ATE329061T1 - Verfahren zur reduzierung unspezifischer amplifikation bei pcr - Google Patents

Verfahren zur reduzierung unspezifischer amplifikation bei pcr

Info

Publication number
ATE329061T1
ATE329061T1 AT02802916T AT02802916T ATE329061T1 AT E329061 T1 ATE329061 T1 AT E329061T1 AT 02802916 T AT02802916 T AT 02802916T AT 02802916 T AT02802916 T AT 02802916T AT E329061 T1 ATE329061 T1 AT E329061T1
Authority
AT
Austria
Prior art keywords
sorbitol
specific amplification
dmso
sample
reducing non
Prior art date
Application number
AT02802916T
Other languages
English (en)
Inventor
Ian J Mclaughlin
Sulekha Rao Coticone
Will Bloch
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Application granted granted Critical
Publication of ATE329061T1 publication Critical patent/ATE329061T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Amplifiers (AREA)
AT02802916T 2001-10-31 2002-07-30 Verfahren zur reduzierung unspezifischer amplifikation bei pcr ATE329061T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/998,887 US6783940B2 (en) 2001-10-31 2001-10-31 Method of reducing non-specific amplification in PCR

Publications (1)

Publication Number Publication Date
ATE329061T1 true ATE329061T1 (de) 2006-06-15

Family

ID=25545644

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02802916T ATE329061T1 (de) 2001-10-31 2002-07-30 Verfahren zur reduzierung unspezifischer amplifikation bei pcr

Country Status (8)

Country Link
US (1) US6783940B2 (de)
EP (1) EP1446508B1 (de)
JP (1) JP2005512572A (de)
AT (1) ATE329061T1 (de)
AU (1) AU2002365068A1 (de)
CA (1) CA2464885A1 (de)
DE (1) DE60212175T2 (de)
WO (1) WO2003054209A2 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080085515A1 (en) * 2000-03-24 2008-04-10 Eppendorf Array Technologies Sa (Eat) Identification of multiple biological (micro) organisms by detection of their nucleotide sequences on arrays
US20070298423A1 (en) * 2000-03-24 2007-12-27 Eppendorf Array Technologies Sa (Eat) Identification of multiple biological (micro) organisms by specific amplification and detection of their nucleotide sequences on arrays
US6841349B2 (en) * 2001-05-07 2005-01-11 Applera Corporation Applied Biosystems Group Methods for the reduction of stutter in microsatellite amplification
US7354742B2 (en) * 2002-02-22 2008-04-08 Ortho-Mcneil Pharmaceutical, Inc. Method for generating amplified RNA
EP1403385A1 (de) * 2002-09-27 2004-03-31 Institut National De La Sante Et De La Recherche Medicale (Inserm) Methode zur Darstellung der Replikation von HBV und zum Testen der Sensibilität von Wirkstoffen
WO2005038041A2 (en) * 2003-10-16 2005-04-28 Third Wave Technologies, Inc. Direct nucleic acid detection in bodily fluids
JP2007518425A (ja) * 2004-01-23 2007-07-12 バイオメリュー・インコーポレイテッド Ｈｃｖ３’非翻訳領域を効率的に増幅および検出するためのプライマーおよびプローブの設計
EP1725680B1 (de) * 2004-02-04 2009-12-23 QIAGEN North American Holdings, Inc. Zusammensetzungen auf dutp-basis zurverringerung der primeraggregatbildung während der nukleinsäureamplifikation
US8911942B2 (en) * 2004-05-20 2014-12-16 Quest Diagnostics Investments Incorporated Single label comparative hybridization
JP2008518621A (ja) * 2004-11-03 2008-06-05 サード・ウェーブ・テクノロジーズ・インク 一段階検出アッセイ法
JP2006262777A (ja) * 2005-03-24 2006-10-05 Japan Science & Technology Agency 夾雑物を含む試料を用いた核酸合成方法及び添加剤
WO2006119419A2 (en) * 2005-05-03 2006-11-09 Geunsook Jeon Materials and kits for use in hot-start pcr, and methods of amplifying nucleic acids in a polymerase chain reaction
US20070048757A1 (en) * 2005-05-31 2007-03-01 Applera Corporation Methods for characterizing cells using amplified micro rnas
JP5150829B2 (ja) * 2005-11-25 2013-02-27 株式会社ダナフォーム 核酸検出及び増幅方法
EP3167900B1 (de) 2006-03-29 2018-11-21 Merial Limited Impfstoff gegen streptokokken
US7772390B1 (en) 2006-07-18 2010-08-10 The Regents Of The University Of California Lipid mediated nucleic acid synthesis
KR100773561B1 (ko) * 2006-11-07 2007-11-05 삼성전자주식회사 다중 ｐｃｒ에서 비특이적 증폭을 감소시키는 장치 및 방법
US7507539B2 (en) * 2007-07-30 2009-03-24 Quest Diagnostics Investments Incorporated Substractive single label comparative hybridization
KR101419980B1 (ko) 2008-03-15 2014-07-15 홀로직, 인크. 증폭 반응 도중에 핵산 분자를 분석하기 위한 조성물 및 방법
EP2636757B1 (de) 2008-05-27 2016-11-23 Dako Denmark A/S Zusammensetzungen und Verfahren zum Nachweis von chromosomalen Abweichungen mit neuen Hybridisierungspuffern
EP2391733A1 (de) * 2009-01-27 2011-12-07 QIAGEN Gaithersburg, Inc. Thermophile-helikase-abhängige amplifikationstechnik mit homogenem fluoreszenzendpunktnachweis
US9303287B2 (en) 2009-02-26 2016-04-05 Dako Denmark A/S Compositions and methods for RNA hybridization applications
WO2013046033A1 (en) 2011-09-30 2013-04-04 Dako Denmark A/S Hybridization compositions and methods using formamide
US11118226B2 (en) 2011-10-21 2021-09-14 Agilent Technologies, Inc. Hybridization compositions and methods
CA2895324A1 (en) * 2012-12-18 2014-06-26 Merial, Inc. High resolution melt genotyping of ibv, csfv and ndv
US9630182B2 (en) * 2013-12-04 2017-04-25 Leidos Innovations Technology, Inc. Non-contact infrared thermocycling
US12065692B2 (en) 2017-08-03 2024-08-20 The Trustees Of Columbia University In The City Of New York Optical super-multiplexing by polyynes

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US5571674A (en) * 1989-09-18 1996-11-05 The Trustees Of Columbia University In The City Of New York DNA oligomers for use in detection of campylobacter pylori and methods of using such DNA oligomers
US5606045A (en) * 1990-05-15 1997-02-25 Diatron Corporation Nucleic acid probes and methods
GB9024005D0 (en) * 1990-11-05 1990-12-19 British Bio Technology Process for amplifying nucleic acid
US5364759B2 (en) 1991-01-31 1999-07-20 Baylor College Medicine Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats
DE4411588C1 (de) 1994-03-30 1995-09-28 Deutsches Rheuma Forschungszen Puffer für DNA- und RNA-Polymerase-Reaktionen
US5580728A (en) 1994-06-17 1996-12-03 Perlin; Mark W. Method and system for genotyping
US5545539A (en) 1994-10-18 1996-08-13 Genzyme Corporation Method for nucleotide sequence amplification
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6156512A (en) 1996-05-23 2000-12-05 Promega Corp Allelic ladders for short tandem repeat loci
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
JP4438110B2 (ja) * 1998-07-01 2010-03-24 東ソー株式会社 標的核酸の定量方法

Also Published As

Publication number Publication date
EP1446508B1 (de) 2006-06-07
EP1446508A2 (de) 2004-08-18
DE60212175T2 (de) 2006-10-12
WO2003054209A3 (en) 2003-12-04
AU2002365068A1 (en) 2003-07-09
US20030104395A1 (en) 2003-06-05
EP1446508A4 (de) 2005-03-09
WO2003054209A2 (en) 2003-07-03
US6783940B2 (en) 2004-08-31
CA2464885A1 (en) 2003-07-03
DE60212175D1 (de) 2006-07-20
JP2005512572A (ja) 2005-05-12

Similar Documents

Publication Publication Date Title
DE60212175D1 (de) Verfahren zur reduzierung unspezifischer amplifikation bei pcr
DE60220025D1 (de) Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
WO2002072773A3 (en) Methods and compositions for amplification of rna sequences
DE60143960D1 (de) Äuren
US8623602B2 (en) Lysis and reverse transcription for MRNA quantification
WO2005098042A3 (en) Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity
US20150050653A1 (en) Sample Preparation for in Situ Nucleic Acid Analysis, Methods and Compositions Therefor
ES2169115T3 (es) Procedimiento para la cuantificacion de muestras de adn genomico.
WO2006076017A3 (en) Methods and kits for identifying target nucleotides in mixed populations
DE60143314D1 (de) Estimmung der präsenz von cryptosporidium organismen in einer testprobe
US8153401B2 (en) Method for direct amplification from crude nucleic acid samples
WO2002064842A3 (en) Quantitative epstein barr virus pcr rapid assay
WO2004050911A3 (en) Method for lowering both sequence variations and increase of base lines effects in diagnostic hybridisation assay, assay for performing such a method and probe for use in the assay
Zwirglmaier et al. Rapid detection of SARS-CoV-2 by pulse-controlled amplification (PCA)
US20080233587A1 (en) Method for direct amplification from crude nucleic acid samples
ATE526418T1 (de) Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren
DE602004030036D1 (de) Multiplexanalyse von Nukleinsäuren zur Bestimmung von Genotypen des humanen Papillomavirus
WO2004085681A3 (en) Global linear non-biased nucleic acid amplification
ATE520789T1 (de) Verfahren zur reduzierung des stotterns bei der mikrosatellitenamplifikation
ATE455864T1 (de) Verfahren zum nachweis von in einer polymerase- kettenreaktion amplifizierten nukleinsäuremolekülen
ATE455867T1 (de) Echtzeit-pcr unter zusatz von pyrophosphatase
DE60024679D1 (de) Methoden und zusammensetzungen zur detektion von spezies des mycobacterium avium-komplexes
ATE438849T1 (de) Verfahren zur detektion von staphylococcus epidermidis
AU2002312804A1 (en) Method for detecting gram-positive bacteria having a high gc content
CN120719009A (zh) 一种双悬挂dsDNA结合CRISPR/Cas12a的miRNA传感器及其制备方法与应用

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties