ES2180416A1 - Procedimiento para la preparacion de mezclas de reaccion estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reaccion y kits que las contienen. - Google Patents

Procedimiento para la preparacion de mezclas de reaccion estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reaccion y kits que las contienen.

Info

Publication number
ES2180416A1
ES2180416A1 ES200100569A ES200100569A ES2180416A1 ES 2180416 A1 ES2180416 A1 ES 2180416A1 ES 200100569 A ES200100569 A ES 200100569A ES 200100569 A ES200100569 A ES 200100569A ES 2180416 A1 ES2180416 A1 ES 2180416A1
Authority
ES
Spain
Prior art keywords
mixtures
reaction mixtures
enzyme
aqueous solution
totally
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES200100569A
Other languages
English (en)
Other versions
ES2180416B1 (es
Inventor
De Sarabia Rosado Pedro Franco
Lopez Gemma Limones
Seiz Antonio Madejon
Alberdi M Dolores Marin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotools Biotechnological and Medical Laboratories SA
Original Assignee
Biotools Biotechnological and Medical Laboratories SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ES200100569A priority Critical patent/ES2180416B1/es
Application filed by Biotools Biotechnological and Medical Laboratories SA filed Critical Biotools Biotechnological and Medical Laboratories SA
Priority to CA002408857A priority patent/CA2408857A1/en
Priority to PCT/ES2002/000109 priority patent/WO2002072002A2/es
Priority to KR1020027015148A priority patent/KR20030036178A/ko
Priority to JP2002570962A priority patent/JP4448280B2/ja
Priority to AU2002246125A priority patent/AU2002246125B2/en
Priority to BRPI0204479-0B1A priority patent/BR0204479B1/pt
Priority to EP02714205.8A priority patent/EP1374827B1/en
Priority to IL152757A priority patent/IL152757A/en
Priority to US10/292,848 priority patent/US7919294B2/en
Publication of ES2180416A1 publication Critical patent/ES2180416A1/es
Application granted granted Critical
Publication of ES2180416B1 publication Critical patent/ES2180416B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procedimiento para la preparación de mezclas de reacción estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reacción y kits que las contienen. El procedimiento comprende poner en contacto en un mismo contenedor una solución acuosa de una mezcla de reacción que comprende, al menos, una enzima, con una solución acuosa de una mezcla estabilizante compuesta por (i) al menos, un agente protector frente a la desecación, (ii) al menos, un inhibidor de la reacción de condensación entre grupos carbonilo o carboxilo y grupos amino o fosfato, y (iii) al menos, un polímero inerte capaz de generar una estructura en forma de malla que impide la movilidad de los reactivos desecados; y retirar la totalidad o parte del agua contenida en la solución acuosa resultante. De aplicación en la realización de reacciones enzimáticas, por ejemplo, amplificación, secuenciación y caracterización de ácidos nucleicos, ensayos de hibridación y análisis de restricción.
ES200100569A 2001-03-12 2001-03-12 Procedimiento para la preparacion de mezclas de reaccion estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reaccion y kits que las contienen. Expired - Fee Related ES2180416B1 (es)

Priority Applications (10)

Application Number Priority Date Filing Date Title
ES200100569A ES2180416B1 (es) 2001-03-12 2001-03-12 Procedimiento para la preparacion de mezclas de reaccion estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reaccion y kits que las contienen.
IL152757A IL152757A (en) 2001-03-12 2002-03-11 Process for preparing stabilized, partially dried reaction mixtures containing at least one enzyme, reaction mixtures, and kits containing these mixtures
KR1020027015148A KR20030036178A (ko) 2001-03-12 2002-03-11 적어도 한가지의 효소를 함유하는 완전히 또는 부분적으로건조되고 안정화된 반응 혼합물, 이를 제조하는 방법 및이를 포함하는 키트
JP2002570962A JP4448280B2 (ja) 2001-03-12 2002-03-11 部分的に乾燥した１つ以上の酵素を含む安定化された混合物の製造方法、混合物、および混合物を含有するキット
AU2002246125A AU2002246125B2 (en) 2001-03-12 2002-03-11 Process for preparing stabilized reaction mixtures which are partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures
BRPI0204479-0B1A BR0204479B1 (pt) 2001-03-12 2002-03-11 processo para preparar misturas de reaÇço estabilizadas que sço total ou parcialmente secas, compreendendo pelo menos uma enzima, misturas de reaÇço e kits contendo as referidas misturas
CA002408857A CA2408857A1 (en) 2001-03-12 2002-03-11 Method for preparing stabilised reaction mixtures, which are totally or partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures
PCT/ES2002/000109 WO2002072002A2 (es) 2001-03-12 2002-03-11 Procedimiento para la preparación de mezclas de reacción estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reacción y kits que las contienen
EP02714205.8A EP1374827B1 (en) 2001-03-12 2002-03-11 Method for preparing stabilised reaction mixtures, which are totally or partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures
US10/292,848 US7919294B2 (en) 2001-03-12 2002-11-12 Process for preparing stabilized reaction mixtures which are partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200100569A ES2180416B1 (es) 2001-03-12 2001-03-12 Procedimiento para la preparacion de mezclas de reaccion estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reaccion y kits que las contienen.

Publications (2)

Publication Number Publication Date
ES2180416A1 true ES2180416A1 (es) 2003-02-01
ES2180416B1 ES2180416B1 (es) 2004-06-01

Family

ID=8497049

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200100569A Expired - Fee Related ES2180416B1 (es) 2001-03-12 2001-03-12 Procedimiento para la preparacion de mezclas de reaccion estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reaccion y kits que las contienen.

Country Status (10)

Country Link
US (1) US7919294B2 (es)
EP (1) EP1374827B1 (es)
JP (1) JP4448280B2 (es)
KR (1) KR20030036178A (es)
AU (1) AU2002246125B2 (es)
BR (1) BR0204479B1 (es)
CA (1) CA2408857A1 (es)
ES (1) ES2180416B1 (es)
IL (1) IL152757A (es)
WO (1) WO2002072002A2 (es)

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Also Published As

Publication number Publication date
CA2408857A1 (en) 2002-09-19
ES2180416B1 (es) 2004-06-01
BR0204479A (pt) 2004-02-17
US20030119042A1 (en) 2003-06-26
JP4448280B2 (ja) 2010-04-07
WO2002072002A3 (es) 2002-11-28
US7919294B2 (en) 2011-04-05
IL152757A (en) 2009-02-11
EP1374827A2 (en) 2004-01-02
EP1374827B1 (en) 2016-12-21
JP2004519242A (ja) 2004-07-02
AU2002246125B2 (en) 2008-02-07
IL152757A0 (en) 2003-06-24
KR20030036178A (ko) 2003-05-09
WO2002072002A2 (es) 2002-09-19
BR0204479B1 (pt) 2013-07-16

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