DK1307569T3 - Fremstilling af rekombinant protein i en prokaryot værtscelle ved forbedret codonanvendelse - Google Patents

Fremstilling af rekombinant protein i en prokaryot værtscelle ved forbedret codonanvendelse

Info

Publication number
DK1307569T3
DK1307569T3 DK01955373T DK01955373T DK1307569T3 DK 1307569 T3 DK1307569 T3 DK 1307569T3 DK 01955373 T DK01955373 T DK 01955373T DK 01955373 T DK01955373 T DK 01955373T DK 1307569 T3 DK1307569 T3 DK 1307569T3
Authority
DK
Denmark
Prior art keywords
host cell
preparation
prokaryotic host
recombinant protein
codon usage
Prior art date
Application number
DK01955373T
Other languages
English (en)
Inventor
Klaus Bergemann
Andreas Peschel
Rolf-Guenther Werner
Friedrich Goetz
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Application granted granted Critical
Publication of DK1307569T3 publication Critical patent/DK1307569T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK01955373T 2000-07-27 2001-07-21 Fremstilling af rekombinant protein i en prokaryot værtscelle ved forbedret codonanvendelse DK1307569T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10037111A DE10037111A1 (de) 2000-07-27 2000-07-27 Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle

Publications (1)

Publication Number Publication Date
DK1307569T3 true DK1307569T3 (da) 2006-09-04

Family

ID=7650734

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01955373T DK1307569T3 (da) 2000-07-27 2001-07-21 Fremstilling af rekombinant protein i en prokaryot værtscelle ved forbedret codonanvendelse

Country Status (11)

Country Link
EP (1) EP1307569B1 (da)
JP (1) JP5044085B2 (da)
CN (2) CN1444655A (da)
AT (1) ATE325880T1 (da)
AU (1) AU2001277554A1 (da)
CA (1) CA2414978C (da)
DE (2) DE10037111A1 (da)
DK (1) DK1307569T3 (da)
ES (1) ES2264700T3 (da)
MX (1) MXPA03000427A (da)
WO (1) WO2002010411A2 (da)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001249170A1 (en) * 2000-03-13 2001-09-24 Aptagen Method for modifying a nucleic acid
US8551469B2 (en) 2001-02-28 2013-10-08 Superlab Far East Limited Treatment of tumors and viral diseases with recombinant interferon alpha
JP4124639B2 (ja) * 2002-12-17 2008-07-23 株式会社日本触媒 大腸菌を用いたｓ−ヒドロキシニトリルリアーゼの製造方法
AU2006257286B2 (en) * 2005-03-09 2012-08-16 Superlab Far East Limited Uses of recombinant super-compound interferons
WO2008027099A2 (en) 2006-05-30 2008-03-06 Dow Global Technologies Inc. Rpa optimization
JP4886654B2 (ja) * 2007-10-29 2012-02-29 日本ケミカルリサーチ株式会社 ヒト成長ホルモンの効率化された製造方法
CN102286101B (zh) * 2011-08-08 2013-05-08 常州亚当生物技术有限公司 抗VEGF单克隆抗体Fab片段Vasculizumab及其应用
JP6224077B2 (ja) 2012-04-17 2017-11-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改変された核酸を用いてポリペプチドを発現させるための方法
CN107723287B (zh) * 2016-08-12 2021-07-06 中国科学院天津工业生物技术研究所 一种增强丝蛋白生产制备的表达系统
CN108456703B (zh) * 2017-02-20 2022-01-14 复旦大学 一种异源表达埃博霉素的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU618640B2 (en) * 1988-11-11 1992-01-02 Boehringer Mannheim Gmbh Process for the expression of a recombinant gene
JPH05284973A (ja) * 1992-04-13 1993-11-02 Kurita Water Ind Ltd 組換プラスミドおよびこれをベクターとして用いる異種蛋白質の分泌生産方法
JP3556965B2 (ja) * 1993-11-08 2004-08-25 天野エンザイム株式会社 糸状菌および酵母で使用可能なポリペプチド分泌発現用プラスミドおよびそれを用いたポリペプチドの製造法
WO1998035029A1 (en) * 1997-02-07 1998-08-13 Vanderbilt University Synthetic genes for recombinant mycobacterium proteins
AUPP807899A0 (en) * 1999-01-08 1999-02-04 University Of Queensland, The Codon utilization
JP2000175687A (ja) * 1998-12-16 2000-06-27 Japan Science & Technology Corp ネオカルチノスタチン・アポタンパク質合成遺伝子
ATE434051T1 (de) * 1999-01-27 2009-07-15 Stratagene California Hohe expression eines heterologen proteins mit seltenen codons

Also Published As

Publication number Publication date
ES2264700T3 (es) 2007-01-16
CA2414978C (en) 2009-02-24
CN101525628A (zh) 2009-09-09
JP5044085B2 (ja) 2012-10-10
WO2002010411A2 (en) 2002-02-07
ATE325880T1 (de) 2006-06-15
EP1307569A2 (en) 2003-05-07
AU2001277554A1 (en) 2002-02-13
DE60119535T2 (de) 2006-09-07
CN101525628B (zh) 2011-08-24
JP2004504847A (ja) 2004-02-19
CN1444655A (zh) 2003-09-24
EP1307569B1 (en) 2006-05-10
MXPA03000427A (es) 2003-10-06
WO2002010411A3 (en) 2002-07-18
CA2414978A1 (en) 2002-02-07
DE10037111A1 (de) 2002-02-07
DE60119535D1 (de) 2006-06-14

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