ATE340873T1 - Verfahren zur durchführung einer subtraktiven hybridisierung - Google Patents

Verfahren zur durchführung einer subtraktiven hybridisierung

Info

Publication number: ATE340873T1
Authority: AT; Austria
Prior art keywords: subtractive hybridization; performing subtractive; relates; oligonucleotides; reagents
Prior art date: 2000-08-02

Application number

AT01957439T

Other languages

English (en)

Inventor

Larry G Birkenmeyer

Thomas P Leary

A Scott Muerhoff

Suresh M Desai

Isa K Mushahwar

Original Assignee

Abbott Lab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-08-02

Filing date

2001-08-02

Publication date

2006-10-15

2001-08-02 Application filed by Abbott Lab filed Critical Abbott Lab

2006-10-15 Application granted granted Critical

2006-10-15 Publication of ATE340873T1 publication Critical patent/ATE340873T1/de

Links

238000000034 method Methods 0.000 title abstract 3
238000009396 hybridization Methods 0.000 title abstract 2
108091034117 Oligonucleotide Proteins 0.000 abstract 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
239000003153 chemical reaction reagent Substances 0.000 abstract 1
238000002955 isolation Methods 0.000 abstract 1
238000012986 modification Methods 0.000 abstract 1
230000004048 modification Effects 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Analytical Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Biotechnology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
Microbiology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Led Device Packages (AREA)
Saccharide Compounds (AREA)

AT01957439T 2000-08-02 2001-08-02 Verfahren zur durchführung einer subtraktiven hybridisierung ATE340873T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US09/631,349 US6455255B1 (en)	2000-08-02	2000-08-02	Method of performing subtractive hybridization using RDA

Publications (1)

Publication Number	Publication Date
ATE340873T1 true ATE340873T1 (de)	2006-10-15

Family

ID=24530821

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT01957439T ATE340873T1 (de)	2000-08-02	2001-08-02	Verfahren zur durchführung einer subtraktiven hybridisierung

Country Status (8)

Country	Link
US (1)	US6455255B1 (de)
EP (1)	EP1364048B1 (de)
JP (1)	JP4856842B2 (de)
AT (1)	ATE340873T1 (de)
CA (1)	CA2417501A1 (de)
DE (1)	DE60123481T2 (de)
ES (1)	ES2272516T3 (de)
WO (1)	WO2002010458A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN1264744A (zh) *	1999-02-26	2000-08-30	上海生元基因开发有限公司	一种通过循环差减进行大规模cDNA克隆和测序的方法
KR100870222B1 (ko) *	2000-12-19	2008-11-24	산또리 가부시키가이샤	유전자 농축 방법
US6632611B2 (en) *	2001-07-20	2003-10-14	Affymetrix, Inc.	Method of target enrichment and amplification
GB2412170A (en) *	2004-03-16	2005-09-21	Plant Bioscience Ltd	A method for enriching for target nucleic acids in a sample
EP1854882A4 (de) *	2005-03-01	2010-09-22	Wako Pure Chem Ind Ltd	Verfahren zur gewinnung eines subtraktionspolynukleotids
WO2007100384A2 (en) *	2005-10-24	2007-09-07	Invitrogen Corporation	Three frame cdna expression libraries for functional screening of proteins
US9388457B2 (en)	2007-09-14	2016-07-12	Affymetrix, Inc.	Locus specific amplification using array probes
WO2011030154A1 (en) *	2009-09-12	2011-03-17	Zoragen Biotechnologies Llp	Detection of quantitative genetic differences

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4683195A (en)	1986-01-30	1987-07-28	Cetus Corporation	Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5436142A (en) *	1992-11-12	1995-07-25	Cold Spring Harbor Laboratory	Methods for producing probes capable of distingushing variant genomic sequences
US5958738A (en)	1997-03-24	1999-09-28	Roche Diagnostics Corporation	Procedure for subtractive hybridization and difference analysis
EP1032705B1 (de) *	1997-10-30	2011-12-14	Cold Spring Harbor Laboratory	Sondenanordnungen und deren verwendungen für verfahren zur dns-unterscheidung
ATE333499T1 (de) *	1998-03-18	2006-08-15	Quark Biotech Inc	Selektion/subtraktionsansatz zur genidentifizierung
EP1127163A2 (de) *	1998-11-02	2001-08-29	The Brigham & Women's Hospital, Inc.	Eine methode zur genomischen subtraktiven hybridisierung
JP2000139461A (ja) *	1998-11-06	2000-05-23	Hitachi Koki Co Ltd	核酸サブトラクション法及びその装置
CN1264744A (zh) *	1999-02-26	2000-08-30	上海生元基因开发有限公司	一种通过循环差减进行大规模cDNA克隆和测序的方法
JP2000308489A (ja) *	1999-04-28	2000-11-07	Dai Ichi Seiyaku Co Ltd	微小変異ｄｎａ検出方法

2000
- 2000-08-02 US US09/631,349 patent/US6455255B1/en not_active Expired - Fee Related
2001
- 2001-08-02 ES ES01957439T patent/ES2272516T3/es not_active Expired - Lifetime
- 2001-08-02 EP EP01957439A patent/EP1364048B1/de not_active Expired - Lifetime
- 2001-08-02 DE DE60123481T patent/DE60123481T2/de not_active Expired - Lifetime
- 2001-08-02 JP JP2002516374A patent/JP4856842B2/ja not_active Expired - Fee Related
- 2001-08-02 WO PCT/US2001/024480 patent/WO2002010458A2/en not_active Ceased
- 2001-08-02 CA CA002417501A patent/CA2417501A1/en not_active Abandoned
- 2001-08-02 AT AT01957439T patent/ATE340873T1/de not_active IP Right Cessation

Also Published As

Publication number	Publication date
DE60123481D1 (de)	2006-11-09
WO2002010458A3 (en)	2003-09-04
JP2004516014A (ja)	2004-06-03
JP4856842B2 (ja)	2012-01-18
DE60123481T2 (de)	2007-06-21
WO2002010458A8 (en)	2007-07-05
US6455255B1 (en)	2002-09-24
CA2417501A1 (en)	2002-02-07
ES2272516T3 (es)	2007-05-01
EP1364048B1 (de)	2006-09-27
WO2002010458A2 (en)	2002-02-07
EP1364048A2 (de)	2003-11-26

Legal Events

Date	Code	Title	Description
2007-03-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

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