ATE357529T1 - Methode und zusammensetzungen zur verbesserten polynucleotidsynthese - Google Patents

Methode und zusammensetzungen zur verbesserten polynucleotidsynthese

Info

Publication number: ATE357529T1
Authority: AT; Austria
Prior art keywords: blocking group; compositions; polynucleotide synthesis; synthesis; polynucleotide
Prior art date: 1999-09-22

Application number

AT00966814T

Other languages

English (en)

Inventor

Shuwei Yang

Original Assignee

Genecopoeia Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-09-22

Filing date

2000-09-22

Publication date

2007-04-15

2000-09-22 Application filed by Genecopoeia Inc filed Critical Genecopoeia Inc

2007-04-15 Application granted granted Critical

2007-04-15 Publication of ATE357529T1 publication Critical patent/ATE357529T1/de

Links

230000015572 biosynthetic process Effects 0.000 title abstract 4
108091033319 polynucleotide Proteins 0.000 title abstract 4
102000040430 polynucleotide Human genes 0.000 title abstract 4
239000002157 polynucleotide Substances 0.000 title abstract 4
238000003786 synthesis reaction Methods 0.000 title abstract 4
238000000034 method Methods 0.000 title abstract 3
239000000203 mixture Substances 0.000 title abstract 2
108091034117 Oligonucleotide Proteins 0.000 abstract 3
230000000903 blocking effect Effects 0.000 abstract 3
102000004190 Enzymes Human genes 0.000 abstract 2
108090000790 Enzymes Proteins 0.000 abstract 2
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
230000003321 amplification Effects 0.000 abstract 1
238000004458 analytical method Methods 0.000 abstract 1
238000001514 detection method Methods 0.000 abstract 1
238000003745 diagnosis Methods 0.000 abstract 1
238000003199 nucleic acid amplification method Methods 0.000 abstract 1
244000052769 pathogen Species 0.000 abstract 1
230000001717 pathogenic effect Effects 0.000 abstract 1
230000035945 sensitivity Effects 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Molecular Biology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biomedical Technology (AREA)
General Chemical & Material Sciences (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Immunology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)
Saccharide Compounds (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

AT00966814T 1999-09-22 2000-09-22 Methode und zusammensetzungen zur verbesserten polynucleotidsynthese ATE357529T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US09/404,258 US6274353B1 (en)	1999-09-22	1999-09-22	Method and compositions for improved polynucleotide synthesis

Publications (1)

Publication Number	Publication Date
ATE357529T1 true ATE357529T1 (de)	2007-04-15

Family

ID=23598857

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT00966814T ATE357529T1 (de)	1999-09-22	2000-09-22	Methode und zusammensetzungen zur verbesserten polynucleotidsynthese

Country Status (8)

Country	Link
US (2)	US6274353B1 (de)
EP (1)	EP1224295B1 (de)
JP (1)	JP2003510052A (de)
CN (2)	CN101649307B (de)
AT (1)	ATE357529T1 (de)
AU (1)	AU7710200A (de)
DE (1)	DE60034053D1 (de)
WO (1)	WO2001021813A1 (de)

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US6670127B2 (en)	1997-09-16	2003-12-30	Egea Biosciences, Inc.	Method for assembly of a polynucleotide encoding a target polypeptide
KR100809949B1 (ko) *	2001-02-15	2008-03-06	다카라 바이오 가부시키가이샤	염기 치환의 검출 방법
EP1275735A1 (de)	2001-07-11	2003-01-15	Roche Diagnostics GmbH	Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
US6667165B2 (en)	2001-11-13	2003-12-23	Eppendorf Ag	Method and compositions for reversible inhibition of thermostable polymerases
US6896727B2 (en) *	2002-06-28	2005-05-24	Seh America, Inc.	Method of determining nitrogen concentration within a wafer
WO2004003228A1 (en) *	2002-07-01	2004-01-08	Unisearch Limited	Genotyping method
US20060228786A1 (en) *	2003-02-06	2006-10-12	Salerno John C	Polymerase-based protocols for the introduction of deletions and insertions
US20060257876A1 (en) *	2003-02-06	2006-11-16	Rensselaer Polytechnis Institute	Polymerase-based protocols for generating chimeric and combinatorial...
US20060134624A1 (en) *	2003-02-06	2006-06-22	Salerno John C	Polymerase-based protocols for the introductions of deletions and insertions
WO2004072252A2 (en) *	2003-02-06	2004-08-26	Rensselaer Polytechnic Institute	Polymerase-based protocols for the introduction of deletions and insertions
WO2004090153A2 (en)	2003-04-01	2004-10-21	Eragen Biosciences,Inc.	Polymerase inhibitor and method of using same
US20050244847A1 (en) *	2003-11-26	2005-11-03	Eppendorf Ag	Methods and compositions for in vitro amplification of extrachromosomal nucleic acid
CN101426908B (zh) *	2004-06-09	2014-06-11	纽星实验室	可逆性修饰的热稳定酶组合物及其制备方法和使用方法
JP5558811B2 (ja) *	2006-06-01	2014-07-23	トリリンクバイオテクノロジーズ	核酸増幅のための化学的に修飾されたオリゴヌクレオチドプライマー
US8911948B2 (en)	2008-04-30	2014-12-16	Integrated Dna Technologies, Inc.	RNase H-based assays utilizing modified RNA monomers
US10227641B2 (en)	2008-04-30	2019-03-12	Integrated Dna Technologies, Inc.	RNase H-based assays utilizing modified RNA monomers
US9434988B2 (en)	2008-04-30	2016-09-06	Integrated Dna Technologies, Inc.	RNase H-based assays utilizing modified RNA monomers
EP3150727B1 (de) *	2008-04-30	2019-07-10	Integrated DNA Technologies Inc.	Rnase-h-basierte assays unter verwendung von modifizierten rna monomeren
CA2765167A1 (en) *	2009-06-12	2010-12-16	Nanectis Biotechnologies	A highly sensitive method for detection of viral hiv dna remaining after antiretroviral therapy of aids patients
EP2488664A4 (de) *	2009-10-13	2014-04-09	Syntezza Molecular Detection Israel Ltd	Verfahren und zusammensetzungen zur verstärkung von zielsequenzen aus nukleinsäureproben
US10378051B2 (en)	2011-09-29	2019-08-13	Illumina Cambridge Limited	Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
CN103205425B (zh) *	2012-01-17	2015-07-01	北京万达因生物医学技术有限责任公司	抑制引物非特异性扩增的反义干扰寡核苷酸
LT2825672T (lt) *	2012-03-13	2019-06-10	Swift Biosciences, Inc.	Būdai ir kompozicijos, skirti valdomam pagal dydį substrato polinukleotidų homopolimeriniam uodegų formavimui su nukleorūgščių polimeraze
AU2013381709A1 (en) *	2013-03-15	2015-10-01	Integrated Dna Technologies, Inc.	RNase H-based assays utilizing modified RNA monomers
WO2021120095A1 (zh) *	2019-12-19	2021-06-24	深圳华大生命科学研究院	抗聚合酶的单克隆抗体及其应用
US20240102067A1 (en) *	2022-09-26	2024-03-28	Illumina, Inc.	Resynthesis Kits and Methods
CN117070514B (zh) *	2023-10-16	2024-04-26	吉林凯莱英医药化学有限公司	非天然rna的制备方法及产品
CN117821422B (zh) *	2023-12-07	2024-11-08	北京擎科生物科技股份有限公司	核酸内切酶iv突变体及其应用

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ES2112302T3 (es) *	1991-10-11	1998-04-01	Behringwerke Ag	Metodo para producir un polinucleotido para uso en amplificacion con un unico cebador y oligonucleotidos que contienen fosforotioato como cebadores para la amplificacion de acidos nucleicos.
US5516664A (en)	1992-12-23	1996-05-14	Hyman; Edward D.	Enzymatic synthesis of repeat regions of oligonucleotides
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CA2176193A1 (en) *	1995-05-22	1996-11-23	John Wesley Backus	Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
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1999
- 1999-09-22 US US09/404,258 patent/US6274353B1/en not_active Expired - Fee Related
2000
- 2000-09-22 CN CN2009101511681A patent/CN101649307B/zh not_active Expired - Lifetime
- 2000-09-22 AT AT00966814T patent/ATE357529T1/de not_active IP Right Cessation
- 2000-09-22 AU AU77102/00A patent/AU7710200A/en not_active Abandoned
- 2000-09-22 CN CN00815973A patent/CN100590198C/zh not_active Expired - Fee Related
- 2000-09-22 DE DE60034053T patent/DE60034053D1/de not_active Expired - Lifetime
- 2000-09-22 EP EP00966814A patent/EP1224295B1/de not_active Expired - Lifetime
- 2000-09-22 WO PCT/US2000/026107 patent/WO2001021813A1/en not_active Ceased
- 2000-09-22 JP JP2001525371A patent/JP2003510052A/ja active Pending
2001
- 2001-05-14 US US09/853,701 patent/US6699981B2/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
DE60034053D1 (de)	2007-05-03
WO2001021813A1 (en)	2001-03-29
CN101649307A (zh)	2010-02-17
JP2003510052A (ja)	2003-03-18
US6274353B1 (en)	2001-08-14
CN101649307B (zh)	2011-11-30
US20020182686A1 (en)	2002-12-05
EP1224295B1 (de)	2007-03-21
CN100590198C (zh)	2010-02-17
AU7710200A (en)	2001-04-24
US6699981B2 (en)	2004-03-02
CN1408025A (zh)	2003-04-02
EP1224295A1 (de)	2002-07-24

Legal Events

Date	Code	Title	Description
2007-09-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
DE60034053D1 (de)	2007-05-03	Methode und zusammensetzungen zur verbesserten polynucleotidsynthese
Brightwell et al.	1998	Development of internal controls for PCR detection ofBacillus anthracis
US5849497A (en)	1998-12-15	Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
Ross et al.	2001	Mitochondrial DNA polymorphism: its role in longevity of the Irish population
AR040710A1 (es)	2005-04-13	Evento de maiz pv-zmir 13 (mon863), plantas, composiciones y metodos para la deteccion del mismo
AR085952A2 (es)	2013-11-06	Polinucleotidos cebadores
EP2366803A3 (de)	2011-11-23	Zusammensetzungen und Verfahren zum Nacheis des West Nil Viruses.
DE60112746D1 (de)	2005-09-22	Kälteempfindliche dna-polymerasemutanten
ES2118593T3 (es)	1998-09-16	Analogos de nucleotidos de pteridina como sondas fluorescentes de adn.
HUP0101385A2 (hu)	2001-08-28	Eljárás nukleinsavak detektálására PCR-módszerrel
EA200401354A1 (ru)	2005-02-24	Химерный олигонуклеотидный праймер для способа амплификации нуклеиновой кислоты; продукт для амплификации нуклеиновой кислоты; зонд для способа детекции нуклеиновой кислоты-мишени; материал с иммобилизованной амплифицированной нуклеиновой кислотой и способ его получения; способ определения нуклеотидной последовательности
DE69527776D1 (de)	2002-09-19	Nachweis humaner papillomviren mit nukleinsäüre amplifikation die generelle primer verwendet
ATE532877T1 (de)	2011-11-15	Nukleinsäuremoleküle zum nachweis von bakterien und phylogenetischen einheiten von bakterien
DE60132924D1 (de)	2008-04-03	Zusammensetzungen und methoden zur detektion von humanem immundefizienz virus 2 (hiv-2)
DE59609841D1 (de)	2002-12-12	Verfahren zur Gattungs- und speziesspezifische Identifizierung von Legionellen
DE69427876D1 (de)	2001-09-06	Methode zur detektion einer gezielten nukleinsäure
Gasser et al.	2001	Mutation scanning analysis of sequence heterogeneity in the second internal transcribed spacer (rDNA) within some members of the Hypodontus macropi (Nematoda: Strongyloidea) complex
Kai et al.	2006	Development of single-cell PCR methods for the Raphidophyceae
DE69616102D1 (de)	2001-11-29	Amplikationsprimern und Nukleinsäuresonden zum Nachweis von Coccidioides immites
Van Doorn et al.	2009	Robust detection and identification of multiple oomycetes and fungi in environmental samples by using a novel cleavable padlock probe-based ligation detection assay
DE69943134D1 (de)	2011-02-24	Nukleinsäureamplifizierung und detektion von mykobacterium-spezies
Dyer et al.	1998	Detecting genetic variation in exotic earthworms, Aporrectodea spp.(Lumbricidae), in Australian soils using RAPD markers
AR027256A1 (es)	2003-03-19	Deteccion de microorganismos relacionados con la fermentacion
DE50213026D1 (de)	2009-01-02	Tifikation in einer probe
EP0872561A3 (de)	1999-11-17	Neisseria gonorrhoae spezifische Oligonukleotide