ATE471381T1 - Expressionsvektor für tierische zelle mit wenigstens einer kopie von mar-dna-sequenzen am 3'-ende des transkriptionsterminationsbereichs eines gens sowie verfahren zur expression eines fremdgens unter verwendung des vektors - Google Patents
Expressionsvektor für tierische zelle mit wenigstens einer kopie von mar-dna-sequenzen am 3'-ende des transkriptionsterminationsbereichs eines gens sowie verfahren zur expression eines fremdgens unter verwendung des vektorsInfo
- Publication number
- ATE471381T1 ATE471381T1 AT06716203T AT06716203T ATE471381T1 AT E471381 T1 ATE471381 T1 AT E471381T1 AT 06716203 T AT06716203 T AT 06716203T AT 06716203 T AT06716203 T AT 06716203T AT E471381 T1 ATE471381 T1 AT E471381T1
- Authority
- AT
- Austria
- Prior art keywords
- gene
- vector
- expression vector
- expressing
- copy
- Prior art date
Links
- 239000013604 expression vector Substances 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 210000004102 animal cell Anatomy 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 title 1
- 230000005030 transcription termination Effects 0.000 title 1
- 239000013598 vector Substances 0.000 title 1
- 238000013518 transcription Methods 0.000 abstract 2
- 230000035897 transcription Effects 0.000 abstract 2
- 101000899111 Homo sapiens Hemoglobin subunit beta Proteins 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20050018130 | 2005-03-04 | ||
| PCT/KR2006/000753 WO2006093397A1 (en) | 2005-03-04 | 2006-03-04 | Expression vector for animal cell comprising at least one copy of mar dna sequences at the 3'terminal of transcription termination region of a gene and method for the expression of foreign gene using the vector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE471381T1 true ATE471381T1 (de) | 2010-07-15 |
Family
ID=36941411
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06716203T ATE471381T1 (de) | 2005-03-04 | 2006-03-04 | Expressionsvektor für tierische zelle mit wenigstens einer kopie von mar-dna-sequenzen am 3'-ende des transkriptionsterminationsbereichs eines gens sowie verfahren zur expression eines fremdgens unter verwendung des vektors |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8772021B2 (de) |
| EP (1) | EP1859046B1 (de) |
| KR (2) | KR20060096383A (de) |
| AT (1) | ATE471381T1 (de) |
| DE (1) | DE602006014942D1 (de) |
| WO (1) | WO2006093397A1 (de) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101607734B1 (ko) | 2010-10-08 | 2016-03-30 | 카딜라 핼쓰캐어 리미티드 | 재조합 단백질의 높은 레벨의 발현을 위한 발현 벡터 |
| US9062326B2 (en) * | 2011-03-30 | 2015-06-23 | Pangen Biotech Inc. | Expression vector for animal cells |
| KR101420274B1 (ko) * | 2011-06-13 | 2014-07-21 | 주식회사 종근당 | Ｃｓｐ－ｂ ５＇ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법 |
| CN102250952B (zh) * | 2011-06-17 | 2016-01-20 | 中国人民解放军军事医学科学院生物工程研究所 | 介导外源基因在哺乳动物细胞高效表达的载体及其应用 |
| KR20140015999A (ko) * | 2012-07-27 | 2014-02-07 | 한화케미칼 주식회사 | 신규 ＭＡＲｓ 및 이를 이용하여 목적 단백질을 생산하는 방법 |
| WO2016154055A1 (en) * | 2015-03-20 | 2016-09-29 | Bluebird Bio, Inc. | Vector formulations |
| US10722571B2 (en) | 2015-06-10 | 2020-07-28 | Celltrion Inc. | Rabies virus G protein epitope, and rabies virus neutralising binding molecule that binds specifically thereto |
| KR102735096B1 (ko) | 2017-10-11 | 2024-11-28 | (주)셀트리온 | 고발현 및 고기능성 목적 단백질의 생산을 위한 발현 카세트 및 이의 이용 |
| WO2019151632A1 (ko) | 2018-01-31 | 2019-08-08 | (주)셀트리온 | 중동호흡기증후군 코로나바이러스에 중화활성을 갖는 결합 분자 |
| WO2021062012A1 (en) | 2019-09-25 | 2021-04-01 | Emory University | Use of klk10 and engineered derivatizations thereof |
| KR102798616B1 (ko) | 2019-12-20 | 2025-04-22 | (주)셀트리온 | 목적 단백질의 고발현을 위한 인트론을 포함하는 발현 카세트 및 이의 이용 |
| KR102205028B1 (ko) | 2020-03-22 | 2021-01-20 | (주)셀트리온 | 사스-코로나바이러스-2에 중화 활성을 갖는 결합 분자 |
| WO2022019711A1 (ko) | 2020-07-24 | 2022-01-27 | (주)셀트리온 | 코로나바이러스 슈퍼패밀리에 중화 활성을 갖는 결합 분자 |
| KR102789747B1 (ko) | 2021-12-29 | 2025-04-03 | 한국과학기술원 | Prox1 단백질에 중화 활성을 갖는 결합 분자 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6245974B1 (en) * | 1997-08-06 | 2001-06-12 | North Carolina State University | Matrix attachment regions |
| US6177612B1 (en) * | 1998-07-31 | 2001-01-23 | Her Majesty The Queen In Right Of Canada, As Represented By The Department Of Agriculture And Agri-Food Canada | Matrix attachment regions |
| WO2000018938A1 (en) * | 1998-09-29 | 2000-04-06 | Pioneer Hi-Bred International, Inc. | Mar/sar elements flanking rsyn7-driven construct |
| GB2342282B (en) | 1998-10-08 | 2001-09-26 | Notetry Ltd | Changeover valve |
| KR100408844B1 (ko) | 2000-07-29 | 2003-12-06 | 한국산업기술평가원 | 동물세포 발현벡터 |
| WO2002048379A1 (en) | 2000-12-15 | 2002-06-20 | Pangen Biotech Inc. | Expression vector for animal cell containing nuclear matrix attachment region fo interferon beta |
| AU2002341795A1 (en) * | 2001-09-21 | 2003-04-01 | Avigenics, Inc. | Production of transgenic avians using sperm-mediated transfection |
| US6933136B2 (en) * | 2002-09-20 | 2005-08-23 | Novo Nordisk A/S | Method for making recombinant proteins |
-
2006
- 2006-03-04 AT AT06716203T patent/ATE471381T1/de not_active IP Right Cessation
- 2006-03-04 US US11/817,756 patent/US8772021B2/en active Active
- 2006-03-04 EP EP06716203A patent/EP1859046B1/de not_active Expired - Lifetime
- 2006-03-04 KR KR1020060020723A patent/KR20060096383A/ko not_active Ceased
- 2006-03-04 DE DE602006014942T patent/DE602006014942D1/de not_active Expired - Lifetime
- 2006-03-04 WO PCT/KR2006/000753 patent/WO2006093397A1/en not_active Ceased
-
2007
- 2007-10-26 KR KR1020070108451A patent/KR101076602B1/ko not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1859046A4 (de) | 2008-09-17 |
| US20080102523A1 (en) | 2008-05-01 |
| KR101076602B1 (ko) | 2011-10-26 |
| KR20070118563A (ko) | 2007-12-17 |
| EP1859046B1 (de) | 2010-06-16 |
| DE602006014942D1 (de) | 2010-07-29 |
| KR20060096383A (ko) | 2006-09-11 |
| WO2006093397A1 (en) | 2006-09-08 |
| EP1859046A1 (de) | 2007-11-28 |
| US8772021B2 (en) | 2014-07-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |