ATE469243T1 - Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen - Google Patents
Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturenInfo
- Publication number
- ATE469243T1 ATE469243T1 AT06017482T AT06017482T ATE469243T1 AT E469243 T1 ATE469243 T1 AT E469243T1 AT 06017482 T AT06017482 T AT 06017482T AT 06017482 T AT06017482 T AT 06017482T AT E469243 T1 ATE469243 T1 AT E469243T1
- Authority
- AT
- Austria
- Prior art keywords
- secondary structure
- sequence
- forming region
- primer
- destruction
- Prior art date
Links
- 238000003556 assay Methods 0.000 title 1
- 230000006378 damage Effects 0.000 title 1
- 230000009977 dual effect Effects 0.000 title 1
- 238000009396 hybridization Methods 0.000 title 1
- 239000002773 nucleotide Substances 0.000 abstract 4
- 125000003729 nucleotide group Chemical group 0.000 abstract 4
- 230000000295 complement effect Effects 0.000 abstract 2
- 108091093088 Amplicon Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000000903 blocking effect Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41226302P | 2002-09-20 | 2002-09-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE469243T1 true ATE469243T1 (de) | 2010-06-15 |
Family
ID=32326255
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06017482T ATE469243T1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen |
| AT03255861T ATE346169T1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03255861T ATE346169T1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8394944B2 (de) |
| EP (2) | EP1785491B1 (de) |
| JP (1) | JP2004113240A (de) |
| AT (2) | ATE469243T1 (de) |
| DE (2) | DE60309817T2 (de) |
| ES (1) | ES2276016T3 (de) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005084277A2 (en) * | 2004-02-27 | 2005-09-15 | University Of Denver | Method of isolating nucleic acid targets |
| SE0401270D0 (sv) * | 2004-05-18 | 2004-05-18 | Fredrik Dahl | Method for amplifying specific nucleic acids in parallel |
| US8632970B2 (en) | 2005-05-09 | 2014-01-21 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
| EP1880206B1 (de) | 2005-05-09 | 2012-11-14 | Affymetrix, Inc. | Multiplex-einfang von nukleinsäuren |
| ATE502120T1 (de) | 2005-05-12 | 2011-04-15 | Affymetrix Inc | Multiplex-assays für verzweigtkettige dna |
| EP3042963A1 (de) | 2005-06-20 | 2016-07-13 | Advanced Cell Diagnostics, Inc. | Verfahren zum nachweis von nukleinsäuren in einzelnen zellen und zur identifikation seltener zellen aus grossen heterogenen zellpopulationen |
| WO2007044427A2 (en) | 2005-10-05 | 2007-04-19 | Panomics, Inc. | Detection of nucleic acids from whole blood |
| US8017360B2 (en) | 2005-11-10 | 2011-09-13 | Panomics, Inc. | Detection of nucleic acids through amplification of surrogate nucleic acids |
| ES2562821T3 (es) | 2010-10-21 | 2016-03-08 | Advanced Cell Diagnostics, Inc. | Método ultrasensible para la detección in situ de ácidos nucleicos |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| WO2016170182A1 (en) | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on a solid support |
| WO2016170181A1 (en) * | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for hybridizing a nucleic acid molecule |
| US11220705B2 (en) | 2015-04-24 | 2022-01-11 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on solid support |
| CN114350752B (zh) | 2015-07-17 | 2025-02-25 | 布鲁克空间生物学公司 | 在横切面组织的用户定义区域中的基因表达的同时定量 |
| EP3913068B1 (de) | 2015-10-12 | 2023-08-02 | Advanced Cell Diagnostics, Inc. | In-situ-detektion von nukleotidvarianten bei proben mit hohem rauschen sowie zugehörige zusammensetzungen und verfahren |
| AU2017361521B2 (en) | 2016-11-21 | 2020-08-27 | Bruker Spatial Biology, Inc. | Chemical compositions and methods of using same |
| US10844430B2 (en) * | 2018-01-24 | 2020-11-24 | Qiagen Sciences, Llc | DNA sequencing reaction additive |
| KR102893736B1 (ko) | 2018-05-14 | 2025-11-28 | 브루커 스페이셜 바이올로지, 인크. | 화학 조성물 및 이의 사용 방법 |
| CN119173642A (zh) * | 2022-06-20 | 2024-12-20 | 株式会社日立高新技术 | 引物、dna检测方法和dna检测试剂盒 |
| WO2026080682A1 (en) * | 2024-10-09 | 2026-04-16 | Luminex Corporation | Detection of target nucleic acids using blocker oligonucleotides |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU602875B2 (en) * | 1985-12-18 | 1990-11-01 | British Technology Group Limited | Newcastle disease virus gene clones |
| SE454781B (sv) * | 1986-10-17 | 1988-05-30 | Wallac Oy | Hybridiseringsforfarande for detektion av polynukleotidsekvens |
| US5359100A (en) * | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
| US5124246A (en) * | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| US5030557A (en) * | 1987-11-24 | 1991-07-09 | Ml Technology Venture | Means and method for enhancing nucleic acid hybridization |
| US5219727A (en) | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| GB8920097D0 (en) * | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
| US5200314A (en) * | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| DE69126064T2 (de) * | 1990-06-22 | 1997-08-28 | Hoffmann La Roche | Nachweis von schwachen Metabolisierungsreagentien von Drogen |
| US5849481A (en) * | 1990-07-27 | 1998-12-15 | Chiron Corporation | Nucleic acid hybridization assays employing large comb-type branched polynucleotides |
| ES2082256T3 (es) * | 1992-03-23 | 1996-03-16 | Hoffmann La Roche | Metodo de deteccion del adn. |
| US5827648A (en) * | 1995-12-13 | 1998-10-27 | Chiron Corporation | Differential hybridization for relative quantification of variant populations |
| US6187538B1 (en) * | 1993-12-13 | 2001-02-13 | Bayer Corporation | Differential hybridization for relative quantification of variant populations |
| BR9507343A (pt) * | 1994-04-04 | 1997-09-16 | Ciba Corning Diagnostics Corp | Testes de hibridização - ligação para a detecção de sequências de ácido nucléico específicas |
| US5681702A (en) * | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| US5622822A (en) * | 1994-09-13 | 1997-04-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same |
| US5747248A (en) * | 1994-12-05 | 1998-05-05 | Chiron Corporation | Discontinuous probe design using hybritope mapping |
| US5981180A (en) * | 1995-10-11 | 1999-11-09 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
| DE69636080T2 (de) * | 1995-12-05 | 2006-11-30 | Erland Jorn Koch | Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren |
| AU8148898A (en) * | 1997-06-23 | 1999-01-04 | Luminex Corporation | Interlaced lasers for multiple fluorescence measurement |
| AU9220498A (en) * | 1997-09-04 | 1999-03-22 | Bayer Corporation | Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof |
| US6136568A (en) * | 1997-09-15 | 2000-10-24 | Hiatt; Andrew C. | De novo polynucleotide synthesis using rolling templates |
| US6054568A (en) * | 1998-01-16 | 2000-04-25 | The Perkin-Elmer Corporation | Nucleobase oligomers |
| CA2318779C (en) * | 1998-01-22 | 2009-08-25 | Luminex Corporation | Microparticles with multiple fluorescent signals |
| EP1051517A2 (de) * | 1998-01-27 | 2000-11-15 | Clinical Micro Sensors, Inc. | Amplifizierung von nukleinsäuren mit elektronischer erkennung |
| JP4196236B2 (ja) * | 1998-03-17 | 2008-12-17 | 東洋紡績株式会社 | 核酸増幅用試薬および配列特異的な核酸増幅法 |
| WO1999058958A1 (en) * | 1998-05-14 | 1999-11-18 | Luminex Corporation | Diode laser based measurement apparatus |
| US6268147B1 (en) * | 1998-11-02 | 2001-07-31 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
| US6322980B1 (en) * | 1999-04-30 | 2001-11-27 | Aclara Biosciences, Inc. | Single nucleotide detection using degradation of a fluorescent sequence |
| US20020051971A1 (en) | 1999-05-21 | 2002-05-02 | John R. Stuelpnagel | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
| US6287778B1 (en) * | 1999-10-19 | 2001-09-11 | Affymetrix, Inc. | Allele detection using primer extension with sequence-coded identity tags |
| US20020028455A1 (en) * | 2000-05-03 | 2002-03-07 | Laibinis Paul E. | Methods and reagents for assembling molecules on solid supports |
| US6214557B1 (en) * | 2000-06-06 | 2001-04-10 | Washington University | Cold sensitive mutant DNA polymerases |
| WO2003002762A2 (en) | 2001-06-29 | 2003-01-09 | Syngenta Participations Ag | Enhanced detection and distinction of differential gene expression by enzymatic probe ligation and amplification |
-
2003
- 2003-09-15 US US10/667,191 patent/US8394944B2/en active Active
- 2003-09-18 DE DE60309817T patent/DE60309817T2/de not_active Expired - Lifetime
- 2003-09-18 AT AT06017482T patent/ATE469243T1/de not_active IP Right Cessation
- 2003-09-18 EP EP06017482A patent/EP1785491B1/de not_active Expired - Lifetime
- 2003-09-18 EP EP03255861A patent/EP1428892B1/de not_active Expired - Lifetime
- 2003-09-18 AT AT03255861T patent/ATE346169T1/de not_active IP Right Cessation
- 2003-09-18 DE DE60332775T patent/DE60332775D1/de not_active Expired - Lifetime
- 2003-09-18 ES ES03255861T patent/ES2276016T3/es not_active Expired - Lifetime
- 2003-09-19 JP JP2003329188A patent/JP2004113240A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP1785491A1 (de) | 2007-05-16 |
| ES2276016T3 (es) | 2007-06-16 |
| ATE346169T1 (de) | 2006-12-15 |
| EP1428892B1 (de) | 2006-11-22 |
| DE60309817D1 (de) | 2007-01-04 |
| JP2004113240A (ja) | 2004-04-15 |
| US8394944B2 (en) | 2013-03-12 |
| US20040132061A1 (en) | 2004-07-08 |
| DE60309817T2 (de) | 2007-09-13 |
| EP1428892A1 (de) | 2004-06-16 |
| DE60332775D1 (de) | 2010-07-08 |
| EP1785491B1 (de) | 2010-05-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |