ATE346169T1 - Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen - Google Patents
Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturenInfo
- Publication number
- ATE346169T1 ATE346169T1 AT03255861T AT03255861T ATE346169T1 AT E346169 T1 ATE346169 T1 AT E346169T1 AT 03255861 T AT03255861 T AT 03255861T AT 03255861 T AT03255861 T AT 03255861T AT E346169 T1 ATE346169 T1 AT E346169T1
- Authority
- AT
- Austria
- Prior art keywords
- secondary structure
- sequence
- forming region
- primer
- destruction
- Prior art date
Links
- 238000003556 assay Methods 0.000 title 1
- 230000006378 damage Effects 0.000 title 1
- 230000009977 dual effect Effects 0.000 title 1
- 238000009396 hybridization Methods 0.000 title 1
- 239000002773 nucleotide Substances 0.000 abstract 4
- 125000003729 nucleotide group Chemical group 0.000 abstract 4
- 230000000295 complement effect Effects 0.000 abstract 2
- 108091093088 Amplicon Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000000903 blocking effect Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41226302P | 2002-09-20 | 2002-09-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE346169T1 true ATE346169T1 (de) | 2006-12-15 |
Family
ID=32326255
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03255861T ATE346169T1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen |
| AT06017482T ATE469243T1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06017482T ATE469243T1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8394944B2 (de) |
| EP (2) | EP1785491B1 (de) |
| JP (1) | JP2004113240A (de) |
| AT (2) | ATE346169T1 (de) |
| DE (2) | DE60309817T2 (de) |
| ES (1) | ES2276016T3 (de) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005084277A2 (en) * | 2004-02-27 | 2005-09-15 | University Of Denver | Method of isolating nucleic acid targets |
| SE0401270D0 (sv) * | 2004-05-18 | 2004-05-18 | Fredrik Dahl | Method for amplifying specific nucleic acids in parallel |
| US8632970B2 (en) | 2005-05-09 | 2014-01-21 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
| EP1880206B1 (de) | 2005-05-09 | 2012-11-14 | Affymetrix, Inc. | Multiplex-einfang von nukleinsäuren |
| US7803541B2 (en) | 2005-05-12 | 2010-09-28 | Panomics, Inc. | Multiplex branched-chain DNA assays |
| DK2500439T4 (da) | 2005-06-20 | 2017-11-13 | Advanced Cell Diagnostics Inc | Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer |
| WO2007044427A2 (en) | 2005-10-05 | 2007-04-19 | Panomics, Inc. | Detection of nucleic acids from whole blood |
| WO2007058898A2 (en) | 2005-11-10 | 2007-05-24 | Panomics, Inc. | Detection of nucleic acids through amplification of surrogate nucleic acids |
| US8658361B2 (en) | 2010-10-21 | 2014-02-25 | Advanced Cell Diagnostics, Inc. | Ultra sensitive method for in situ detection of nucleic acids |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| WO2016170179A1 (en) | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on solid support |
| EP3286331B1 (de) * | 2015-04-24 | 2022-12-14 | Qiagen GmbH | Verfahren zur hybridisierung eines nukleinsäuremoleküls |
| US11085073B2 (en) | 2015-04-24 | 2021-08-10 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on a solid support |
| EP3362462B1 (de) | 2015-10-12 | 2021-07-21 | Advanced Cell Diagnostics, Inc. | In-situ-detektion von nukleotidvarianten bei proben mit hohem rauschen sowie zugehörige zusammensetzungen und verfahren |
| EP4556575A3 (de) | 2016-11-21 | 2025-10-08 | Bruker Spatial Biology, Inc. | Verfahren zur sequenzierung von nukleinsäuren |
| US10844430B2 (en) | 2018-01-24 | 2020-11-24 | Qiagen Sciences, Llc | DNA sequencing reaction additive |
| EP3794146B1 (de) | 2018-05-14 | 2025-12-10 | Bruker Spatial Biology, Inc. | Verfahren zur erkennung einer zuvor bestimmten nukleotidesequenz |
| JP7852045B2 (ja) * | 2022-06-20 | 2026-04-27 | 株式会社日立ハイテク | プライマー、ｄｎａ検出方法およびｄｎａ検出キット |
| WO2026080682A1 (en) * | 2024-10-09 | 2026-04-16 | Luminex Corporation | Detection of target nucleic acids using blocker oligonucleotides |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU602875B2 (en) * | 1985-12-18 | 1990-11-01 | British Technology Group Limited | Newcastle disease virus gene clones |
| SE454781B (sv) * | 1986-10-17 | 1988-05-30 | Wallac Oy | Hybridiseringsforfarande for detektion av polynukleotidsekvens |
| US5124246A (en) * | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| US5359100A (en) * | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
| US5030557A (en) * | 1987-11-24 | 1991-07-09 | Ml Technology Venture | Means and method for enhancing nucleic acid hybridization |
| US5219727A (en) | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| GB8920097D0 (en) * | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
| US5200314A (en) * | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| DE69126064T2 (de) * | 1990-06-22 | 1997-08-28 | Hoffmann La Roche | Nachweis von schwachen Metabolisierungsreagentien von Drogen |
| US5849481A (en) * | 1990-07-27 | 1998-12-15 | Chiron Corporation | Nucleic acid hybridization assays employing large comb-type branched polynucleotides |
| DE69207580T2 (de) * | 1992-03-23 | 1996-08-08 | Hoffmann La Roche | Verfahren zum DNS-Nachweis |
| US6187538B1 (en) * | 1993-12-13 | 2001-02-13 | Bayer Corporation | Differential hybridization for relative quantification of variant populations |
| US5827648A (en) * | 1995-12-13 | 1998-10-27 | Chiron Corporation | Differential hybridization for relative quantification of variant populations |
| DE69506393T2 (de) * | 1994-04-04 | 1999-07-01 | Ciba Corning Diagnostics Corp., Medfield, Mass. | Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen |
| US5681702A (en) * | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| US5622822A (en) * | 1994-09-13 | 1997-04-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same |
| US5747248A (en) * | 1994-12-05 | 1998-05-05 | Chiron Corporation | Discontinuous probe design using hybritope mapping |
| US5981180A (en) * | 1995-10-11 | 1999-11-09 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
| ATE238434T1 (de) * | 1995-12-05 | 2003-05-15 | Jorn Erland Koch | Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren |
| US6139800A (en) * | 1997-06-23 | 2000-10-31 | Luminex Corporation | Interlaced lasers for multiple fluorescence measurement |
| US6465175B2 (en) * | 1997-09-04 | 2002-10-15 | Bayer Corporation | Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof |
| US6136568A (en) * | 1997-09-15 | 2000-10-24 | Hiatt; Andrew C. | De novo polynucleotide synthesis using rolling templates |
| US6054568A (en) * | 1998-01-16 | 2000-04-25 | The Perkin-Elmer Corporation | Nucleobase oligomers |
| ATE239801T1 (de) * | 1998-01-22 | 2003-05-15 | Luminex Corp | Mikropartikel mit multiplen fluoreszenz-signalen |
| WO1999037819A2 (en) * | 1998-01-27 | 1999-07-29 | Clinical Micro Sensors, Inc. | Amplification of nucleic acids with electronic detection |
| JP4196236B2 (ja) * | 1998-03-17 | 2008-12-17 | 東洋紡績株式会社 | 核酸増幅用試薬および配列特異的な核酸増幅法 |
| US6046807A (en) * | 1998-05-14 | 2000-04-04 | Luminex Corporation | Diode laser based measurement apparatus |
| US6268147B1 (en) * | 1998-11-02 | 2001-07-31 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
| US6322980B1 (en) * | 1999-04-30 | 2001-11-27 | Aclara Biosciences, Inc. | Single nucleotide detection using degradation of a fluorescent sequence |
| US20020051971A1 (en) | 1999-05-21 | 2002-05-02 | John R. Stuelpnagel | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
| US6287778B1 (en) * | 1999-10-19 | 2001-09-11 | Affymetrix, Inc. | Allele detection using primer extension with sequence-coded identity tags |
| WO2001083826A2 (en) * | 2000-05-03 | 2001-11-08 | Massachusetts Institute Of Technology | Methods and reagents for assembling molecules on solid supports |
| US6214557B1 (en) * | 2000-06-06 | 2001-04-10 | Washington University | Cold sensitive mutant DNA polymerases |
| WO2003002762A2 (en) | 2001-06-29 | 2003-01-09 | Syngenta Participations Ag | Enhanced detection and distinction of differential gene expression by enzymatic probe ligation and amplification |
-
2003
- 2003-09-15 US US10/667,191 patent/US8394944B2/en active Active
- 2003-09-18 ES ES03255861T patent/ES2276016T3/es not_active Expired - Lifetime
- 2003-09-18 AT AT03255861T patent/ATE346169T1/de not_active IP Right Cessation
- 2003-09-18 AT AT06017482T patent/ATE469243T1/de not_active IP Right Cessation
- 2003-09-18 DE DE60309817T patent/DE60309817T2/de not_active Expired - Lifetime
- 2003-09-18 DE DE60332775T patent/DE60332775D1/de not_active Expired - Lifetime
- 2003-09-18 EP EP06017482A patent/EP1785491B1/de not_active Expired - Lifetime
- 2003-09-18 EP EP03255861A patent/EP1428892B1/de not_active Expired - Lifetime
- 2003-09-19 JP JP2003329188A patent/JP2004113240A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US8394944B2 (en) | 2013-03-12 |
| JP2004113240A (ja) | 2004-04-15 |
| DE60309817T2 (de) | 2007-09-13 |
| DE60332775D1 (de) | 2010-07-08 |
| ATE469243T1 (de) | 2010-06-15 |
| EP1785491A1 (de) | 2007-05-16 |
| DE60309817D1 (de) | 2007-01-04 |
| EP1785491B1 (de) | 2010-05-26 |
| US20040132061A1 (en) | 2004-07-08 |
| EP1428892A1 (de) | 2004-06-16 |
| ES2276016T3 (es) | 2007-06-16 |
| EP1428892B1 (de) | 2006-11-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |