ATE329061T1 - Verfahren zur reduzierung unspezifischer amplifikation bei pcr - Google Patents

Verfahren zur reduzierung unspezifischer amplifikation bei pcr

Info

Publication number: ATE329061T1
Authority: AT; Austria
Prior art keywords: sorbitol; specific amplification; dmso; sample; reducing non
Prior art date: 2001-10-31

Application number

AT02802916T

Other languages

English (en)

Inventor

Ian J Mclaughlin

Sulekha Rao Coticone

Will Bloch

Original Assignee

Applera Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-10-31

Filing date

2002-07-30

Publication date

2006-06-15

2002-07-30 Application filed by Applera Corp filed Critical Applera Corp

2006-06-15 Application granted granted Critical

2006-06-15 Publication of ATE329061T1 publication Critical patent/ATE329061T1/de

Links

230000003321 amplification Effects 0.000 title abstract 6
238000003199 nucleic acid amplification method Methods 0.000 title abstract 6
238000000034 method Methods 0.000 title abstract 3
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 abstract 9
FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 abstract 6
FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 abstract 6
239000000600 sorbitol Substances 0.000 abstract 6
102000004190 Enzymes Human genes 0.000 abstract 2
108090000790 Enzymes Proteins 0.000 abstract 2
108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
108020004414 DNA Proteins 0.000 abstract 1
108020001027 Ribosomal DNA Proteins 0.000 abstract 1
230000000694 effects Effects 0.000 abstract 1
238000011534 incubation Methods 0.000 abstract 1
239000000203 mixture Substances 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
238000003752 polymerase chain reaction Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Biophysics (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Amplifiers (AREA)

AT02802916T 2001-10-31 2002-07-30 Verfahren zur reduzierung unspezifischer amplifikation bei pcr ATE329061T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US09/998,887 US6783940B2 (en)	2001-10-31	2001-10-31	Method of reducing non-specific amplification in PCR

Publications (1)

Publication Number	Publication Date
ATE329061T1 true ATE329061T1 (de)	2006-06-15

Family

ID=25545644

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT02802916T ATE329061T1 (de)	2001-10-31	2002-07-30	Verfahren zur reduzierung unspezifischer amplifikation bei pcr

Country Status (8)

Country	Link
US (1)	US6783940B2 (de)
EP (1)	EP1446508B1 (de)
JP (1)	JP2005512572A (de)
AT (1)	ATE329061T1 (de)
AU (1)	AU2002365068A1 (de)
CA (1)	CA2464885A1 (de)
DE (1)	DE60212175T2 (de)
WO (1)	WO2003054209A2 (de)

