ATE344456T1 - Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen - Google Patents

Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen

Info

Publication number
ATE344456T1
ATE344456T1 AT01932999T AT01932999T ATE344456T1 AT E344456 T1 ATE344456 T1 AT E344456T1 AT 01932999 T AT01932999 T AT 01932999T AT 01932999 T AT01932999 T AT 01932999T AT E344456 T1 ATE344456 T1 AT E344456T1
Authority
AT
Austria
Prior art keywords
protein
epptides
affinity
identifying
selected signature
Prior art date
Application number
AT01932999T
Other languages
English (en)
Inventor
Fred E Regnier
Asish B Chakraborty
Xiang Zhang
Original Assignee
Purdue Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation filed Critical Purdue Research Foundation
Application granted granted Critical
Publication of ATE344456T1 publication Critical patent/ATE344456T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848Methods of protein analysis involving mass spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848Methods of protein analysis involving mass spectrometry
    • G01N33/6851Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/24Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/25Chemistry: analytical and immunological testing including sample preparation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT01932999T 2000-05-05 2001-05-04 Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen ATE344456T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20322700P 2000-05-05 2000-05-05
US20837200P 2000-05-31 2000-05-31
US20818400P 2000-05-31 2000-05-31

Publications (1)

Publication Number Publication Date
ATE344456T1 true ATE344456T1 (de) 2006-11-15

Family

ID=27394529

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01932999T ATE344456T1 (de) 2000-05-05 2001-05-04 Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen

Country Status (9)

Country Link
US (5) US6864099B2 (de)
EP (1) EP1311858B1 (de)
JP (1) JP2003532900A (de)
AT (1) ATE344456T1 (de)
AU (3) AU2001259470A1 (de)
CA (1) CA2402871C (de)
DE (1) DE60124265D1 (de)
IL (2) IL152620A (de)
WO (1) WO2001086306A2 (de)

Families Citing this family (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5910403A (en) * 1997-05-15 1999-06-08 The Regents Of University Of California Methods for measuring cellular proliferation and destruction rates in vitro and in vivo
EP1311858B1 (de) 2000-05-05 2006-11-02 Purdue Research Foundation Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen
DE60118621T2 (de) * 2000-10-27 2007-05-03 The Johns Hopkins University Verfahren zur bestimmung der nitrosylierung von proteinen
US20020090652A1 (en) * 2000-12-22 2002-07-11 Fu Emil Wei-Ming Inverse labeling method for the rapid identification of marker/target proteins
CN1463291A (zh) * 2001-04-19 2003-12-24 赛弗根生物系统股份有限公司 用质谱法和亲和标记物鉴定生物分子的特性
WO2003027682A2 (en) * 2001-09-27 2003-04-03 Purdue Research Foundation Controlling isotope effects during fractionation of analytes
US20030077572A1 (en) * 2001-10-11 2003-04-24 Abramson Fred P. Quantitative differential display using isotope ratio monitoring
ATE502578T1 (de) * 2001-10-24 2011-04-15 Univ California Messung der protein-syntheseraten bei menschen und in experimentellen systemen durch verwendung von isotopisch markiertem wasser
AU2003217385B2 (en) * 2002-02-12 2008-09-11 The Regents Of The University Of California Non-invasive method for measuring rates of biosynthesis of biological molecules by label incorporation
CA2503681A1 (en) * 2002-04-05 2003-10-23 Marc K. Hellerstein Method for isolating and measuring proliferation of long-term label retaining cells and stem cells
AU2003224586A1 (en) * 2002-04-30 2003-11-17 Gyros Ab Integrated microfluidic device (ea)
ATE381018T1 (de) * 2002-05-10 2007-12-15 Proteome Factory Ag Festphasenassisitierte spektroskopische und spektrometrische analyse komplexer biopolymergemische
EP1514107B1 (de) * 2002-06-03 2013-05-15 The Institute for Systems Biology Verfahren zur quantitativen proteomanalyse von glycoproteinen
US20070269895A1 (en) * 2002-06-03 2007-11-22 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
DE10227599B4 (de) * 2002-06-20 2005-04-28 Proteome Factory Ag Verfahren und Reagenz zur spezifischen Identifizierung und Quantifizierung von einem oder mehreren Proteinen in einer Probe
FR2842826B1 (fr) * 2002-07-25 2006-01-20 Centre Nat Rech Scient Procede de fabrication de puces biologiques
EP1546364A4 (de) 2002-07-30 2006-09-06 Univ California Verfahren zur automatischen messung im grossmassstab der molekularen flussgeschwindigkeiten des proteoms oder des organoms mittels massenspektrometrie
WO2004015391A2 (en) * 2002-08-12 2004-02-19 Cornell Research Foundation, Inc. Mass spectrometry-based identification of proteins
US20060105339A1 (en) * 2002-09-04 2006-05-18 Marc Hellerstein Methods for measuring the rates of replication and death of microbial infectious agents in an infected
CA2498378C (en) * 2002-09-13 2013-12-17 The Regents Of The University Of California Methods for measuring rates of reverse cholesterol transport in vivo, as an index of anti-atherogenesis
SG152923A1 (en) * 2002-09-16 2009-06-29 Univ California Biochemical methods for measuring metabolic fitness of tissues or whole organisms
US20070248540A1 (en) * 2002-09-16 2007-10-25 The Regents Of The University Of California Biochemical methods for measuring metabolic fitness of tissues or whole organisms
EP2186879B1 (de) 2002-10-03 2016-11-23 Norman Leigh Anderson Hochempfindliche Quantifizierung von Peptiden mittels Massenspektrometrie
US7632686B2 (en) 2002-10-03 2009-12-15 Anderson Forschung Group High sensitivity quantitation of peptides by mass spectrometry
DE10247896B4 (de) * 2002-10-14 2007-01-18 Bruker Daltonik Gmbh Mehrdimensionale Trennung von Biosubstanzgemischen für massenspektrometrische Analysen
US7354750B2 (en) * 2002-10-18 2008-04-08 Promega Corporation Methods for separating molecules
WO2004038752A2 (en) * 2002-10-21 2004-05-06 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Contiguous capillary electrospray sources and analytical device
WO2004042360A2 (en) 2002-11-04 2004-05-21 The Regents Of The Univeristy Of California Deuterated glucose or fat tolerance tests for high-throughput measurement of the metabolism of sugars or fatty acids in the body
US7109040B2 (en) * 2002-12-13 2006-09-19 Agilent Technologies, Inc. Modular isotope coding approach to proteomic analysis
US7425451B2 (en) 2002-12-13 2008-09-16 Agilent Technologies, Inc. Triazine derivatives as universal peptide isotope tag reagents (U-PIT)
US7422865B2 (en) * 2003-01-13 2008-09-09 Agilent Technologies, Inc. Method of identifying peptides in a proteomic sample
JP2004301715A (ja) * 2003-03-31 2004-10-28 Institute Of Physical & Chemical Research タンパク質の一次構造情報収集方法、それに用いる疎水性微細粒子担体及びタンパク質一次構造情報自動取得システム
US7262020B2 (en) * 2003-07-03 2007-08-28 The Regents Of The University Of California Methods for comparing relative flux rates of two or more biological molecules in vivo through a single protocol
AU2004267802B2 (en) * 2003-08-18 2011-03-17 Tethys Bioscience, Inc. Methods for reducing complexity of a sample using small epitope antibodies
US20050202406A1 (en) * 2003-11-25 2005-09-15 The Regents Of The University Of California Method for high-throughput screening of compounds and combinations of compounds for discovery and quantification of actions, particularly unanticipated therapeutic or toxic actions, in biological systems
JP2005189104A (ja) * 2003-12-25 2005-07-14 Shimadzu Corp タンパク質又はペプチドの濃縮分離法
EP1711833A2 (de) * 2004-02-06 2006-10-18 Applera Corporation Herstellung biologisch abgeleiteter fluide zur biomarkerbestimmung mittels massenspektrometrie
WO2005075993A1 (en) * 2004-02-10 2005-08-18 Korea Basic Science Institute Selective labeling agent for phosphoproteome analysis and phosphorylated site analysis
US20070087448A1 (en) * 2004-02-16 2007-04-19 Nelsestuen Gary L Biological profiles and methods of use
TW200538738A (en) 2004-02-20 2005-12-01 Univ California Molecular flux rates through critical pathways measured by stable isotope labeling in vivo, as biomarkers of drug action and disease activity
CA2559095A1 (en) * 2004-03-11 2005-09-22 The Regents Of The University Of California Temporal or spatial characterization of biosynthetic events in living organisms by isotopic fingerprinting under conditions of imposed isotopic gradients
WO2005094327A2 (en) * 2004-03-29 2005-10-13 The Regents Of The University Of California Isolation of epithelial cells or their biochemical contents from excreta after in vivo isotopic labeling
WO2005114700A2 (en) * 2004-05-19 2005-12-01 Applera Corporation Expression quantification using mass spectrometry
US20070054345A1 (en) * 2004-05-19 2007-03-08 Hunter Christie L Expression quantification using mass spectrometry
US20080206737A1 (en) * 2004-05-19 2008-08-28 Hunter Christie L Expression quantification using mass spectrometry
US20060154318A1 (en) * 2004-06-09 2006-07-13 Anderson Norman L Stable isotope labeled polypeptide standards for protein quantitation
KR100611313B1 (ko) * 2004-06-30 2006-08-10 고려대학교 산학협력단 폴리펩티드의 n-말단 치환용 화합물, 이를 이용한폴리펩티드 내의 아미노산 서열분석 및 정량방법
SE0401854D0 (sv) * 2004-07-09 2004-07-09 Amersham Biosciences Ab Method and kit for peptide analysis
WO2006037059A2 (en) * 2004-09-27 2006-04-06 The Johns Hopkins Universtiy Point-of-care mass spectrometer system
US20060251576A1 (en) * 2005-05-03 2006-11-09 The Regents Of The University Of California Methods for measuring cholesterol metabolism and transport
DE602005004902T2 (de) * 2005-06-02 2008-10-16 Entelechon Gmbh Künstliches Protein, Verfahren für absolute Quantifizierung der Proteine und seine Benutzung
TW200711660A (en) 2005-06-10 2007-04-01 Univ California Monitoring two dimensions of diabetes pathogenesis separately or concurrently (insulin sensitivity and beta-cell sufficiency): uses in diagnosis, prognosis, assessment of disease risk, and drug development
PL1931998T3 (pl) 2005-09-15 2010-11-30 Alk Abello As Sposób ilościowego oznaczania alergenów
EP1941280A2 (de) * 2005-10-13 2008-07-09 Applera Corporation Verfahren zur entwicklung eines biomolekültests
EP1785729A3 (de) * 2005-10-17 2007-06-13 Centro De Ingenieria Genetica Y Biotecnologia (Cigb) Verfahren zur selektiven Isolation von in der quantitativen Proteomik anwendbaren mehrfach-geladenen Peptiden
US20070128729A1 (en) * 2005-11-22 2007-06-07 Puente Aniel S Method for the identification and relative quantification of proteins based on the selective isolation of RRnK peptides for the simplification of complex mixtures of proteins
CN100465640C (zh) * 2005-11-30 2009-03-04 上海特敏生物医药科技有限公司 一种高灵敏血浆总同型半胱氨酸测定试剂盒
WO2007078229A1 (en) * 2006-01-05 2007-07-12 Ge Healthcare Bio-Sciences Ab Kit and method for mass labelling
DE102006001793A1 (de) * 2006-01-12 2007-07-26 Forschungsgesellschaft für Arbeitsphysiologie und Arbeitsschutz e.V. Verfahren und diagnostisches Kit zur simultanen Identifizierung und Quantifizierung von einzelnen oder in Gemischen vorliegenden Biomolekülen und deren Modifikationen
WO2008048345A2 (en) * 2006-02-14 2008-04-24 Perkinelmer Las, Inc. Detection systems for mass labels
CA2645159A1 (en) 2006-03-10 2008-06-26 Michael S. Urdea Multiplex protein fractionation
WO2007127767A2 (en) * 2006-04-28 2007-11-08 Ludwig Institute For Cancer Research Quantification of enzyme activity by mass spectrometry
CN101101283A (zh) * 2006-07-05 2008-01-09 中国科学院大连化学物理研究所 一种分离富集磷酸化肽的方法
WO2008141358A1 (en) * 2007-05-17 2008-11-27 Proteomics International Pty Ltd Fragmentation profile based authentification of protein source
WO2008150763A1 (en) * 2007-05-29 2008-12-11 Waters Investments Limited Apparatus and methods for multidimensional analysis
JP4836875B2 (ja) * 2007-06-13 2011-12-14 株式会社日立ハイテクノロジーズ 修飾ペプチドの分析方法および装置
US8568993B2 (en) * 2007-07-02 2013-10-29 Purdue Research Foundation Detection of glycopeptides and glycoproteins for medical diagnostics
US20100311114A1 (en) * 2007-10-19 2010-12-09 Pronota N.V. Preparation of samples for proteome analysis
CA2722952A1 (en) * 2008-03-31 2009-10-08 Japan Tobacco Inc. Quantitation method of virus
US8628977B2 (en) * 2008-05-02 2014-01-14 Purdue Research Foundation Group specific internal standard technology (GSIST) for simultaneous identification and quantification of small molecules
WO2010109022A1 (en) * 2009-03-27 2010-09-30 Universitetet I Oslo Quantitative proteomics method
US8592216B2 (en) * 2009-04-15 2013-11-26 Wisconsin Alumni Research Foundation Labeling peptides with tertiary amines and other basic functional groups for improved mass spectrometric analysis
US9134318B2 (en) 2009-12-11 2015-09-15 Purdue Research Foundation Detection of oxidized polypeptides
US8383417B2 (en) 2009-12-22 2013-02-26 Thermo Finnigan, Llc Assay for monitoring parathyroid hormone (PTH) variants by tandem mass spectrometry
WO2011109832A2 (en) * 2010-03-05 2011-09-09 The Salk Institute For Biological Studies Methods and compositions for nmr spectroscopic analysis using isotopic labeling schemes
CA2829549A1 (en) * 2010-03-10 2011-09-15 Perfinity Biosciences, Inc. Method for recognition and quantification of multiple analytes in a single analysis
US8455202B2 (en) 2010-03-10 2013-06-04 Perfinity Biosciences, Inc. Affinity selector based recognition and quantification system and method for multiple analytes in a single analysis
US8772043B2 (en) 2010-12-23 2014-07-08 Pioneer Hi Bred International Inc Universal peptide tags for transgene polypeptide analysis by mass spectrometry
JP2014526685A (ja) 2011-09-08 2014-10-06 ザ・リージェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア 代謝流量測定、画像化、および顕微鏡法
WO2013086070A1 (en) 2011-12-07 2013-06-13 Glaxosmithkline Llc Methods for determining total body skeletal muscle mass
US9896715B2 (en) 2011-12-20 2018-02-20 Jcr Pharmaceuticals Co., Ltd. Method for analyzing formyl glycine residue
US9048072B2 (en) * 2012-03-12 2015-06-02 Micromass Uk Limited Method of mass spectrometry and a mass spectrometer
GB201204723D0 (en) * 2012-03-19 2012-05-02 Micromass Ltd Improved time of flight quantitation using alternative characteristic ions
EP2909618B1 (de) * 2012-10-22 2021-02-17 President and Fellows of Harvard College Präzise und interferenzfreie multiplexierte quantitative proteomik mittels massenspektrometrie
WO2014120890A1 (en) 2013-01-31 2014-08-07 Perfinity Biosciences, Inc. Robust, easy to use immobilized enzyme reactors
US9134319B2 (en) 2013-03-15 2015-09-15 The Regents Of The University Of California Method for replacing biomarkers of protein kinetics from tissue samples by biomarkers of protein kinetics from body fluids after isotopic labeling in vivo
JP6079587B2 (ja) * 2013-11-27 2017-02-15 株式会社島津製作所 糖ペプチド解析装置
EP3077115A4 (de) * 2013-12-03 2017-08-09 Perfinity Biosciences, Inc. Einzelreaktor für vereinfachte arbeitsabläufe für die probenvorbereitung
US10018635B2 (en) 2014-07-30 2018-07-10 Novilytic, LLC Method for analyzing samples of a biological fluid
US9786478B2 (en) 2014-12-05 2017-10-10 Purdue Research Foundation Zero voltage mass spectrometry probes and systems
CN107960130A (zh) 2015-02-06 2018-04-24 普度研究基金会 探针、系统、盒及其使用方法
WO2016164530A1 (en) * 2015-04-07 2016-10-13 University Of Maryland, College Park Compositions and methods for high throughput protein sequencing
WO2016196522A1 (en) * 2015-05-29 2016-12-08 Cedars-Sinai Medical Center Correlated peptides for quantitative mass spectrometry
US11085927B2 (en) 2016-06-03 2021-08-10 President And Fellows Of Harvard College Techniques for high throughput targeted proteomic analysis and related systems and methods
JP6835260B2 (ja) * 2017-12-28 2021-02-24 株式会社島津製作所 モノクローナル抗体の簡素化された定量方法
MY204150A (en) * 2019-01-25 2024-08-09 Regeneron Pharma Online chromatography and electrospray ionization mass spectrometer
EP3892989A1 (de) * 2020-04-07 2021-10-13 Ares Trading S.A. Kapillargelelektrophorese und ihre verwendung mit komplexen biologischen molekülen
EP4165085A4 (de) * 2020-06-11 2024-07-31 Rapid Novor, Inc. Reagenzien und verfahren zur antikörpersequenzierung
JP7667684B2 (ja) * 2021-04-06 2025-04-23 株式会社日立製作所 検体の分析前処理方法および検体前処理装置
CN113804897A (zh) * 2021-09-17 2021-12-17 中国食品药品检定研究院 一种冬虫夏草及各发酵虫草制剂特征肽段的序列鉴定方法
US20240036055A1 (en) * 2022-06-15 2024-02-01 Wisconsin Alumni Research Foundation Multiplexed DiLeu-Biotin-Azide (DBA) Tag Enabled Isobaric Tandem Orthogonal Proteolysis Activity-Based Protein Profiling (isoBOP-ABPP) Platform For High-Throughput Quantitative Pan-PTM Analysis
CN117030899A (zh) * 2023-08-28 2023-11-10 中信湘雅生殖与遗传专科医院有限公司 蛋白样品的处理方法及蛋白组分析方法
WO2025057852A1 (ja) * 2023-09-14 2025-03-20 株式会社島津製作所 代謝物の分析前処理方法
CN117347531B (zh) * 2023-12-05 2024-02-13 天津医科大学总医院 一种采用双重内标定量检测蛋白质的方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340966C (en) * 1989-05-19 2000-04-18 Thomas R. Covey Method of protein analysis
US5652128A (en) 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins
US5393669A (en) 1993-02-05 1995-02-28 Martek Biosciences Corp. Compositions and methods for protein structural determinations
IL130949A (en) * 1997-01-23 2004-12-15 Xzillion Gmbh & Co Kg Method for characterising polypeptides
AU771262B2 (en) 1998-08-12 2004-03-18 Eisai Inc. Profiling and cataloging expressed protein tags
US6670194B1 (en) * 1998-08-25 2003-12-30 University Of Washington Rapid quantitative analysis of proteins or protein function in complex mixtures
GB9821669D0 (en) * 1998-10-05 1998-12-02 Glaxo Group Ltd Chemical constructs and their uses
US6629040B1 (en) * 1999-03-19 2003-09-30 University Of Washington Isotope distribution encoded tags for protein identification
US6391649B1 (en) * 1999-05-04 2002-05-21 The Rockefeller University Method for the comparative quantitative analysis of proteins and other biological material by isotopic labeling and mass spectroscopy
EP1311858B1 (de) * 2000-05-05 2006-11-02 Purdue Research Foundation Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen
AU6689401A (en) * 2000-06-12 2001-12-24 Univ Washington Selective labeling and isolation of phosphopeptides and applications to proteomeanalysis
JP4141834B2 (ja) * 2000-10-23 2008-08-27 ジェネティックス インスティテュート エルエルシー マトリックス支援レーザー脱離イオン化マススペクトロメトリーを使用したハイスループットなタンパク質の同定及び定量のための同位体コードイオン化増強試薬(icier)
US20020119490A1 (en) * 2000-12-26 2002-08-29 Aebersold Ruedi H. Methods for rapid and quantitative proteome analysis
US7045296B2 (en) * 2001-05-08 2006-05-16 Applera Corporation Process for analyzing protein samples
US7183116B2 (en) * 2001-05-14 2007-02-27 The Institute For Systems Biology Methods for isolation and labeling of sample molecules
SE0101683D0 (sv) * 2001-05-14 2001-05-14 Torkel Klingberg New method
WO2003027682A2 (en) 2001-09-27 2003-04-03 Purdue Research Foundation Controlling isotope effects during fractionation of analytes
US6970630B2 (en) * 2002-05-23 2005-11-29 Rutgers, The State University Of New Jersey Fiber optic cable and process for manufacturing
US20070269895A1 (en) * 2002-06-03 2007-11-22 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
EP1514107B1 (de) * 2002-06-03 2013-05-15 The Institute for Systems Biology Verfahren zur quantitativen proteomanalyse von glycoproteinen
AU2003259029A1 (en) * 2002-06-04 2003-12-19 The Institute For Systems Biology Methods for high throughput and quantitative proteome analysis
US7473535B2 (en) * 2002-08-20 2009-01-06 The Institute For Systems Biology Chemical reagents and methods for detection and quantification of proteins in complex mixtures
WO2005007677A2 (en) * 2003-07-10 2005-01-27 The Institute For Systems Biology Affinity capture of peptides by microarray and related methods
US20060141528A1 (en) * 2004-05-21 2006-06-29 Aebersold Rudolf H Compositions and methods for quantification of serum glycoproteins
EP1938104A2 (de) * 2005-10-17 2008-07-02 Institute for Systems Biology Von gewebe und serum abgeleitete glycoproteine, und methoden zu deren verwendung

Also Published As

Publication number Publication date
EP1311858A2 (de) 2003-05-21
US6864099B2 (en) 2005-03-08
AU2005209637A1 (en) 2005-09-29
US20080145863A1 (en) 2008-06-19
AU2008213716B2 (en) 2012-03-15
AU2001259470A1 (en) 2001-11-20
CA2402871A1 (en) 2001-11-15
AU2005209637B2 (en) 2008-06-12
US20030092076A1 (en) 2003-05-15
US6872575B2 (en) 2005-03-29
WO2001086306A2 (en) 2001-11-15
JP2003532900A (ja) 2003-11-05
CA2402871C (en) 2011-08-23
IL152620A0 (en) 2003-06-24
AU2008213716A1 (en) 2008-10-02
WO2001086306A3 (en) 2003-03-13
US20030129769A1 (en) 2003-07-10
IL152620A (en) 2010-05-17
DE60124265D1 (de) 2006-12-14
EP1311858B1 (de) 2006-11-02
US20100273178A1 (en) 2010-10-28
US20020037532A1 (en) 2002-03-28
IL200933A (en) 2011-04-28

Similar Documents

Publication Publication Date Title
ATE344456T1 (de) Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen
Orvisky et al. Enrichment of low molecular weight fraction of serum for MS analysis of peptides associated with hepatocellular carcinoma
DE60217328D1 (de) Verfahren zur analyse von proteinproben
JP2003532900A5 (de)
WO2002042427A3 (en) Mass tags for quantitative analysis
DE60029572D1 (de) Isolierung und analyse von proteinen
ATE508361T1 (de) Absolute quantifizierung von proteinen und modifizierten formen davon mittels mehrstufiger massenspektrometrie
WO2004031730A3 (en) High sensitivity quantitation of peptides by mass spectrometry
ATE377756T1 (de) Verfahren und gerät für die identifikation und zuordnung von biomolekülen
WO2001084140A3 (de) Verfahren und vorrichtung zur qualitativen und/oder quantitativen bestimmung eines protein- und/oder peptidmusters einer flüssigkeitsprobe, die dem menschlichen oder tierischen körper entnommen wird
ATE495447T1 (de) Peptidantikörperdepletion und anwendung zur probenvorbereitung für massenspektrometrie
WO2002008767A3 (en) Methods and kits for sequencing polypeptides
WO2004111636A3 (en) Peptide combos and their uses
DE60129239D1 (de) Kit und verfahren zur bestimmung von protein esm-1
DE60215498D1 (de) Verfahren zur bestimmung von polypeptiden
TW200420491A (en) Integrated microfluidic electro-spray chip system and the analysis method thereof
JP2019088213A (ja) コラーゲン由来材料の動物種を判定する方法
DE50105263D1 (de) Verfahren zur analyse von proteinen
DK0960336T3 (da) Peptidreagens til påvisning af humant cytomelagovirus (CMV)
DE60013093D1 (de) Methoden und kits zum sequenzieren von polypeptiden
EP1589341A3 (de) Methode zur selektiven Isolierung von Peptiden zwecks Identifizierung und quantitativer Analyse der Proteine in komplexen Mischungen
WO2003027681A3 (en) A method of protein analysis
WO2000007024A3 (en) Method of protein analysis
WO2008070570A3 (en) Positive selection of serum proteins for proteomic analysis
WO2001084160A3 (en) Proteomic determination of protein nitrotyrosine modifications using mass spectrometry

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties