ATE342990T1 - Oberflächenexpressionsvektoren mit pgsbca, dem für poly-gamma-glutamat-synthetase codierenden gen, sowie verfahren zur expression eines zielproteins an der oberfläche eines den vektor benutzenden mikroorganismus - Google Patents

Oberflächenexpressionsvektoren mit pgsbca, dem für poly-gamma-glutamat-synthetase codierenden gen, sowie verfahren zur expression eines zielproteins an der oberfläche eines den vektor benutzenden mikroorganismus

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Publication number
ATE342990T1
ATE342990T1 AT02755966T AT02755966T ATE342990T1 AT E342990 T1 ATE342990 T1 AT E342990T1 AT 02755966 T AT02755966 T AT 02755966T AT 02755966 T AT02755966 T AT 02755966T AT E342990 T1 ATE342990 T1 AT E342990T1
Authority
AT
Austria
Prior art keywords
vector
pgsbca
gamma
target protein
poly
Prior art date
Application number
AT02755966T
Other languages
English (en)
Inventor
Moon-Hee Sung
Seung-Pyo Hong
Jong-Su Lee
Chang-Min Jung
Chul-Joong Kim
Kenji Soda
Makoto Ashiuchi
Original Assignee
Bioleaders Corp
M D Lab Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioleaders Corp, M D Lab Co Ltd filed Critical Bioleaders Corp
Application granted granted Critical
Publication of ATE342990T1 publication Critical patent/ATE342990T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288—Transferases for other substituted phosphate groups (2.7.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01133—Xylosylprotein 4-beta-galactosyltransferase (2.4.1.133)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/08—Transferases for other substituted phosphate groups (2.7.8)
    • C12Y207/08005—CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase (2.7.8.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT02755966T 2001-08-10 2002-08-09 Oberflächenexpressionsvektoren mit pgsbca, dem für poly-gamma-glutamat-synthetase codierenden gen, sowie verfahren zur expression eines zielproteins an der oberfläche eines den vektor benutzenden mikroorganismus ATE342990T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR20010048373 2001-08-10

Publications (1)

Publication Number Publication Date
ATE342990T1 true ATE342990T1 (de) 2006-11-15

Family

ID=19713096

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02755966T ATE342990T1 (de) 2001-08-10 2002-08-09 Oberflächenexpressionsvektoren mit pgsbca, dem für poly-gamma-glutamat-synthetase codierenden gen, sowie verfahren zur expression eines zielproteins an der oberfläche eines den vektor benutzenden mikroorganismus

Country Status (8)

Country Link
US (1) US7553636B2 (de)
EP (1) EP1421195B1 (de)
JP (1) JP4458844B2 (de)
KR (1) KR100469800B1 (de)
CN (1) CN1311082C (de)
AT (1) ATE342990T1 (de)
DE (1) DE60215521T2 (de)
WO (1) WO2003014360A1 (de)

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KR100525759B1 (ko) * 2002-10-17 2005-11-03 한국생명공학연구원 바실러스 속 균주 유래의 폴리 감마 글루탐산합성유전자를 이용한 펩타이드 항생물질 Ｐ5와Ａｎａｌ3의 표면 발현 방법 및 그의 용도
WO2004035795A1 (en) 2002-10-17 2004-04-29 Bioleaders Corporation Vector for anti-hpv vaccine and transformed microorganism by the vector
EP1629104A4 (de) * 2003-06-04 2006-11-02 Bioleaders Corp Zelloberflächenexpressionsvektor von sars-virus-antigen und damit transformierte mikroorganismen
KR20050079162A (ko) * 2004-02-04 2005-08-09 주식회사 바이오리더스 파보바이러스 항원의 세포표면 발현벡터 및 상기 벡터에의해 형질전환된 미생물
US7255855B2 (en) 2004-02-27 2007-08-14 Bioleaders Corporation Surface expression method of peptides P5 and Anal3 using the gene encoding poly-gamma-glutamate synthetase
JP2005312426A (ja) * 2004-03-31 2005-11-10 Bioleaders Japan:Kk タンパク質を表層提示する大腸菌
JPWO2006117927A1 (ja) * 2005-03-04 2008-12-18 サントリー株式会社 ポリグルタミン酸を産生する形質転換植物
JP2006262724A (ja) * 2005-03-22 2006-10-05 Kobe Univ タンパク質高発現乳酸菌
WO2006126682A1 (ja) * 2005-05-27 2006-11-30 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute アルツハイマー病の予防・治療用ワクチン
KR100872042B1 (ko) * 2005-09-14 2008-12-05 주식회사 바이오리더스 마이오스타틴을 발현하는 세포표면 발현벡터 및 상기벡터에 의해 형질전환된 미생물
KR100578395B1 (ko) * 2005-11-11 2006-05-10 주식회사 바이오리더스 면역기능이 강화된 사균화 유산균 제제 및 그 제조방법
KR100782332B1 (ko) * 2006-01-23 2007-12-06 주식회사 바이오리더스 새우 흰 반점 바이러스 항원의 표면 발현벡터 및 이에 의해형질전환된 미생물
KR100985914B1 (ko) * 2006-12-13 2010-10-08 주식회사 바이오리더스 간암 특이 항원인 알파­페토단백질의 표면발현벡터 및 이에의해 형질전환된 미생물
KR100972843B1 (ko) 2007-03-22 2010-07-28 주식회사 바이오리더스 신규 항시적 고발현 프로모터 및 그 용도
KR100857861B1 (ko) 2007-10-15 2008-09-11 주식회사 바이오리더스 Ｍｙｏ－２ 펩타이드 중합체와 마이오스타틴의 융합단백질표면발현용 벡터 및 상기 벡터로 형질전환된 미생물
KR101564084B1 (ko) * 2009-01-08 2015-10-29 주식회사 바이오리더스 ｒｅｐＥ 변이유전자를 함유하는 항시적 고발현 벡터
KR101471043B1 (ko) 2009-01-08 2014-12-09 주식회사 바이오리더스 안정적인 항시적 고발현 자궁경부암 치료백신용 벡터 및 그에 의해 형질전환된 재조합 유산균
JP5669361B2 (ja) * 2009-03-17 2015-02-12 花王株式会社 ポリ−ガンマ−グルタミン酸の調整方法
JP5599153B2 (ja) * 2009-03-17 2014-10-01 花王株式会社 ポリ−ガンマ−グルタミン酸の製造方法
JP5561681B2 (ja) 2009-09-17 2014-07-30 森下仁丹株式会社 ビフィズス菌表層提示融合タンパク質発現遺伝子
JP5846523B2 (ja) * 2010-11-18 2016-01-20 国立大学法人神戸大学 βグルコシダーゼを発現する大腸菌
JP2014210747A (ja) 2013-04-19 2014-11-13 アンジェスＭｇ株式会社 細胞性免疫誘導能が改善された経口ワクチン
CN103555720B (zh) * 2013-11-19 2015-08-19 湖北工业大学 一种具有生产和回收纤维素内切酶双重功能的酵母菌株的构建
CN108350411B (zh) 2015-06-25 2021-06-22 白川利朗 口服肿瘤疫苗
JP6810877B2 (ja) 2016-12-26 2021-01-13 国立大学法人神戸大学 経口腫瘍ワクチンと免疫抑制阻害剤との併用によるがん治療
KR20200000972A (ko) 2018-06-26 2020-01-06 주식회사 바이오리더스 바실러스 속 균주 유래의 폴리감마글루탐산 합성유전자를 이용한 표면발현벡터 및 이를 이용한 단백질의 미생물표면 발현 방법
EP3940077B1 (de) * 2018-10-10 2025-01-01 Bioleaders Corporation Oberflächenexpressionsvektor mit zwei arten von promotoren aus lactobacillus casei zur gleichzeitigen expression zweier zielproteine und verfahren zur expression von proteinen auf einer mikrobiellen oberfläche unter verwendung desselben
JP7147060B2 (ja) * 2018-10-10 2022-10-04 バイオリーダース コーポレイション ラクトバチルス・カゼイ由来ガラクトース・ムタロターゼ遺伝子のプロモーターを用いた常時的高発現表面発現ベクターおよびその利用
CN111593029B (zh) * 2020-05-29 2022-05-17 江西省科学院微生物研究所 一种催化活性提高的γ-聚谷氨酸合成酶复合体及其编码基因
CN115725519B (zh) * 2021-04-28 2025-04-25 齐鲁工业大学(山东省科学院) γ-聚谷氨酸在植物中异源合成提高植物抗逆性和产量的方法
CN114702559A (zh) * 2022-04-02 2022-07-05 江西师范大学 枯草芽孢杆菌蛋白PgsA及其在表面展示系统中的应用
WO2024085480A1 (ko) 2022-10-17 2024-04-25 주식회사 퓨처앤텍 세포외막 지질단백질 PrsA 발현계를 이용한 세포표면 대량발현 기술
CN118956803A (zh) * 2022-11-22 2024-11-15 中国农业科学院茶叶研究所 茶树甲基化儿茶素合成酶CsFAOMT2、编码基因及其应用

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Also Published As

Publication number Publication date
CN1311082C (zh) 2007-04-18
KR20040032824A (ko) 2004-04-17
JP4458844B2 (ja) 2010-04-28
KR100469800B1 (ko) 2005-02-02
WO2003014360A1 (en) 2003-02-20
US20040253704A1 (en) 2004-12-16
CN1539018A (zh) 2004-10-20
DE60215521D1 (de) 2006-11-30
EP1421195A4 (de) 2005-04-27
EP1421195B1 (de) 2006-10-18
US7553636B2 (en) 2009-06-30
EP1421195A1 (de) 2004-05-26
JP2005500054A (ja) 2005-01-06
DE60215521T2 (de) 2007-06-21

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