ATE339686T1 - Stabilisierung von hochsensitiven nukleinsäurefärbungen in wässriger lösung - Google Patents
Stabilisierung von hochsensitiven nukleinsäurefärbungen in wässriger lösungInfo
- Publication number
- ATE339686T1 ATE339686T1 AT01918895T AT01918895T ATE339686T1 AT E339686 T1 ATE339686 T1 AT E339686T1 AT 01918895 T AT01918895 T AT 01918895T AT 01918895 T AT01918895 T AT 01918895T AT E339686 T1 ATE339686 T1 AT E339686T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- stabilization
- aqueous solution
- highly sensitive
- acid staining
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 239000007864 aqueous solution Substances 0.000 title 1
- 230000006641 stabilisation Effects 0.000 title 1
- 238000011105 stabilization Methods 0.000 title 1
- 238000010186 staining Methods 0.000 title 1
- 239000003125 aqueous solvent Substances 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 239000000499 gel Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
- 239000003381 stabilizer Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Saccharide Compounds (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/535,129 US6365341B1 (en) | 2000-03-24 | 2000-03-24 | Stabilization of highly sensitive nucleic acid stains in aqueous solutions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE339686T1 true ATE339686T1 (de) | 2006-10-15 |
Family
ID=24132962
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01918895T ATE339686T1 (de) | 2000-03-24 | 2001-03-22 | Stabilisierung von hochsensitiven nukleinsäurefärbungen in wässriger lösung |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US6365341B1 (de) |
| EP (1) | EP1272655B1 (de) |
| AT (1) | ATE339686T1 (de) |
| DE (1) | DE60123030T2 (de) |
| DK (1) | DK1272655T3 (de) |
| ES (1) | ES2272453T3 (de) |
| WO (1) | WO2001073110A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7776529B2 (en) | 2003-12-05 | 2010-08-17 | Life Technologies Corporation | Methine-substituted cyanine dye compounds |
| EP2607431B1 (de) * | 2003-12-05 | 2016-08-24 | Life Technologies Corporation | Cyanin Farbstoffverbindungen |
| JP5306811B2 (ja) * | 2005-05-11 | 2013-10-02 | ライフ テクノロジーズ コーポレーション | 2本鎖dnaへの高い選択性を有する蛍光化学物質及びそれらの使用 |
| US20100041045A1 (en) * | 2007-11-15 | 2010-02-18 | Sigma-Aldrich Co. | Nucleic acid fluorescent stains |
| US20090131279A1 (en) * | 2007-11-15 | 2009-05-21 | Sigma Aldrich Company | Nucleic acid fluorescent stains |
| CN104099419B (zh) * | 2014-07-17 | 2017-09-08 | 广州华峰生物科技有限公司 | 一种核酸显色剂及其应用 |
| US20180355345A1 (en) * | 2015-07-30 | 2018-12-13 | Arcis Biotechnology Holdings Limited | Method and composition |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5658751A (en) | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5436134A (en) | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
-
2000
- 2000-03-24 US US09/535,129 patent/US6365341B1/en not_active Expired - Lifetime
-
2001
- 2001-03-22 EP EP01918895A patent/EP1272655B1/de not_active Expired - Lifetime
- 2001-03-22 WO PCT/US2001/009080 patent/WO2001073110A1/en not_active Ceased
- 2001-03-22 ES ES01918895T patent/ES2272453T3/es not_active Expired - Lifetime
- 2001-03-22 DK DK01918895T patent/DK1272655T3/da active
- 2001-03-22 DE DE60123030T patent/DE60123030T2/de not_active Expired - Lifetime
- 2001-03-22 AT AT01918895T patent/ATE339686T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP1272655B1 (de) | 2006-09-13 |
| ES2272453T3 (es) | 2007-05-01 |
| WO2001073110A1 (en) | 2001-10-04 |
| DK1272655T3 (da) | 2006-12-18 |
| DE60123030T2 (de) | 2007-03-29 |
| EP1272655A4 (de) | 2004-04-07 |
| US6365341B1 (en) | 2002-04-02 |
| DE60123030D1 (de) | 2006-10-26 |
| EP1272655A1 (de) | 2003-01-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |