ATE362986T1 - High fidelity thermostabile ligase und ihre verwendung - Google Patents

High fidelity thermostabile ligase und ihre verwendung

Info

Publication number
ATE362986T1
ATE362986T1 AT99956776T AT99956776T ATE362986T1 AT E362986 T1 ATE362986 T1 AT E362986T1 AT 99956776 T AT99956776 T AT 99956776T AT 99956776 T AT99956776 T AT 99956776T AT E362986 T1 ATE362986 T1 AT E362986T1
Authority
AT
Austria
Prior art keywords
ligase
thermostable ligase
high fidelity
reaction process
fidelity thermostable
Prior art date
Application number
AT99956776T
Other languages
English (en)
Inventor
Francis Barany
Weiguo Cao
Jie Tong
Original Assignee
Cornell Res Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Res Foundation Inc filed Critical Cornell Res Foundation Inc
Application granted granted Critical
Publication of ATE362986T1 publication Critical patent/ATE362986T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dowels (AREA)
  • Vessels, Lead-In Wires, Accessory Apparatuses For Cathode-Ray Tubes (AREA)
  • Confectionery (AREA)
AT99956776T 1998-10-30 1999-10-29 High fidelity thermostabile ligase und ihre verwendung ATE362986T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10646198P 1998-10-30 1998-10-30

Publications (1)

Publication Number Publication Date
ATE362986T1 true ATE362986T1 (de) 2007-06-15

Family

ID=22311532

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99956776T ATE362986T1 (de) 1998-10-30 1999-10-29 High fidelity thermostabile ligase und ihre verwendung

Country Status (7)

Country Link
EP (1) EP1127135B1 (de)
JP (1) JP4598275B2 (de)
AT (1) ATE362986T1 (de)
AU (1) AU776820B2 (de)
CA (1) CA2348776C (de)
DE (1) DE69936147T2 (de)
WO (1) WO2000026381A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6949370B1 (en) 1998-10-30 2005-09-27 Cornell Research Foundation, Inc. High fidelity thermostable ligase and uses thereof
AU2001253332A1 (en) * 2000-04-14 2001-10-30 Livecapital, Inc. Online credit services brokering
CA2535922A1 (en) * 2003-09-23 2005-04-07 University Of Missouri Methods of synthesizing polynucleotides using thermostable enzymes
CA3049989A1 (en) 2017-02-10 2018-08-16 Zymergen Inc. A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts
EP3821020A4 (de) 2018-08-15 2022-05-04 Zymergen Inc. Anwendungen von crispri in metabolischem engineering mit hohem durchsatz
CN115803433A (zh) 2020-07-17 2023-03-14 凯杰贝佛利有限公司 序列偏倚降低的热稳定连接酶
CN117247911B (zh) * 2023-08-22 2024-06-07 武汉爱博泰克生物科技有限公司 大肠杆菌dna连接酶突变体及其在毕赤酵母中表达纯化方法
WO2025197400A1 (ja) * 2024-03-19 2025-09-25 デンカ株式会社 高い正確性で、ミスマッチ塩基対を有さない配列を接合するリガーゼ
WO2026087220A1 (en) 2024-10-22 2026-04-30 F. Hoffmann-La Roche Ag Preparation of dna libraries for next generation sequencing using y-adapters modified with phosphorothioate bonds
WO2026093331A1 (en) 2024-10-28 2026-05-07 F. Hoffmann-La Roche Ag Hairpin adapter constructs and primers to generate next-generation sequencing library molecules

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0528882T3 (da) * 1990-05-03 2008-01-14 Cornell Res Foundation Inc DNA-amplifikationssystem til påvisning af genetiske sygdomme ved hjælp af termostabil ligase
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction

Also Published As

Publication number Publication date
WO2000026381A3 (en) 2000-11-09
CA2348776C (en) 2008-03-25
DE69936147T2 (de) 2008-01-24
CA2348776A1 (en) 2000-05-11
AU1331000A (en) 2000-05-22
DE69936147D1 (de) 2007-07-05
JP4598275B2 (ja) 2010-12-15
JP2002528121A (ja) 2002-09-03
EP1127135A2 (de) 2001-08-29
AU776820B2 (en) 2004-09-23
WO2000026381A2 (en) 2000-05-11
EP1127135B1 (de) 2007-05-23

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Legal Events

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