ATE306562T1 - Verfahren zur analyse von wiederkehrenden pcr- podukten, das auf der analyse von dns- schmelzkurven basiert - Google Patents

Verfahren zur analyse von wiederkehrenden pcr- podukten, das auf der analyse von dns- schmelzkurven basiert

Info

Publication number
ATE306562T1
ATE306562T1 AT01126930T AT01126930T ATE306562T1 AT E306562 T1 ATE306562 T1 AT E306562T1 AT 01126930 T AT01126930 T AT 01126930T AT 01126930 T AT01126930 T AT 01126930T AT E306562 T1 ATE306562 T1 AT E306562T1
Authority
AT
Austria
Prior art keywords
analysis
recurrent
analyzing
pcr products
products based
Prior art date
Application number
AT01126930T
Other languages
English (en)
Inventor
Wolfgang Dr Dietmaier
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE306562T1 publication Critical patent/ATE306562T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT01126930T 2000-11-15 2001-11-13 Verfahren zur analyse von wiederkehrenden pcr- podukten, das auf der analyse von dns- schmelzkurven basiert ATE306562T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP00124897 2000-11-15

Publications (1)

Publication Number Publication Date
ATE306562T1 true ATE306562T1 (de) 2005-10-15

Family

ID=8170382

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01126930T ATE306562T1 (de) 2000-11-15 2001-11-13 Verfahren zur analyse von wiederkehrenden pcr- podukten, das auf der analyse von dns- schmelzkurven basiert

Country Status (4)

Country Link
US (1) US6664064B1 (de)
JP (1) JP3844996B2 (de)
AT (1) ATE306562T1 (de)
DE (1) DE60113945T2 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004031408A1 (en) 2002-09-30 2004-04-15 F.Hoffmann-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
DK1567668T3 (da) * 2002-12-06 2009-08-17 Innogenetics Nv Detektion, identificering og differentiering af eubakterielle taxa ved anvendelse af et hybridiseringsassay
JP4571786B2 (ja) * 2003-06-10 2010-10-27 則夫 清水 標的核酸の検出法
US20050255483A1 (en) * 2004-05-14 2005-11-17 Stratagene California System and method for smoothing melting curve data
WO2006070666A1 (ja) * 2004-12-28 2006-07-06 Takara Bio Inc. 遺伝子多型の同時検出方法
US7977108B2 (en) * 2005-07-25 2011-07-12 Roche Molecular Systems, Inc. Method for detecting a mutation in a repetitive nucleic acid sequence
WO2007115059A2 (en) 2006-03-29 2007-10-11 Merial Limited Vaccine against streptococci
US9404148B2 (en) * 2007-10-22 2016-08-02 Lgc Limited Oligonucleotides and uses thereof
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
JP5509075B2 (ja) * 2008-12-16 2014-06-04 アークレイ株式会社 核酸増幅のコントロールの検出方法およびその用途
JP5917248B2 (ja) * 2011-05-09 2016-05-11 アークレイ株式会社 ＨＧＦ遺伝子のｐｏｌｙＡｒｅｐｅａｔ数変異多型検出用プローブおよびその用途
CN102409088B (zh) * 2011-09-22 2014-11-12 郭奇伟 一种基因拷贝数变异的检测方法
EP2766711B1 (de) 2011-10-14 2017-12-13 Becton, Dickinson and Company Thermisches zyklisierungsverfahren beruhend auf rechteckwellen
US10294529B2 (en) 2012-04-10 2019-05-21 Life Sciences Research Partners Vzw Microsatellite instability markers in detection of cancer
US10633707B2 (en) 2012-04-10 2020-04-28 Vib Vzw Markers for detecting microsatellite instability in cancer and determining synthetic lethality with inhibition of the DNA base excision repair pathway
CN114058704A (zh) * 2012-04-10 2022-02-18 非营利性组织佛兰芒综合大学生物技术研究所 用于检测癌症中的微卫星不稳定性和dna碱基切除修复途径抑制的合成致死性的新标记
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
KR102648647B1 (ko) * 2015-09-22 2024-03-18 비오까르띠 엔브이 짧은 호모폴리머릭 반복서열의 개선된 검출법
US11332781B2 (en) 2016-06-23 2022-05-17 Canon U.S.A., Inc. Fitting melting curve data to determine copy number variation
CN110621784B (zh) * 2017-03-24 2024-06-11 海阳生物材料有限公司 使用双功能pna探针诊断微卫星不稳定性的方法和诊断微卫星不稳定性的试剂盒
DE102018213587A1 (de) 2018-08-13 2020-02-13 Robert Bosch Gmbh Verfahren zum Bestimmen einer Spezifität einer Echtzeit-Polymerase-Kettenreaktion mit einer Schmelzkurvenanalyse

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5582979A (en) * 1989-04-21 1996-12-10 Marshfield Clinic Length polymorphisms in (dC-dA)n.(dG-dT)n sequences and method of using the same
US5075217A (en) * 1989-04-21 1991-12-24 Marshfield Clinic Length polymorphisms in (dC-dA)n ·(dG-dT)n sequences
US6218119B1 (en) * 1996-01-16 2001-04-17 Keygene, N. V. Amplification of simple sequence repeats
ATE229081T1 (de) * 1996-01-26 2002-12-15 Abbott Lab Verfahren zur analyse von nukleinsäure- wiederholungssequenzen
US6238866B1 (en) * 1996-04-16 2001-05-29 The United States Of America As Represented By The Secretary Of The Army Detector for nucleic acid typing and methods of using the same
AUPO229596A0 (en) * 1996-09-12 1996-10-03 Varian Australia Pty Ltd Method for analysis of DNA
GB2333597B (en) * 1996-10-29 2001-04-18 Univ Nebraska At Lincoln Method for detecting point mutations in dna utilizing fluorescence energy transfer
EP0896634A1 (de) * 1996-11-29 1999-02-17 Amersham Pharmacia Biotech UK Limited Verfahren zur bestimmung der länge von tandem-repeat-sequenzen
AUPO882497A0 (en) * 1997-08-29 1997-09-18 Grains Research & Development Corporation A method of genotyping
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
EP1207210B1 (de) * 2000-11-15 2005-10-12 Roche Diagnostics GmbH Verfahren zur Analyse von Wiederkehrenden PCR-Podukten, das auf der Analyse von DNS-Schmelzkurven basiert
JP2003093075A (ja) * 2001-09-26 2003-04-02 Adgene Co Ltd 電気抵抗を利用した核酸変異検出法

Also Published As

Publication number Publication date
US6664064B1 (en) 2003-12-16
DE60113945D1 (de) 2005-11-17
DE60113945T2 (de) 2006-06-29
JP2002191384A (ja) 2002-07-09
JP3844996B2 (ja) 2006-11-15

Similar Documents

Publication Publication Date Title
ATE306562T1 (de) Verfahren zur analyse von wiederkehrenden pcr- podukten, das auf der analyse von dns- schmelzkurven basiert
US9890375B2 (en) Isolated oligonucleotide and use thereof in nucleic acid sequencing
US10081825B2 (en) Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
ATE305054T1 (de) Verfahren und mittel zur analyse der nukleotidsequenz von nukleinsäuren
ES2159635T3 (es) Secuenciado de adn por ligazon y escindido por etapas.
FI3872187T3 (fi) Koostumuksia ja menetelmiä näytteiden identifioinnin parantamiseksi indeksoiduissa nukleiinihappokirjastoissa
ATE343586T1 (de) Verfahren zur verwendung einer universellen bibliothek von peptid-nukleinsäuren zur optimierung der dns-hybridisierung
ATE185843T1 (de) Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension
WO1996006187A1 (en) Nucleotide sequencing method
ATE521717T1 (de) Parallele nukleinsäuresequenzierungsverfahren
DE69931608D1 (de) Verfahren zur bestimmung von dna-längenpolymorphismen
DE60005309D1 (de) Methode zur sequenzierung von polynukleotiden
US20090087834A1 (en) Method for characterising polynucleotides
ATE395435T1 (de) Verfahren zur sequenzierung von dna
DE69937447D1 (de) Verfahren zur erkennung von nukleinsäuren
ATE431428T1 (de) Ein verfahren zur analyse von polynukleotiden
WO2004046344A3 (en) Polynucleotide sequence detection assays
ATE449868T1 (de) Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen
US10590453B2 (en) Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
DE60125054D1 (de) Verfahren zur amplifikation von nukleinsäuren, das mittel zur strangtrennung und universelle sequenzmarker bereitstellt
EP1323835A3 (de) Methode zur Bestimmung einer Biospezies in einer Testprobe und dafür verwendeter Testsatz
DE60237204D1 (de) Verfahren und sequenzen zur determinierten nukleinsäurehybridisierung
CN118703641A (zh) 一种tlamp法检测猪源性成分的引物组及方法
KR20210009224A (ko) 절단효소 인식부위의 미러링 결합에 의한 표적핵산의 검출 방법 및 핵산 검출용 조성물
JPH09140400A (ja) 塩基配列決定方法

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties