ATE364613T1 - Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension - Google Patents

Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension

Info

Publication number
ATE364613T1
ATE364613T1 AT01997500T AT01997500T ATE364613T1 AT E364613 T1 ATE364613 T1 AT E364613T1 AT 01997500 T AT01997500 T AT 01997500T AT 01997500 T AT01997500 T AT 01997500T AT E364613 T1 ATE364613 T1 AT E364613T1
Authority
AT
Austria
Prior art keywords
separating
cell suspension
extrachromosomal dna
extrachromosomal
dna
Prior art date
Application number
AT01997500T
Other languages
English (en)
Inventor
Henry Charlton
Alex Eon-Duval
Robert Macduff
Original Assignee
Glaxo Group Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glaxo Group Ltd filed Critical Glaxo Group Ltd
Application granted granted Critical
Publication of ATE364613T1 publication Critical patent/ATE364613T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT01997500T 2000-11-21 2001-11-20 Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension ATE364613T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0028361.4A GB0028361D0 (en) 2000-11-21 2000-11-21 Method of separating extra chromosomal dna from other cellular components

Publications (1)

Publication Number Publication Date
ATE364613T1 true ATE364613T1 (de) 2007-07-15

Family

ID=9903579

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01997500T ATE364613T1 (de) 2000-11-21 2001-11-20 Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension

Country Status (13)

Country Link
US (1) US20040076980A1 (de)
EP (1) EP1335926B1 (de)
JP (1) JP4422406B2 (de)
AR (1) AR031482A1 (de)
AT (1) ATE364613T1 (de)
AU (1) AU2002223841A1 (de)
CA (1) CA2428885A1 (de)
DE (1) DE60128945T2 (de)
ES (1) ES2287189T3 (de)
GB (1) GB0028361D0 (de)
MY (1) MY129812A (de)
TW (1) TWI289142B (de)
WO (1) WO2002042317A2 (de)

Families Citing this family (30)

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GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
AU2006211216B2 (en) 2005-01-31 2011-02-03 Merck Sharp & Dohme Llc Purification process for plasmid DNA
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
EP3318248B1 (de) 2009-12-01 2019-04-10 Translate Bio, Inc. Ausgabe von mrna zur vermehrung von proteinen und enzymen bei humangenetischen erkrankungen
AT509622A1 (de) * 2010-04-08 2011-10-15 Greiner Bio One Gmbh Verfahren und kit zur trennung viraler und prokaryontischer von eukaryontischen nukleinsäuren
US10883100B2 (en) 2011-03-29 2021-01-05 Phynexus, Inc Devices and methods for plasmid purification
US10597652B2 (en) * 2011-03-29 2020-03-24 Phynexus, Inc. Methods and devices for nucleic acid purification
US11274292B2 (en) 2011-03-29 2022-03-15 Phynexus, Inc. Devices and methods for plasmid purification
RU2013154295A (ru) 2011-06-08 2015-07-20 Шир Хьюман Дженетик Терапис, Инк. КОМПОЗИЦИИ ЛИПИДНЫХ НАНОЧАСТИЦ И СПОСОБЫ ДЛЯ ДОСТАВКИ мРНК
WO2013185067A1 (en) 2012-06-08 2013-12-12 Shire Human Genetic Therapies, Inc. Nuclease resistant polynucleotides and uses thereof
CN107753954A (zh) 2012-12-13 2018-03-06 免疫医疗公司 功效改进且毒性降低的抗体与sn‑38的免疫缀合物的剂量
WO2015012904A2 (en) * 2012-12-13 2015-01-29 Immunomedics, Inc. Antibody-sn-38 immunoconjugates with a cl2a linker
US9931417B2 (en) 2012-12-13 2018-04-03 Immunomedics, Inc. Antibody-SN-38 immunoconjugates with a CL2A linker
RS57739B1 (sr) 2013-03-14 2018-12-31 Translate Bio Inc Kompozicije cftr irnk i postupci i upotrebe u vezi sa njima
JP6586075B2 (ja) 2013-03-14 2019-10-02 トランスレイト バイオ， インコーポレイテッド メッセンジャーｒｎａの精製方法
AU2014293670B2 (en) * 2013-07-23 2019-08-01 Immunomedics, Inc. Antibody-SN-38 immunoconjugates with a CL2A linker
CN106413811A (zh) 2013-10-22 2017-02-15 夏尔人类遗传性治疗公司 精氨基琥珀酸合成酶缺乏症的mrna疗法
JP6506749B2 (ja) 2013-10-22 2019-04-24 シャイアー ヒューマン ジェネティック セラピーズ インコーポレイテッド フェニルケトン尿症のためのｍＲＮＡ療法
US9850269B2 (en) 2014-04-25 2017-12-26 Translate Bio, Inc. Methods for purification of messenger RNA
EP4660315A3 (de) 2015-05-29 2026-03-18 CureVac Manufacturing GmbH Verfahren zur herstellung und reinigung von rna mit mindestens einem schritt der tangentialen strömungsfiltration
AU2017257254B2 (en) 2016-04-27 2022-02-24 Immunomedics, Inc. Efficacy of anti-Trop-2-SN-38 antibody drug conjugates for therapy of tumors relapsed/refractory to checkpoint inhibitors
EA201991747A1 (ru) 2017-02-27 2020-06-04 Транслейт Био, Инк. НОВАЯ КОДОН-ОПТИМИЗИРОВАННАЯ мРНК CFTR
EP3600283A4 (de) 2017-03-27 2020-12-16 Immunomedics, Inc. Behandlung von trop-2-exprimierendem triple-negativem brustkrebs mit sacituzumab govitecan und einem rad51-hemmer
US10799597B2 (en) 2017-04-03 2020-10-13 Immunomedics, Inc. Subcutaneous administration of antibody-drug conjugates for cancer therapy
MX2019013752A (es) 2017-05-16 2020-07-20 Translate Bio Inc Tratamiento de la fibrosis quística mediante el suministro de arnm que codifica cftr optimizado en codones.
KR102950141B1 (ko) 2018-08-24 2026-04-07 트랜슬레이트 바이오 인코포레이티드 전령 rna의 정제 방법
WO2020106946A1 (en) 2018-11-21 2020-05-28 Translate Bio, Inc. TREATMENT OF CYSTIC FIBROSIS BY DELIVERY OF NEBULIZED mRNA ENCODING CFTR
WO2020160087A1 (en) * 2019-01-29 2020-08-06 Flagship Pioneering Innovations V, Inc. Methods of separating long polynucleotides from a composition
EP4031662A1 (de) 2019-09-20 2022-07-27 Translate Bio, Inc. Mrna-codierendes technisches cftr
CN116790578B (zh) * 2023-08-22 2023-12-12 赛奥斯博生物科技(北京)有限公司 一种基于碱裂解法快速纯化质粒的生产工艺

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US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
AU1444997A (en) * 1996-02-06 1997-08-28 Boehringer Mannheim Gmbh Process for preparing purified nucleic acid and the use thereof
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
EP0853123A1 (de) * 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
US6268492B1 (en) * 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
CA2378944A1 (en) * 1999-07-23 2001-02-01 Genentech, Inc. Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration
US6747132B2 (en) * 2000-11-29 2004-06-08 Apex Biosciences, Inc. Methods for the synthesis of a modified hemoglobin solution

Also Published As

Publication number Publication date
CA2428885A1 (en) 2002-05-30
WO2002042317A2 (en) 2002-05-30
GB0028361D0 (en) 2001-01-03
US20040076980A1 (en) 2004-04-22
MY129812A (en) 2007-05-31
TWI289142B (en) 2007-11-01
DE60128945T2 (de) 2008-02-28
JP2004514428A (ja) 2004-05-20
EP1335926B1 (de) 2007-06-13
ES2287189T3 (es) 2007-12-16
AR031482A1 (es) 2003-09-24
DE60128945D1 (de) 2007-07-26
WO2002042317A3 (en) 2003-02-06
JP4422406B2 (ja) 2010-02-24
EP1335926A2 (de) 2003-08-20
AU2002223841A1 (en) 2002-06-03

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