ATE359369T1 - Rns-abhängige rns-polymerase, die vorzugsweise anhand einer rna-matrix funktioniert und ein promoter-abhängiger transkriptionsprozess , der diese rns-abhängige rns-polymerase benutzt. - Google Patents

Rns-abhängige rns-polymerase, die vorzugsweise anhand einer rna-matrix funktioniert und ein promoter-abhängiger transkriptionsprozess , der diese rns-abhängige rns-polymerase benutzt.

Info

Publication number
ATE359369T1
ATE359369T1 AT98917263T AT98917263T ATE359369T1 AT E359369 T1 ATE359369 T1 AT E359369T1 AT 98917263 T AT98917263 T AT 98917263T AT 98917263 T AT98917263 T AT 98917263T AT E359369 T1 ATE359369 T1 AT E359369T1
Authority
AT
Austria
Prior art keywords
rna
dependent
rna polymerase
matrix
polymerase
Prior art date
Application number
AT98917263T
Other languages
English (en)
Inventor
Valerie Cheynet-Sauvion
Nadege Arnaud-Barbe
Guy Oriol
William Mcallister
Bernard Mandrand
Francois Mallet
Original Assignee
Bio Merieux
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Merieux filed Critical Bio Merieux
Application granted granted Critical
Publication of ATE359369T1 publication Critical patent/ATE359369T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/127—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT98917263T 1997-04-04 1998-03-27 Rns-abhängige rns-polymerase, die vorzugsweise anhand einer rna-matrix funktioniert und ein promoter-abhängiger transkriptionsprozess , der diese rns-abhängige rns-polymerase benutzt. ATE359369T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9704166A FR2761695B1 (fr) 1997-04-04 1997-04-04 Arn polymerase fonctionnant preferentiellement sur matrice d'arn, sa preparation et son utilisation

Publications (1)

Publication Number Publication Date
ATE359369T1 true ATE359369T1 (de) 2007-05-15

Family

ID=9505551

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98917263T ATE359369T1 (de) 1997-04-04 1998-03-27 Rns-abhängige rns-polymerase, die vorzugsweise anhand einer rna-matrix funktioniert und ein promoter-abhängiger transkriptionsprozess , der diese rns-abhängige rns-polymerase benutzt.

Country Status (10)

Country Link
US (2) US6828127B2 (de)
EP (1) EP0972051B1 (de)
JP (1) JP4004072B2 (de)
AT (1) ATE359369T1 (de)
AU (1) AU7052898A (de)
CA (1) CA2286343C (de)
DE (1) DE69837535T2 (de)
ES (1) ES2285766T3 (de)
FR (1) FR2761695B1 (de)
WO (1) WO1998045449A1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4832694B2 (ja) * 2000-03-07 2011-12-07 アクゾ・ノベル・エヌ・ベー 増加した熱安定性を有するｒｎａポリメラーゼ変異体
US7074558B2 (en) * 2001-06-07 2006-07-11 Pbi Technology, Inc. Nucleic acid amplification using an RNA polymerase and DNA/RNA mixed polymer intermediate products
FR2828509B1 (fr) 2001-08-08 2003-11-21 D Lascaud Ets Panneau isolant thermique
AU2005322131B2 (en) * 2004-12-23 2011-11-10 Global Life Sciences Solutions Usa Llc Ligation-based RNA amplification
CN107460177B (zh) * 2016-06-06 2021-08-17 张海生 可利用化学修饰核苷酸的rna聚合酶突变体
CN118773233A (zh) * 2017-03-28 2024-10-15 味之素株式会社 生产rna的方法
WO2021007233A1 (en) 2019-07-10 2021-01-14 The Board Of Trustees Of The Leland Stanford Junior University Rna replication using transcription polymerases
CN115768470A (zh) * 2020-03-03 2023-03-07 火箭疫苗股份公司 用于疫苗生产以防范冠状病毒的全合成的长链核酸
US20240066116A1 (en) * 2021-03-03 2024-02-29 Rocketvax Ag Fully synthetic, long-chain nucleic acid for vaccine production to protect against coronaviruses
CN113584014B (zh) * 2021-07-27 2023-01-20 上海兆维科技发展有限公司 一种rna的制备方法、合成蛋白质的方法以及转录反应液

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE659881C (de) 1936-04-25 1938-05-14 I G Farbenindustrie Akt Ges Verfahren zur Herstellung von Dehydrobinaphthylendiimin und seinen Substitutionsprodukten
WO1990006376A1 (en) 1988-12-05 1990-06-14 The Salk Institute For Biological Studies Target nucleic acid amplification/detection systems
US5122457A (en) 1989-10-19 1992-06-16 Schering Corporation Expression systems utilizing bacteriophage t7 promoters, gene sequences, and t7 rna polymerase
FR2708288B1 (fr) * 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
FR2714062B1 (fr) * 1993-12-22 1996-02-16 Bio Merieux Promoteur modifié pour ARN polymérase, sa préparation et ses applications.
JP3996639B2 (ja) 1995-06-07 2007-10-24 ジェン−プローブ・インコーポレーテッド 生成物の増幅後レベルから核酸標的配列の増幅前レベルを決定するための方法とキット
CA2233309A1 (en) * 1995-09-27 1997-04-03 Emory University Recombinant hepatitis c virus rna replicase

Also Published As

Publication number Publication date
EP0972051A1 (de) 2000-01-19
US7595180B2 (en) 2009-09-29
CA2286343A1 (fr) 1998-10-15
AU7052898A (en) 1998-10-30
WO1998045449A1 (fr) 1998-10-15
DE69837535T2 (de) 2007-12-13
FR2761695A1 (fr) 1998-10-09
FR2761695B1 (fr) 1999-09-24
JP4004072B2 (ja) 2007-11-07
ES2285766T3 (es) 2007-11-16
DE69837535D1 (de) 2007-05-24
US20020119449A1 (en) 2002-08-29
JP2001507942A (ja) 2001-06-19
CA2286343C (fr) 2008-12-23
US6828127B2 (en) 2004-12-07
EP0972051B1 (de) 2007-04-11
US20050069933A1 (en) 2005-03-31

Similar Documents

Publication Publication Date Title
MX9705961A (es) Polimerasa de adn termoestable, modificada.
KR960703174A (ko) 자기장 순환식 반응방법(magnetic cycle reaction)
Liu et al. Biosensing by tandem reactions of structure switching, nucleolytic digestion, and DNA amplification of a DNA assembly
ATE209258T1 (de) Verwendung von rna-polymerase zur verbesserung von nukeinsaeure-amplifikationsverfahren
KR890013184A (ko) 핵산을 증폭시키는 방법
WO2001071042A3 (en) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
ATE284973T1 (de) Nukleinsäuretypisierung durch polymeraseverlängerung von oligonukleotiden unter verwendung von terminator-mischungen
DE69837535D1 (de) Rns-abhängige rns-polymerase, die vorzugsweise anhand einer rna-matrix funktioniert und ein promoter-abhängiger transkriptionsprozess , der diese rns-abhängige rns-polymerase benutzt.
KR900018376A (ko) 리보핵산(rna)의 합성방법
WO2005089508A3 (en) Dna sequence detection by limited primer extension
DK110691A (da) Selv-vedligeholdende sekvensreplikationssystem
DE69432419D1 (de) Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
EP0359789A1 (de) Verstärkung und nachweis von nukleinsäuresequenzen.
GB2377220B (en) Polynucleotide analysis using combinatorial PCR
DE69407187D1 (de) Identifizierung von salmonella durch die polymerase-ketten-reaktion
Sadler et al. Fast and flexible synthesis of combinatorial libraries for directed evolution
WO2004097003A3 (en) Multiply-primed amplification of nucleic acid sequences
TW329476B (en) Method for detecting contaminant DNA in a protein product and kits used for the same purpose
Kojima et al. In vitro selection of DNA binding sites for transcription factor, PhaR, from Paracoccus denitrificans using genetic library on microbeads and flow cytometry
DE69637684D1 (de) Dna moleküle
ATE470710T1 (de) Auf nasba basiertes detektionsverfahren zur detektion des tuberkelbazillus
DE602004025815D1 (de) Verfahren zur amplifikation polymerer nukleinsäuren
Bektas et al. Variant (E484K) ALERT-Ligation-Dependent Loop-Mediated Isothermal Amplification
DE69618957D1 (de) Dna-polymerase verlängungs assay
Guo et al. Universal primer applications for pyrosequencing®

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties