ATE350485T1 - Verfahren zum nachweis von acetylase oder deacetylase aktivität in einem immunoassay - Google Patents

Verfahren zum nachweis von acetylase oder deacetylase aktivität in einem immunoassay

Info

Publication number
ATE350485T1
ATE350485T1 AT01974181T AT01974181T ATE350485T1 AT E350485 T1 ATE350485 T1 AT E350485T1 AT 01974181 T AT01974181 T AT 01974181T AT 01974181 T AT01974181 T AT 01974181T AT E350485 T1 ATE350485 T1 AT E350485T1
Authority
AT
Austria
Prior art keywords
modified
acetylase
immunoassay
peptide
antibody
Prior art date
Application number
AT01974181T
Other languages
English (en)
Inventor
Joachim Kraemer
Christine Peiker
Karsten Henco
Original Assignee
Evotec Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP00117457A external-priority patent/EP1182263A1/de
Priority claimed from EP00122707A external-priority patent/EP1199370B1/de
Priority claimed from EP00128176A external-priority patent/EP1217078A1/de
Application filed by Evotec Ag filed Critical Evotec Ag
Application granted granted Critical
Publication of ATE350485T1 publication Critical patent/ATE350485T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/081—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing O or S as heteroatoms, e.g. Cys, Ser
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0812—Tripeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1005—Tetrapeptides with the first amino acid being neutral and aliphatic
    • C07K5/1013—Tetrapeptides with the first amino acid being neutral and aliphatic the side chain containing O or S as heteroatoms, e.g. Cys, Ser
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1016—Tetrapeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91045—Acyltransferases (2.3)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Enzymes And Modification Thereof (AREA)
AT01974181T 2000-08-11 2001-08-13 Verfahren zum nachweis von acetylase oder deacetylase aktivität in einem immunoassay ATE350485T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP00117457A EP1182263A1 (de) 2000-08-11 2000-08-11 Verfahren zum Nachweis einer Threonin- oder Serinkinasenaktivität
EP00118431 2000-08-24
EP00122707A EP1199370B1 (de) 2000-10-18 2000-10-18 Verfahren zur Auffindung von Dephosphorylierung phosphorylierte Threonin oder Serin durch Phosphataseaktivität
EP00128176A EP1217078A1 (de) 2000-12-21 2000-12-21 Phosphatase-Immunoassay mit Antikörper gegen bisphosphorylierte Substrate

Publications (1)

Publication Number Publication Date
ATE350485T1 true ATE350485T1 (de) 2007-01-15

Family

ID=27439967

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01974181T ATE350485T1 (de) 2000-08-11 2001-08-13 Verfahren zum nachweis von acetylase oder deacetylase aktivität in einem immunoassay

Country Status (5)

Country Link
EP (1) EP1313876B8 (de)
AT (1) ATE350485T1 (de)
AU (1) AU2001293768A1 (de)
DE (1) DE60125783T2 (de)
WO (1) WO2002014543A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1670943B1 (de) 2003-09-30 2013-11-13 Evotec International GmbH Diagnostische und therapeutische verwendung einer sulfotransferase für alzheimer-krankheit
ATE544071T1 (de) * 2005-03-03 2012-02-15 Sirtris Pharmaceuticals Inc Fluoreszenzpolarisationstests für die acetyltransferase-deacetylase-aktivität
EP1891234B1 (de) 2005-06-01 2014-12-10 Evotec International GmbH Verwendung von slc39a12-proteinen als zielmoleküle in der diagnose von und beim screening von medikamenten zur behandlung von alzheimer-krankheit
WO2006134128A2 (en) 2005-06-16 2006-12-21 Evotec Neurosciences Gmbh Diagnostic and therapeutic target adarb2 proteins for neurodegenerative diseases
US20080311598A1 (en) * 2005-11-25 2008-12-18 Koninklijke Philips Electronics, N.V. Magnetic Biosensor For Determination of Enzymic Activity
EP1955077B1 (de) * 2005-12-02 2012-06-13 Sirtris Pharmaceuticals, Inc. Massen-spektronomie-tests für die acetyltransferase-deacetylase-aktivität
EP2053397A1 (de) 2007-10-25 2009-04-29 EVOTEC Neurosciences GmbH PPM1E-Proteine und Nukleinsäuren als Targets für neurodegenerative Erkrankungen
AU2018388549B2 (en) * 2017-12-18 2023-06-01 Yale University Compounds and compositions for treating fibrosis

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1036192T3 (da) * 1997-12-05 2003-06-10 Upjohn Co Fluorescensbaserede screeningsanalyser med høj hastighedforproteinkinaser og phosphataser

Also Published As

Publication number Publication date
DE60125783D1 (de) 2007-02-15
EP1313876B1 (de) 2007-01-03
EP1313876A2 (de) 2003-05-28
WO2002014543A3 (en) 2003-03-20
AU2001293768A1 (en) 2002-02-25
EP1313876B8 (de) 2007-02-28
WO2002014543A2 (en) 2002-02-21
DE60125783T2 (de) 2007-10-18

Similar Documents

Publication Publication Date Title
Emmerich et al. Optimising methods for the preservation, capture and identification of ubiquitin chains and ubiquitylated proteins by immunoblotting
Knisatschek et al. Characterization of “thyroliberin-deamidating enzyme” as a post-proline-cleaving enzyme. Partial purification and enzyme-chemical analysis of the enzyme from anterior pituitary tissue.
ATE279203T1 (de) Verwendung von substrat-subtraktionsbibliotheken zur unterscheidung von enzymspezifitäten
EA200500395A1 (ru) Способы и композиции обнаружения и анализа полинуклеотидов с помощью светособирающих полихромофоров
DE69028639D1 (de) Enzymverstärkte lanthanidchelat-lumineszenzbestimmung
DE602004021281D1 (de) Kolorimetrische substrate, kolorimetrische sensoren und verwendungsverfahren
WO2003036256A3 (en) Method and apparatus for increasing the dynamic range and accuracy of binding assays
AU3625999A (en) Detection of a target in a sample
WO2002088376A3 (en) Multiple simultaneous antigen detection by immunohistochemistry
DE60125783D1 (de) Verfahren zum nachweis von acetylase oder deacetylase aktivität in einem immunoassay
WO2003021226A3 (en) Peptide biomarker and method of identification
WO2003100413A3 (en) Surface acoustic wave sensors and methods for detecting target analytes
CY1107621T1 (el) Μεθοδος ανιχνευσης αντισωματων σε ενα δειγμα
Korecká et al. Utilization of newly developed immobilized enzyme reactors for preparation and study of immunoglobulin G fragments
EP1318401A3 (de) Verfahren zur Früherkennung der Serokonversion eines gegen Hepatitis C-Virus gerichteten Antikörpers
JP2005538380A5 (de)
WO2003012393A3 (en) Method of detecting protease activity in a cell
DE60038363D1 (de) Detektion von kleinen molekülen mit piezo-elektrischem sensor
DE60030291D1 (de) Fluoreszenzintenstitätsmethode zur bestimmung der bindung zwischen proteinen oder peptiden
ATE306666T1 (de) Verfahren zur eiweissanalyse
US7875436B2 (en) Peptide substrates recognizable by a botulinum toxin A, BoNT/A and the use thereof
Norman et al. Proteolytic conversion of [Met] enkephalin-Arg6-Gly7-Leu8 by brain synaptic membranes. Characterization of formed peptides and mechanism of proteolysis.
Barros et al. Neprilysin carboxydipeptidase specificity studies and improvement in its detection with fluorescence energy transfer peptides.
JP2003535103A (ja) ＢｏＮＴ／Ｂ型のボツリヌス毒素により認識されるペプチド基質並びに前記毒素もしくは対応の阻害物質をアッセイ及び／もしくは検出する為のそれらの利用
DE60004840D1 (de) Allergennachweis

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties