verlängerten proteinen in hefezellen
ATE466874T1 (de) Mit scytovirin der domäne 1 verwandte polypeptide
TW200734351A (en) Recombinant production of heparin binding proteins
DK0422217T3 (da) Human, rekombinant, placental ribonucleaseinhibitor og fremgangsmåde til produktion
NZ501124A (en) cDNA clones of the RSPaV viral strain and associated polypeptides and antibodies thereof
WO2006090282A3 (en) Recombinant inhibitor proteins of an hk14 protease and use thereof
DE602004004796D1 (de) Expressionsvektoren, transformierte wirtszellen und fermentationsverfahren zur herstellung rekombinanter polypeptide
SE0303093D0 (sv) Expression vector, host cell and method for producing fusion proteins
WO2001096578A3 (en) Protein production in aureobasidium pullulans

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1307569

Country of ref document: EP