ATE544071T1 - Fluoreszenzpolarisationstests für die acetyltransferase-deacetylase-aktivität - Google Patents
Fluoreszenzpolarisationstests für die acetyltransferase-deacetylase-aktivitätInfo
- Publication number
- ATE544071T1 ATE544071T1 AT06736983T AT06736983T ATE544071T1 AT E544071 T1 ATE544071 T1 AT E544071T1 AT 06736983 T AT06736983 T AT 06736983T AT 06736983 T AT06736983 T AT 06736983T AT E544071 T1 ATE544071 T1 AT E544071T1
- Authority
- AT
- Austria
- Prior art keywords
- acetyltransferase
- activity
- fluorescence polarization
- methods
- deacetylase activity
- Prior art date
Links
- 238000002875 fluorescence polarization Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 abstract 4
- 102000005421 acetyltransferase Human genes 0.000 abstract 3
- 108020002494 acetyltransferase Proteins 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 239000000758 substrate Substances 0.000 abstract 3
- 230000021736 acetylation Effects 0.000 abstract 2
- 238000006640 acetylation reaction Methods 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/91045—Acyltransferases (2.3)
- G01N2333/91051—Acyltransferases other than aminoacyltransferases (general) (2.3.1)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/978—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- G01N2333/98—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US65871105P | 2005-03-03 | 2005-03-03 | |
| US70560905P | 2005-08-04 | 2005-08-04 | |
| US74178305P | 2005-12-02 | 2005-12-02 | |
| PCT/US2006/007748 WO2006094239A2 (en) | 2005-03-03 | 2006-03-03 | Fluorescence polarization assays for acetyltransferase/deacetylase activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE544071T1 true ATE544071T1 (de) | 2012-02-15 |
Family
ID=36685597
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06736983T ATE544071T1 (de) | 2005-03-03 | 2006-03-03 | Fluoreszenzpolarisationstests für die acetyltransferase-deacetylase-aktivität |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20080293081A1 (de) |
| EP (1) | EP1853920B1 (de) |
| AT (1) | ATE544071T1 (de) |
| AU (1) | AU2006218407B2 (de) |
| CA (1) | CA2600154A1 (de) |
| ES (1) | ES2380679T3 (de) |
| WO (1) | WO2006094239A2 (de) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2389111T3 (es) | 2005-12-02 | 2012-10-23 | Sirtris Pharmaceuticals, Inc. | Ensayos de espectrometría de masas para la actividad acetiltransferasa/desacetilasa |
| JP2008184403A (ja) * | 2007-01-29 | 2008-08-14 | Japan Health Science Foundation | 新規ｃ型肝炎ウイルス阻害剤 |
| EA021938B1 (ru) | 2008-05-01 | 2015-10-30 | Сертрис Фармасьютикалз, Инк. | Хинолины и их аналоги в качестве модуляторов сиртуина |
| KR20110036602A (ko) | 2008-07-03 | 2011-04-07 | 서트리스 파마슈티컬즈, 인코포레이티드 | 시르투인 조절제로서의 벤즈이미다졸 및 관련 유사체 |
| AU2009295948B2 (en) | 2008-09-29 | 2013-12-05 | GlaxoSmithKline, LLC | Quinazolinone, quinolone and related analogs as sirtuin modulators |
| US9556201B2 (en) | 2009-10-29 | 2017-01-31 | Glaxosmithkline Llc | Bicyclic pyridines and analogs as sirtuin modulators |
| WO2011133559A2 (en) * | 2010-04-19 | 2011-10-27 | University Of Georgia Research Foundation, Inc. | Alpha tubulin acetyltransferase |
| US9932621B2 (en) | 2010-07-07 | 2018-04-03 | Cornell University | Modulators for Sirt5 and assays for screening same |
| WO2013067391A1 (en) * | 2011-11-02 | 2013-05-10 | The Broad Institute, Inc. | Fluorescent substrates for determining lysine modifying enzyme activity |
| US8916528B2 (en) | 2011-11-16 | 2014-12-23 | Resveratrol Partners, Llc | Compositions containing resveratrol and nucleotides |
| US9745613B2 (en) | 2014-07-22 | 2017-08-29 | The Broad Institute, Inc. | Compounds, substrates and methods related to histone deacetylases |
| WO2020219598A1 (en) * | 2019-04-22 | 2020-10-29 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for modulation of acetyltransferase activity and applications thereof including treatments |
| CN112920115A (zh) * | 2019-12-05 | 2021-06-08 | 四川大学 | 喹啉-4-甲酰胺类骨架衍生物及其用途 |
| CN113248528B (zh) * | 2020-02-07 | 2022-05-31 | 天津师范大学 | 基于六氟硅酸银合成的三维Ag(I)配合物及其合成方法与应用 |
| CN113416181B (zh) * | 2021-08-02 | 2022-05-03 | 四川大学 | 喹唑啉类衍生物及其用途 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001149081A (ja) * | 1999-11-29 | 2001-06-05 | Cyclex Co Ltd | 脱アセチル化酵素の活性測定方法、並びにこれら酵素の阻害剤もしくは促進剤のスクリーニング方法 |
| US7452664B2 (en) * | 1999-12-15 | 2008-11-18 | Massachusetts Institute Of Technology | Methods for identifying agents which alter histone protein acetylation |
| EP1156114A1 (de) * | 2000-05-10 | 2001-11-21 | Europäisches Laboratorium für Molekularbiologie | Vektoren für transposon-gestützte DNA Sequenzierungsverfahren |
| AU2001272238A1 (en) * | 2000-06-22 | 2002-01-02 | Mcgill University | CLK-2, CEX-7 and COQ-4 genes, and uses thereof |
| DE60125783T2 (de) * | 2000-08-11 | 2007-10-18 | Evotec Ag | Verfahren zum nachweis von acetylase oder deacetylase-aktivität in einem immunoassay |
| US20040005574A1 (en) * | 2002-07-08 | 2004-01-08 | Leonard Guarente | SIR2 activity |
| AU2002356992A1 (en) * | 2001-11-21 | 2003-06-10 | Albert Einstein College Of Medicine Of Yeshiva University | Sir2 products and activities |
| US7351542B2 (en) * | 2002-05-20 | 2008-04-01 | The Regents Of The University Of California | Methods of modulating tubulin deacetylase activity |
| US20030224473A1 (en) * | 2002-05-23 | 2003-12-04 | Mccafferty Dewey G. | Methods for screening for histone deacetylase activity and for identifying histone deacetylase inhibitors |
| US20030224469A1 (en) * | 2002-06-03 | 2003-12-04 | Buchholz Tonia J. | Methods and kits for assays utilizing fluorescence polarization |
| AU2003256701A1 (en) * | 2002-07-24 | 2004-02-09 | Schering Corporation | Methods of modulating proliferative conditions |
| MXPA05009245A (es) * | 2003-03-11 | 2005-10-19 | Pfizer Prod Inc | Nuevos compuestos de pirazina como inhibidores del factor de crecimiento transformante (tgf). |
| US20050096256A1 (en) * | 2003-07-01 | 2005-05-05 | President And Fellows Of Harvard College | Compositions for manipulating the lifespan and stress response of cells and organisms |
| WO2005002527A2 (en) * | 2003-07-03 | 2005-01-13 | Massachusetts Institute Of Technology | Sirt1 modulation of adipogenesis and adipose function |
| WO2005062952A2 (en) * | 2003-12-23 | 2005-07-14 | The Regents Of The University Of California | Compositions and methods for modulating sirtuin activity |
| US20060014705A1 (en) * | 2004-06-30 | 2006-01-19 | Howitz Konrad T | Compositions and methods for selectively activating human sirtuins |
| US7998974B2 (en) * | 2005-03-03 | 2011-08-16 | Sirtris Pharmaceuticals, Inc. | Fused heterocyclic compounds and their use as sirtuin modulators |
| JP2008535790A (ja) * | 2005-03-03 | 2008-09-04 | サートリス ファーマシューティカルズ， インコーポレイテッド | サーチュインモジュレーターであるｎ−フェニルベンズアミド誘導体 |
| US20070099830A1 (en) * | 2005-04-21 | 2007-05-03 | Massachusetts Institute Of Technology | Sirt4 activities |
| ES2389111T3 (es) * | 2005-12-02 | 2012-10-23 | Sirtris Pharmaceuticals, Inc. | Ensayos de espectrometría de masas para la actividad acetiltransferasa/desacetilasa |
| US8043804B2 (en) * | 2007-05-23 | 2011-10-25 | The Trustees Of Columbia University In The City Of New York | DBC1, a novel native inhibitor of anti-aging protein SIRT1 |
| US20100168084A1 (en) * | 2008-05-08 | 2010-07-01 | Huber L Julie | Therapeutic compounds and related methods of use |
-
2006
- 2006-03-03 WO PCT/US2006/007748 patent/WO2006094239A2/en not_active Ceased
- 2006-03-03 AT AT06736983T patent/ATE544071T1/de active
- 2006-03-03 ES ES06736983T patent/ES2380679T3/es not_active Expired - Lifetime
- 2006-03-03 EP EP06736983A patent/EP1853920B1/de not_active Expired - Lifetime
- 2006-03-03 CA CA002600154A patent/CA2600154A1/en not_active Abandoned
- 2006-03-03 AU AU2006218407A patent/AU2006218407B2/en not_active Ceased
- 2006-03-03 US US11/885,390 patent/US20080293081A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20080293081A1 (en) | 2008-11-27 |
| CA2600154A1 (en) | 2006-09-08 |
| WO2006094239A3 (en) | 2007-03-29 |
| AU2006218407B2 (en) | 2012-11-15 |
| WO2006094239A2 (en) | 2006-09-08 |
| EP1853920A2 (de) | 2007-11-14 |
| ES2380679T3 (es) | 2012-05-17 |
| EP1853920B1 (de) | 2012-02-01 |
| AU2006218407A1 (en) | 2006-09-08 |
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