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US20070298423A1 (en) *	2000-03-24	2007-12-27	Eppendorf Array Technologies Sa (Eat)	Identification of multiple biological (micro) organisms by specific amplification and detection of their nucleotide sequences on arrays
US20080085515A1 (en) *	2000-03-24	2008-04-10	Eppendorf Array Technologies Sa (Eat)	Identification of multiple biological (micro) organisms by detection of their nucleotide sequences on arrays
US6841349B2 (en) *	2001-05-07	2005-01-11	Applera Corporation Applied Biosystems Group	Methods for the reduction of stutter in microsatellite amplification
US7354742B2 (en) *	2002-02-22	2008-04-08	Ortho-Mcneil Pharmaceutical, Inc.	Method for generating amplified RNA
EP1403385A1 (de) *	2002-09-27	2004-03-31	Institut National De La Sante Et De La Recherche Medicale (Inserm)	Methode zur Darstellung der Replikation von HBV und zum Testen der Sensibilität von Wirkstoffen
KR100880516B1 (ko) *	2003-10-16	2009-01-28	써드 웨이브 테크놀로지스, 아이앤씨.	체액에서 핵산의 직접 검출법
EP2042612B1 (de) *	2004-01-23	2010-12-15	bioMerieux, Inc.	Neuartiges Nukleotidgemisch für eine verbesserte Nukleinsäurenamplifikationsleistung
EP1718743B1 (de) *	2004-02-04	2009-08-26	QIAGEN North American Holdings, Inc.	Durch gefrierschutzprotein verbesserte nukleinsäureamplifikation
EP1766061B1 (de) *	2004-05-20	2013-07-17	Quest Diagnostics Investments Incorporated	Vergleichende einfachmarkierung-hybridisierung
CA2586246A1 (en) *	2004-11-03	2006-05-11	Third Wave Technologies, Inc.	Single step nucleic acid detection assay employing reverse transcription, polymerase chain reaction and endonuclease cleavage
JP2006262777A (ja) *	2005-03-24	2006-10-05	Japan Science & Technology Agency	夾雑物を含む試料を用いた核酸合成方法及び添加剤
WO2006119419A2 (en) *	2005-05-03	2006-11-09	Geunsook Jeon	Materials and kits for use in hot-start pcr, and methods of amplifying nucleic acids in a polymerase chain reaction
US20070048757A1 (en) *	2005-05-31	2007-03-01	Applera Corporation	Methods for characterizing cells using amplified micro rnas
US8097414B2 (en)	2005-11-25	2012-01-17	K. K. Dnaform	Method for detecting and amplifying nucleic acid
WO2007115059A2 (en)	2006-03-29	2007-10-11	Merial Limited	Vaccine against streptococci
US7772390B1 (en)	2006-07-18	2010-08-10	The Regents Of The University Of California	Lipid mediated nucleic acid synthesis
KR100773561B1 (ko) *	2006-11-07	2007-11-05	삼성전자주식회사	다중 ｐｃｒ에서 비특이적 증폭을 감소시키는 장치 및 방법
US7507539B2 (en) *	2007-07-30	2009-03-24	Quest Diagnostics Investments Incorporated	Substractive single label comparative hybridization
CN102301000B (zh)	2008-03-15	2015-04-29	豪洛捷公司	核酸分子扩增反应的分析组分及方法
EP3733871A1 (de)	2008-05-27	2020-11-04	Dako Denmark A/S	Zusammensetzungen und verfahren zur erkennung von chromosomalen abweichungen mit neuen hybridisierungspuffern
CA2750820A1 (en) *	2009-01-27	2010-08-05	Qiagen Gaithersburg	Thermophilic helicase dependent amplification technology with endpoint homogenous fluorescent detection
EP2401396B1 (de)	2009-02-26	2016-11-23	Dako Denmark A/S	Verfahren zur durchführung eines stringenten waschschritts bei hybridisierungsanwendungen
WO2013046033A1 (en)	2011-09-30	2013-04-04	Dako Denmark A/S	Hybridization compositions and methods using formamide
EP2768974B1 (de)	2011-10-21	2017-07-19	Dako Denmark A/S	Hybridisierungszusammensetzungen und -verfahren
SG10201706147YA (en) *	2012-12-18	2017-08-30	Merial Inc	High resolution melt genotyping of ibv, csfv and ndv
US9630182B2 (en) *	2013-12-04	2017-04-25	Leidos Innovations Technology, Inc.	Non-contact infrared thermocycling
WO2019028430A1 (en) *	2017-08-03	2019-02-07	The Trustees Of Columbia University In The City Of New York	OPTICAL SUPER-MULTIPLEXING BY POLYYNESES

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GB9024005D0 (en) *	1990-11-05	1990-12-19	British Bio Technology	Process for amplifying nucleic acid
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DE4411588C1 (de)	1994-03-30	1995-09-28	Deutsches Rheuma Forschungszen	Puffer für DNA- und RNA-Polymerase-Reaktionen
US5580728A (en)	1994-06-17	1996-12-03	Perlin; Mark W.	Method and system for genotyping
US5545539A (en)	1994-10-18	1996-08-13	Genzyme Corporation	Method for nucleotide sequence amplification
US6077664A (en) *	1995-06-07	2000-06-20	Promega Corporation	Thermophilic DNA polymerases from Thermotoga neapolitana
US6156512A (en)	1996-05-23	2000-12-05	Promega Corp	Allelic ladders for short tandem repeat loci
US6787305B1 (en)	1998-03-13	2004-09-07	Invitrogen Corporation	Compositions and methods for enhanced synthesis of nucleic acid molecules
JP4438110B2 (ja) *	1998-07-01	2010-03-24	東ソー株式会社	標的核酸の定量方法

2001
- 2001-10-31 US US09/998,887 patent/US6783940B2/en not_active Expired - Lifetime
2002
- 2002-07-30 EP EP02802916A patent/EP1446508B1/de not_active Expired - Lifetime
- 2002-07-30 JP JP2003554910A patent/JP2005512572A/ja not_active Withdrawn
- 2002-07-30 DE DE60212175T patent/DE60212175T2/de not_active Expired - Lifetime
- 2002-07-30 AU AU2002365068A patent/AU2002365068A1/en not_active Abandoned
- 2002-07-30 WO PCT/US2002/024036 patent/WO2003054209A2/en not_active Ceased
- 2002-07-30 AT AT02802916T patent/ATE329061T1/de not_active IP Right Cessation
- 2002-07-30 CA CA002464885A patent/CA2464885A1/en not_active Abandoned

Also Published As

Publication number	Publication date
EP1446508A2 (de)	2004-08-18
WO2003054209A3 (en)	2003-12-04
US6783940B2 (en)	2004-08-31
JP2005512572A (ja)	2005-05-12
AU2002365068A1 (en)	2003-07-09
CA2464885A1 (en)	2003-07-03
DE60212175T2 (de)	2006-10-12
EP1446508A4 (de)	2005-03-09
DE60212175D1 (de)	2006-07-20
WO2003054209A2 (en)	2003-07-03
US20030104395A1 (en)	2003-06-05
EP1446508B1 (de)	2006-06-07

Legal Events

Date	Code	Title	Description
2006-12-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
ATE329061T1 (de)	2006-06-15	Verfahren zur reduzierung unspezifischer amplifikation bei pcr
DE60220025D1 (de)	2007-06-21	Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
WO2002072773A3 (en)	2003-12-18	Methods and compositions for amplification of rna sequences
DE60143960D1 (de)	2011-03-10	Äuren
US8623602B2 (en)	2014-01-07	Lysis and reverse transcription for MRNA quantification
WO2005098042A3 (en)	2006-08-24	Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity
KR970701787A (ko)	1997-04-12	이온성 세제에 의한 효소 개재 반응의 억제를 방지하는 방법(Method for Suppressing Inhibition of Enzyme-Mediated Reactions By Ionic Detergents)
DE59509937D1 (de)	2002-01-24	Verfahren zur Quantifizierung von genomischer DNA
WO2006076017A3 (en)	2007-05-31	Methods and kits for identifying target nucleotides in mixed populations
DE60143314D1 (de)	2010-12-02	Estimmung der präsenz von cryptosporidium organismen in einer testprobe
US8153401B2 (en)	2012-04-10	Method for direct amplification from crude nucleic acid samples
WO2002064842A3 (en)	2003-12-18	Quantitative epstein barr virus pcr rapid assay
WO2004050911A3 (en)	2004-08-19	Method for lowering both sequence variations and increase of base lines effects in diagnostic hybridisation assay, assay for performing such a method and probe for use in the assay
Zwirglmaier et al.	2021	Rapid detection of SARS-CoV-2 by pulse-controlled amplification (PCA)
US20080233587A1 (en)	2008-09-25	Method for direct amplification from crude nucleic acid samples
AU2003257177A8 (en)	2004-02-23	Improved compositions for in vitro amplification of nucleic acids
ATE414788T1 (de)	2008-12-15	Verfahren und kit zur tierartspezifischen dna- identifikation in einer probe
WO2004085681A3 (en)	2005-04-21	Global linear non-biased nucleic acid amplification
ATE520789T1 (de)	2011-09-15	Verfahren zur reduzierung des stotterns bei der mikrosatellitenamplifikation
ATE455864T1 (de)	2010-02-15	Verfahren zum nachweis von in einer polymerase- kettenreaktion amplifizierten nukleinsäuremolekülen
ATE455867T1 (de)	2010-02-15	Echtzeit-pcr unter zusatz von pyrophosphatase
DE60024679D1 (de)	2006-01-12	Methoden und zusammensetzungen zur detektion von spezies des mycobacterium avium-komplexes
ATE438849T1 (de)	2009-08-15	Verfahren zur detektion von staphylococcus epidermidis
ATE307906T1 (de)	2005-11-15	Verfahren zum nachweis von gram-positiven bakterien
CN120719009A (zh)	2025-09-30	一种双悬挂dsDNA结合CRISPR/Cas12a的miRNA传感器及其制备方法与应用