ATE360093T1 - Reaktionsgemisch und verfahren für rt-pcr mittels einer homopolymeren nukleinsäure - Google Patents
Reaktionsgemisch und verfahren für rt-pcr mittels einer homopolymeren nukleinsäureInfo
- Publication number
- ATE360093T1 ATE360093T1 AT00109078T AT00109078T ATE360093T1 AT E360093 T1 ATE360093 T1 AT E360093T1 AT 00109078 T AT00109078 T AT 00109078T AT 00109078 T AT00109078 T AT 00109078T AT E360093 T1 ATE360093 T1 AT E360093T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acids
- nucleic acid
- homopolymeric
- pcr
- reaction mixture
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 7
- 102000039446 nucleic acids Human genes 0.000 title abstract 7
- 108020004707 nucleic acids Proteins 0.000 title abstract 7
- 229920001519 homopolymer Polymers 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 239000011541 reaction mixture Substances 0.000 title abstract 2
- 238000003757 reverse transcription PCR Methods 0.000 title 1
- 238000010240 RT-PCR analysis Methods 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 102100034343 Integrase Human genes 0.000 abstract 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 239000013626 chemical specie Substances 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
- 229920002477 rna polymer Polymers 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/304,452 US6300069B1 (en) | 1999-05-03 | 1999-05-03 | Generation and amplification of nucleic acids from ribonucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE360093T1 true ATE360093T1 (de) | 2007-05-15 |
Family
ID=23176577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00109078T ATE360093T1 (de) | 1999-05-03 | 2000-05-02 | Reaktionsgemisch und verfahren für rt-pcr mittels einer homopolymeren nukleinsäure |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US6300069B1 (de) |
| EP (1) | EP1050587B1 (de) |
| JP (1) | JP4777497B2 (de) |
| AT (1) | ATE360093T1 (de) |
| DE (1) | DE60034396T2 (de) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070161010A1 (en) * | 1998-08-04 | 2007-07-12 | Gjerde Douglas T | Methods and compositions for mutation analysis |
| US20030175723A1 (en) * | 1998-08-04 | 2003-09-18 | Gjerde Douglas T. | Methods and compositions for mutation analysis |
| US20030175709A1 (en) * | 2001-12-20 | 2003-09-18 | Murphy George L. | Method and system for depleting rRNA populations |
| PT2143803T (pt) * | 2002-09-03 | 2016-07-14 | Quanta Biosciences Inc | Composições e métodos melhorados para a síntese de adnc |
| CN1316036C (zh) * | 2003-01-06 | 2007-05-16 | 徐定邦 | 一种以聚合酶链式反应为基础的基因定量方法 |
| EP1594982A4 (de) * | 2003-01-29 | 2006-04-12 | Quanta Biosciences Inc | Verfahren und zusammensetzungen zum nachweis und zur quantifizierung von rna |
| WO2004104196A1 (ja) * | 2003-05-20 | 2004-12-02 | G & G Science Co., Ltd. | 緩衝剤組成物 |
| JPWO2004104196A1 (ja) * | 2003-05-20 | 2006-07-20 | G&Gサイエンス株式会社 | 緩衝剤組成物 |
| JP2005110621A (ja) * | 2003-10-10 | 2005-04-28 | Aisin Seiki Co Ltd | 核酸増幅方法及び核酸増幅用試薬キット |
| WO2005054435A2 (en) * | 2003-11-26 | 2005-06-16 | Eppendorf Ag | Methods and compositions for in vitro amplification of extrachromosomal nucleic acid |
| US7824857B2 (en) | 2003-12-02 | 2010-11-02 | Musc Foundation For Research Development | Methods and compositions for diagnosing epithelial cell cancer |
| US7981616B2 (en) | 2004-02-27 | 2011-07-19 | Musc Foundation For Research Development | Enhanced detection of RNA using a panel of truncated gene-specific primers for reverse transcription |
| US8158772B2 (en) * | 2004-04-16 | 2012-04-17 | National Institute Of Agrobiological Sciences | Oligonucleotide sequences that identify species of animal |
| WO2006017150A2 (en) | 2004-07-09 | 2006-02-16 | University Of Pittsburgh - Of The Commonwealth System Of Higher Educatiion | Identification of markers in lung and breast cancer |
| AU2005314431B2 (en) * | 2004-12-11 | 2011-02-17 | Cytogenix, Inc. | Cell free biosynthesis of high-quality nucleic acid and uses thereof |
| WO2007019444A2 (en) * | 2005-08-05 | 2007-02-15 | Euclid Diagnostics Llc | Subtractive separation and amplification of non-ribosomal transcribed rna (nrrna) |
| DE102005047617A1 (de) * | 2005-10-05 | 2007-04-19 | Qiagen Gmbh | Polymerase-Kettenreaktions-Verfahren unter Einsatz einer DNA-Polymerase mit Proofreading-Eigenschaft |
| DE102006038113A1 (de) * | 2006-08-14 | 2008-02-21 | Qiagen Gmbh | Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität |
| US8980561B1 (en) | 2006-08-22 | 2015-03-17 | Los Alamos National Security, Llc. | Nucleic acid detection system and method for detecting influenza |
| US20090047673A1 (en) | 2006-08-22 | 2009-02-19 | Cary Robert B | Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids |
| GB0701253D0 (en) | 2007-01-23 | 2007-02-28 | Diagnostics For The Real World | Nucleic acid amplification and testing |
| US8153401B2 (en) * | 2007-05-17 | 2012-04-10 | Applied Biosystems, Llc | Method for direct amplification from crude nucleic acid samples |
| EP2191008B1 (de) * | 2007-08-13 | 2013-01-09 | Qiagen GmbH | Verfahren zur synthese einer cdna in einer probe in einer enzymatischen reaktion |
| US8338094B2 (en) | 2007-11-27 | 2012-12-25 | Bio-Rad Laboratories, Inc. | Reduced inhibition of one-step RT-PCR |
| WO2009137059A1 (en) | 2008-05-05 | 2009-11-12 | Los Alamos National Security, Llc | Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control |
| SI2294225T1 (sl) | 2008-06-30 | 2015-04-30 | Life Technologies Corporation | Postopek za neposredno amplifikacijo iz vzorcev surove nukleinske kisline |
| US8278041B2 (en) * | 2008-10-27 | 2012-10-02 | Infraegis, Inc. | Compositions and methods for detecting food-borne pathogens |
| US9394333B2 (en) | 2008-12-02 | 2016-07-19 | Wave Life Sciences Japan | Method for the synthesis of phosphorus atom modified nucleic acids |
| MX342945B (es) | 2009-07-06 | 2016-10-18 | Ontorii Inc * | Profármacos de ácido nucleico novedosos y métodos de uso de los mismos. |
| DK2620428T3 (da) | 2010-09-24 | 2019-07-01 | Wave Life Sciences Ltd | Asymmetrisk hjælpegruppe |
| JP5879667B2 (ja) | 2011-04-20 | 2016-03-08 | メサ バイオテック,インク. | 核酸の検出および同定のための一体型デバイス |
| WO2012170907A2 (en) | 2011-06-08 | 2012-12-13 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
| CN106518696B (zh) | 2011-06-08 | 2019-06-14 | 生命技术公司 | 用于pcr系统的新型去污剂的设计和开发 |
| US9605019B2 (en) | 2011-07-19 | 2017-03-28 | Wave Life Sciences Ltd. | Methods for the synthesis of functionalized nucleic acids |
| EP2559764A1 (de) * | 2011-08-17 | 2013-02-20 | Qiagen GmbH | Zusammensetzung und Verfahren für reverse Transkriptase-Polymerase-Kettenreaktion (RT-PCR )mit einem anionischen Polymer |
| SG11201500239VA (en) | 2012-07-13 | 2015-03-30 | Wave Life Sciences Japan | Asymmetric auxiliary group |
| KR20240148947A (ko) | 2012-07-13 | 2024-10-11 | 웨이브 라이프 사이언시스 리미티드 | 키랄 제어 |
| SG11201500243WA (en) | 2012-07-13 | 2015-04-29 | Shin Nippon Biomedical Lab Ltd | Chiral nucleic acid adjuvant |
| EP3063129B1 (de) | 2013-10-25 | 2019-04-17 | Life Technologies Corporation | Neuartige verbindungen zur verwendung in pcr-systemen und anwendungen davon |
| WO2015108047A1 (ja) | 2014-01-15 | 2015-07-23 | 株式会社新日本科学 | 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤 |
| EP3095460A4 (de) | 2014-01-15 | 2017-08-23 | Shin Nippon Biomedical Laboratories, Ltd. | Chirales nukleinsäureadjuvans mit antiallergischer wirkung und antiallergikum |
| WO2015108048A1 (ja) | 2014-01-15 | 2015-07-23 | 株式会社新日本科学 | 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤 |
| ES2917473T3 (es) | 2014-01-16 | 2022-07-08 | Wave Life Sciences Ltd | Diseño quiral |
| WO2016106129A2 (en) | 2014-12-23 | 2016-06-30 | Ge Healthcare Uk Limited | Methods and reagents for reverse-transcription polymerase chain reaction |
| WO2016106113A1 (en) | 2014-12-23 | 2016-06-30 | Ge Healthcare Uk Limited | Methods and reagents for reverse-transcription polymerase chain reaction |
| US10968478B2 (en) | 2014-12-23 | 2021-04-06 | Global Life Sciences Solutions Operations UK Ltd | Methods and reagents for reverse-transcription polymerase chain reaction |
| WO2016172724A1 (en) | 2015-04-24 | 2016-10-27 | Mesa Biotech, Inc. | Fluidic test cassette |
| EP4022083B1 (de) * | 2019-08-30 | 2024-07-03 | Life Technologies Corporation | Zusammensetzungen und verfahren für multiplex-pcr und genetischen analyse |
| WO2021177041A1 (ja) * | 2020-03-02 | 2021-09-10 | 東洋紡株式会社 | 非特異的な核酸増幅を抑制する方法 |
| US20250059575A1 (en) | 2020-05-26 | 2025-02-20 | Qiagen Beverly Llc | Polymerase enzyme |
| JP7188645B2 (ja) * | 2020-07-29 | 2022-12-13 | 東洋紡株式会社 | 非特異的な核酸増幅を抑制する方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT787807E (pt) * | 1991-08-27 | 2003-08-29 | Hoffmann La Roche | Iniciadores e sondas para a deteccao da hepatite c |
| DE69734263T2 (de) * | 1996-07-12 | 2006-07-13 | Toyo Boseki K.K. | Verfahren zur Isolierung von Ribonucleinsäuren. |
| JP3681405B2 (ja) * | 1997-02-21 | 2005-08-10 | タカラバイオ株式会社 | 癌関連遺伝子 |
| ATE486961T1 (de) | 1997-04-03 | 2010-11-15 | Life Technologies Corp | Verfahren und produkte zur reversen transkription gekoppelt mit polymerasekettenreaktion (rt-pcr) |
-
1999
- 1999-05-03 US US09/304,452 patent/US6300069B1/en not_active Expired - Lifetime
-
2000
- 2000-05-02 JP JP2000133664A patent/JP4777497B2/ja not_active Expired - Lifetime
- 2000-05-02 DE DE60034396T patent/DE60034396T2/de not_active Expired - Lifetime
- 2000-05-02 AT AT00109078T patent/ATE360093T1/de not_active IP Right Cessation
- 2000-05-02 EP EP00109078A patent/EP1050587B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP2000342287A (ja) | 2000-12-12 |
| EP1050587A2 (de) | 2000-11-08 |
| EP1050587B1 (de) | 2007-04-18 |
| JP4777497B2 (ja) | 2011-09-21 |
| DE60034396T2 (de) | 2008-01-10 |
| DE60034396D1 (de) | 2007-05-31 |
| US6300069B1 (en) | 2001-10-09 |
| EP1050587A3 (de) | 2004-02-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |