ATE344456T1 - Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen - Google Patents

Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen

Info

Publication number
ATE344456T1
ATE344456T1 AT01932999T AT01932999T ATE344456T1 AT E344456 T1 ATE344456 T1 AT E344456T1 AT 01932999 T AT01932999 T AT 01932999T AT 01932999 T AT01932999 T AT 01932999T AT E344456 T1 ATE344456 T1 AT E344456T1
Authority
AT
Austria
Prior art keywords
protein
epptides
affinity
identifying
selected signature
Prior art date
Application number
AT01932999T
Other languages
English (en)
Inventor
Fred E Regnier
Asish B Chakraborty
Xiang Zhang
Original Assignee
Purdue Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation filed Critical Purdue Research Foundation
Application granted granted Critical
Publication of ATE344456T1 publication Critical patent/ATE344456T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT01932999T 2000-05-05 2001-05-04 Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen ATE344456T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20322700P 2000-05-05 2000-05-05
US20837200P 2000-05-31 2000-05-31
US20818400P 2000-05-31 2000-05-31

Publications (1)

Publication Number Publication Date
ATE344456T1 true ATE344456T1 (de) 2006-11-15

Family

ID=27394529

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01932999T ATE344456T1 (de) 2000-05-05 2001-05-04 Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen

Country Status (9)

Country Link
US (5) US6864099B2 (de)
EP (1) EP1311858B1 (de)
JP (1) JP2003532900A (de)
AT (1) ATE344456T1 (de)
AU (3) AU2001259470A1 (de)
CA (1) CA2402871C (de)
DE (1) DE60124265D1 (de)
IL (2) IL152620A (de)
WO (1) WO2001086306A2 (de)

Families Citing this family (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5910403A (en) * 1997-05-15 1999-06-08 The Regents Of University Of California Methods for measuring cellular proliferation and destruction rates in vitro and in vivo
ATE344456T1 (de) * 2000-05-05 2006-11-15 Purdue Research Foundation Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen
US6806057B2 (en) * 2000-10-27 2004-10-19 The Johns Hopkins University Method for assaying protein nitrosylation
US20020090652A1 (en) * 2000-12-22 2002-07-11 Fu Emil Wei-Ming Inverse labeling method for the rapid identification of marker/target proteins
WO2002086168A1 (en) * 2001-04-19 2002-10-31 Ciphergen Biosystems, Inc. Biomolecule characterization using mass spectrometry and affinity tags
EP1432992A2 (de) 2001-09-27 2004-06-30 Purdue Research Foundation Kontrolle von isotopeneffekten während der analytfraktionierung
US20030077572A1 (en) * 2001-10-11 2003-04-24 Abramson Fred P. Quantitative differential display using isotope ratio monitoring
WO2003061479A1 (en) * 2001-10-24 2003-07-31 The Regents Of The University Of California Measurement of protein synthesis rates in humans and experimental systems by use of isotopically labeled water
ATE412906T1 (de) * 2002-02-12 2008-11-15 Univ California Nichtinvasive messung der biosynthese- und abbaugeschwindigkeiten biologischer moleküle, die einer direkten probenahme unzugänglich oder nicht leicht zugänglich sind, durch einbau einer markierung in metabolische derivate und katabole produkte
AU2003234688A1 (en) * 2002-04-05 2003-10-27 The Regents Of The University Of California Method for isolating and measuring proliferation of long-term label retaining cells and stem cells
JP4895504B2 (ja) * 2002-04-30 2012-03-14 ユィロス・パテント・アクチボラグ 集中マイクロ流体デバイス（ｅａ）
ATE381018T1 (de) * 2002-05-10 2007-12-15 Proteome Factory Ag Festphasenassisitierte spektroskopische und spektrometrische analyse komplexer biopolymergemische
US20070269895A1 (en) * 2002-06-03 2007-11-22 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
WO2003102018A2 (en) * 2002-06-03 2003-12-11 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
DE10227599B4 (de) * 2002-06-20 2005-04-28 Proteome Factory Ag Verfahren und Reagenz zur spezifischen Identifizierung und Quantifizierung von einem oder mehreren Proteinen in einer Probe
FR2842826B1 (fr) * 2002-07-25 2006-01-20 Centre Nat Rech Scient Procede de fabrication de puces biologiques
US20060094057A1 (en) 2002-07-30 2006-05-04 Maro K. Hellerstein Method for automated, large-scale measurement of the molecular flux rates of the proteome or the organeome using mass spectrometry
AU2003259707A1 (en) * 2002-08-12 2004-02-25 Cornell Research Foundation, Inc. Mass spectrometry-based identification of proteins
WO2004021863A2 (en) * 2002-09-04 2004-03-18 The Regents Of The University Of California Methods for measuring rates of replication and death of nfectious microbial agents in an infected host organism
JP4610337B2 (ja) * 2002-09-13 2011-01-12 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 抗アテローム発生の指標としてのｉｎｖｉｖｏでの逆コレステロール輸送速度の測定方法
US20070248540A1 (en) * 2002-09-16 2007-10-25 The Regents Of The University Of California Biochemical methods for measuring metabolic fitness of tissues or whole organisms
HK1079707A1 (zh) * 2002-09-16 2006-04-13 The Regents Of The University Of California 测量组织或整个生物的代谢适应性的生化方法
EP2186879B1 (de) 2002-10-03 2016-11-23 Norman Leigh Anderson Hochempfindliche Quantifizierung von Peptiden mittels Massenspektrometrie
US7632686B2 (en) 2002-10-03 2009-12-15 Anderson Forschung Group High sensitivity quantitation of peptides by mass spectrometry
DE10247896B4 (de) * 2002-10-14 2007-01-18 Bruker Daltonik Gmbh Mehrdimensionale Trennung von Biosubstanzgemischen für massenspektrometrische Analysen
AU2003286553A1 (en) * 2002-10-18 2004-05-04 Promega Corporation Methods for separating molecules
US7544932B2 (en) * 2002-10-21 2009-06-09 The United States Of America, As Represented By The Secretary, Of The Department Of Health And Human Services Contiguous capillary electrospray sources and analytical devices
WO2004042360A2 (en) * 2002-11-04 2004-05-21 The Regents Of The Univeristy Of California Deuterated glucose or fat tolerance tests for high-throughput measurement of the metabolism of sugars or fatty acids in the body
US7109040B2 (en) * 2002-12-13 2006-09-19 Agilent Technologies, Inc. Modular isotope coding approach to proteomic analysis
US7425451B2 (en) 2002-12-13 2008-09-16 Agilent Technologies, Inc. Triazine derivatives as universal peptide isotope tag reagents (U-PIT)
US7422865B2 (en) * 2003-01-13 2008-09-09 Agilent Technologies, Inc. Method of identifying peptides in a proteomic sample
JP2004301715A (ja) * 2003-03-31 2004-10-28 Institute Of Physical & Chemical Research タンパク質の一次構造情報収集方法、それに用いる疎水性微細粒子担体及びタンパク質一次構造情報自動取得システム
US7262020B2 (en) * 2003-07-03 2007-08-28 The Regents Of The University Of California Methods for comparing relative flux rates of two or more biological molecules in vivo through a single protocol
JP2007502837A (ja) * 2003-08-18 2007-02-15 テシス バイオサイエンス， インコーポレイテッド 小型エピトープ抗体を使用するサンプルの複雑さを低減するための方法
US20050202406A1 (en) * 2003-11-25 2005-09-15 The Regents Of The University Of California Method for high-throughput screening of compounds and combinations of compounds for discovery and quantification of actions, particularly unanticipated therapeutic or toxic actions, in biological systems
JP2005189104A (ja) * 2003-12-25 2005-07-14 Shimadzu Corp タンパク質又はペプチドの濃縮分離法
EP1711833A2 (de) * 2004-02-06 2006-10-18 Applera Corporation Herstellung biologisch abgeleiteter fluide zur biomarkerbestimmung mittels massenspektrometrie
WO2005075993A1 (en) * 2004-02-10 2005-08-18 Korea Basic Science Institute Selective labeling agent for phosphoproteome analysis and phosphorylated site analysis
US20070087448A1 (en) * 2004-02-16 2007-04-19 Nelsestuen Gary L Biological profiles and methods of use
TW200538738A (en) 2004-02-20 2005-12-01 Univ California Molecular flux rates through critical pathways measured by stable isotope labeling in vivo, as biomarkers of drug action and disease activity
US20050201937A1 (en) * 2004-03-11 2005-09-15 The Regents Of The University Of California Temporal or spatial characterization of biosynthetic events in living organisms by isotopic fingerprinting under conditions of imposed isotopic gradients
WO2005094327A2 (en) * 2004-03-29 2005-10-13 The Regents Of The University Of California Isolation of epithelial cells or their biochemical contents from excreta after in vivo isotopic labeling
CA2567459A1 (en) * 2004-05-19 2005-12-01 Applera Corporation Expression quantification using mass spectrometry
US20070054345A1 (en) * 2004-05-19 2007-03-08 Hunter Christie L Expression quantification using mass spectrometry
US20080206737A1 (en) * 2004-05-19 2008-08-28 Hunter Christie L Expression quantification using mass spectrometry
US20060154318A1 (en) * 2004-06-09 2006-07-13 Anderson Norman L Stable isotope labeled polypeptide standards for protein quantitation
KR100611313B1 (ko) * 2004-06-30 2006-08-10 고려대학교 산학협력단 폴리펩티드의 ｎ-말단 치환용 화합물, 이를 이용한폴리펩티드 내의 아미노산 서열분석 및 정량방법
SE0401854D0 (sv) * 2004-07-09 2004-07-09 Amersham Biosciences Ab Method and kit for peptide analysis
WO2006037059A2 (en) * 2004-09-27 2006-04-06 The Johns Hopkins Universtiy Point-of-care mass spectrometer system
US20060251576A1 (en) * 2005-05-03 2006-11-09 The Regents Of The University Of California Methods for measuring cholesterol metabolism and transport
DE602005004902T2 (de) * 2005-06-02 2008-10-16 Entelechon Gmbh Künstliches Protein, Verfahren für absolute Quantifizierung der Proteine und seine Benutzung
TW200711660A (en) 2005-06-10 2007-04-01 Univ California Monitoring two dimensions of diabetes pathogenesis separately or concurrently (insulin sensitivity and beta-cell sufficiency): uses in diagnosis, prognosis, assessment of disease risk, and drug development
AU2006291835B2 (en) 2005-09-15 2012-04-05 Alk-Abello A/S A method for quantification of allergens
EP1941280A2 (de) * 2005-10-13 2008-07-09 Applera Corporation Verfahren zur entwicklung eines biomolekültests
EP1785729A3 (de) * 2005-10-17 2007-06-13 Centro De Ingenieria Genetica Y Biotecnologia (Cigb) Verfahren zur selektiven Isolation von in der quantitativen Proteomik anwendbaren mehrfach-geladenen Peptiden
US20070128729A1 (en) * 2005-11-22 2007-06-07 Puente Aniel S Method for the identification and relative quantification of proteins based on the selective isolation of RRnK peptides for the simplification of complex mixtures of proteins
CN100465640C (zh) * 2005-11-30 2009-03-04 上海特敏生物医药科技有限公司 一种高灵敏血浆总同型半胱氨酸测定试剂盒
WO2007078229A1 (en) * 2006-01-05 2007-07-12 Ge Healthcare Bio-Sciences Ab Kit and method for mass labelling
DE102006001793A1 (de) * 2006-01-12 2007-07-26 Forschungsgesellschaft für Arbeitsphysiologie und Arbeitsschutz e.V. Verfahren und diagnostisches Kit zur simultanen Identifizierung und Quantifizierung von einzelnen oder in Gemischen vorliegenden Biomolekülen und deren Modifikationen
