ATE341645T1 - Verfahren für die reverse transkription - Google Patents
Verfahren für die reverse transkriptionInfo
- Publication number
- ATE341645T1 ATE341645T1 AT98918516T AT98918516T ATE341645T1 AT E341645 T1 ATE341645 T1 AT E341645T1 AT 98918516 T AT98918516 T AT 98918516T AT 98918516 T AT98918516 T AT 98918516T AT E341645 T1 ATE341645 T1 AT E341645T1
- Authority
- AT
- Austria
- Prior art keywords
- reverse transcription
- dependent
- synthesis
- improving
- full
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 238000010839 reverse transcription Methods 0.000 title 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 102100034343 Integrase Human genes 0.000 abstract 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 230000001419 dependent effect Effects 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Photovoltaic Devices (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Gyroscopes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/840,474 US6030814A (en) | 1997-04-21 | 1997-04-21 | Reverse transcription method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE341645T1 true ATE341645T1 (de) | 2006-10-15 |
Family
ID=25282475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT98918516T ATE341645T1 (de) | 1997-04-21 | 1998-04-15 | Verfahren für die reverse transkription |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6030814A (de) |
| EP (1) | EP0977891B1 (de) |
| JP (1) | JP2000513585A (de) |
| CN (1) | CN1261406A (de) |
| AT (1) | ATE341645T1 (de) |
| AU (1) | AU743907B2 (de) |
| CA (1) | CA2291855C (de) |
| DE (1) | DE69836076T2 (de) |
| WO (1) | WO1998048053A1 (de) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19920611A1 (de) * | 1999-05-05 | 2000-11-09 | Roche Diagnostics Gmbh | Verfahren zur 5'-Cap-abhängigen Anreicherung von cDNAs |
| US6436677B1 (en) * | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
| US6835537B1 (en) | 2000-09-29 | 2004-12-28 | Myriad Genetics, Inc. | Method for equalizing band intensities on sequencing gels |
| US20070020622A1 (en) * | 2001-09-14 | 2007-01-25 | Invitrogen Corporation | DNA Polymerases and mutants thereof |
| WO2004048594A2 (en) | 2002-11-21 | 2004-06-10 | Epicentre Technologies | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
| EP1604040B1 (de) * | 2003-03-07 | 2010-10-13 | Rubicon Genomics, Inc. | Amplifikation und analyse von gesamtgenom- und gesamttranskriptom- bibliotheken, die durch ein dns-polymerisierungsverfahren produziert wurden |
| US8206913B1 (en) | 2003-03-07 | 2012-06-26 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process |
| TW200506052A (en) * | 2003-05-07 | 2005-02-16 | Takara Bio Inc | Method of analyzing DNA region |
| AU2006287548A1 (en) * | 2005-09-06 | 2007-03-15 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
| JP5479895B2 (ja) * | 2006-07-25 | 2014-04-23 | アジレント・テクノロジーズ・インク | Dnaポリメラーゼの保存及び使用のための両性イオン洗剤 |
| US8039214B2 (en) | 2007-06-29 | 2011-10-18 | Cellscript, Inc. | Synthesis of tagged nucleic acids |
| MX2013012709A (es) | 2011-05-02 | 2013-12-06 | Pioneer Hi Bred Int | Estrategia de analisis de arnm bacteriano para descubrir nuevas moleculas de acido nucleico codificante de pesticidas. |
| CN113234802A (zh) * | 2021-06-04 | 2021-08-10 | 翌圣生物科技(上海)股份有限公司 | 泊洛沙姆在提高逆转录酶效率或提高逆转录酶抑制剂耐受性中的应用及添加剂混合物 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5322770A (en) * | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
| US5693517A (en) * | 1987-06-17 | 1997-12-02 | Roche Molecular Systems, Inc. | Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions |
| US5310652A (en) * | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
| WO1991009944A2 (en) * | 1989-12-22 | 1991-07-11 | F.Hoffmann-La Roche Ag | High temperature reverse transcriptases |
| US5407800A (en) * | 1986-08-22 | 1995-04-18 | Hoffmann-La Roche Inc. | Reverse transcription with Thermus thermophilus polymerase |
| DE4411588C1 (de) * | 1994-03-30 | 1995-09-28 | Deutsches Rheuma Forschungszen | Puffer für DNA- und RNA-Polymerase-Reaktionen |
| US5545539A (en) * | 1994-10-18 | 1996-08-13 | Genzyme Corporation | Method for nucleotide sequence amplification |
-
1997
- 1997-04-21 US US08/840,474 patent/US6030814A/en not_active Expired - Lifetime
-
1998
- 1998-04-15 AT AT98918516T patent/ATE341645T1/de not_active IP Right Cessation
- 1998-04-15 WO PCT/US1998/007997 patent/WO1998048053A1/en not_active Ceased
- 1998-04-15 CN CN98806390A patent/CN1261406A/zh active Pending
- 1998-04-15 CA CA002291855A patent/CA2291855C/en not_active Expired - Lifetime
- 1998-04-15 EP EP98918516A patent/EP0977891B1/de not_active Expired - Lifetime
- 1998-04-15 JP JP10546266A patent/JP2000513585A/ja active Pending
- 1998-04-15 AU AU71423/98A patent/AU743907B2/en not_active Expired
- 1998-04-15 DE DE69836076T patent/DE69836076T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US6030814A (en) | 2000-02-29 |
| DE69836076D1 (de) | 2006-11-16 |
| JP2000513585A (ja) | 2000-10-17 |
| CA2291855C (en) | 2005-01-11 |
| EP0977891A1 (de) | 2000-02-09 |
| CA2291855A1 (en) | 1998-10-29 |
| CN1261406A (zh) | 2000-07-26 |
| DE69836076T2 (de) | 2007-04-19 |
| AU743907B2 (en) | 2002-02-07 |
| WO1998048053A1 (en) | 1998-10-29 |
| AU7142398A (en) | 1998-11-13 |
| EP0977891B1 (de) | 2006-10-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE341645T1 (de) | Verfahren für die reverse transkription | |
| DE50304177D1 (de) | Verfahren zur Herstellung eines Bohrers oder Fräsers | |
| DE68913215D1 (de) | Verfahren zur Herstellung hydraulischer Dämpfungsspalten. | |
| AU1408900A (en) | Method for synthesizing cdna | |
| DE50303174D1 (de) | Verfahren und Vorrichtung zur Herstellung von fliessfähigem Metallschaum | |
| DE68911215D1 (de) | Verfahren zur Herstellung von hochantikorrosiven, oberflächenbehandelten Stahlplatten. | |
| DE69910113D1 (de) | Verfahren zur Herstellung von Stickstofftrifluorid durch Gas-Feststoffreaktion | |
| DE502005009449D1 (de) | Metallreflektor und Verfahren zu dessen Herstellung | |
| ATE425121T1 (de) | Verfahren zur umwandlung von polysulfanen | |
| DE502004001985D1 (de) | Verfahren zur Herstellung von expandiertem Graphit | |
| MXPA02011762A (es) | Proceso para la preparacion de compuestos metales de transicion y su empleo para la polimerizacion de olefinas. | |
| EA199900772A1 (ru) | Способ селективного эпоксидирования для получения лекарственных соединений | |
| ATE417131T1 (de) | Verfahren zur herstellung von metallschaumkörpern auf basis von zink | |
| ATE359252T1 (de) | Verfahren zur herstellung von olefinen | |
| ATE244706T1 (de) | Enantiomere von mercaptolactonen und verfahren zu ihrer herstellung | |
| ATE352837T1 (de) | Verfahren zur steuerung der dauer bei der sprachsynthese | |
| DE60011463D1 (de) | Verfahren zur herstellung von 24(s)-hydroxyvitamin d2 | |
| ATE316141T1 (de) | Für die gdp-fucose-pyrophosphorylase kodierende nukleinsäure | |
| ATE176280T1 (de) | Enzymatisches verfahren zur herstellung von morphin-6-glucuroniden oder substituierten morphin-6-glucuroniden | |
| ATE347609T1 (de) | Enzymatisches verfahren zur herstellung von organofluorverbindungen | |
| DE60014330D1 (de) | Verfahren zur herstellung von 1,4,7,10-tetraazacyclododecan | |
| MXPA04004526A (es) | N-metil-homocisteinas, su uso y metodo para su produccion. | |
| ID24516A (id) | Proses penyalutan elastomer-elastomer silikon | |
| NO955128L (no) | Fremgangsmåte til fremstilling av et ekspansjonsanker av et korrosjonsbestandig stål | |
| ATE358660T1 (de) | Verfahren zur hydrierung von aceton |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |