ATE351912T1 - Signalpeptide aus lactococcus lactis - Google Patents

Signalpeptide aus lactococcus lactis

Info

Publication number
ATE351912T1
ATE351912T1 AT00951271T AT00951271T ATE351912T1 AT E351912 T1 ATE351912 T1 AT E351912T1 AT 00951271 T AT00951271 T AT 00951271T AT 00951271 T AT00951271 T AT 00951271T AT E351912 T1 ATE351912 T1 AT E351912T1
Authority
AT
Austria
Prior art keywords
signal peptides
molecule
reporter gene
dna molecule
reporter
Prior art date
Application number
AT00951271T
Other languages
English (en)
Inventor
Peter Ravn
Soeren Michael Madsen
Astrid Vrang
Hans Israelsen
Mads Groenvold Johnsen
Lars Bredmose
Jose Arnau
Original Assignee
Bioneer As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioneer As filed Critical Bioneer As
Application granted granted Critical
Publication of ATE351912T1 publication Critical patent/ATE351912T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT00951271T 1999-08-06 2000-08-04 Signalpeptide aus lactococcus lactis ATE351912T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DKPA199901105 1999-08-06

Publications (1)

Publication Number Publication Date
ATE351912T1 true ATE351912T1 (de) 2007-02-15

Family

ID=8101062

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00951271T ATE351912T1 (de) 1999-08-06 2000-08-04 Signalpeptide aus lactococcus lactis

Country Status (11)

Country Link
US (1) US7186815B2 (de)
EP (1) EP1206556B1 (de)
JP (1) JP4271396B2 (de)
AT (1) ATE351912T1 (de)
AU (1) AU772650B2 (de)
CA (1) CA2381184C (de)
DE (1) DE60033037T2 (de)
DK (1) DK1206556T3 (de)
ES (1) ES2276691T3 (de)
NZ (1) NZ517079A (de)
WO (1) WO2001011060A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4507319B2 (ja) 1999-12-17 2010-07-21 ソニー株式会社 情報処理装置、情報処理方法、プログラム、および記録媒体、端末装置、並びに、システムおよびシステムの方法
EP1276857B1 (de) * 2000-04-07 2008-06-11 Novozymes A/S Einfangen von signalsequenzen
PT1356073E (pt) * 2000-10-20 2010-02-15 Bioneer As Método de fermentação aperfeiçoado para a produção de produtos genéticos heterólogos em bactérias do ácido láctico
FR2852325B1 (fr) 2003-03-13 2005-06-03 Acide nucleique dirigeant l'expression d'un polypeptide d'interet dans les glandes sericigenes posterieures d'un lepidoptere, et ses applications
EP1917278A2 (de) * 2005-08-26 2008-05-07 Cerenis Therapeutics Holding SA Zusammensetzungen und verfahren für die herstellung von apolipoprotein gene produkten in lactic acid bakterien
US20080293102A1 (en) * 2007-02-28 2008-11-27 Cerenis Therapeutics Holding, S.A. Compositions and methods for producing apolipoprotein
CN104480203A (zh) * 2014-12-08 2015-04-01 上海理工大学 一种快速检测乳酸乳球菌的引物及其检测方法和试剂盒
US11661447B2 (en) 2017-08-03 2023-05-30 The Cleveland Clinic Foundation Human β2-glycoprotein I expression
GB2569631B (en) 2017-12-21 2022-10-19 Kohler Mira Ltd A flow dispersion device
WO2024124138A2 (en) * 2022-12-08 2024-06-13 The Board Of Trustees Of The University Of Illinois A molecular toolkit for heterologous protein secretion in bacteroides species

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4914025A (en) * 1985-12-05 1990-04-03 Colin Manoil Export of intra-cellular substances
US5242821A (en) 1989-07-10 1993-09-07 Valio, Finnish Co-Operative Dairies' Association Lactococcus promoter and signal sequences for expression in bacteria
AU652920B2 (en) * 1990-08-30 1994-09-15 Valio Ltd. Promoter probe vectors, able to replicate in E.coli, B.subtilis, lactococci and lactobacillus as well as uses thereof
WO1994016086A1 (en) * 1992-12-30 1994-07-21 Bioteknologisk Institut Recombinant lactic acid bacterium containing an inserted promoter and method of constructing same
DE19521312A1 (de) * 1995-06-12 1996-12-19 Max Planck Gesellschaft Verfahren zur Identifizierung sekretorischer Gene aus Helicobacter pylori
NZ334521A (en) 1996-09-06 2000-03-27 Bioteknologisk Inst A lactic acid bacterial regulatable expression system
WO2000006736A2 (en) * 1998-07-27 2000-02-10 Microbial Technics Limited Nucleic acids and proteins from group b streptococcus

Also Published As

Publication number Publication date
AU772650B2 (en) 2004-05-06
CA2381184C (en) 2011-08-02
WO2001011060A2 (en) 2001-02-15
US7186815B2 (en) 2007-03-06
DK1206556T3 (da) 2007-05-21
CA2381184A1 (en) 2001-02-15
DE60033037T2 (de) 2007-07-19
NZ517079A (en) 2003-08-29
JP2003516119A (ja) 2003-05-13
US20040038263A1 (en) 2004-02-26
ES2276691T3 (es) 2007-07-01
JP4271396B2 (ja) 2009-06-03
EP1206556B1 (de) 2007-01-17
EP1206556A2 (de) 2002-05-22
AU6426700A (en) 2001-03-05
DE60033037D1 (de) 2007-03-08
WO2001011060A3 (en) 2001-08-23

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