US20080026480A1 (en) * 2006-02-14 2008-01-31 Perkinelmer Las, Inc. Detection systems for mass labels
EP1996942A1 (de) 2006-03-10 2008-12-03 Tethys Bioscience, Inc. Multiplex-protein-fraktionierung
EP2021492A2 (de) * 2006-04-28 2009-02-11 Ludwig Institute For Cancer Research Quantifizierung von enzymaktivitäten durch massenspektrometrie
CN101101283A (zh) * 2006-07-05 2008-01-09 中国科学院大连化学物理研究所 一种分离富集磷酸化肽的方法
WO2008141358A1 (en) * 2007-05-17 2008-11-27 Proteomics International Pty Ltd Fragmentation profile based authentification of protein source
WO2008150763A1 (en) * 2007-05-29 2008-12-11 Waters Investments Limited Apparatus and methods for multidimensional analysis
JP4836875B2 (ja) * 2007-06-13 2011-12-14 株式会社日立ハイテクノロジーズ 修飾ペプチドの分析方法および装置
WO2009006382A1 (en) * 2007-07-02 2009-01-08 Purdue Research Foundation Detection of glycopeptides and glycoproteins for medical diagnostics
EP2210109A1 (de) * 2007-10-19 2010-07-28 Pronota NV Herstellung von proben zur proteomanalyse
JPWO2009123347A1 (ja) * 2008-03-31 2011-07-28 日本たばこ産業株式会社 ウイルス定量法
WO2009134439A2 (en) * 2008-05-02 2009-11-05 Purdue Research Foundation Group specific internal standard technology (gsist) for simultaneous identification and quantification of small molecules
WO2010109022A1 (en) * 2009-03-27 2010-09-30 Universitetet I Oslo Quantitative proteomics method
US8592216B2 (en) * 2009-04-15 2013-11-26 Wisconsin Alumni Research Foundation Labeling peptides with tertiary amines and other basic functional groups for improved mass spectrometric analysis
WO2011072197A2 (en) 2009-12-11 2011-06-16 Purdue Research Foundation Detection of oxidized polypeptides
US8383417B2 (en) * 2009-12-22 2013-02-26 Thermo Finnigan, Llc Assay for monitoring parathyroid hormone (PTH) variants by tandem mass spectrometry
WO2011109832A2 (en) * 2010-03-05 2011-09-09 The Salk Institute For Biological Studies Methods and compositions for nmr spectroscopic analysis using isotopic labeling schemes
AU2010347764B2 (en) * 2010-03-10 2013-11-14 Perfinity Biosciences, Inc. Method for recognition and quantification of multiple analytes in a single analysis
US8455202B2 (en) 2010-03-10 2013-06-04 Perfinity Biosciences, Inc. Affinity selector based recognition and quantification system and method for multiple analytes in a single analysis
US8772043B2 (en) 2010-12-23 2014-07-08 Pioneer Hi Bred International Inc Universal peptide tags for transgene polypeptide analysis by mass spectrometry
WO2013036885A1 (en) 2011-09-08 2013-03-14 The Regents Of The University Of California Metabolic flux measurement, imaging and microscopy
ES2668678T3 (es) 2011-12-07 2018-05-21 Glaxosmithkline Llc Procedimiento de determinación de la masa muscular esquelética corporal total
WO2013094579A1 (ja) * 2011-12-20 2013-06-27 日本ケミカルリサーチ株式会社 ホルミルグリシン残基の分析法
US9048072B2 (en) * 2012-03-12 2015-06-02 Micromass Uk Limited Method of mass spectrometry and a mass spectrometer
GB201204723D0 (en) * 2012-03-19 2012-05-02 Micromass Ltd Improved time of flight quantitation using alternative characteristic ions
US10145818B2 (en) 2012-10-22 2018-12-04 President And Fellows Of Harvard College Accurate and interference-free multiplexed quantitative proteomics using mass spectrometry
EP2951300B1 (de) 2013-01-31 2020-03-04 Perfinity Biosciences, Inc. Robuste, benutzerfreundliche immobilisierte enzymreaktoren
US9134319B2 (en) 2013-03-15 2015-09-15 The Regents Of The University Of California Method for replacing biomarkers of protein kinetics from tissue samples by biomarkers of protein kinetics from body fluids after isotopic labeling in vivo
JP6079587B2 (ja) * 2013-11-27 2017-02-15 株式会社島津製作所 糖ペプチド解析装置
US10551358B2 (en) * 2013-12-03 2020-02-04 Perfinity Bioscices, Inc. Single reactor for simplified sample preparation workflows
US10018635B2 (en) 2014-07-30 2018-07-10 Novilytic, LLC Method for analyzing samples of a biological fluid
US9786478B2 (en) 2014-12-05 2017-10-10 Purdue Research Foundation Zero voltage mass spectrometry probes and systems
EP3254297B1 (de) 2015-02-06 2024-04-03 Purdue Research Foundation Sonden, systeme und kartuschen
WO2016164530A1 (en) * 2015-04-07 2016-10-13 University Of Maryland, College Park Compositions and methods for high throughput protein sequencing
EP3304090A4 (de) * 2015-05-29 2018-11-07 Cedars-Sinai Medical Center Korrelierte peptide für quantitative massenspektrometrie
US11085927B2 (en) 2016-06-03 2021-08-10 President And Fellows Of Harvard College Techniques for high throughput targeted proteomic analysis and related systems and methods
EP3734268B1 (de) * 2017-12-28 2022-07-13 Shimadzu Corporation Vereinfachtes verfahren zur quantifizierung von monoklonalen antikörpern
EA202192036A1 (ru) * 2019-01-25 2021-10-18 Ридженерон Фармасьютикалз, Инк. Онлайн хроматография и масс-спектрометр с ионизацией электрораспылением
EP3892989A1 (de) * 2020-04-07 2021-10-13 Ares Trading S.A. Kapillargelelektrophorese und ihre verwendung mit komplexen biologischen molekülen
CN115667313A (zh) * 2020-06-11 2023-01-31 快速诺凡公司 抗体测序的试剂和方法
JP7667684B2 (ja) * 2021-04-06 2025-04-23 株式会社日立製作所 検体の分析前処理方法および検体前処理装置
CN113804897A (zh) * 2021-09-17 2021-12-17 中国食品药品检定研究院 一种冬虫夏草及各发酵虫草制剂特征肽段的序列鉴定方法
US20240036055A1 (en) * 2022-06-15 2024-02-01 Wisconsin Alumni Research Foundation Multiplexed DiLeu-Biotin-Azide (DBA) Tag Enabled Isobaric Tandem Orthogonal Proteolysis Activity-Based Protein Profiling (isoBOP-ABPP) Platform For High-Throughput Quantitative Pan-PTM Analysis
CN117030899A (zh) * 2023-08-28 2023-11-10 中信湘雅生殖与遗传专科医院有限公司 蛋白样品的处理方法及蛋白组分析方法
WO2025057852A1 (ja) * 2023-09-14 2025-03-20 株式会社島津製作所 代謝物の分析前処理方法
CN117347531B (zh) * 2023-12-05 2024-02-13 天津医科大学总医院 一种采用双重内标定量检测蛋白质的方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340966C (en) * 1989-05-19 2000-04-18 Thomas R. Covey Method of protein analysis
US5652128A (en) 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins
US5393669A (en) * 1993-02-05 1995-02-28 Martek Biosciences Corp. Compositions and methods for protein structural determinations
ES2205437T3 (es) * 1997-01-23 2004-05-01 XZILLION GMBH & CO.KG Procedimiento que permite caracterizar polipeptidos.
EP1105731B1 (de) 1998-08-12 2006-10-18 Zycos Inc. Darstellung des profils und katalogisierung von exprimierten proteinmarkern
EP1329513B1 (de) * 1998-08-25 2011-12-07 University of Washington Schnelle quantitative Analyse von Proteinen oder Proteinfunktionen in komplexen Gemischen
GB9821669D0 (en) * 1998-10-05 1998-12-02 Glaxo Group Ltd Chemical constructs and their uses
US6629040B1 (en) * 1999-03-19 2003-09-30 University Of Washington Isotope distribution encoded tags for protein identification
US6391649B1 (en) * 1999-05-04 2002-05-21 The Rockefeller University Method for the comparative quantitative analysis of proteins and other biological material by isotopic labeling and mass spectroscopy
ATE344456T1 (de) * 2000-05-05 2006-11-15 Purdue Research Foundation Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen
AU6689401A (en) * 2000-06-12 2001-12-24 Univ Washington Selective labeling and isolation of phosphopeptides and applications to proteomeanalysis
CA2426728A1 (en) 2000-10-23 2002-06-13 Genetics Institute, Llc. Isotope-coded ionization-enhancing reagents (icier) for high-throughput protein identification and quantitation using matrix-assisted laser desorption ionization mass spectrometry
US20020119490A1 (en) * 2000-12-26 2002-08-29 Aebersold Ruedi H. Methods for rapid and quantitative proteome analysis
US7045296B2 (en) * 2001-05-08 2006-05-16 Applera Corporation Process for analyzing protein samples
US7183116B2 (en) * 2001-05-14 2007-02-27 The Institute For Systems Biology Methods for isolation and labeling of sample molecules
SE0101683D0 (sv) * 2001-05-14 2001-05-14 Torkel Klingberg New method
EP1432992A2 (de) * 2001-09-27 2004-06-30 Purdue Research Foundation Kontrolle von isotopeneffekten während der analytfraktionierung
US6970630B2 (en) * 2002-05-23 2005-11-29 Rutgers, The State University Of New Jersey Fiber optic cable and process for manufacturing
WO2003102018A2 (en) * 2002-06-03 2003-12-11 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
US20070269895A1 (en) * 2002-06-03 2007-11-22 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
US7655433B2 (en) * 2002-06-04 2010-02-02 The Institute For Systems Biology Methods for high-throughput and quantitative proteome analysis
US7473535B2 (en) * 2002-08-20 2009-01-06 The Institute For Systems Biology Chemical reagents and methods for detection and quantification of proteins in complex mixtures
WO2005007677A2 (en) * 2003-07-10 2005-01-27 The Institute For Systems Biology Affinity capture of peptides by microarray and related methods
ATE427499T1 (de) * 2004-05-21 2009-04-15 Inst Systems Biology Zusammensetzungen und verfahren zur quantifizierung von serumglykoproteinen
US20070099251A1 (en) * 2005-10-17 2007-05-03 Institute For Systems Biology Tissue-and serum-derived glycoproteins and methods of their use

Also Published As

Publication number Publication date
US20020037532A1 (en) 2002-03-28
IL152620A (en) 2010-05-17
US6864099B2 (en) 2005-03-08
IL152620A0 (en) 2003-06-24
AU2005209637B2 (en) 2008-06-12
JP2003532900A (ja) 2003-11-05
DE60124265D1 (de) 2006-12-14
EP1311858B1 (de) 2006-11-02
AU2008213716A1 (en) 2008-10-02
US20030129769A1 (en) 2003-07-10
US20080145863A1 (en) 2008-06-19
WO2001086306A3 (en) 2003-03-13
CA2402871C (en) 2011-08-23
WO2001086306A2 (en) 2001-11-15
EP1311858A2 (de) 2003-05-21
CA2402871A1 (en) 2001-11-15
US6872575B2 (en) 2005-03-29
US20100273178A1 (en) 2010-10-28
IL200933A (en) 2011-04-28
AU2005209637A1 (en) 2005-09-29
AU2008213716B2 (en) 2012-03-15
AU2001259470A1 (en) 2001-11-20
US20030092076A1 (en) 2003-05-15

Similar Documents

Publication Publication Date Title
DE60124265D1 (de) Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen
Orvisky et al. Enrichment of low molecular weight fraction of serum for MS analysis of peptides associated with hepatocellular carcinoma
DE60217328D1 (de) Verfahren zur analyse von proteinproben
JP2003532900A5 (de)
WO2002042427A3 (en) Mass tags for quantitative analysis
DE60029572D1 (de) Isolierung und analyse von proteinen
DE60239962D1 (de) Absolute quantifizierung von proteinen und modifizierten formen davon mittels mehrstufiger massenspektrometrie
WO2004031730A3 (en) High sensitivity quantitation of peptides by mass spectrometry
ATE377756T1 (de) Verfahren und gerät für die identifikation und zuordnung von biomolekülen
WO2001084140A3 (de) Verfahren und vorrichtung zur qualitativen und/oder quantitativen bestimmung eines protein- und/oder peptidmusters einer flüssigkeitsprobe, die dem menschlichen oder tierischen körper entnommen wird
ATE411398T1 (de) Massenspektrometrische analyse von biopolymeren
WO2003102220A3 (en) Methods for high throughput and quantitative proteome analysis
WO2002008767A3 (en) Methods and kits for sequencing polypeptides
WO2004111636A3 (en) Peptide combos and their uses
DE60129239D1 (de) Kit und verfahren zur bestimmung von protein esm-1
ATE343137T1 (de) Verfahren zur bestimmung von polypeptiden
TW200420491A (en) Integrated microfluidic electro-spray chip system and the analysis method thereof
JP2019088213A (ja) コラーゲン由来材料の動物種を判定する方法
DE50105263D1 (de) Verfahren zur analyse von proteinen
DK0960336T3 (da) Peptidreagens til påvisning af humant cytomelagovirus (CMV)
ATE274190T1 (de) Methoden und kits zum sequenzieren von polypeptiden
WO2003056343B1 (en) Method for protein expression analysis
EP1589341A3 (de) Methode zur selektiven Isolierung von Peptiden zwecks Identifizierung und quantitativer Analyse der Proteine in komplexen Mischungen
WO2000007024A3 (en) Method of protein analysis
WO2008070570A3 (en) Positive selection of serum proteins for proteomic analysis

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties