ATE342378T1 - Verfahren das auf den gekoppelten reactionen der polymerase-kettenreaktion (pcr)-verdauung mit restriktionsenzymen und ligasenachweis basiert - Google Patents

Verfahren das auf den gekoppelten reactionen der polymerase-kettenreaktion (pcr)-verdauung mit restriktionsenzymen und ligasenachweis basiert

Info

Publication number
ATE342378T1
ATE342378T1 AT00918081T AT00918081T ATE342378T1 AT E342378 T1 ATE342378 T1 AT E342378T1 AT 00918081 T AT00918081 T AT 00918081T AT 00918081 T AT00918081 T AT 00918081T AT E342378 T1 ATE342378 T1 AT E342378T1
Authority
AT
Austria
Prior art keywords
abundance
digestion
polymerase chain
chain reaction
ligase detection
Prior art date
Application number
AT00918081T
Other languages
English (en)
Inventor
Francis Barany
Joseph P Day
Robert P Hammer
Donald E Bergstrom
Original Assignee
Cornell Res Foundation Inc
Univ Louisiana State
Purdue Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Res Foundation Inc, Univ Louisiana State, Purdue Research Foundation filed Critical Cornell Res Foundation Inc
Application granted granted Critical
Publication of ATE342378T1 publication Critical patent/ATE342378T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT00918081T 1999-03-19 2000-03-17 Verfahren das auf den gekoppelten reactionen der polymerase-kettenreaktion (pcr)-verdauung mit restriktionsenzymen und ligasenachweis basiert ATE342378T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US12525199P 1999-03-19 1999-03-19

Publications (1)

Publication Number Publication Date
ATE342378T1 true ATE342378T1 (de) 2006-11-15

Family

ID=22418843

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00918081T ATE342378T1 (de) 1999-03-19 2000-03-17 Verfahren das auf den gekoppelten reactionen der polymerase-kettenreaktion (pcr)-verdauung mit restriktionsenzymen und ligasenachweis basiert

Country Status (7)

Country Link
EP (1) EP1165838B1 (de)
JP (1) JP4601830B2 (de)
AT (1) ATE342378T1 (de)
AU (1) AU771615B2 (de)
CA (1) CA2369533A1 (de)
DE (1) DE60031253D1 (de)
WO (1) WO2000056929A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7807447B1 (en) 2000-08-25 2010-10-05 Merck Sharp & Dohme Corp. Compositions and methods for exon profiling
US6713257B2 (en) 2000-08-25 2004-03-30 Rosetta Inpharmatics Llc Gene discovery using microarrays
CA2555704A1 (en) 2004-02-10 2005-08-25 Cornell Research Foundation, Inc. Method for detection of promoter methylation status
CN105189746B (zh) 2013-03-14 2018-12-04 宝生物工程株式会社 用于使用耐热的错配内切核酸酶的方法
CN107002067A (zh) 2014-09-11 2017-08-01 宝生物工程株式会社 利用热稳定性错配内切核酸酶的方法
WO2020227127A2 (en) * 2019-05-03 2020-11-12 Cornell University Method and markers for identifying and quantifying of nucleic acid sequence, mutation, copy number, or methylation changes using combinations of nuclease, ligation, dna repair, and polymerase reactions with carryover prevention
WO2020227100A1 (en) * 2019-05-03 2020-11-12 Cornell University Markers for identifying and quantifying of nucleic acid sequence mutation, expression, splice variant, translocation, copy number, or methylation changes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0607151B1 (de) * 1992-06-17 2002-11-13 City Of Hope Verfahren zum nachweis und unterscheiden von nukleinsäuresequenzen
GB9422814D0 (en) * 1994-11-11 1995-01-04 Medinnova Sf Chemical method
EP1736554B1 (de) * 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Bestimmung von Unterschieden in der Nukleinsäuresequenz mittels einer Kombination von Ligasebestimmung und Polymerasekettenreaktion
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction

Also Published As

Publication number Publication date
EP1165838B1 (de) 2006-10-11
WO2000056929A3 (en) 2001-08-09
DE60031253D1 (de) 2006-11-23
JP4601830B2 (ja) 2010-12-22
CA2369533A1 (en) 2000-09-28
WO2000056929A2 (en) 2000-09-28
AU771615B2 (en) 2004-04-01
EP1165838A2 (de) 2002-01-02
AU3895500A (en) 2000-10-09
JP2003510011A (ja) 2003-03-18

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