ATE344456T1 - Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen - Google Patents

Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen

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Publication number
ATE344456T1
ATE344456T1 AT01932999T AT01932999T ATE344456T1 AT E344456 T1 ATE344456 T1 AT E344456T1 AT 01932999 T AT01932999 T AT 01932999T AT 01932999 T AT01932999 T AT 01932999T AT E344456 T1 ATE344456 T1 AT E344456T1
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AT
Austria
Prior art keywords
protein
epptides
affinity
identifying
selected signature
Prior art date
Application number
AT01932999T
Other languages
English (en)
Inventor
Fred E Regnier
Asish B Chakraborty
Xiang Zhang
Original Assignee
Purdue Research Foundation
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Publication of ATE344456T1 publication Critical patent/ATE344456T1/de

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848Methods of protein analysis involving mass spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848Methods of protein analysis involving mass spectrometry
    • G01N33/6851Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/24Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/25Chemistry: analytical and immunological testing including sample preparation

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT01932999T 2000-05-05 2001-05-04 Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen ATE344456T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20322700P 2000-05-05 2000-05-05
US20818400P 2000-05-31 2000-05-31
US20837200P 2000-05-31 2000-05-31

Publications (1)

Publication Number Publication Date
ATE344456T1 true ATE344456T1 (de) 2006-11-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AT01932999T ATE344456T1 (de) 2000-05-05 2001-05-04 Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen

Country Status (9)

Country Link
US (5) US6864099B2 (de)
EP (1) EP1311858B1 (de)
JP (1) JP2003532900A (de)
AT (1) ATE344456T1 (de)
AU (3) AU2001259470A1 (de)
CA (1) CA2402871C (de)
DE (1) DE60124265D1 (de)
IL (2) IL152620A (de)
WO (1) WO2001086306A2 (de)

Families Citing this family (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5910403A (en) * 1997-05-15 1999-06-08 The Regents Of University Of California Methods for measuring cellular proliferation and destruction rates in vitro and in vivo
JP2003532900A (ja) * 2000-05-05 2003-11-05 パーデュー・リサーチ・ファウンデーション タンパク質を同定及び定量化するための親和性により選択されるサインペプチド
WO2002039119A2 (en) * 2000-10-27 2002-05-16 The Johns Hopkins University Method for assaying protein nitrosylation
US20020090652A1 (en) * 2000-12-22 2002-07-11 Fu Emil Wei-Ming Inverse labeling method for the rapid identification of marker/target proteins
US20030077616A1 (en) * 2001-04-19 2003-04-24 Ciphergen Biosystems, Inc. Biomolecule characterization using mass spectrometry and affinity tags
WO2003027682A2 (en) * 2001-09-27 2003-04-03 Purdue Research Foundation Controlling isotope effects during fractionation of analytes
US20030077572A1 (en) * 2001-10-11 2003-04-24 Abramson Fred P. Quantitative differential display using isotope ratio monitoring
IL161498A0 (en) * 2001-10-24 2004-09-27 Univ California Measurement of protein synthesis rates in humans and experimental system by use of isotopically labeled water
WO2003068919A2 (en) * 2002-02-12 2003-08-21 The Regents Of The University Of California Non-invasive method for measuring rates of biosynthesis of biological molecules by label incorporation
AU2003234688A1 (en) * 2002-04-05 2003-10-27 The Regents Of The University Of California Method for isolating and measuring proliferation of long-term label retaining cells and stem cells
DE60328392D1 (de) * 2002-04-30 2009-08-27 Gyros Patent Ab Mittels einer integrierten mikrofluidischen vorrichtung
US20070072179A1 (en) * 2002-05-10 2007-03-29 Frank Essmann Solid-phase assisted spectroscopic and spectrometric analysis of complex biopolymer mixtures
CA2487314A1 (en) * 2002-06-03 2003-12-11 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
US20070269895A1 (en) * 2002-06-03 2007-11-22 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
DE10227599B4 (de) * 2002-06-20 2005-04-28 Proteome Factory Ag Verfahren und Reagenz zur spezifischen Identifizierung und Quantifizierung von einem oder mehreren Proteinen in einer Probe
FR2842826B1 (fr) * 2002-07-25 2006-01-20 Centre Nat Rech Scient Procede de fabrication de puces biologiques
WO2004011426A2 (en) 2002-07-30 2004-02-05 The Regents Of The University Of California Method for automated, large-scale measurement of the molecular flux rates of the proteome or the organeome using mass spectrometry
WO2004015391A2 (en) * 2002-08-12 2004-02-19 Cornell Research Foundation, Inc. Mass spectrometry-based identification of proteins
AU2003268416A1 (en) * 2002-09-04 2004-03-29 The Regents Of The University Of California Methods for measuring rates of replication and death of nfectious microbial agents in an infected host organism
ATE415486T1 (de) * 2002-09-13 2008-12-15 Univ California Verfahren zur messung der geschwindigkeiten des cholesterinrückwärtstransports in vivo als index für anti-artherogenese
US20070248540A1 (en) * 2002-09-16 2007-10-25 The Regents Of The University Of California Biochemical methods for measuring metabolic fitness of tissues or whole organisms
TW200502394A (en) * 2002-09-16 2005-01-16 Univ California Biochemical methods for measuring metabolic fitness of tissues or whole organisms
US7632686B2 (en) 2002-10-03 2009-12-15 Anderson Forschung Group High sensitivity quantitation of peptides by mass spectrometry
CN100558879C (zh) * 2002-10-03 2009-11-11 诺曼·利·安德森 用质谱法高灵敏度定量肽
DE10247896B4 (de) * 2002-10-14 2007-01-18 Bruker Daltonik Gmbh Mehrdimensionale Trennung von Biosubstanzgemischen für massenspektrometrische Analysen
CN1852765A (zh) * 2002-10-18 2006-10-25 普罗梅加公司 用于分离分子的组合物
WO2004038752A2 (en) * 2002-10-21 2004-05-06 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Contiguous capillary electrospray sources and analytical device
AU2003291731B2 (en) 2002-11-04 2009-09-10 The Regents Of The University Of California Deuterated glucose or fat tolerance tests for high-throughput measurement of the metabolism of sugars or fatty acids in the body
US7425451B2 (en) 2002-12-13 2008-09-16 Agilent Technologies, Inc. Triazine derivatives as universal peptide isotope tag reagents (U-PIT)
US7109040B2 (en) * 2002-12-13 2006-09-19 Agilent Technologies, Inc. Modular isotope coding approach to proteomic analysis
US7422865B2 (en) * 2003-01-13 2008-09-09 Agilent Technologies, Inc. Method of identifying peptides in a proteomic sample
JP2004301715A (ja) * 2003-03-31 2004-10-28 Institute Of Physical & Chemical Research タンパク質の一次構造情報収集方法、それに用いる疎水性微細粒子担体及びタンパク質一次構造情報自動取得システム
US7262020B2 (en) * 2003-07-03 2007-08-28 The Regents Of The University Of California Methods for comparing relative flux rates of two or more biological molecules in vivo through a single protocol
AU2004267802B2 (en) * 2003-08-18 2011-03-17 Tethys Bioscience, Inc. Methods for reducing complexity of a sample using small epitope antibodies
US20050202406A1 (en) 2003-11-25 2005-09-15 The Regents Of The University Of California Method for high-throughput screening of compounds and combinations of compounds for discovery and quantification of actions, particularly unanticipated therapeutic or toxic actions, in biological systems
JP2005189104A (ja) 2003-12-25 2005-07-14 Shimadzu Corp タンパク質又はペプチドの濃縮分離法
WO2005078440A2 (en) * 2004-02-06 2005-08-25 Applera Corporation Preparation of biologically derived fluids for biomarker determination by mass spectrometry
WO2005075993A1 (en) * 2004-02-10 2005-08-18 Korea Basic Science Institute Selective labeling agent for phosphoproteome analysis and phosphorylated site analysis
US20070087448A1 (en) * 2004-02-16 2007-04-19 Nelsestuen Gary L Biological profiles and methods of use
TW200538738A (en) 2004-02-20 2005-12-01 Univ California Molecular flux rates through critical pathways measured by stable isotope labeling in vivo, as biomarkers of drug action and disease activity
WO2005087943A1 (en) * 2004-03-11 2005-09-22 The Regents Of The University Of California Temporal or spatial characterization of biosynthetic events in living organisms by isotopic fingerprinting under conditions of imposed isotopic gradients
US20050238577A1 (en) * 2004-03-29 2005-10-27 The Regents Of The University Of California Isolation of epithelial cells or their biochemical contents from excreta after in vivo isotopic labeling
WO2005114700A2 (en) * 2004-05-19 2005-12-01 Applera Corporation Expression quantification using mass spectrometry
US20080206737A1 (en) * 2004-05-19 2008-08-28 Hunter Christie L Expression quantification using mass spectrometry
US20070054345A1 (en) * 2004-05-19 2007-03-08 Hunter Christie L Expression quantification using mass spectrometry
EP1766388A4 (de) * 2004-06-09 2008-08-20 Anderson Forschung Group Llc Mit stabilen isotopen markierte polypeptidstandards zur proteinquantifizierung
KR100611313B1 (ko) * 2004-06-30 2006-08-10 고려대학교 산학협력단 폴리펩티드의 n-말단 치환용 화합물, 이를 이용한폴리펩티드 내의 아미노산 서열분석 및 정량방법
SE0401854D0 (sv) * 2004-07-09 2004-07-09 Amersham Biosciences Ab Method and kit for peptide analysis
WO2006037059A2 (en) * 2004-09-27 2006-04-06 The Johns Hopkins Universtiy Point-of-care mass spectrometer system
US20060251576A1 (en) * 2005-05-03 2006-11-09 The Regents Of The University Of California Methods for measuring cholesterol metabolism and transport
EP1736480B9 (de) * 2005-06-02 2009-03-04 Polyquant GmbH Künstliches Protein, Verfahren für absolute Quantifizierung der Proteine und seine Benutzung
TW200711660A (en) 2005-06-10 2007-04-01 Univ California Monitoring two dimensions of diabetes pathogenesis separately or concurrently (insulin sensitivity and beta-cell sufficiency): uses in diagnosis, prognosis, assessment of disease risk, and drug development
PL1931998T3 (pl) 2005-09-15 2010-11-30 Alk Abello As Sposób ilościowego oznaczania alergenów
WO2007044935A2 (en) * 2005-10-13 2007-04-19 Applera Corporation Methods for the development of a biomolecule assay
EP1785729A3 (de) * 2005-10-17 2007-06-13 Centro De Ingenieria Genetica Y Biotecnologia (Cigb) Verfahren zur selektiven Isolation von in der quantitativen Proteomik anwendbaren mehrfach-geladenen Peptiden
EP1795896A3 (de) * 2005-11-22 2007-06-27 Centro De Ingenieria Genetica Y Biotecnologia (Cigb) Verfahren zur Bestimmung und relativen Quantifizierung von Proteinen basierend auf der selektiven Isolation von PRNK-Peptiden zur Vereinfachung von komplexen Proteinmischungen
CN100465640C (zh) * 2005-11-30 2009-03-04 上海特敏生物医药科技有限公司 一种高灵敏血浆总同型半胱氨酸测定试剂盒
WO2007078229A1 (en) * 2006-01-05 2007-07-12 Ge Healthcare Bio-Sciences Ab Kit and method for mass labelling
DE102006001793A1 (de) * 2006-01-12 2007-07-26 Forschungsgesellschaft für Arbeitsphysiologie und Arbeitsschutz e.V. Verfahren und diagnostisches Kit zur simultanen Identifizierung und Quantifizierung von einzelnen oder in Gemischen vorliegenden Biomolekülen und deren Modifikationen
US20080026480A1 (en) * 2006-02-14 2008-01-31 Perkinelmer Las, Inc. Detection systems for mass labels
US8097425B2 (en) 2006-03-10 2012-01-17 Tethys Bioscience, Inc. Multiplex protein fractionation
EP2021492A2 (de) * 2006-04-28 2009-02-11 Ludwig Institute For Cancer Research Quantifizierung von enzymaktivitäten durch massenspektrometrie
CN101101283A (zh) * 2006-07-05 2008-01-09 中国科学院大连化学物理研究所 一种分离富集磷酸化肽的方法
AU2008253573A1 (en) * 2007-05-17 2008-11-27 Proteomics International Pty Ltd Fragmentation profile based authentification of protein source
WO2008150763A1 (en) * 2007-05-29 2008-12-11 Waters Investments Limited Apparatus and methods for multidimensional analysis
JP4836875B2 (ja) * 2007-06-13 2011-12-14 株式会社日立ハイテクノロジーズ 修飾ペプチドの分析方法および装置
US8568993B2 (en) * 2007-07-02 2013-10-29 Purdue Research Foundation Detection of glycopeptides and glycoproteins for medical diagnostics
WO2009050261A1 (en) * 2007-10-19 2009-04-23 Pronota N.V. Preparation of samples for proteome analysis
KR20100128293A (ko) * 2008-03-31 2010-12-07 니뽄 다바코 산교 가부시키가이샤 바이러스 정량법
WO2009134439A2 (en) * 2008-05-02 2009-11-05 Purdue Research Foundation Group specific internal standard technology (gsist) for simultaneous identification and quantification of small molecules
WO2010109022A1 (en) * 2009-03-27 2010-09-30 Universitetet I Oslo Quantitative proteomics method
US8592216B2 (en) * 2009-04-15 2013-11-26 Wisconsin Alumni Research Foundation Labeling peptides with tertiary amines and other basic functional groups for improved mass spectrometric analysis
WO2011072197A2 (en) 2009-12-11 2011-06-16 Purdue Research Foundation Detection of oxidized polypeptides
US8383417B2 (en) * 2009-12-22 2013-02-26 Thermo Finnigan, Llc Assay for monitoring parathyroid hormone (PTH) variants by tandem mass spectrometry
WO2011109832A2 (en) * 2010-03-05 2011-09-09 The Salk Institute For Biological Studies Methods and compositions for nmr spectroscopic analysis using isotopic labeling schemes
CN103038631A (zh) * 2010-03-10 2013-04-10 珀菲尼迪生物科学股份有限公司 用于在单次分析中识别多种分析物并对其进行定量的方法
US8455202B2 (en) 2010-03-10 2013-06-04 Perfinity Biosciences, Inc. Affinity selector based recognition and quantification system and method for multiple analytes in a single analysis
US8772043B2 (en) 2010-12-23 2014-07-08 Pioneer Hi Bred International Inc Universal peptide tags for transgene polypeptide analysis by mass spectrometry
JP2014526685A (ja) 2011-09-08 2014-10-06 ザ・リージェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア 代謝流量測定、画像化、および顕微鏡法
JP6294233B2 (ja) 2011-12-07 2018-03-14 グラクソスミスクライン エルエルシー 全身骨格筋量の定量方法
WO2013094579A1 (ja) * 2011-12-20 2013-06-27 日本ケミカルリサーチ株式会社 ホルミルグリシン残基の分析法
GB201204723D0 (en) * 2012-03-19 2012-05-02 Micromass Ltd Improved time of flight quantitation using alternative characteristic ions
GB2503538B (en) * 2012-03-27 2015-09-09 Micromass Ltd A method of mass spectrometry and a mass spectrometer
EP2909618B1 (de) * 2012-10-22 2021-02-17 President and Fellows of Harvard College Präzise und interferenzfreie multiplexierte quantitative proteomik mittels massenspektrometrie
WO2014120890A1 (en) 2013-01-31 2014-08-07 Perfinity Biosciences, Inc. Robust, easy to use immobilized enzyme reactors
US9134319B2 (en) 2013-03-15 2015-09-15 The Regents Of The University Of California Method for replacing biomarkers of protein kinetics from tissue samples by biomarkers of protein kinetics from body fluids after isotopic labeling in vivo
JP6079587B2 (ja) * 2013-11-27 2017-02-15 株式会社島津製作所 糖ペプチド解析装置
US10551358B2 (en) * 2013-12-03 2020-02-04 Perfinity Bioscices, Inc. Single reactor for simplified sample preparation workflows
US10018635B2 (en) 2014-07-30 2018-07-10 Novilytic, LLC Method for analyzing samples of a biological fluid
US9786478B2 (en) 2014-12-05 2017-10-10 Purdue Research Foundation Zero voltage mass spectrometry probes and systems
CN113725063B (zh) 2015-02-06 2024-09-20 普度研究基金会 探针、盒、系统和样本分析的方法
WO2016164530A1 (en) * 2015-04-07 2016-10-13 University Of Maryland, College Park Compositions and methods for high throughput protein sequencing
US10352942B2 (en) 2015-05-29 2019-07-16 Cedars-Sinai Medical Center Correlated peptides for quantitative mass spectrometry
WO2017210427A1 (en) 2016-06-03 2017-12-07 President And Fellows Of Harvard College Techniques for high throughput targeted proteomic analysis and related systems and methods
EP3734268B1 (de) * 2017-12-28 2022-07-13 Shimadzu Corporation Vereinfachtes verfahren zur quantifizierung von monoklonalen antikörpern
MX2021008822A (es) * 2019-01-25 2021-09-08 Regeneron Pharma Cromatografia en linea y espectrometro de masas de ionizacion por electrospray.
EP3892989A1 (de) * 2020-04-07 2021-10-13 Ares Trading S.A. Kapillargelelektrophorese und ihre verwendung mit komplexen biologischen molekülen
CA3179958A1 (en) * 2020-06-11 2021-12-16 Thierry Le Bihan Reagents and methods for antibody sequencing
JP7667684B2 (ja) * 2021-04-06 2025-04-23 株式会社日立製作所 検体の分析前処理方法および検体前処理装置
CN113804897A (zh) * 2021-09-17 2021-12-17 中国食品药品检定研究院 一种冬虫夏草及各发酵虫草制剂特征肽段的序列鉴定方法
US20240036055A1 (en) * 2022-06-15 2024-02-01 Wisconsin Alumni Research Foundation Multiplexed DiLeu-Biotin-Azide (DBA) Tag Enabled Isobaric Tandem Orthogonal Proteolysis Activity-Based Protein Profiling (isoBOP-ABPP) Platform For High-Throughput Quantitative Pan-PTM Analysis
CN117030899A (zh) * 2023-08-28 2023-11-10 中信湘雅生殖与遗传专科医院有限公司 蛋白样品的处理方法及蛋白组分析方法
WO2025057852A1 (ja) * 2023-09-14 2025-03-20 株式会社島津製作所 代謝物の分析前処理方法
CN117347531B (zh) * 2023-12-05 2024-02-13 天津医科大学总医院 一种采用双重内标定量检测蛋白质的方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340966C (en) * 1989-05-19 2000-04-18 Thomas R. Covey Method of protein analysis
US5652128A (en) 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins
US5393669A (en) * 1993-02-05 1995-02-28 Martek Biosciences Corp. Compositions and methods for protein structural determinations
DE69817211T2 (de) * 1997-01-23 2004-06-03 Xzillion Gmbh & Co. Kg Charakterisierung von polypeptiden
DE69933679T2 (de) 1998-08-12 2007-08-23 Zycos Inc., Cambridge Darstellung des profils und katalogisierung von exprimierten proteinmarkern
ATE253126T1 (de) * 1998-08-25 2003-11-15 Univ Washington Schnelle quantitative analyse von proteinen oder proteinfunktionen in komplexen gemischen
GB9821669D0 (en) * 1998-10-05 1998-12-02 Glaxo Group Ltd Chemical constructs and their uses
US6629040B1 (en) * 1999-03-19 2003-09-30 University Of Washington Isotope distribution encoded tags for protein identification
US6391649B1 (en) * 1999-05-04 2002-05-21 The Rockefeller University Method for the comparative quantitative analysis of proteins and other biological material by isotopic labeling and mass spectroscopy
JP2003532900A (ja) * 2000-05-05 2003-11-05 パーデュー・リサーチ・ファウンデーション タンパク質を同定及び定量化するための親和性により選択されるサインペプチド
EP1295123A4 (de) * 2000-06-12 2005-02-02 Univ Washington Selektives markieren und isolieren von phosphopeptiden und anwendungen in proteomischen analysen
US6905879B2 (en) 2000-10-23 2005-06-14 Genetics Institute, Inc. Isotope-coded ionization-enhancing reagents (ICIER) for high-throughput protein identification and quantitation using matrix-assisted laser desorption ionization mass spectrometry
US20020119490A1 (en) * 2000-12-26 2002-08-29 Aebersold Ruedi H. Methods for rapid and quantitative proteome analysis
US7045296B2 (en) * 2001-05-08 2006-05-16 Applera Corporation Process for analyzing protein samples
US7183116B2 (en) * 2001-05-14 2007-02-27 The Institute For Systems Biology Methods for isolation and labeling of sample molecules
SE0101683D0 (sv) * 2001-05-14 2001-05-14 Torkel Klingberg New method
WO2003027682A2 (en) * 2001-09-27 2003-04-03 Purdue Research Foundation Controlling isotope effects during fractionation of analytes
US6970630B2 (en) * 2002-05-23 2005-11-29 Rutgers, The State University Of New Jersey Fiber optic cable and process for manufacturing
CA2487314A1 (en) * 2002-06-03 2003-12-11 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
US20070269895A1 (en) * 2002-06-03 2007-11-22 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
AU2003259029A1 (en) * 2002-06-04 2003-12-19 The Institute For Systems Biology Methods for high throughput and quantitative proteome analysis
US7473535B2 (en) * 2002-08-20 2009-01-06 The Institute For Systems Biology Chemical reagents and methods for detection and quantification of proteins in complex mixtures
WO2005007677A2 (en) * 2003-07-10 2005-01-27 The Institute For Systems Biology Affinity capture of peptides by microarray and related methods
DE602005013670D1 (de) * 2004-05-21 2009-05-14 Inst Systems Biology Zusammensetzungen und verfahren zur quantifizierung von serumglykoproteinen
AU2006304605A1 (en) * 2005-10-17 2007-04-26 Institute For Systems Biology Tissue-and serum-derived glycoproteins and methods of their use

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US20080145863A1 (en) 2008-06-19
US20100273178A1 (en) 2010-10-28
WO2001086306A2 (en) 2001-11-15
IL200933A (en) 2011-04-28
US20030092076A1 (en) 2003-05-15
US20020037532A1 (en) 2002-03-28
WO2001086306A3 (en) 2003-03-13
AU2005209637B2 (en) 2008-06-12
AU2005209637A1 (en) 2005-09-29
US6872575B2 (en) 2005-03-29
DE60124265D1 (de) 2006-12-14
IL152620A (en) 2010-05-17
IL152620A0 (en) 2003-06-24
US20030129769A1 (en) 2003-07-10
US6864099B2 (en) 2005-03-08
EP1311858B1 (de) 2006-11-02
CA2402871C (en) 2011-08-23
AU2008213716A1 (en) 2008-10-02
AU2008213716B2 (en) 2012-03-15
CA2402871A1 (en) 2001-11-15
EP1311858A2 (de) 2003-05-21

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