ATE360093T1 - Reaktionsgemisch und verfahren für rt-pcr mittels einer homopolymeren nukleinsäure - Google Patents
Reaktionsgemisch und verfahren für rt-pcr mittels einer homopolymeren nukleinsäureInfo
- Publication number
- ATE360093T1 ATE360093T1 AT00109078T AT00109078T ATE360093T1 AT E360093 T1 ATE360093 T1 AT E360093T1 AT 00109078 T AT00109078 T AT 00109078T AT 00109078 T AT00109078 T AT 00109078T AT E360093 T1 ATE360093 T1 AT E360093T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acids
- nucleic acid
- homopolymeric
- pcr
- reaction mixture
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 7
- 102000039446 nucleic acids Human genes 0.000 title abstract 7
- 108020004707 nucleic acids Proteins 0.000 title abstract 7
- 229920001519 homopolymer Polymers 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 239000011541 reaction mixture Substances 0.000 title abstract 2
- 238000003757 reverse transcription PCR Methods 0.000 title 1
- 238000010240 RT-PCR analysis Methods 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 102100034343 Integrase Human genes 0.000 abstract 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 239000013626 chemical specie Substances 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
- 229920002477 rna polymer Polymers 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/304,452 US6300069B1 (en) | 1999-05-03 | 1999-05-03 | Generation and amplification of nucleic acids from ribonucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE360093T1 true ATE360093T1 (de) | 2007-05-15 |
Family
ID=23176577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00109078T ATE360093T1 (de) | 1999-05-03 | 2000-05-02 | Reaktionsgemisch und verfahren für rt-pcr mittels einer homopolymeren nukleinsäure |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US6300069B1 (de) |
| EP (1) | EP1050587B1 (de) |
| JP (1) | JP4777497B2 (de) |
| AT (1) | ATE360093T1 (de) |
| DE (1) | DE60034396T2 (de) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030175723A1 (en) * | 1998-08-04 | 2003-09-18 | Gjerde Douglas T. | Methods and compositions for mutation analysis |
| US20070161010A1 (en) * | 1998-08-04 | 2007-07-12 | Gjerde Douglas T | Methods and compositions for mutation analysis |
| US20030175709A1 (en) * | 2001-12-20 | 2003-09-18 | Murphy George L. | Method and system for depleting rRNA populations |
| ATE447040T1 (de) * | 2002-09-03 | 2009-11-15 | Quanta Biosciences Inc | Verbesserte zusammensetzungen und verfahren für die cdna-synthese |
| CN1316036C (zh) * | 2003-01-06 | 2007-05-16 | 徐定邦 | 一种以聚合酶链式反应为基础的基因定量方法 |
| EP1594982A4 (de) * | 2003-01-29 | 2006-04-12 | Quanta Biosciences Inc | Verfahren und zusammensetzungen zum nachweis und zur quantifizierung von rna |
| WO2004104196A1 (ja) * | 2003-05-20 | 2004-12-02 | G & G Science Co., Ltd. | 緩衝剤組成物 |
| JPWO2004104196A1 (ja) * | 2003-05-20 | 2006-07-20 | G&Gサイエンス株式会社 | 緩衝剤組成物 |
| JP2005110621A (ja) * | 2003-10-10 | 2005-04-28 | Aisin Seiki Co Ltd | 核酸増幅方法及び核酸増幅用試薬キット |
| US20050244847A1 (en) * | 2003-11-26 | 2005-11-03 | Eppendorf Ag | Methods and compositions for in vitro amplification of extrachromosomal nucleic acid |
| WO2005055804A2 (en) | 2003-12-02 | 2005-06-23 | Musc Foundation For Research Development | Methods and compositions for diagnosing epithelial cell cancer |
| US7981616B2 (en) | 2004-02-27 | 2011-07-19 | Musc Foundation For Research Development | Enhanced detection of RNA using a panel of truncated gene-specific primers for reverse transcription |
| US8158772B2 (en) * | 2004-04-16 | 2012-04-17 | National Institute Of Agrobiological Sciences | Oligonucleotide sequences that identify species of animal |
| AU2005271961B8 (en) | 2004-07-09 | 2011-11-24 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Identification of markers in esophageal cancer, colon cancer, head and neck cancer and melanoma |
| WO2006063355A2 (en) * | 2004-12-11 | 2006-06-15 | Cytogenix , Inc. | Cell free biosynthesis of high-quality nucleic acid and uses thereof |
| US20090137415A1 (en) * | 2005-08-05 | 2009-05-28 | Euclid Diagnostics Llc | SUBTRACTIVE SEPARATION AND AMPLIFICATION OF NON-RIBOSOMAL TRANSCRIBED RNA (nrRNA) |
| DE102005047617A1 (de) * | 2005-10-05 | 2007-04-19 | Qiagen Gmbh | Polymerase-Kettenreaktions-Verfahren unter Einsatz einer DNA-Polymerase mit Proofreading-Eigenschaft |
| DE102006038113A1 (de) * | 2006-08-14 | 2008-02-21 | Qiagen Gmbh | Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität |
| US20090047673A1 (en) | 2006-08-22 | 2009-02-19 | Cary Robert B | Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids |
| US8980561B1 (en) | 2006-08-22 | 2015-03-17 | Los Alamos National Security, Llc. | Nucleic acid detection system and method for detecting influenza |
| GB0701253D0 (en) | 2007-01-23 | 2007-02-28 | Diagnostics For The Real World | Nucleic acid amplification and testing |
| US8153401B2 (en) * | 2007-05-17 | 2012-04-10 | Applied Biosystems, Llc | Method for direct amplification from crude nucleic acid samples |
| EP2191008B1 (de) * | 2007-08-13 | 2013-01-09 | Qiagen GmbH | Verfahren zur synthese einer cdna in einer probe in einer enzymatischen reaktion |
| JP5702148B2 (ja) | 2007-11-27 | 2015-04-15 | バイオ−ラッド ラボラトリーズ,インコーポレイティド | 1ステップrt−pcrの阻害低減 |
| CA2723536C (en) | 2008-05-05 | 2017-06-13 | Los Alamos National Security, Llc | Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control |
| EP2294225B8 (de) | 2008-06-30 | 2015-02-18 | Life Technologies Corporation | Verfahren zur direkten amplifikation aus rohen nukeinsäureproben |
| CA2779197C (en) | 2008-10-27 | 2021-06-29 | Infraegis, Inc. | Method of removing polymerase chain reaction (pcr) inhibitors from a sample using .beta.-cyclodextrin and activated charcoal coated with bentonite |
| BRPI0923225A2 (pt) | 2008-12-02 | 2016-10-04 | Chiralgen Ltd | metodo para sintese de acidos nucleicos modificados no atomo de fosforo |
| EP2451461A4 (de) | 2009-07-06 | 2013-05-29 | Ontorii Inc | Neuartige nukleinsäure-prodrugs und verwendungsverfahren dafür |
| DK2620428T3 (da) | 2010-09-24 | 2019-07-01 | Wave Life Sciences Ltd | Asymmetrisk hjælpegruppe |
| CN103649333B (zh) | 2011-04-20 | 2017-02-15 | 梅撒生物科技公司 | 用于核酸检测和鉴定的集成装置 |
| US9567628B2 (en) | 2011-06-08 | 2017-02-14 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
| EP3461807B1 (de) | 2011-06-08 | 2023-07-12 | Life Technologies Corporation | Design und entwicklung neuartiger reinigungsmittel zur verwendung in pcr-systemen |
| CN107365339A (zh) | 2011-07-19 | 2017-11-21 | 波涛生命科学有限公司 | 合成官能化核酸的方法 |
| EP2559764A1 (de) | 2011-08-17 | 2013-02-20 | Qiagen GmbH | Zusammensetzung und Verfahren für reverse Transkriptase-Polymerase-Kettenreaktion (RT-PCR )mit einem anionischen Polymer |
| WO2014010250A1 (en) | 2012-07-13 | 2014-01-16 | Chiralgen, Ltd. | Asymmetric auxiliary group |
| DK2872147T3 (da) | 2012-07-13 | 2023-02-20 | Wave Life Sciences Ltd | Fremgangsmåde til fremstilling af kirale oligonukleotider |
| SG11201500243WA (en) | 2012-07-13 | 2015-04-29 | Shin Nippon Biomedical Lab Ltd | Chiral nucleic acid adjuvant |
| JP6742238B2 (ja) | 2013-10-25 | 2020-08-19 | ライフ テクノロジーズ コーポレーション | Pcrシステムにおいて使用するための新規化合物及びその用途 |
| EP3095459A4 (de) | 2014-01-15 | 2017-08-23 | Shin Nippon Biomedical Laboratories, Ltd. | Chirales nukleinsäureadjuvans mit antitumorwirkung und antitumormittel |
| EP3095461A4 (de) | 2014-01-15 | 2017-08-23 | Shin Nippon Biomedical Laboratories, Ltd. | Chirales nukleinsäure-adjuvans mit immunitätsinduktionswirkung und immunitätsinduktionsaktivator |
| WO2015108046A1 (ja) | 2014-01-15 | 2015-07-23 | 株式会社新日本科学 | 抗アレルギー作用を有するキラル核酸アジュバンド及び抗アレルギー剤 |
| EP3094728B1 (de) | 2014-01-16 | 2022-03-09 | Wave Life Sciences Ltd. | Chirales design |
| US10968478B2 (en) | 2014-12-23 | 2021-04-06 | Global Life Sciences Solutions Operations UK Ltd | Methods and reagents for reverse-transcription polymerase chain reaction |
| WO2016106129A2 (en) | 2014-12-23 | 2016-06-30 | Ge Healthcare Uk Limited | Methods and reagents for reverse-transcription polymerase chain reaction |
| US10858694B2 (en) | 2014-12-23 | 2020-12-08 | Global Life Sciences Solutions Operations UK Ltd | Methods and reagents for reverse-transcription polymerase chain reaction |
| AU2016253147B2 (en) | 2015-04-24 | 2021-07-22 | Mesa Biotech, Inc. | Fluidic test cassette |
| US20220403446A1 (en) * | 2019-08-30 | 2022-12-22 | Life Technologies Corporation | Compositions and methods for multiplex rt-pcr and genetic analysis |
| WO2021177041A1 (ja) * | 2020-03-02 | 2021-09-10 | 東洋紡株式会社 | 非特異的な核酸増幅を抑制する方法 |
| US20250059575A1 (en) | 2020-05-26 | 2025-02-20 | Qiagen Beverly Llc | Polymerase enzyme |
| CN116529367A (zh) * | 2020-07-29 | 2023-08-01 | 东洋纺株式会社 | 抑制非特异性核酸扩增的方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0529493T3 (da) * | 1991-08-27 | 1998-08-24 | Hoffmann La Roche | Fremgangsmåder og reagenser til hepatitis C-detektion |
| DE69734263T2 (de) * | 1996-07-12 | 2006-07-13 | Toyo Boseki K.K. | Verfahren zur Isolierung von Ribonucleinsäuren. |
| AU6229098A (en) * | 1997-02-21 | 1998-09-09 | Takara Shuzo Co., Ltd. | Cancer-associated genes |
| EP1705253B1 (de) | 1997-04-03 | 2010-11-03 | Life Technologies Corporation | Verfahren und Produkte zur reversen Transkription gekoppelt mit Polymerasekettenreaktion (RT-PCR) |
-
1999
- 1999-05-03 US US09/304,452 patent/US6300069B1/en not_active Expired - Lifetime
-
2000
- 2000-05-02 AT AT00109078T patent/ATE360093T1/de not_active IP Right Cessation
- 2000-05-02 JP JP2000133664A patent/JP4777497B2/ja not_active Expired - Lifetime
- 2000-05-02 EP EP00109078A patent/EP1050587B1/de not_active Expired - Lifetime
- 2000-05-02 DE DE60034396T patent/DE60034396T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1050587A2 (de) | 2000-11-08 |
| US6300069B1 (en) | 2001-10-09 |
| JP2000342287A (ja) | 2000-12-12 |
| DE60034396T2 (de) | 2008-01-10 |
| JP4777497B2 (ja) | 2011-09-21 |
| EP1050587B1 (de) | 2007-04-18 |
| DE60034396D1 (de) | 2007-05-31 |
| EP1050587A3 (de) | 2004-02-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |