ATE346169T1 - Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen - Google Patents
Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturenInfo
- Publication number
- ATE346169T1 ATE346169T1 AT03255861T AT03255861T ATE346169T1 AT E346169 T1 ATE346169 T1 AT E346169T1 AT 03255861 T AT03255861 T AT 03255861T AT 03255861 T AT03255861 T AT 03255861T AT E346169 T1 ATE346169 T1 AT E346169T1
- Authority
- AT
- Austria
- Prior art keywords
- secondary structure
- sequence
- forming region
- primer
- destruction
- Prior art date
Links
- 238000003556 assay Methods 0.000 title 1
- 230000006378 damage Effects 0.000 title 1
- 230000009977 dual effect Effects 0.000 title 1
- 238000009396 hybridization Methods 0.000 title 1
- 239000002773 nucleotide Substances 0.000 abstract 4
- 125000003729 nucleotide group Chemical group 0.000 abstract 4
- 230000000295 complement effect Effects 0.000 abstract 2
- 108091093088 Amplicon Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000000903 blocking effect Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41226302P | 2002-09-20 | 2002-09-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE346169T1 true ATE346169T1 (de) | 2006-12-15 |
Family
ID=32326255
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06017482T ATE469243T1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen |
| AT03255861T ATE346169T1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06017482T ATE469243T1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8394944B2 (de) |
| EP (2) | EP1428892B1 (de) |
| JP (1) | JP2004113240A (de) |
| AT (2) | ATE469243T1 (de) |
| DE (2) | DE60309817T2 (de) |
| ES (1) | ES2276016T3 (de) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005084277A2 (en) * | 2004-02-27 | 2005-09-15 | University Of Denver | Method of isolating nucleic acid targets |
| SE0401270D0 (sv) * | 2004-05-18 | 2004-05-18 | Fredrik Dahl | Method for amplifying specific nucleic acids in parallel |
| EP1880206B1 (de) | 2005-05-09 | 2012-11-14 | Affymetrix, Inc. | Multiplex-einfang von nukleinsäuren |
| US8632970B2 (en) | 2005-05-09 | 2014-01-21 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
| CA2607221A1 (en) | 2005-05-12 | 2006-11-23 | Panomics, Inc. | Multiplex branched-chain dna assays |
| WO2007002006A2 (en) | 2005-06-20 | 2007-01-04 | Panomics, Inc. | Multiplex detection of nucleic acids |
| US7927798B2 (en) | 2005-10-05 | 2011-04-19 | Panomics, Inc. | Detection of nucleic acids from whole blood |
| EP1951905B1 (de) | 2005-11-10 | 2012-04-11 | Affymetrix, Inc. | Nachweis von nukleinsäuren über amplifikation von stellvertreternukleinsäuren |
| CN104849472A (zh) | 2010-10-21 | 2015-08-19 | 领先细胞医疗诊断有限公司 | 用于原位检测核酸的超灵敏方法 |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| WO2016170182A1 (en) | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on a solid support |
| WO2016170179A1 (en) | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on solid support |
| ES2940189T3 (es) * | 2015-04-24 | 2023-05-04 | Miltenyi Biotec Bv & Co Kg | Método para hibridar una molécula de ácido nucleico |
| CN108368543B (zh) | 2015-10-12 | 2023-06-13 | 领先细胞医疗诊断有限公司 | 高噪声样品中原位检测核苷酸变体及相关的组合物和方法 |
| KR102476709B1 (ko) | 2016-11-21 | 2022-12-09 | 나노스트링 테크놀로지스, 인크. | 화학적 조성물 및 이것을 사용하는 방법 |
| US10844430B2 (en) | 2018-01-24 | 2020-11-24 | Qiagen Sciences, Llc | DNA sequencing reaction additive |
| KR102893736B1 (ko) | 2018-05-14 | 2025-11-28 | 브루커 스페이셜 바이올로지, 인크. | 화학 조성물 및 이의 사용 방법 |
| EP4541906A1 (de) * | 2022-06-20 | 2025-04-23 | Hitachi High-Tech Corporation | Primer, dna-nachweisverfahren und dna-nachweiskit |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU602875B2 (en) | 1985-12-18 | 1990-11-01 | British Technology Group Limited | Newcastle disease virus gene clones |
| SE454781B (sv) | 1986-10-17 | 1988-05-30 | Wallac Oy | Hybridiseringsforfarande for detektion av polynukleotidsekvens |
| US5359100A (en) | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
| US5124246A (en) | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| US5030557A (en) * | 1987-11-24 | 1991-07-09 | Ml Technology Venture | Means and method for enhancing nucleic acid hybridization |
| US5219727A (en) | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| GB8920097D0 (en) | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
| US5200314A (en) | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| ES2103756T3 (es) | 1990-06-22 | 1997-10-01 | Hoffmann La Roche | Deteccion de metabolizadores ineficaces de farmacos. |
| US5849481A (en) | 1990-07-27 | 1998-12-15 | Chiron Corporation | Nucleic acid hybridization assays employing large comb-type branched polynucleotides |
| EP0566751B1 (de) * | 1992-03-23 | 1996-01-10 | F. Hoffmann-La Roche Ag | Verfahren zum DNS-Nachweis |
| US5827648A (en) | 1995-12-13 | 1998-10-27 | Chiron Corporation | Differential hybridization for relative quantification of variant populations |
| US6187538B1 (en) | 1993-12-13 | 2001-02-13 | Bayer Corporation | Differential hybridization for relative quantification of variant populations |
| ATE174066T1 (de) | 1994-04-04 | 1998-12-15 | Ciba Corning Diagnostics Corp | Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen |
| US5681702A (en) | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| US5622822A (en) | 1994-09-13 | 1997-04-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same |
| US5747248A (en) | 1994-12-05 | 1998-05-05 | Chiron Corporation | Discontinuous probe design using hybritope mapping |
| US5981180A (en) | 1995-10-11 | 1999-11-09 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
| ES2262902T3 (es) | 1995-12-05 | 2006-12-01 | Jorn Erland Koch | Una reaccion en cascada de amplificacion de acido nucleico. |
| AU8148898A (en) | 1997-06-23 | 1999-01-04 | Luminex Corporation | Interlaced lasers for multiple fluorescence measurement |
| WO1999011813A2 (en) | 1997-09-04 | 1999-03-11 | Bayer Corporation | Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof |
| US6136568A (en) | 1997-09-15 | 2000-10-24 | Hiatt; Andrew C. | De novo polynucleotide synthesis using rolling templates |
| US6054568A (en) * | 1998-01-16 | 2000-04-25 | The Perkin-Elmer Corporation | Nucleobase oligomers |
| DE69907630T2 (de) | 1998-01-22 | 2004-02-26 | Luminex Corp., Austin | Mikropartikel mit multiplen fluoreszenz-signalen |
| EP1051517A2 (de) | 1998-01-27 | 2000-11-15 | Clinical Micro Sensors, Inc. | Amplifizierung von nukleinsäuren mit elektronischer erkennung |
| JP4196236B2 (ja) | 1998-03-17 | 2008-12-17 | 東洋紡績株式会社 | 核酸増幅用試薬および配列特異的な核酸増幅法 |
| JP4471494B2 (ja) | 1998-05-14 | 2010-06-02 | ルミネックス コーポレイション | ダイオードレーザをベースとした測定装置 |
| US6268147B1 (en) * | 1998-11-02 | 2001-07-31 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
| US6322980B1 (en) | 1999-04-30 | 2001-11-27 | Aclara Biosciences, Inc. | Single nucleotide detection using degradation of a fluorescent sequence |
| US20020051971A1 (en) | 1999-05-21 | 2002-05-02 | John R. Stuelpnagel | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
| US6287778B1 (en) | 1999-10-19 | 2001-09-11 | Affymetrix, Inc. | Allele detection using primer extension with sequence-coded identity tags |
| WO2001083826A2 (en) * | 2000-05-03 | 2001-11-08 | Massachusetts Institute Of Technology | Methods and reagents for assembling molecules on solid supports |
| US6214557B1 (en) | 2000-06-06 | 2001-04-10 | Washington University | Cold sensitive mutant DNA polymerases |
| EP1412526A2 (de) | 2001-06-29 | 2004-04-28 | Syngenta Participations AG | Verfahren zur verbesserten detektion und unterschiedung von differenzieller genexpression mittels enzymatischer ligation von proben und amplifizierung |
-
2003
- 2003-09-15 US US10/667,191 patent/US8394944B2/en active Active
- 2003-09-18 AT AT06017482T patent/ATE469243T1/de not_active IP Right Cessation
- 2003-09-18 AT AT03255861T patent/ATE346169T1/de not_active IP Right Cessation
- 2003-09-18 ES ES03255861T patent/ES2276016T3/es not_active Expired - Lifetime
- 2003-09-18 DE DE60309817T patent/DE60309817T2/de not_active Expired - Lifetime
- 2003-09-18 EP EP03255861A patent/EP1428892B1/de not_active Expired - Lifetime
- 2003-09-18 EP EP06017482A patent/EP1785491B1/de not_active Expired - Lifetime
- 2003-09-18 DE DE60332775T patent/DE60332775D1/de not_active Expired - Lifetime
- 2003-09-19 JP JP2003329188A patent/JP2004113240A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP1785491A1 (de) | 2007-05-16 |
| US20040132061A1 (en) | 2004-07-08 |
| EP1428892A1 (de) | 2004-06-16 |
| US8394944B2 (en) | 2013-03-12 |
| ES2276016T3 (es) | 2007-06-16 |
| DE60309817T2 (de) | 2007-09-13 |
| ATE469243T1 (de) | 2010-06-15 |
| DE60309817D1 (de) | 2007-01-04 |
| EP1785491B1 (de) | 2010-05-26 |
| DE60332775D1 (de) | 2010-07-08 |
| EP1428892B1 (de) | 2006-11-22 |
| JP2004113240A (ja) | 2004-04-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |