ATE332371T1 - Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese - Google Patents

Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese

Info

Publication number
ATE332371T1
ATE332371T1 AT00943373T AT00943373T ATE332371T1 AT E332371 T1 ATE332371 T1 AT E332371T1 AT 00943373 T AT00943373 T AT 00943373T AT 00943373 T AT00943373 T AT 00943373T AT E332371 T1 ATE332371 T1 AT E332371T1
Authority
AT
Austria
Prior art keywords
nucleic acid
synthesis
relates
inhibitors
compositions
Prior art date
Application number
AT00943373T
Other languages
English (en)
Inventor
Mekbib Astatke
Deb Chatterjee
Gary Gerard
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ATE332371T1 publication Critical patent/ATE332371T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plant Pathology (AREA)
  • Communicable Diseases (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Oncology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • AIDS & HIV (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT00943373T 1999-07-02 2000-06-30 Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese ATE332371T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US14207299P 1999-07-02 1999-07-02

Publications (1)

Publication Number Publication Date
ATE332371T1 true ATE332371T1 (de) 2006-07-15

Family

ID=22498453

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00943373T ATE332371T1 (de) 1999-07-02 2000-06-30 Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese

Country Status (8)

Country Link
EP (1) EP1196559B1 (de)
JP (2) JP2003504018A (de)
AT (1) ATE332371T1 (de)
AU (1) AU782555B2 (de)
CA (1) CA2377780A1 (de)
DE (1) DE60029225T2 (de)
NZ (1) NZ516894A (de)
WO (1) WO2001002559A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100351393C (zh) 2001-02-15 2007-11-28 宝生物工程株式会社 核苷酸多态性的检测方法
AU2003267785C1 (en) * 2002-09-13 2009-12-24 Replicor, Inc. Non-sequence complementary antiviral oligonucleotides
BRPI0516619B1 (pt) * 2004-10-18 2016-02-10 Univ Brandeis reagentes, kit de reagentes e conjunto de oligonucleotídeos para realização de uma amplificação por reação em cadeia de polimerase
WO2007038422A2 (en) 2005-09-26 2007-04-05 Allelogic Biosciences Corp. Oligonucleotides as temperature-sensitive inhibitors for dna polymerases
EP2458011B1 (de) * 2005-10-03 2013-07-24 Life Technologies Corporation Zusammensetzungen, Verfahren und Kits zur Amplifikation von Nukleinsäuren
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
CN103228785B (zh) 2010-11-24 2016-09-07 株式会社钟化 扩增核酸的检测方法和检测装置
WO2013154898A1 (en) * 2012-04-12 2013-10-17 Ong, Jennifer Synthetic nucleic acids for polymerization reactions
CN104254620B (zh) * 2012-04-27 2022-11-08 株式会社钟化 核酸的扩增方法及扩增核酸的检测方法
JP6691380B2 (ja) * 2013-11-22 2020-04-28 株式会社カネカ 短鎖ｒｎａの検出方法
CN111771001B (zh) * 2018-01-05 2024-09-06 F·霍夫曼-罗氏股份公司 通过芳香族化合物增强核酸聚合
KR102275038B1 (ko) * 2019-12-11 2021-07-08 (주) 제노텍 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트
CN115135664A (zh) * 2020-06-22 2022-09-30 苏州新海生物科技股份有限公司 一种核酸配体及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763173A (en) * 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
JP4335975B2 (ja) * 1995-06-07 2009-09-30 ギリード・サイエンシズ・インコーポレーテッド Ｄｎａポリメラーゼに対する結合・阻害核酸リガンド
US5760012A (en) * 1996-05-01 1998-06-02 Thomas Jefferson University Methods and compounds for curing diseases caused by mutations

Also Published As

Publication number Publication date
CA2377780A1 (en) 2001-01-11
AU782555B2 (en) 2005-08-11
NZ516894A (en) 2004-06-25
JP2003504018A (ja) 2003-02-04
EP1196559A1 (de) 2002-04-17
JP2011041572A (ja) 2011-03-03
AU5785000A (en) 2001-01-22
EP1196559B1 (de) 2006-07-05
DE60029225T2 (de) 2007-07-05
WO2001002559A9 (en) 2002-07-25
WO2001002559A1 (en) 2001-01-11
DE60029225D1 (de) 2006-08-17

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