ATE455867T1 - Echtzeit-pcr unter zusatz von pyrophosphatase - Google Patents
Echtzeit-pcr unter zusatz von pyrophosphataseInfo
- Publication number
- ATE455867T1 ATE455867T1 AT05019405T AT05019405T ATE455867T1 AT E455867 T1 ATE455867 T1 AT E455867T1 AT 05019405 T AT05019405 T AT 05019405T AT 05019405 T AT05019405 T AT 05019405T AT E455867 T1 ATE455867 T1 AT E455867T1
- Authority
- AT
- Austria
- Prior art keywords
- pyrophosphatase
- real
- addition
- time pcr
- nucleic acid
- Prior art date
Links
- 102000009609 Pyrophosphatases Human genes 0.000 title abstract 2
- 108010009413 Pyrophosphatases Proteins 0.000 title abstract 2
- 238000003753 real-time PCR Methods 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP04021399 | 2004-09-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE455867T1 true ATE455867T1 (de) | 2010-02-15 |
Family
ID=34926467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05019405T ATE455867T1 (de) | 2004-09-09 | 2005-09-07 | Echtzeit-pcr unter zusatz von pyrophosphatase |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20060051796A1 (de) |
| JP (1) | JP2006075167A (de) |
| CN (1) | CN100478453C (de) |
| AT (1) | ATE455867T1 (de) |
| CA (1) | CA2515242C (de) |
| DE (1) | DE602005019005D1 (de) |
| ES (1) | ES2338662T3 (de) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008080254A1 (en) * | 2006-12-29 | 2008-07-10 | Honeywell International Inc. | Reaction buffer for microarray |
| CA2719718C (en) * | 2008-03-28 | 2019-07-02 | Mdna Life Sciences Inc. | Aberrant mitochondrial dna, associated fusion transcripts and hybridization probes therefor |
| US8346485B2 (en) | 2008-11-25 | 2013-01-01 | Quest Diagnostics Investments Incorporated | Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction |
| CN102482720B (zh) | 2009-09-10 | 2014-10-08 | 3M创新有限公司 | 对多重核酸扩增中的光谱串扰的补偿 |
| KR20240171383A (ko) * | 2023-05-30 | 2024-12-09 | 홍익대학교 산학협력단 | 전기화학적 방식을 이용한 중합효소 연쇄 반응의 피로인산 이온 농도 변화 측정 방법 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5618711A (en) * | 1986-08-22 | 1997-04-08 | Hoffmann-La Roche Inc. | Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase |
| US5498523A (en) * | 1988-07-12 | 1996-03-12 | President And Fellows Of Harvard College | DNA sequencing with pyrophosphatase |
| US5118801A (en) * | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| JP4540754B2 (ja) * | 1996-06-04 | 2010-09-08 | ユニバーシティ オブ ユタ リサーチ ファウンデーション | Ｐｃｒ中のハイブリダイゼーションのモニタリング |
| DE19653494A1 (de) * | 1996-12-20 | 1998-06-25 | Svante Dr Paeaebo | Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung |
| EP1272668B1 (de) * | 2000-04-03 | 2007-02-14 | Corixa Corporation | Verfahren, zusammensetzungen und kits zur identifizierung und überwachung von brustkrebs |
| GB0110501D0 (en) * | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
| US20040170981A1 (en) * | 2003-02-10 | 2004-09-02 | Mckenney Keith | Real-time polymerase chain reaction using large target amplicons |
-
2005
- 2005-08-25 US US11/211,481 patent/US20060051796A1/en not_active Abandoned
- 2005-09-06 CA CA2515242A patent/CA2515242C/en not_active Expired - Fee Related
- 2005-09-07 DE DE602005019005T patent/DE602005019005D1/de not_active Expired - Lifetime
- 2005-09-07 AT AT05019405T patent/ATE455867T1/de not_active IP Right Cessation
- 2005-09-07 ES ES05019405T patent/ES2338662T3/es not_active Expired - Lifetime
- 2005-09-08 CN CNB2005100999115A patent/CN100478453C/zh not_active Expired - Fee Related
- 2005-09-08 JP JP2005261187A patent/JP2006075167A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN100478453C (zh) | 2009-04-15 |
| CA2515242A1 (en) | 2006-03-09 |
| JP2006075167A (ja) | 2006-03-23 |
| CA2515242C (en) | 2010-11-02 |
| DE602005019005D1 (de) | 2010-03-11 |
| ES2338662T3 (es) | 2010-05-11 |
| US20060051796A1 (en) | 2006-03-09 |
| CN1746314A (zh) | 2006-03-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |