ATE350485T1 - Verfahren zum nachweis von acetylase oder deacetylase aktivität in einem immunoassay - Google Patents

Verfahren zum nachweis von acetylase oder deacetylase aktivität in einem immunoassay

Info

Publication number
ATE350485T1
ATE350485T1 AT01974181T AT01974181T ATE350485T1 AT E350485 T1 ATE350485 T1 AT E350485T1 AT 01974181 T AT01974181 T AT 01974181T AT 01974181 T AT01974181 T AT 01974181T AT E350485 T1 ATE350485 T1 AT E350485T1
Authority
AT
Austria
Prior art keywords
modified
acetylase
immunoassay
peptide
antibody
Prior art date
Application number
AT01974181T
Other languages
English (en)
Inventor
Joachim Kraemer
Christine Peiker
Karsten Henco
Original Assignee
Evotec Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP00117457A external-priority patent/EP1182263A1/de
Priority claimed from EP00122707A external-priority patent/EP1199370B1/de
Priority claimed from EP00128176A external-priority patent/EP1217078A1/de
Application filed by Evotec Ag filed Critical Evotec Ag
Application granted granted Critical
Publication of ATE350485T1 publication Critical patent/ATE350485T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/081—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing O or S as heteroatoms, e.g. Cys, Ser
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0812—Tripeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1005—Tetrapeptides with the first amino acid being neutral and aliphatic
    • C07K5/1013—Tetrapeptides with the first amino acid being neutral and aliphatic the side chain containing O or S as heteroatoms, e.g. Cys, Ser
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1016—Tetrapeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91045—Acyltransferases (2.3)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Enzymes And Modification Thereof (AREA)
AT01974181T 2000-08-11 2001-08-13 Verfahren zum nachweis von acetylase oder deacetylase aktivität in einem immunoassay ATE350485T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP00117457A EP1182263A1 (de) 2000-08-11 2000-08-11 Verfahren zum Nachweis einer Threonin- oder Serinkinasenaktivität
EP00118431 2000-08-24
EP00122707A EP1199370B1 (de) 2000-10-18 2000-10-18 Verfahren zur Auffindung von Dephosphorylierung phosphorylierte Threonin oder Serin durch Phosphataseaktivität
EP00128176A EP1217078A1 (de) 2000-12-21 2000-12-21 Phosphatase-Immunoassay mit Antikörper gegen bisphosphorylierte Substrate

Publications (1)

Publication Number Publication Date
ATE350485T1 true ATE350485T1 (de) 2007-01-15

Family

ID=27439967

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01974181T ATE350485T1 (de) 2000-08-11 2001-08-13 Verfahren zum nachweis von acetylase oder deacetylase aktivität in einem immunoassay

Country Status (5)

Country Link
EP (1) EP1313876B8 (de)
AT (1) ATE350485T1 (de)
AU (1) AU2001293768A1 (de)
DE (1) DE60125783T2 (de)
WO (1) WO2002014543A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005030947A2 (en) 2003-09-30 2005-04-07 Evotec Neurosciences Gmbh Diagnostic and therapeutic use of a sulfotransferase for neurodegenerative diseases
ATE544071T1 (de) * 2005-03-03 2012-02-15 Sirtris Pharmaceuticals Inc Fluoreszenzpolarisationstests für die acetyltransferase-deacetylase-aktivität
WO2006128902A1 (en) 2005-06-01 2006-12-07 Evotec Neurosciences Gmbh Diagnostic and therapeutic target slc39a12 proteins for neurodegenerative diseases
DE602006019582D1 (de) 2005-06-16 2011-02-24 Evotec Neurosciences Gmbh Adarb2 proteine als diagnostische und therapeutische targets für neurodegenerative erkrankungen
EP1957979A1 (de) * 2005-11-25 2008-08-20 Koninklijke Philips Electronics N.V. Magnetischer biosensor zur bestimmung einer enzymaktivität
WO2007064902A2 (en) * 2005-12-02 2007-06-07 Sirtris Pharmaceuticals, Inc. Mass spectrometry assays for acetyltransferase/deacetylase activity
EP2053397A1 (de) 2007-10-25 2009-04-29 EVOTEC Neurosciences GmbH PPM1E-Proteine und Nukleinsäuren als Targets für neurodegenerative Erkrankungen

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE233325T1 (de) * 1997-12-05 2003-03-15 Upjohn Co Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase

Also Published As

Publication number Publication date
EP1313876A2 (de) 2003-05-28
EP1313876B1 (de) 2007-01-03
EP1313876B8 (de) 2007-02-28
WO2002014543A3 (en) 2003-03-20
DE60125783D1 (de) 2007-02-15
AU2001293768A1 (en) 2002-02-25
DE60125783T2 (de) 2007-10-18
WO2002014543A2 (en) 2002-02-21

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